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Citations for Bio-Rad :
5551 - 5600 of 5608 citations for Mouse Leucine Rich Pentatricopeptide Repeat Containing LRPPRC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... as detailed in “RNA/DNA extraction and sequencing.” Kars RNA expression was measured in each tumor using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad Cat. No. 1725150) and the Quantstudio 6 Flex System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg RNA was transferred into the cDNA Ecodry Premix Kit prior to the quantitative PCR program being run on ABI QuantStudio 3 with iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, 1725121). Each reaction proceeded at 50 °C for 2 min ...
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bioRxiv - Biochemistry 2024Quote: ... The total protein concentration of pooled saliva samples was measured to be approximately 20 mg/mL using the Bradford assay kit (Bio-Rad, Hercules, CA, USA) and bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 uL of the extracted genomic DNA were used for qPCR and HRM analysis using the IQ SYBR® Green Supermix kit (BioRad, Cat no. 1708882). Mutation positive male sperm were used to fertilize wildtype eggs which were saved in culture beakers and raised until next spawn ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg of total RNA was reverse-transcribed to single-stranded complementary DNA (cDNA) using iScript cDNA Synthesis Kit (Bio-Rad Hercules, CA, USA). RT-qPCR was run on Lightcycler 480 (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... Total protein concentrations of the cell extracts were determined using the Bio-Rad Protein Assay Kit according to the manufacturer’s instructions (Bio-Rad Laboratories, Hercules, CA, USA) using BSA as standard.
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bioRxiv - Plant Biology 2023Quote: ... The qPCR was performed using the DyNAmo ColorFlash SYBR Green qPCR Kit (Thermo-scientific) with CFX96 Touch Real-Time PCR Detection System (Bio-Rad Hercules, CA, USA) according to the manufacturer’s protocol (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... One microgram of high-quality RNA was used for the first-strand cDNA synthesis by using an iScript™ cDNA synthesis kit (Bio-Rad Laboratories, USA). Primer specificity was ensured by performing a BLASTN search of each sequence against the Solanum lycopersicum ITAGv2.40 database (Supplementary Table S1) ...
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bioRxiv - Immunology 2023Quote: ... The viral genome of the H7N1 virus was quantified using qRT-PCR that was conducted with the Bio-Rad iTaq™ Universal SYBR ® Green One-Step Kit (BioRad, California, USA) using primers designed for the detection of the M gene as published elsewhere 60.
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bioRxiv - Genetics 2023Quote: ... RNA samples were normalised to the same concentration of 250 ng/μL and used to synthesise the complementary DNA using an iScript cDNA synthesis kit (Bio-Rad, catalogue no.: 1708891) following the manufacturer’s instructions ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... Mini kit (Macherery-Magel, Germany) and used to prepare complementary DNA (cDNA) using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad, Hercules, CA, USA) on ABS Proflex Thermal cycler (Thermo-fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of RNA were then used for cDNA synthesis with the iScript™ Advanced cDNA Synthesis Kit (Bio-Rad Laboratories, catalog number 1725038). RT-qPCR analysis was performed on the CFX384 Touch™ Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized using 100 ng of purified total RNA in a 20 μL volume reaction with an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA), as described in (52) ...
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bioRxiv - Physiology 2024Quote: ... Purified RNA was then used for cDNA synthesis via reverse transcription as per protocol by iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). All PCR reactions (25 µL ...
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bioRxiv - Genetics 2024Quote: ... The membranes were then incubated with ECL (Easysee Western Blot Kit, China) and visualized with an Imaging System (Bio-Rad, Universal Hood II, USA).
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bioRxiv - Microbiology 2024Quote: Proteins separated by SDS-PAGE were transferred to a PVDF membrane using the Trans-Blot® Turbo RTA Mini PVDF Transfer Kit (Bio-Rad Laboratories, Inc.). Membranes after activating with methanol were blocked with bovine serum albumin (Amresco ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to a nitrocellulose membrane using the Trans-Blot® Turbo™ RTA Mini Nitrocellulose Transfer Kit and Trans-Blot© Turbo™ Transfer System (Bio-Rad). Antibody detection was carried out with the SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Precision Plus Protein™ Dual Color Standards and Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit were purchased from Bio-Rad (Hercules, CA). Antibodies against Phospho-c-Raf (Ser338) ...
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bioRxiv - Cell Biology 2019Quote: ... and then converted to units of fmol/mg of total protein added to an assay point (as measured by Bio-Rad DC Protein Assay Kit). Equilibrium dissociation constants (Kd ...
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Glutathione S-transferase: A Candidate Gene for Berry Color in Muscadine Grapes (Vitis rotundifolia)bioRxiv - Genetics 2020Quote: ... Total RNA from berry skins and seeds was used for the synthesis of first strand cDNA using iScript cDNA synthesis kit (Bio-Rad Laboratories Inc., Hercules, CA) and diluted 1:10 for qPCR reactions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the 1st cDNA was synthesized from 200 ng of total RNA of stomach origin by using oligo-dT primers and an iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA). The sequences of primers for cDNA cloning were designed based on the genome resource (see Table 2) ...
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bioRxiv - Microbiology 2020Quote: ... Levels of the following 27 cytokines were analyzed using a BioPlex Pro™ Human Cytokine 27-plex Assay kit (#M500KCAF0Y, Bio-Rad, Hercules, CA, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and cDNA was synthesized from total RNA with the High Capacity cDNA Reverse Transcription kit (Applied Biosystems; Thermo Fisher. Real-time PCR was performed with iTaq Universal SYBR Green Supermix (Biorad; Cat No. 1725121) or SYBR Green PCR master mix (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... 100 ng aliquots of RNA before and after depletion were subjected to qPCR using the iTaq™ Universal SYBR® Green One-Step kit (Bio-Rad, Hercules, CA) [22] ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed with C-kit variant 1 forward primer 5’-CAACAAAGAGCAAATCCATCCC-3’ and reverse primer 5’-CATCACAATAATGCACATCATGCC-3’ with iQ SYBR Green supermix (BioRad, Hercules, CA, Cat No. 1708882). The PCR program was as follows ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was generated by reverse transcription of the total RNA samples with iScriptRT Advanced cDNA Synthesis Kit for RT-PCR (Bio-Rad, Hercules, CA; #170–8891;). qRT-PCR was performed in triplicates using the iTaqTM Universal SYBR® Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: Commercially available TaqMan assays (Table S2) for droplet digital PCR were prepared using the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad, Hercules, CA, USA; 1864022). Each 20-μL reaction mix consisted of 10 μL ddPCR Supermix for probes (no dUTP ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were resolved in 10% SDS-polyacrylamide gels and transferred to nitrocellulose membranes using the Trans-Blot Turbo RTA Midi Nitrocellulose Transfer Kit (#1704271 from Bio-Rad Laboratories, Hercules, CA, USA). Phosphorylation of MAPKs Slt2 and Fus3 was detected using a rabbit antiPhospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Neuroscience 2020Quote: ... Uptake was normalized to total mg of protein per sample measured via Bio-Rad DC protein assay kit (5000112 Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Cell Biology 2019Quote: ... Gels were transferred using Trans-Blot® Turbo™ RTA Mini PVDF Transfer Kit and the Trans-Blot® Turbo™ Transfer system (Bio-Rad). Membranes were blocked with Odyssey® Blocking Buffer (LI-COR ...
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bioRxiv - Neuroscience 2021Quote: ... was resuspended in 1 ml of PBS/Ca+/Mg+ buffer and protein content was quantified by DC protein assay kit (Bio-Rad Laboratory, Hercules, CA, USA). 500 µg of synaptosomes were incubated 60 min at 4 °C with sulfo-NHS-biotin (1.5 mg/ml ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... aliquots of the samples were organized in a 96-well PCR plate and reverse transcribed to cDNA using the iScript™ Reverse Transcription Supermix kit and protocol (Bio-Rad Laboratories, Hercules, CA). The resulting 96-well plate with cDNA was used as source quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Genetics 2021Quote: ... equal amounts of cDNA were synthesized from 300 ng of total RNA for each sample by using the iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA). Thirty nanograms of cDNA per reaction were analyzed for semi-qPCR using the SsoAdvanced™ Universal SYBR® Green Supermix Kit (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: Total RNA from snap-frozen IECs was isolated with peqGOLD RNAPure™ (Erlangen, Germany) and reverse transcription was performed using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, California, USA). Following ...
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bioRxiv - Immunology 2021Quote: ... For T84 cells: 250 ng of total RNA was reverse transcribed into cDNA using the iScript™ cDNA Synthesis kit (BioRad Laboratories, Catalog number 1708891). The reaction contained a mixture of 1 μl Reverse Transcriptase ...
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bioRxiv - Immunology 2022Quote: ... produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-Rad, Marnes-La-Coquette, France). Mouse IgG1 isotype control was purchased from Miltenyi Biotec (Bergisch Gladbach ...
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bioRxiv - Physiology 2022Quote: mRNA expression was analyzed by quantitative RT–PCR as previously described (49) using cDNA synthesis kits and SYBR green mix from Bio-Rad (Cat# 1708897 and 1725121) performed with the StepOne Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA synthesis of 500 ng of RNA in a final volume of 20 μL was performed using iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Bio-Rad MyiQ was used to quantify the DNA concentration of the library using the Kapa iCycler qPCR kit (Bio-Rad Laboratories, Hercules, CA, USA). Sequencing was performed on the Illumina NextSeq 500 Platform (Illumina ...
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bioRxiv - Immunology 2024Quote: ... Viral genome was quantified using a standard curve of known viral titer following qRT-PCR with iTaq Universal SYBR Green One-Step Kit (BioRad, cat#172-5151, Hercules, CA) and the following primers ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were then incubated with Omni-ECL™Femto Light Chemiluminescence Kit (Epizyme, China) and captured using the Molecular Imager® Gel Doc™ XR System (Bio-Rad, USA). The antibodies used were as follows ...
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bioRxiv - Molecular Biology 2023Quote: To prepare the tubing for gene gun delivery one tubing and 25 mg of Au particles with 1 µm Ø was used from Helios Gene Gun Optimization Kit (Bio-Rad, Hercules, CA, USA). 10 µg of the synthesized RNA transcript was used four labeling Au particles ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentrations were analyzed using a Quick Start Bradford Protein Assay Kit with bovine serum albumin as a standard (Bio-Rad Laboratories, Hercules, CA, USA). The samples were standardized by diluting in the diluent ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantitative PCR was performed using a KOD SYBR qPCR kit (TOYOBO) on a CFX96 Real-Time PCR System (Bio-Rad Laboratories, Hercules, CA, USA). PCR conditions were as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting total protein was quantified using the Bio-Rad DC Protein Assay Kit according to the manufacturer’s recommended protocol (Bio-Rad, Cat#5000111, Hercules, CA, USA). Samples and standards were read on a BioTek Synergy HT plate reader after a 15 min incubation period ...
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bioRxiv - Neuroscience 2024Quote: mRNA expression was analyzed by quantitative RT–PCR as previously described using cDNA synthesis kits and SYBR green mix from Bio-Rad (Cat# 1708897 and 1725121) performed with the StepOne Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Equal amounts of RNA for all samples in Fig 3N were simultaneously transcribed into cDNA using iScript cDNA synthesis kit (BIO-RAD, Hercules, CA, cat#170-8891) according to the manufacturer’s protocol within 7 days of the initial dissections to preserve sample quality ...
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bioRxiv - Cancer Biology 2020Quote: ... blots were developed using an ECL Ultra kit (New Cell & Molecular Biotech Co. Ltd, China) and images were captured using a ChemiDocTM XRS + Imager (Bio-Rad Laboratories, Inc., Hercules, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression levels of above-mentioned genes were estimated using iTaq™ Universal SYBR® Green One-step qRT-PCR kit (#1725151, Bio-Rad Laboratories Inc., USA) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... This sequence was amplified from B6J genomic DNA extracted from spleen tissue using iProof High-Fidelity PCR kit (BIO-RAD, Hercules, CA, USA; Cat# 172-5330) with the following primers containing XhoI and HindIII restriction enzyme sites in the sense and antisense primers respectively ...