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Citations for Bio-Rad :
5501 - 5550 of 5608 citations for Mouse Leucine Rich Pentatricopeptide Repeat Containing LRPPRC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Messenger RNA expression was assessed with 150 to 500 ng of total RNA using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, USA, #1708891) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... SYBR qPCR Master Mix kit (Q711, Vazyme, Nanjing, China) and a Bio-Rad CFX96 real-time PCR detection system (Bio-Rad, Hercules, USA) were used for qRT-PCR ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cDNA synthesis was performed for 57 samples using 500 ng of RNA per sample and the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Inc.). qPCR primers for the abovementioned genes were designed as described in Ahi & Sefc ...
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bioRxiv - Immunology 2023Quote: ... where absolute quantification was done by the QX200 Droplet Digital PCR System in combination with the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, United States). The limit of detection was calculated to be 10 copies per reaction.
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in cell culture supernatants was determined using the Magnetic Luminex Performance Assay (Human Base Kit A; R&D Systems, coupled with the Bio-Plex 200 (Bio-Rad). The trimmed median value was used to derive the standard curve and calculate sample concentrations.
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bioRxiv - Immunology 2023Quote: ... The supernatant was transferred to a new microcentrifuge tube and protein concentration was determined by using a DC protein assay kit (BioRad, Cat# 500-0116). Cell lysates (500-1000 μg ...
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bioRxiv - Biochemistry 2023Quote: ... total RNA (1 µg) was taken from the cDNA preparation using iScript™ cDNA Synthesis Kit (#1708891, Bio-Rad Laboratories, Hercules, CA, USA). Prior to cDNA synthesis ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolation of total RNA was performed using the Aurum™ Total RNA Mini-Kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). Total RNA concentration and quality were determined using a Nanodrop 1000 (ThermoFisher) ...
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bioRxiv - Systems Biology 2023Quote: ... Japan) and quantitative PCR using a KOD SYBR qPCR kit (TOYOBO) with CFX96 Real-Time PCR System (Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... The preparation of the genomic DNA plugs was carried out according to the CHEF genomic DNA plug kit (Bio-Rad, Hercules, CA, USA). The DNA templates of sgRNA-actF and sgRNA-actR were generated by overlap extension of sgRNA-actF/sgRNA-actR and guide RNA-F plus guide RNA-R ...
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bioRxiv - Neuroscience 2023Quote: ... We performed the reverse transcription (RT) reaction with 200 ng/µl of total RNA with the iScript gDNA clear advanced kit (Bio-Rad 172-5035) and the real time qPCR was performed with the Sso Advanced SYBR Green® Supermix (Bio-Rad 172-5270 ...
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bioRxiv - Microbiology 2023Quote: ... The fungal burden of each fungus ball was determined by measuring the galactomannan (GM) content in the supernatant [43] using the Platelia Aspergillus enzyme immunoassay kit (Bio-Rad, Hercules, CA) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... 1 µg RNA was synthesized to cDNA by reverse-transcription using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA; item # 1708891). Real-time PCR was performed in 10 µl reactions containing 5 µl SsoFast EvaGreen Supermix with Low ROX (Bio-Rad ...
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bioRxiv - Pathology 2024Quote: ... Quantitative PCR was performed to quantify Lrp2 mRNA abundance in the kidney using SsoFast™ EvaGreen® Supermix kits (Cat # 172-5204; Bio-Rad) on a Bio-Rad CFX96 cycler ...
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bioRxiv - Immunology 2024Quote: Cytokine secretion was analyzed from cleared PDEC culture supernatants using Bio-Plex Pro Human Cytokine 27-plex assay kit (Bio-Rad, cat. M500KCAF0Y) and Bio-Plex 200 System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... followed by real-time RT-PCR targeting highly conserved regions of the IAV matrix (M) (10) using iTaq Universal Probes One-Step Kit (Bio-Rad, 1725141, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The anti-HBs concentrations of the DBS samples were then tested using the Monolisa Anti-HBs PLUS kit (Bio-Rad, Hercules, CA, USA) with a 2.00 mIU/mL lower limit of detection (LoD ...
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bioRxiv - Immunology 2024Quote: ... and RNA was isolated by column purification (ZymoResearch R2050) and cDNA was synthesized using the iScript Reverse Transcriptase kit (Bio-Rad, Hercules, CA). qPCR was performed using SYBR green PCR master mix (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: Reverse transcription for qPCR was either performed using gene-specific reverse priming with the iScript™ Select cDNA Synthesis Kit (Bio-Rad #1708897) or using NEB LunaScript RT SuperMix kit (NEB #E3010L) ...
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bioRxiv - Cell Biology 2020Quote: ... RNA template (1 μg) was prepared and reverse transcription reaction was performed with iScript Advanced cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA) according to the procedure described in the product’s instruction manual ...
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bioRxiv - Molecular Biology 2021Quote: Total protein was extracted from SSLs using 350 μL of RIPA buffer and was purified using the Ready Prep 2-D cleanup kit (Bio-Rad, Hercules, CA, USA); the protein concentration was measured using the BCA protein assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... Total RNA (410 ng) was reverse transcribed into cDNA using an iScript™ Synthesis Kit on a T100™ thermal cycler (BIO-RAD, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Target proteins were detected by chemiluminescence with Clarity™ (Beta-actin and GFP) and Clarity Max™ (SP) Western ECL Substrate kit (Bio-Rad®) on the Odyssey FC system (LI-COR) ...
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bioRxiv - Cancer Biology 2020Quote: ... One μg RNA was reverse-transcribed using oligo(dT)18 and iScript Advanced cDNA synthesis kit for RT-qPCR (Bio-Rad Laboratories; Hercules, CA). Il1r1/IL1R1 and Gapdh/GAPDH qPCR was performed using specific primers and Lightcycler 480 Sybr Green I Master (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... the cDNA was synthesised using the Bio-Rad iScript™ cDNA Synthesis Kit (25 x 20 µl reactions #1708890, Bio-Rad Laboratories Inc., USA), while the qPCR reaction was performed using the Applied Biosystems PowerUp™ SYBR™ Green Master Mix (A25741 ...
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bioRxiv - Microbiology 2021Quote: ... The amount of viral RNA expressed as TCID50 equivalent per insert (TCID50e/insert) was quantified by RT-qPCR using iTaq universal probes one-step kit (Bio-Rad, catalogue no. 1725141), and a commercial mix of primers for N gene (forward primer 5’-GACCCCAAAATCAGCGAAAT-3’ ...
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bioRxiv - Neuroscience 2022Quote: ... and cDNAs were synthesized from 200 ng of RNA of each sample using iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad, Hercules, CA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Chemiluminescence reaction was initiated with Western Bright Quantum Western blotting detection kit and measured in a ChemiDocTM imaging system (BIO-RAD, Hercules, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... and their CWD status was independently identified utilizing the Bio-Rad TeSeE Short Assay Protocol (SAP) Combo Kit (BioRad Laboratories Inc., Hercules, CA, USA). Positive RPLNs were confirmed by IHC at the Colorado State University Veterinary Diagnostic Laboratory) ...
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bioRxiv - Microbiology 2020Quote: ... IL-22 and TNF-α was analyzed by using quantitative RT-PCR (qRT-PCR) according to the Biorad iScript™ One-Step RT-PCR Kit in a CFX96 apparatus (Bio-Rad, Hercules, CA) with the following primers ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was reverse transcribed into cDNA via iScriptTM Reverse Transcription Supermix and real-time quantitative PCR was performed with SsoAdvancedTM Universal SYBR® Green Supermix in a CFX96 Touch™ thermocycler (all kits and devices from Bio-Rad Laboratories) using 10 ng of cDNA as input ...
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bioRxiv - Biochemistry 2019Quote: ... The microsomal fraction was aliquoted and stored at −80 °C after protein quantification with the Quick Start Bradford Protein Assay Kit (Bio-Rad Laboratories, Munich, Germany).
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bioRxiv - Physiology 2019Quote: ... Defined amounts of mRNAs at 10-fold dilutions were amplified via real-time PCR using iTaq™ Universal SYBR® Green One-Step kit (Bio-Rad, Italy) using the following RT-PCR conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... 500 ng of RNA was then used to generate first strand complimentary DNA (cDNA) using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA). The cDNA was diluted 1:10 before usage and amplified on a LightCycler 480 (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μg of total RNA was reverse transcribed with iScript cDNA Synthesis Kit and amplified by real-time quantitative PCR with IQ-SYBR Green supermix (Bio-Rad, Hercules, CA, USA). ΔΔCt method was used for relative mRNA quantization by applying the equation 2-ΔΔCt.
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bioRxiv - Genetics 2020Quote: ... and 200-500 nanograms of total RNA was programmed for cDNA synthesis using an iScript™ cDNA Synthesis Kit (#1708890 Bio-Rad; Hercules, CA). Universal SYBR Green Supermix (Bio-Rad)-based qRT-PCR was carried out in a CFX386 Touch™Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was diluted 1:30-1:50 in water and qPCR was performed using the iTaq Universal SYBR Green kit (Bio-Rad cat. no. 1725124). Each gene-specific primer and sample mix was run in triple replicates ...
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bioRxiv - Bioengineering 2021Quote: ... at the same concentration and a volume corresponding to 500 ng was added to iScript™ cDNA synthesis kit reagents (Bio-Rad cat# 1708890) for cDNA synthesis ...
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bioRxiv - Biophysics 2021Quote: ... Real-time quantitative PCR was performed with SsoAdvancedTM Universal SYBR® Green Supermix in a CFX96 Touch™ thermocycler (all kits and devices from Bio-Rad Laboratories) using 10 ng of cDNA/ reaction ...
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bioRxiv - Genetics 2021Quote: ... followed by purification with RNeasy minicolumns (Qiagen, μg total RNA was reverse-transcribed using an iScript cDNA synthesis kit (Bio-Rad, Des Plaines, IL). Assay of RNA via quantitative PCR (qPCR ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories, Hercules, CA) and the Taqman primers Cftr (Mm00445197_m1) ...
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bioRxiv - Immunology 2020Quote: ... and gene expression was examined with a Bio-Rad iCycler Optical System using iQ™ SYBR green real-time PCR kit (Bio-Rad Laboratories, Inc.). The data were normalized to 18S ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... Chemiluminescence signals were visualized using the enhanced chemiluminescence kit (ECL; #32106; Thermo Fischer) and a Chemidoc XRS+ scanner system (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2022Quote: ... cDNA was generated from 200 ng of total RNA purified from high and low heme midguts (using a Bio-Rad iScript cDNA synthesis kit) by priming the reaction with random hexamers to ensure the capture of Sgm derived transcripts ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was performed using a SYBR Green PCR Master Mix kit on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). The reaction system (10 μL ...
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bioRxiv - Neuroscience 2023Quote: ... frozen in liquid nitrogen and stored at −80°C until the RNA isolation with Aurum Total RNA Mini Kit (Bio-Rad, Hercules, CA, USA), as described (Makarava et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... cell viability was determined using the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) by measuring the absorbance at 450 nm using an iMarkTM microplate absorbance reader (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2024Quote: ... The mixture was subjected to RT-qPCR analysis using the ChamQ Universal SYBR qPCR master mix kit (Vazyme) in a CFX96™ Real-Time System (BioRad, Hercules, CA, USA). RNA from three biological replicates were analyzed and four technical replicates were performed ...
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bioRxiv - Neuroscience 2024Quote: ... Up to 1000 ng of RNA was used to synthesise cDNA using the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad, USA, #1725035) following the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were physically disrupted in liquid nitrogen before adding lysis buffer from the Aurum Total RNA Mini Kit (catalog #732-6820, Bio-Rad, Hercules, CA, U.S.A.). Further homogenization was conducted using a TissueRuptor II (catalog #9002755 ...