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Citations for Bio-Rad :
5251 - 5300 of 5608 citations for Mouse Leucine Rich Pentatricopeptide Repeat Containing LRPPRC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from PMVECs and Rat lung tissue and subjected to cDNA preparation by the reverse transcription reaction using an iScript cDNA Synthesis Kit (#17088890, Bio-Rad). qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882 ...
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bioRxiv - Plant Biology 2024Quote: ... 10 μL first-strand cDNA was synthesized using 500 ng RNA with iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, USA) following the manufactureŕs instructions ...
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bioRxiv - Genetics 2024Quote: 500 ng of RNA was converted to cDNA using the iScript cDNA synthesis kit (#1708891, Bio-Rad, Hercules, CA, United States) containing random hexamers according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... For western blotting the proteins were transferred to polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo RTA Mini PVDF Transfer Kit (Bio-Rad) and the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotin signal was detected and quantified by chemiluminescence according to standard LightShift Chemiluminescent RNA EMSA Kit protocol using a ChemiDoc™ Touch Imaging System (BioRad).
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bioRxiv - Genetics 2024Quote: ... and cDNA was generated from 0.5 micrograms of purified RNA from each sample using an iScript cDNA Synthesis Kit (Bio-Rad #1708890). qPCR was conducted using iTaq Universal SYBR Green Supermix (Bio-Rad #1725151) ...
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bioRxiv - Biophysics 2024Quote: ... A luminol-based chemiluminescence assay kit (Chemi-Lumi One L, Nacalai tesque) and chemiluminescence detection system (ChemiDoc Touch MP imaging system, Bio-Rad) were used to detect chemiluminescence signals.
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Dynamic regions allosterically connect the USP14 active site with the proteasome interaction surfacebioRxiv - Biophysics 2024Quote: ... and the units were converted to molar ellipticity based on protein concentration measured in the sample by the DC protein assay kit (Bio-Rad) using WT USP14 as standard ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from Rat lung tissue and subjected to cDNA preparation by the reverse transcription reaction using an iScript cDNA Synthesis Kit (#17088890, Bio-Rad). qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882 ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from cells using GeneJet RNA Purification kit (Thermo Fischer) and cDNA was synthesized using iScript Reverse Transcription Supermix (Bio-Rad), as previously described (27) ...
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bioRxiv - Microbiology 2024Quote: ... the blots were washed four times again by PBST and visualized using an Omni-ECL Femto Light Chemiluminescence Kit (EpiZyme, SQ201) through a ChemiDoc MP Imaging System (Bio-Rad). To examine the S glycoprotein packaging efficiency ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then blotted onto polyvinylidene difluoride (PVDF) membranes using Trans-Blot Turbo™ Transfer Starter System and Midi PVDF kit (Both are from Biorad). After transfer ...
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bioRxiv - Plant Biology 2024Quote: ... graminicola genomic DNA was quantified by quantitative PCR using Biozym Blue S’Green qPCR Kit (Biozym Scientific, Germany) in a CFX RT-PCR detection system (Bio-Rad, USA) and specific primers for the histone 3 (CgH3 ...
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bioRxiv - Plant Biology 2024Quote: ... higginsianum genomic DNA quantitation was performed by quantitative PCR using Biozym Blue S’Green qPCR Kit (Biozym Scientific, Germany) in a CFX RT-PCR detection system (Bio-Rad, USA) and specific primers for ChTrpC gene (Supplementary Table S1) ...
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bioRxiv - Plant Biology 2024Quote: ... The rRNA depleted was used immediately to prepare the metatranscriptome libraries using the SEQuoia complete RNAseq kit (Bio-Rad cat# 17005726) following the manufacturer’s procedures without any modifications ...
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bioRxiv - Neuroscience 2024Quote: ... the gel was transferred to a LF PVDF membrane using the Trans-Blot Turbo RTA Midi 0.45 µm LF PVDF Transfer Kit (BIO-RAD, 1704275). After blocking in 5% skim milk powder (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were heat at 95°C for 10 min and then separated by SDS-PAGE electrophoresis on 10% acrylamide gels (FastCast™ Acrylamide kit, BioRad) at 110V for 60 min ...
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bioRxiv - Genetics 2024Quote: ... RNA concentrations were measured with the Little Lunatic (Unchained Labs) and cDNA was synthesized using the iScript cDNA synthesis kit (Bio-rad). Primers for skeletal marker genes were designed using NCBI Primer-BLAST (Supplementary Table 3 ...
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bioRxiv - Immunology 2024Quote: ... The following cytokines and chemokines were measured using a Bioplex Express Kit according to the manufacturer’s directions (Biorad, Hercules, CA, USA): GM-CSF ...
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bioRxiv - Microbiology 2024Quote: ... A total of 500 ng of resulting total RNA was used to synthesize cDNA via random priming utilizing the iScript™ cDNA Synthesis Kit (Biorad). Real-time qPCR was performed on QuantStudio 6 Pro (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: RNA extracts were reverse transcribed into cDNA using an iScript™ Select cDNA Synthesis Kit (Bio-Rad, Marnes-la-Coquette, France) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: We carried out reverse transcription with RNA prepared for transcriptome analysis using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). The cDNA product was diluted 10-fold with water and we used 1Lμl per technical replicate for quantitative PCR analysis ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative RT-PCRs (qRT-PCRs) were carried out in triplicate with three technical replicates with the SsoAdvanced Universal SYBR Green kit (Bio-Rad) on a CFX384 Touch Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Immunology 2024Quote: ... from colonic samples of DR3.IL17A-/- (n = 5) and DR3 mice (n = 5) were reverse transcribed to cDNA using the High-Capacity iScript cDNA synthesis kit (Bio-Rad). qPCR reactions were then carried out in triplicate using 50 ng of cDNA and the Applied Biosystems Power SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Plant Biology 2024Quote: ... 5 μl supernatant was loaded onto 10% SDS polyacrylamide gels and the proteins were transferred onto PVDF membranes using the Trans-Blot Turbo RTA Transfer Kit (1704275, Bio-rad). Membranes were blocked in PBS-T (tablets 524650-1EA ...
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bioRxiv - Developmental Biology 2024Quote: 100 ng of enriched small RNA fraction was used for generated cDNA following iScript Select cDNA Synthesis Kit (#708896, Bio-Rad) following manufacture’s protocol and the specific primer 5′-GCAGGTCCAGTTTTTTTTTTTTTTTCTACCCC-3′ (Supp ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA for measuring primary-miR430 was generated using 200 ng of total purified RNA following iScript cDNA synthesis kit (#1708890, Bio-Rad) manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... One microgram of total RNA was used for a reverse transcriptase reaction using iScript cDNA Synthesis Kit (Catalog #170-8891; Bio-Rad). RT-qPCR analysis was conducted using iQ SYBRGreen Supermix (Bio-Rad ...
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bioRxiv - Synthetic Biology 2021Quote: ... radiodurans genomic DNA was isolated in agar plugs using the Bio-Rad CHEF Genomic DNA Plug Kit (Bio-Rad, CAT#170-3592) with an adapted protocol44 ...
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bioRxiv - Cancer Biology 2021Quote: ... The signal was detected by a chemiluminescent western blot detection kit (Thermofischer) and the blots were developed and visualized under a ChemiDoc system (Amersham, Bio-Rad, USA). The primary antibodies used were Twist2 (GeneTex ...
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bioRxiv - Genetics 2021Quote: ... assessment of protein concentration using Pierce™ BCA Protein Assay Kit (Thermo cat#23225) and addition of 4x Laemmli Sample buffer (Bio-Rad). 40ng of each sample was loaded onto Mini-PROTEAN GTX Precast Protein Gel (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 ng RNA was used for cDNA synthesis for each sample using the iScript cDNA Synthesis kit (Bio-Rad, Cat. No. 1708890). A CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ddPCR was performed on a Bio-Rad QX200 Droplet Digital PCR System using ddPCR Supermix for Probes (No dUTP) kit (Bio-Rad, 1863024) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... the total protein concentration was determined by the Bradford method using the Bio-Rad Protein Assay Kit II (Bio-Rad, 5000 002) and serial dilutions of bovine serum albumin as a calibration standard (BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were detected using the EZ-ECL Chemiluminescence Detection Kit for HRP (Biological Industries) and ChemiDoc XRS+ Gel Documentation System (Bio-Rad Laboratories). Images were taken using the ImageLab™ software (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 1 μg of RNA was subjected to retrotranscription using iScript™cDNA Synthesis Kit (Bio-Rad, Cat. No. 1708890), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 400 ng of complementary DNA (cDNA) was synthesized using the reverse transcriptase iScript complementary DNA synthesis kit (Bio-Rad, Hercules, CA; # 1708891), then diluted to a concentration of 2 ng/μL ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: 250 ng of total RNA of each sample was reversely transcribed and amplified using the TargetAmp-Nano Labeling Kit for Illumina Expression BeadChips (Biozym Scientific GmbH, Hessisch Oldendorf, Germany) on a Biorad MJ Thermal Cycler (Biorad, Munich, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... CLN5 transcripts were measured from the control and CLN5i neurons RNA by quantitative polymerase chain reaction[13] using an iTaq Universal Probes one-step kit (Bio-Rad, 1725141) on a Roche 96-well thermocycler ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleaned samples were solubilized in the previous buffer described above and then quantified with the RCDC Protein Assay kit (Biorad, Hercules, CA). Ten μg of protein from each sample were digested in-solution using both Lys-C and Trypsin ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific) and a T100™ thermal cycler (Bio-Rad). The expression of selected genes was determined by RT-PCR using the iQ Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using the Bio-Rad CFX Manager software ...
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bioRxiv - Molecular Biology 2020Quote: ... Isolated RNA (50 ng) was subjected to qRT-PCR analysis using iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad) in the 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2019Quote: ... First strand synthesis and real time PCR were performed using iScript kit and iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA) on Light Cycler 480 (Roche ...
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bioRxiv - Genomics 2019Quote: Mammalian cells were embedded in gel plugs and High Molecular Weight DNA was purified as described in a commercial large DNA purification kit (BioRad #170-3592). Plugs were incubated with lysis buffer and proteinase K for four hours at 50°C ...
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bioRxiv - Immunology 2019Quote: ... One microgram of RNA was used to synthesize first strand cDNA using the iScript cDNA synthesis kit from Bio-Rad (Hercules, CA). The primers used for qPCR were synthesized by IDT (Coralville ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary anti-mouse antibody (Amersham; NA931S) and anti-rabbit antibody (Amersham; NA934VS) at 1:10,000 and Clarity western ECL substrate detection kit (Bio-Rad; 170-5060) were used to detect the signals ...
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bioRxiv - Immunology 2019Quote: ... and 1 µg of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-rad, cat. #: 1708890). cDNA was diluted to 20 ng/µl and 2.5 µl/reaction was used as input for the qPCR reaction ...
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bioRxiv - Cancer Biology 2019Quote: One microgram of total RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Inc., Hercules, CA). The PCR reactions were performed with 0.2 µl of cDNA template in 25 µl of reaction mixture containing 12.5µl of iQ SYBR Green Supermix (Bio-Rad Laboratories ...