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Citations for Bio-Rad :
5351 - 5400 of 5608 citations for Mouse Leucine Rich Pentatricopeptide Repeat Containing LRPPRC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and ampR was measured by quantitative Reverse Transcriptase PCR (qRT-PCR) using the iTaq Universal SYBR Green One-Step kit (Biorad, Hercules, CA) and a CFX96 Touch Real-Time PCR detection system (Bio- Rad ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the membrane was washed three times with TBST and detection was performed with the Clarity™ Max ECL kit for pulldown experiments (Bio-Rad) and Clarity™ ECL kit for all other experiments (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... This step was repeated two times and the protein concentration was determined by the DC Protein Assay Kit (Bio-Rad, Munich, Germany) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... and cDNA was synthesized from 500 ng of RNA using the iScript kit according to the manufacturer’s protocol (Bio-Rad, 170-8891).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScript™ gDNA Clear cDNA Synthesis Kit (#1725035, Biorad, Mississauga, ON, Canada). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Biorad ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was then generated using the Superscript™ III Reverse Transcriptase kit and pre-amplified with a mixture of the tested TaqMan® probes (Table S1) using the SsoAdvanced™ PreAmp Supermix (Biorad). Differential levels of cDNA expression were measured using the droplet digital PCR (ddPCR ...
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bioRxiv - Microbiology 2019Quote: ... and FLAG-cMCF-HA recovered as described above were prepped using the Bio-Rad ReadyPrep 2-D Cleanup Kit (Bio-Rad 1632130). The sample was then loaded onto 11 cm pH 3-10 immobilized pH gradient (IPG ...
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bioRxiv - Molecular Biology 2019Quote: ... reverse transcription (RT) was carried out with 1 µg of total RNA using the iScriptTM cDNA Synthesis Kit (Bio-Rad, Hercules, CA). Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR was performed by using a Universal SYBR® Green kit and the C1000 Touch Thermal Cycler real-time PCR detection system (Bio-Rad). The EF1α (AT5G60390 ...
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bioRxiv - Physiology 2019Quote: ... Protein concentration was determined in triplicate using a commercial colorimetric assay (Bio-Rad Protein Assay kit-II; Bio-Rad, Gladesville, NSW, Australia). Nuclear and cytosolic fraction enrichment was confirmed by blotting the separated fractions against a nuclear (histone H3 ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was prepared from RNA isolated from the cell lysates using a iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA), and qRT-PCR was conducted as described previously (21 ...
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bioRxiv - Cell Biology 2019Quote: ... A total of 100 ng RNA per sample was subjected to Reverse Transcriptase (RT) by using iScriptTM cDNA Synthesis kit (1708890, BioRad, Hercules, CA). For whole liver ...
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bioRxiv - Cell Biology 2021Quote: ... and 10-14h after egg laying and reverse transcribed using oligo-dT primers with a One-Step RT kit (Bio-Rad 1725140). The coding sequence for each gene encoding a gene of interest was amplified from a cDNA template using Phusion High Fidelity DNA polymerase (Thermo Fisher F-530XL) ...
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bioRxiv - Physiology 2020Quote: ... The total RNA was purified from genomic DNA contamination then converted to cDNA using iScriptTM gDNA Clear cDNA synthesis kit according to manufacturer’s instructions (Bio-Rad, CA, USA). Commercially available SYBR® Green Glucose Metabolism Array consisting of 84 genes were used (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Densitometry of the blots was detected using the Enhanced Chemiluminescence Kit HRP (SQ101, EpiZyme, China) in the ChemiDocTM MP Imaging System (Bio-Rad, USA). Information on the primary antibodies used has been listed and provided (Table EV4).
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was exposed using the Enhanced Chemiluminescence Kit HRP (SQ101, EpiZyme, China) in the ChemiDocTM MP Imaging System (Bio-Rad, USA).
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bioRxiv - Plant Biology 2021Quote: The cDNA synthesis was performed using 1 μg of total RNA and iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and 20 μg of proteins per sample were resolved in 10 or 12% gels (TGX™ FastCast™ Acrylamide kit, Bio-Rad) via electrophoresis ...
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bioRxiv - Genomics 2021Quote: ... Reverse transcription (RT) was performed on 1 μg of DNase-treated total RNA using the iScript cDNA synthesis kit (Bio-Rad #1708890), with the same amount of total RNA diluted in water as negative controls for RT-PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... The product from the final elution and whole cell lysate supernatant were subjected to silver staining using Silver Stain Plus Kit (BIO-RAD, # 1610449) to check pull down efficiency and purity ...
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bioRxiv - Immunology 2021Quote: ... 30 μg of protein was mixed with an equal volume of 2×Laemmli buffer supplemented with 5% 2-mercaptoethanol (Catalog no. 1610737) then loaded in 10% polyacrylamide precast gel (TGX™ FastCast™ Acrylamide Starter Kit, 10%, catalog no. 1610172) from BioRad Laboratories (Mississauga ...
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bioRxiv - Molecular Biology 2021Quote: ... and quantitative PCR (qPCR) was performed to quantify mRNA abundance using a SsoFast™ EvaGreen® Supermix kit (Bio-Rad, 172-5204,) on a Bio-Rad CFX96 cycler ...
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bioRxiv - Immunology 2020Quote: ... Multiplexed cytokine measurements on macrophage cell culture supernatants were performed using a custom Bio-Plex Express Human Cytokine kit (Bio-Rad 17004073) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 30 µg of protein was mixed with an equal volume of 2×Laemmli buffer supplemented with 5% 2-mercaptoethanol (Catalog no. 1610737) then loaded in 10% polyacrylamide precast gel (TGX™ FastCast™ Acrylamide Starter Kit, 10%, Catalog no. 1610172) from BioRad Laboratories (Mississauga ...
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bioRxiv - Cell Biology 2021Quote: ... Cytokine secretion assay was performed using ThermoFisher ProcartaPlex Multiplex Custom Made mouse cytokine 5-plex assay kit following manufacturer’s instructions and analyzed with the Bio- Plex 200 Luminex-based multiplex analysis system (Bio-Rad, Hercules, CA). All samples were analyzed using technical duplicates ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Reverse transcription reactions were performed to synthesize cDNA from 200 ng of total RNA using the iScript™ Select cDNA Synthesis Kit (Biorad, CA). Real-time quantitative PCR (qRT-PCR ...
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bioRxiv - Neuroscience 2021Quote: ... An aliquot was taken to measure radioactivity and protein content using a liquid scintillation counter and a DC protein assay kit (Bio-rad, USA), respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... Gels were stained using the Silver Staining Plus Kit and documented on the ChemiDoc XRS+ system (Bio-Rad Laboratories Inc., Hercules, USA) according to the manufacturers protocol.
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bioRxiv - Genomics 2020Quote: ... the membrane was incubated with peroxidase-conjugated goat anti-mouse IgG and proteins were detected using a chemiluminescence detection kit (Super-Signal West Pico, Pierce) and a ChemiDoc™ MP system (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... and transferred to a 0.20μm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad, Marnes-la-Coquette, France). The membranes were blocked for 30□min in 5% low-fat milk in TBS-0.1% Tween 20 at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The immunoblotting signals were detected using a CCD camera (Intas Biopharmaceuticals, Ahmedabad, India) after application of enhanced chemiluminescence detection kit (Clarity™ Western ECL Substrate; Bio-Rad, Hercules ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting pellets were resuspended in homogenization buffer and centrifuged again at 9000 g for 10 min at 4°C and protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201). Mitochondrial respiration was measured in Mitochondrial Assay Solution (MAS ...
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bioRxiv - Genetics 2022Quote: ... qRT-PCR analysis were performed with iQ-SYBR Green Supermix kit in an iCycler iQ5 system following the instructions of the manufacturer (Bio-Rad, USA). The relative quantifications of mRNA levels were performed using the cycle threshold (Ct ...
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bioRxiv - Synthetic Biology 2022Quote: ... #FABRK 001-1) and 400 ng of cDNA was synthesized using the iScript™ Select cDNA Synthesis Kit (Bio-Rad Laboratories, #1708897) on a Mastercycler® nexus PCR Thermal Cycler (Eppendorf) ...
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bioRxiv - Immunology 2022Quote: ... Oligo-dT first strand cDNA synthesis was performed from 5 μg total RNA with iScript Advance cDNA Synthesis Kit for RT-qPCR (Bio-Rad; #1725038) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was then washed in 6x 5 minutes of TBST prior to being developed with Clarity™ Western ECL Substrate kit (Bio-Rad). To examine actin levels as a loading control blot was stripped using Restore™ WesternBlot Stripping Buffer (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Germany) and 1 μg of total mRNA was translated to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) according to the instructions.
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bioRxiv - Neuroscience 2022Quote: ... 500 ng of total RNA was used in a 20 µL reaction volume with the Bio-Rad iScript cDNA synthesis kit (Bio-Rad, #1708890) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein measurements were performed using a BCA kit (Pierce) and 150 µg was loaded on a 10-20 % tris/tricine SDS-page gels (Bio-Rad Laboratories) and blotted onto 0,2 µm nitrocellulose membrane (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2022Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using gDNA of purified viruses was performed in parallel of each experiment ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription of the RNA was performed to synthesize cDNA following the standard manufacturer’s instructions of the iScript Reverse Transcription Supermix for RT-qPCR kit (Bio-Rad PN 1708841). cDNA was submitted to the University of North Carolina Chapel Hill Advanced Analytics Core (UNCAAC ...
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bioRxiv - Neuroscience 2024Quote: ... The signals were detected using an enhanced chemiluminescent (ECL) plus reagent kit (Share-Bio, Cat. SB-WB012L, China) and viewed with a ChemiDoc MP Imaging System (Bio-Rad Laboratories).
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bioRxiv - Zoology 2024Quote: ... and 100 ng of RNA were used for cDNA synthesis with the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories, USA), resulting in a final concentration of 5 ng/μl for each sample.
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bioRxiv - Microbiology 2023Quote: ... the fluorescence derived from the incorporation of SybrGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoAdvanced Universal SYBR Green Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
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bioRxiv - Bioengineering 2024Quote: Four samples of total RNA were collected for each treatment condition using the Aurum Total RNA Mini Kit (Bio-Rad, Hercules, CA). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein pellet was resuspended in UTC buffer (8 M urea, 2 M thiourea, 4% CHAPS) and quantified using RC DC™ Protein Assay Kit (Bio-Rad). Sample with 80 µg proteins resuspended in rehydration buffer (8 M urea ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of RNA was taken to synthesize complementary DNA (cDNA) conversion reactions were done using an iScript™ Advanced cDNA Synthesis Kit (catalog no. 18080085, Biorad, USA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories) and the TaqMan primers/probe mixtures (all from Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: cDNA was synthesized by reverse transcription from 500 ng of total RNA according to manufacturer’s instructions (iScript™ cDNA Synthesis Kit, Bio-Rad Laboratories). Real-time PCR reactions using primers hybridizing with ERG25 or ACT1 transcripts were performed in an CFX96TM Real-Time System C100TM Thermal Cycler (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using the gDNA of purified viruses was performed in parallel with each experiment ...