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Citations for Bio-Rad :
5101 - 5150 of 5608 citations for Mouse Leucine Rich Pentatricopeptide Repeat Containing LRPPRC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... were detected by staining membranes with anti-mouse IFNγ biotin (1mg/mL) (R46A2) followed by streptavidin-Alkaline Phosphatase (1mg/mL) and development with AP conjugate substrate kit (BioRad, UK).
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bioRxiv - Immunology 2020Quote: ... the signals were detected using an enhanced chemiluminescence detection kit (Super Signal West Pico Plus, 34580, ThermoFischer Scientific) and membranes imaged with ChemiDoc Imaging System (Bio-Rad). The same anti-S1 and anti-S2 antibodies as well secondary antibodies were also used to detect the Spike protein on EVs by ELISA (see protocol below).
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bioRxiv - Molecular Biology 2020Quote: 500ng of genomic RNA were used for reverse transcription into cDNA using the iScript cDNA synthesis kit (Bio-Rad, #170-8891). cDNA was diluted 10X and 2uL was added for each qPCR reaction (iQ™ SYBR Green Supermix ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence substrate was then applied to the blot according to the manufacturer’s instructions (Bio-Rad Clarity™ Western ECL Substrate kit). The chemiluminescent signals were recorded using a Syngene G:BOX (Synoptics ...
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bioRxiv - Cancer Biology 2019Quote: Plasma activities of ALT and AST were assessed with the ALT and AST Reagent Kit (Biosystems Reagents) using a Benchmark Plus microplate spectrophotometer (Bio-Rad). Plasma concentration of non-sulfated bile acids were measured with the Bile Acid Assay Kit (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... ATAC Tagmentation Buffer and ATAC Tagmentation Enzyme (ATAC Tagmentation Buffer and Enzyme are both included in the SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad)) ...
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bioRxiv - Genomics 2021Quote: For a detailed description of tagmentation protocols and buffer formulations refer to the SureCell ATAC-Seq Library Prep Kit User Guide (17004620, Bio-Rad). Harvested cells and tagmentation related buffers were chilled on ice ...
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bioRxiv - Genomics 2021Quote: ... One µg of RNA was then used to synthesize cDNA using the Bio-RAD iScript cDNA Synthesis Kit (Bio-RAD, 1708840). qPCR reactions for targeted genes were performed with the Luminaris HiGreen qPCR Master Mix (Thermo Scientific ...
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bioRxiv - Genomics 2021Quote: The nuclei solution were adjusted to 43°C for 3 min and melted 2% agarose from CHEF Genomic DNA Plug Kits (Bio-Rad) was added to reach a 0.82% agarose plug concentration ...
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bioRxiv - Microbiology 2021Quote: ... total 600 ng of RNA was used for cDNA synthesis by using Random hexamer oligonucleotide primer (iScript Select cDNA Synthesis Kit, BioRad, USA). Gene specific primers (Table S2 ...
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bioRxiv - Genomics 2020Quote: ... The concentrations of cytokines in plasma were measured using the Bio-Plex Pro™ Human Cytokine 17-plex Assay kit immunoassay run on the Bio-Plex 200 (BioRad), with reference to an 8 point standard curve.
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bioRxiv - Microbiology 2021Quote: ... First-strand cDNAs were synthesized from 500 ng DNase-treated total RNA by RT with the iScript cDNA synthesis kit (Bio-Rad) in a total volume of 20 µL according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Serum was also collected for cytokine analyses which were performed by the University of Pennsylvania’s Human Immunology Core using a Non-Human Primate Cytokine Panel kit (MilliporeSigma, Cat# PCYTMG-40K-PX23) on a Bio-Plex 200 instrument (Bio-Rad) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane by semi-dry transfer using the Trans-Blot turbo RTA Mini PVDF transfer kit (Bio-Rad) and the Trans-Blot turbo system ...
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bioRxiv - Physiology 2021Quote: ... was performed with the Bio-Rad iQ TM SYBR Green Supermix kit and data were analyzed with the CFX384 system (Bio-Rad). mRNA quantitation was done by normalizing the amount of RNA detected for the gene of interest ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time quantitative reverse-transcription polymerase chain reaction (RT-qPCR) was performed using the iTaq Univer SYBR Green 1-Step Kit (Bio-Rad) on a StepOnePlus Real-time PCR system (Applied BioSystems ...
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bioRxiv - Developmental Biology 2021Quote: ... eluted total RNA in 10 μl of Elution buffer and reverse transcribed RNA in 20 μl using the iScript cDNA synthesis kit (Bio-Rad). We incubated the samples for 5 min at 25°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA (1μg) of each postnatal stage was used for cDNA synthesis using a reverse transcription kit (iscript, Bio-Rad, USA). Real-time PCR amplification was performed using iTaq™ Universal SYBR® Green Supermix (BioRad ...
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bioRxiv - Biochemistry 2021Quote: ... The antigen-antibody complexes were visualized using appropriate HRP conjugated secondary antibodies and enhanced chemiluminescence kit (ECL; Amersham or Bio-Rad) and normalization was reported to β-actin ...
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bioRxiv - Physiology 2021Quote: ... 5 ul of each supernatant sample was used to quantify the total amount of protein with a BCA Protein Assay Kit whereas the rest was diluted in 4x Laemmli sample buffer (Bio-Rad) with 2-Mercaptoethanol (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... markers was analyzed by qPCR as previously described (Yanai et al., 2013; Yanai et al., 2015) using an iScript cDNA synthesis kit (Bio-Rad) and TaqMan Primer/probe sets for each gene ...
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bioRxiv - Immunology 2020Quote: ... with 18S (Hs99999901_s1) as housekeeping control were carried out on RNA reverse transcribed to cDNA with an iScript cDNA synthesis kit (Bio-Rad), using Taqman chemistry and gene-specific probes purchased from Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: Total RNAs were extracted from cell-sorter purified cells using NucleoSpin RNA kit with a DNase treatment (Macherey Nagel) and reverse transcribed with iScript™ Reverse Transcriptase mix (Biorad) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Reverse transcription and real-time PCR (qPCR) reactions were carried out using iScript cDNA synthesis kit and iQ SYBR Green Supermix (Bio-Rad). qPCR was performed on a Bio-Rad CFX384 Touch™ Real-Time PCR Detection System using the following human primers (Forward and Reverse ...
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bioRxiv - Immunology 2021Quote: Plasma from each patient sample was analyzed using the Bio-Plex Pro™ Human Cytokine 17-plex Assay kit (Bio-Rad). Cytokine expression was measured using the Luminex xMAP® (Luminex Corporation ...
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bioRxiv - Molecular Biology 2020Quote: ... The quality and concentration of the RNA was assessed and first strand cDNA synthesised from 1 ug using the iScript Select cDNA Synthesis Kit (Bio-Rad) and a 1:1 mixture of Oligo (dT ...
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bioRxiv - Microbiology 2021Quote: ... The protein contents of 100-µl cell samples were measured using the Bio-rad Protein Assay kit (Bio-rad, CA, USA). Uptake rates were calculated from retained activity and total added activity and expressed in pmol amino acid µg-1 of protein.
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bioRxiv - Microbiology 2021Quote: RNA copies of SARS-CoV-2 samples were detected by quantitative real-time RT-PCR (RT-qPCR) assays were performed using the iTaq SYBR Green One-Step Kit (Bio-Rad) on the LightCycler 480 system (Roche ...
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bioRxiv - Immunology 2021Quote: ... Sera were collected for cytokines analyses which were performed by the Macau University of Science and Technology using a Non-Human Primate Cytokine Panel kit (MilliporeSigma, Cat# PCYTMG-40K-PX23) on a Bio-Plex 200 instrument (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... According to kit instruction 10 μl reaction system was prepared and q-PCR was operated using CFX96 Real-Time System (BIO-RAD). For data analysis ...
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bioRxiv - Genetics 2020Quote: ... Equal amounts of DNA-free total RNA were converted to cDNA using the iScript™ cDNA synthesis kit (Bio-Rad #1708891). Quantitative PCR (qPCR ...
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bioRxiv - Bioengineering 2020Quote: ... anti-CD63 and anti-CD81 detection antibodies (supplied in the MACSPlex kit, 5 µl each) or anti-decoy receptor antibodies (AlexaFluor647-labelled anti-human TNFR1, Bio-Rad, cat #MCA1340A647 ...
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bioRxiv - Microbiology 2020Quote: ... the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad). The results were analyzed using Bio-Rad CFX Manager software ...
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bioRxiv - Neuroscience 2021Quote: ... adult testes or hippocampus or cell pellets were homogenized in RIPA buffer and the protein concentration was determined using a Bradford Assay kit (Bio-Rad). For blot analysis ...
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bioRxiv - Neuroscience 2022Quote: RNA isolation for all tissues were carried out using the Aurum Total RNA isolation kit (BioRad #732-6820; Hercules, CA, USA). Samples were reverse-transcribed to cDNA using qScript cDNA Supermix (Quantabio #101414-106 ...
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bioRxiv - Immunology 2022Quote: ... Separated proteins were transferred to a PVDF membrane using the Trans-Blot Turbo RTA Transfer Kit (Bio-Rad Laboratories, Feldkirchen, Germany) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized from the purified RNA by both random and oligo(dT) priming using the iScript cDNA synthesis kit (Bio-rad). RNA levels were measured by the SYBR Green relative quantitation method using either a 7300 or Viia 7 RT-PCR machine (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 μg of RNA was used to synthesize cDNA with Bio-RAD iScript cDNA Synthesis Kit (170-8891, Bio-Rad). qRT-PCR was performed using Taqman universal PCR mix and predesigned gene expression assays with best coverage from Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA concentration was measured using a NanoDrop spectrophotometer and 1 µg of RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad). Primers sequences were obtained from Harvard primerbank (sequences are listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 3-4 μg of total RNA was reverse transcribed using the iScript advanced cDNA synthesis kit (Bio-Rad). Quantitative PCR of target genes (Table 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed to cDNA using the iScript™ gDNA Clear cDNA synthesis kit (Bio-RAD). qPCR was performed with Applied Biosystems Fast SYBR Green master mix on an Applied Biosystems QuantStudio 6 Flex system ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was carried out for 1 hour at 42°C using the iSCRIPT cDNA synthesis kit (Bio-Rad, Cat #1708841BUN) and 300 ng - 1 μg of RNA per reaction ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysates were sonicated with a microtip for 5 sec at 20% amplitude and quantitated in DC Protein Assay kit II (BioRad, 5000112) against a BSA standard curve ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... BP176-100) and run on a 10% SDS-PAGE using the TGX Stain-free FastCast Acrylamide Kit (Bio-Rad Cat# 1610183) and the Mini-PROTEAN system (Bio-Rad Cat# 1658006) ...
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bioRxiv - Genetics 2022Quote: Approximately 500 ng of total RNA was used to synthesize cDNA using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The HRM analysis was done according to the protocol provided by the Precision Melt Supermix Kit (Bio-Rad; Catalog #172-5112). The following oligonucleotides were used for the HRM analysis ...
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription of 500ng of total RNA was performed using the iScript cDNA synthesis kit (Bio-Rad Laboratories, Inc, California, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... methanol in 1X TBE buffer with 10% (v/v) acetic acid) and RNA was visualised using the Silver Stain Plus Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 1µg of each total RNA sample was used to synthesize cDNA using the i-Script cDNA synthesis kit (Bio-Rad, USA). The real-time PCR was performed using a 20µl reaction volume on 96-well plates by using i-Taq Syber Green (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: Luminex beads used for the serological and purified antibody binding assays were prepared by covalent coupling of SARS-CoV-2 proteins with MagPlex beads using the manufacturer’s protocol with a Bio-Plex Amine Coupling Kit (Bio-Rad, France). Each of the SARS-CoV-2 Spike proteins expressed with different mutations were coupled with different colored MagPlex beads so that tests could be performed with a single protein bead per well or in a multiplexed Luminex binding assay ...