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Citations for Bio-Rad :
5451 - 5500 of 5608 citations for Mouse Leucine Rich Pentatricopeptide Repeat Containing LRPPRC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred into clean tubes and the protein concentration was determined using the Bradford protein assay kit (Bio-Rad Laboratories, USA). The protein extracts (20 μg ...
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bioRxiv - Biochemistry 2021Quote: Reverse transcription (RT) was carried out with 500 ng of total RNA using the iScript™ cDNA Synthesis kit (Bio-Rad, Hercules, CA). qRT–PCR was carried out with the iQ™SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... and up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad, 172-5038). Quantitative real time-PCR was performed using Go-Taq qPCR Master Mix (Promega ...
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bioRxiv - Genetics 2022Quote: ... and cDNA was synthesized by with iScript™ cDNA synthesis kit according to the directions of the manufacturer (Bio-Rad, Hercules, CA, USA). Quantitative PCR was performed using Power SYBR™ Green PCR Master Mix kit on a StepOne Plus real time PCR system (Applied Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... One µg of DNase treated RNA was used to synthesize cDNA using iScriptTM Select cDNA synthesis kit (catalog#170-8896, Bio-Rad, Hercules, CA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Quality and quantity of retrieved RNA was assessed using the Experion automated electrophoresis system and a RNA StdSens Analysis kit (Bio-Rad Ltd., UK).
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bioRxiv - Microbiology 2020Quote: ... was used for quantification and all PCR reactions were prepared using the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad Cat# 1864022). Primers used were previously described in (25) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Reverse transcription was performed with Onescript® cDNA synthesis kit (Abm) and qRT-PCR was performed using SsoAdvanced Universal SYBR® Green Supermix (Bio-Rad) and a CFX96™ Real-Time System device (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScript™ gDNA Clear cDNA Synthesis Kit (#1725035, Bio-Rad, Mississauga, ON, Canada). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... and 200 ng DNA were used to analyze KRAS codon 12/13 and 61 mutations with ddPCR KRAS G12/G13 and KRAS G61 Screening Kits and QuantaSoft Analysis Pro software (Bio-Rad, Hercules, CA) as described elsewhere63 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by electrophoresis on a 10% SDS polyacrylamide gel and visualised with Silver Stain Plus Kit (Bio-Rad Laboratories AB, Sweden) according to manufacturer’s modified silver stain protocol.
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were normalized for protein concentration using a BCA assay kit (Thermo 23225) and mixed with Laemmli buffer (Bio-Rad 161-0737). 50 µg of the protein mix were run through a 4-20% Criterion TGX gel (Bio-Rad 5671093 ...
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bioRxiv - Plant Biology 2021Quote: ... The protein concentration of each of the five biological replicates was estimated using a Bradford protein assay kit (Bio-Rad Laboratories, Hercules, USA).
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bioRxiv - Microbiology 2020Quote: ... the outer membrane control proteins OmpA (α-OmpA was kindly provided by Prasadaro Nemani (45)) or MOMP (α-MOMP, MONOFLUO™ Legionella pneumophila IFA Test Kit, BioRad), the inner membrane control protein LepB (antibody kindly provided by Gunnar von Heinje (46) ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentration in supernatant will be determined by Bradford assay using the Bio-Rad Protein Assay kit (Bio-Rad, Hercules, CA, USA). Equal amounts of total protein are separated on 8∼12% SDS–PAGE gels ...
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bioRxiv - Cell Biology 2021Quote: ... The total RNA (1 μg) from each sample was reverse transcribed into cDNA using the iScript cDNA select Synthesis Kit (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Thirty nanograms of cDNA per reaction were analyzed for semi-qPCR using the SsoAdvanced™ Universal SYBR® Green Supermix Kit (Bio-Rad) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatant was used for various assays and the protein content of the homogenate was measured using DC protein assay kit (Bio-Rad, Hercules, CA). Lactase activity was assessed using a Lactase Assay Kit (Cat# E-BC-K131-S ...
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bioRxiv - Immunology 2021Quote: ... First-strand cDNA were synthesized from total RNA using an iScript gDNA Clear cDNA synthesis Kit according to the manufacturer’s instruction (Bio-Rad, Hercules, CA, USA). Real-time PCR was performed using the StepOnePlus Real-time PCR system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... Total AGT concentration in each sample was normalized by total protein concentrations measured using a DC™ protein assay kit (Bio-Rad 5000112).
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bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 µg) from each sample sets (n=3) was taken for cDNA preparation using iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Real-Time qPCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... 30-50µg of protein per lane alongside a prestained molecular weight protein marker was separated on an 8% SDS PAGE gel prepared from SDS-PAGE kit (Beyotime, China) and electrotransferred to immunoblot PVDF membrane (BIO-RAD, CA, USA) for protein blotting ...
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bioRxiv - Neuroscience 2021Quote: ... was used from each sample for One-step reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) using the iTaq™ Universal SYBR® Green One-Step kit (Bio-Rad) in the StepOnePlus™ Real Time PCR system (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2020Quote: ... Results were validated using qPCR experiments where mRNA was reverse transcribed into cDNA using the iScript cDNA synthesis kit (cat #1708891, Bio-Rad, Hercules, CA). SYBR Green based qPCR was performed on a CFX-Connect Real Time PCR Detection System (Bio-Rad ...
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bioRxiv - Genetics 2019Quote: ... We measured total protein concentrations of cell lysates with the BCA protein assay kit (Pierce, Cat#23225) and diluted 40 μg of each protein with XT sample buffer (Bio-Rad, Cat#1610791) and XT reducing agent (Bio-Rad ...
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bioRxiv - Genetics 2020Quote: ... and was quantified by using a QuantifilerR Human DNA Quantification Kit (Applied Biosystem, USA) on a Bio-Rad Real-time PCR System (Bio-Rad Laboratories, USA). The quantification process was performed per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed by using a HiScript II one-step qRT-PCR SYBR green kit (Vazyme) on a CFX96 real-time PCR detection system (Bio-Rad Laboratories, Inc.). The following primers were used ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-rad, cat. no. 1708890) or Superscript III (Invitrogen cat ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 0.2-1 µg of RNA was pre-treated with DNAse and used for cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad, Irvine, CA). Quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were separated at 200V for 45 min and then transferred to a low-fluorescence PVDF membrane at 25V for 30 min using the Trans-Blot Turbo Transfer System and Kit (Bio-Rad, Cat. #1704275). Gels were activated by UV excitation using the Bio-Rad GelDoc XR+ System prior to transfer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Reverse transcription was performed with Onescript® cDNA synthesis kit (Abm, #G236) and qRT-PCR was undertaken using SsoAdvanced Universal SYBR® Green Supermix (Bio-Rad) and a CFX96TM Real-Time System device (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... Both anti-IL-9 and the isotype antibody were purified from the culture supernatant of hybridoma cells using the Proteus protein purification spin kit (Bio-Rad, Hercules, CA). Anti-IL-13 is an IgG1 antibody (55 ...
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bioRxiv - Neuroscience 2022Quote: ... First strand cDNA was prepared from 400 ng of total RNA and the complementary DNA strand was synthesized using iScript cDNA synthesis kit (Bio-Rad, #1708891, USA), according to the manufacturers protocol ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA). qPCR was performed with the SYBR Green I nucleic acid gel stain (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and 5 U/mL aprotinin) and the protein concentrations in the lysates were determined using a BioRad protein assay kit (BioRad Laboratories, Hercules, CA). Protein samples were subjected to SDS-PAGE ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cervicornis sperm samples from 2015 were brought to a final concentration of 3 x 107 cells ml-1 after the addition of Cell Suspension Buffer and 2% agarose using the Bio-Rad CHEF Genomic DNA Plug Kits (Bio-Rad, Hercules, CA). Genomic DNA plugs were processed according to the manufacturer’s protocol and stored at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... one-step reverse transcription droplet digital PCR (RT-ddPCR) assays were performed with the One-step RT-ddPCR Advanced Kit for probes (Bio-Rad Laboratories Ltd.), using primers and probes targeting 2’,5’-oligoadenylate synthetase 1 (OAS1) ...
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bioRxiv - Molecular Biology 2024Quote: The synthesis of cDNA was done from 100 ng RNA in 20 μl total reaction volume using iScript Reverse Transcription Supermix kit (Cat# 1708841, Bio-Rad, Hercules, USA), without any changes from themanufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... A total of 1000 ng of RNA was used for cDNA synthesis with the High-Capacity cDNA Reverse Transcription Kit (Bio-Rad, Hercules, CA). Real-time PCR was performed with iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: Total protein lysates were prepared from the cells followed by protein quantification using the protein DC assay kit (Bio-Rad, Cat # 5000-001) as described previously (28) ...
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bioRxiv - Pathology 2023Quote: Rabies virus N gene was amplified by real-time quantitative PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD). The primers are as follow ...
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bioRxiv - Microbiology 2022Quote: ... and TNF-α was analyzed by using quantitative real-time PCR according to the Biorad iScript One-Step RT-PCR Kit in a CFX96 apparatus (Bio-Rad, Hercules, CA) with the following primers (F/R) ...
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bioRxiv - Cancer Biology 2023Quote: PDEC cytokine secretion was analyzed from cleared PDEC culture supernatants using Bio-Plex Pro Human Cytokine 27-plex assay kit (Bio-Rad, cat. M500KCAF0Y) and Bio-Plex 200 System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... RNA reverse transcription and PCR amplification were performed in a single reaction using the One-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, CA, USA) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration in cell lysates were measured using a RC DC Protein Assay Kit (cat. no. 50000119; Bio-Rad Laboratories, Hercules, CA, USA). Immunoblotting was carried out using standard procedures ...
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bioRxiv - Physiology 2023Quote: One hundred nanograms of total RNA was reverse-transcribed (RT) and amplified using the iScript One-Step RT-PCR kit for probes (Bio-Rad, Hercules, CA). Real-time qPCR was performed with the Bio-Rad CFX96 sequence detection system using predesigned primer/probe sets against CPT1a ...
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bioRxiv - Developmental Biology 2023Quote: Genomic DNA was extracted from a liver of an adult female ferret using the CHEF Mammalian Genomic DNA Plug Kit (BioRad, Cat. No. #1703591). In brief ...
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bioRxiv - Cancer Biology 2023Quote: ... The resultant homogeneous single-cell suspensions were embedded in agarose and transferred into plug molds (Bio-Rad CHEF Mammalian Genomic DNA plug kit). Plugs were allowed to solidify at 4°C and were then incubated with Proteinase K (QIAGEN ...
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bioRxiv - Immunology 2023Quote: ... Equal concentrations of RNA (measured by NanoDrop and 1 µg for the caecum tissue) were reverse transcribed to cDNA using the iScript™ cDNA Synthesis Kit (Bio-Rad, #1708891) and stored at −20°C ...
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bioRxiv - Cell Biology 2022Quote: ... The purified RNA was quantified by NanoDrop and 1 µg of RNA for each sample was used to generate cDNA using the iScript cDNA synthesis kit (Biorad cat. no. 1708890). Real-time PCR was performed with PowerUp SYBR Green Master Mix (Applied Biosystems cat ...