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Citations for Bio-Rad :
5401 - 5450 of 5608 citations for Mouse Leucine Rich Pentatricopeptide Repeat Containing LRPPRC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was conducted using HiScript II One Step qRT-PCR SYBR Green Kit (Q221-01, Vazyme) with gene-specific primer pairs on a CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories). The thermal cycling protocol was as follow ...
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bioRxiv - Bioengineering 2024Quote: RNA was diluted in nuclease-free water and combined with the reagents from the One Step RT-ddPCR Advanced Kit for Probes (Bio-Rad, USA) and eGFP primer/probe set with final primer concentrations of 900 nM and probe concentrations of 250 nM to measure transgene expression ...
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bioRxiv - Molecular Biology 2023Quote: ... where absolute quantification was done by the QX200 Droplet Digital PCR System in combination with the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, USA). The detection limit was calculated to be 1000 copies per reaction ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged 10 minutes at 13,000 x g and the supernatant was quantified using a DC assay kit (Bio-Rad, Hercules, California). 200 µg of total protein were diluted into a total of 1.5 mL Array Buffer and rocked over the pre-blocked array membrane containing various total RTK antibodies overnight at 4°C ...
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bioRxiv - Pathology 2023Quote: ... and the other one was subjected to the western blot assay using the Trans-Blot Turbo RTA Midi 0.45 µm LF PVDF Transfer Kit (Bio-Rad Laboratories, CA). After transferring the total proteins to the PVDF membrane ...
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bioRxiv - Microbiology 2023Quote: ... for real-time PCR analysis of SARS-CoV-2 E genes using the iTaq Universal Probes One-Step RT-PCR Kit (Bio-Rad, USA) and the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Neuroscience 2023Quote: ... Once ready for testing we quantified the amount of mitochondria from each isolation using Bio-Rad Protein Assay Kit (Bio-Rad, #5000001), plated 3µg/50µl/well onto a 96 well plate with all animals having 6-8 technical replicates (101085-004 ...
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bioRxiv - Physiology 2023Quote: ... The cDNA was obtained from equal amounts of total RNA by reverse transcription using an iScriptTM cDNA Synthesis Kit (Bio-Rad, CA). Gene expression of 96 fibrosis-associated genes were evaluated using a PCR array kit (RT² Profiler™ PCR Array Rabbit Fibrosis PANZ-120Z ...
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bioRxiv - Plant Biology 2023Quote: ... The 1st strand cDNA was synthesised from 1 µg of RNA for each sample using iScript™ cDNA Synthesis Kit (Bio-Rad). This cDNA was then used as the template in qRT-PCR reaction mixture after 10 times dilution ...
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bioRxiv - Microbiology 2023Quote: ... modified from previously published primers/probe (44)) was performed using the BioRad iTaq Universal Probes One-Step Kit (BioRad, Cat. No. 1725140). Thermocycling was performed on a BioRad CFX Connect Real-Time PCR Detection System (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... Complementary DNA (cDNA) synthesis was performed using 1 μg of total RNA with the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). The genes encoding for the zonula occludens-1 (Zo-1) ...
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bioRxiv - Biochemistry 2023Quote: ... The determination of casein concentration by the Lowry method was performed using the DC™ protein assay kit purchased from Bio-Rad Laboratories (California ...
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bioRxiv - Cancer Biology 2023Quote: ... Complementary DNA (cDNA) was synthesized with 1 µg of total RNA by using the iScript cDNA Synthesis Kit (Bio-Rad, Cat# 1708891). cDNA was diluted 20 times and real-time quantitative PCR was performed by using iTaq Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Visualization was performed using a horseradish peroxidase sensitive ECL western blotting detection kit (SuperSignal West Pico PLUS Chemiluminescent Substrate, ThermoScientific) and the result was recorded using Chemidoc Imaging System (Bio-Rad Laboratories).
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bioRxiv - Plant Biology 2023Quote: ... Membranes were developed using the SuperSignal West Pico Chemiluminescent Substrate kit (Fisher #PI34578) and visualized on a ChemiDoc™ Touch Gel Imaging System (BioRad #1708370).
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bioRxiv - Bioengineering 2023Quote: ... We then added 80 μL of X1 streptavidin phycoerythrin SA-PE (Bio-Plex Pro Reagent Kit III #171304090M, BioRad, Hercules, CA, USA) and incubated it for another 20 minutes ...
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bioRxiv - Physiology 2023Quote: ... One hundred nanograms of RNA was used as input to synthesize cDNA using the iScript cDNA synthesis kit (BioRad, Hercules, CA, USA). cDNA templates were used for quantitative real-time PCR ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 4°C at 20,160 x g for 10 minutes and supernatants were collected for protein measurement using the DC Protein Assay Kit (Bio-Rad, 5000111). Equal amounts of protein were loaded to run the gels (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified with the RC DC Protein assay Kit II (cat. No. 5000122; Bio-Rad Labortaories Inc, California, USA). After protein concentration assessment ...
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bioRxiv - Genetics 2023Quote: ... Whole cell extracts were sonicated and cleared by centrifugation and protein concentration estimated using the Bio-Rad DC protein assay kit (Bio-Rad Laboratories).
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bioRxiv - Bioengineering 2023Quote: ... After RNA isolation the respective complementary DNA (cDNA) was then synthesized using the Bio-Rad iScript cDNA synthesis kit (Bio-Rad, USA) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... First-strand cDNA was synthesized from 200 ng of total RNA in a 20 μl reaction using the iScript™ cDNA Synthesis Kit (Cat#: 1708891, Bio-Rad). A SYBR green real-time PCR kit (Cat# 001752A ...
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bioRxiv - Pathology 2023Quote: ... Two μg of total RNA from each sample were used for cDNA synthesis using the iScript Advanced cDNA Synthesis kit (Bio-Rad, CA).
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bioRxiv - Genetics 2023Quote: ... and luciferase activities were normalized to total protein levels in the WCEs extracts determined using the Bradford assay kit (Bio-Rad Laboratories).
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bioRxiv - Immunology 2023Quote: ... Proteins in the gels were transferred to the PVDF membrane using the Trans-Blot Turbo Kit by the Trans-Blot Turbo transfer system (Bio-Rad, 1704156). Membranes were then incubated in a blocking buffer (Nacalai Tesque ...
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bioRxiv - Immunology 2024Quote: ... In-hospital HIV testing was done on all patients by the clinical teams using point-of care Genie™ HIV1&2 test kits (BioRad, USA).
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bioRxiv - Microbiology 2024Quote: qRT-PCR analysis was performed on three major selected genes and validated as follows: The first cDNA strand was prepared using the iScript™ cDNA synthesis kit (Bio-Rad). qRT-PCR was performed using the SsoFast™ EvaGreen® supermix kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... CD45-APC and Bu1-PerCP-Cy5.5 were generated by conjugation of the antibody with the respective Lynx Rapid conjugation kit (Bio-Rad, Feldkirchen, Germany). Samples were analyzed on a FACS Canto II and FlowJo 10.9 software (BD ...
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bioRxiv - Microbiology 2024Quote: ... The anti-HBs concentrations of the DBS samples were tested using the Monolisa Anti-HBs PLUS kit (Bio-Rad, Hercules, CA, USA) with a 2.00 mIU/mL lower limit of detection (LoD ...
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bioRxiv - Developmental Biology 2024Quote: ... using cDNA generated from 0.1-2 ng total RNA with the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad catalog # 1864021) according to the manufacturer’s protocol with reverse transcription at 42°C and annealing/extension at 60°C.
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized using the iScript™ cDNA Synthesis Kit according to the manufacturer’s instructions (Cat#1708890, Bio-Rad Laboratories, Hercules, CA). Quantitative real-time PCR was conducted using SYBR Green probes and measured using the QuantStudio using the following PCR settings ...
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bioRxiv - Microbiology 2024Quote: ... In-hospital HIV testing was done on all patients by the clinical teams using point-of care Genie™ HIV1&2 test kits (BioRad, USA).
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bioRxiv - Microbiology 2024Quote: ... The anti-HBs concentrations of the DBS samples were tested using the Monolisa Anti-HBs PLUS kit (Bio-Rad, Hercules, CA, USA) with a 2.00 mIU/mL lower limit of detection (LoD ...
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bioRxiv - Plant Biology 2024Quote: ... The transcripts of defense marker genes were quantified by using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD, #1725150) with 40 cycles of 1 s at 95°C and 30 s at 60°C.
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bioRxiv - Developmental Biology 2021Quote: ... Real-Time reactions were performed in triplicates using KAPA SYBR FAST qPCR Kit (2X) on QuantStudio 5 Real-time PCR system (Bio-Rad, CA, USA) following the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was isolated from the cerebellum using the Aurum™ Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad, Hercules, CA, USA). Reverse transcription was performed using the iScript™ cDNA synthesis kit (Bio-Rad) ...
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bioRxiv - Genomics 2019Quote: ... Extracted RNA was then transcribed to first-strand complementary DNA (cDNA) using the iScript Select cDNA synthesis kit (Bio-Rad laboratories, CA, USA) with its proprietary random primer mix ...
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bioRxiv - Immunology 2021Quote: ... DNA contamination in the treated RNA samples was assessed by performing a qPCR cycling with tilapia elongation factor 1α (EF-1α) primers using No-RT master mix (absence of reverse transcriptase enzyme provided in iScript™ Reverse Transcription kit, Bio-Rad, USA). The cDNA synthesis (20 µL reactions ...
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bioRxiv - Synthetic Biology 2019Quote: ... Gels were either silver-stained using SilverQuest™ Silver Staining Kit (Novex) according to the protocol of the manufacturer or Coomassie stained using Bio-Safe™ Coomassie Stain (Bio-Rad Laboratories). For densitometric determination of SDS-PAGE gel bands ...
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bioRxiv - Neuroscience 2019Quote: ... Instructions were followed as per the Bio-Rad Aurum Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad, Hemel Hempsted, UK, Cat# 7326830) and our previously optimised protocol (Pervolaraki et al. ...
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bioRxiv - Microbiology 2019Quote: One hundred microlitres of recombinant ZIKV NS1 antigen (ensuring at an optimal protein concentration range of 0.5-5.0mg/ml) were coupled to 100μg of lyophilized HRPO mix using the LYNX Rapid HPR Conjugation kit (Bio-Rad Laboratories Ltd, Watford, UK) in accordance with the manufacturer’s instructions Once conjugated the product was diluted 1 in 10 in HRP Stabilising Buffer (ClinTech ...
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bioRxiv - Immunology 2019Quote: ... cDNAs were synthesized from 1 μg total RNAs and qPCR were performed by using the iScript™ cDNA Synthesis Kit (BioRad, the Netherlands) and iQ™ SYBR Green Supermix (BioRad ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The blood was first separated from the ethanol through gentle centrifugation and embedded in molten 2% agarose plugs (Bio-Rad DNA plug kit). The solidified plugs were submerged in Lysis Buffer solution (Bionano ...
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bioRxiv - Plant Biology 2021Quote: ... The cDNA synthesis and qRT-PCR was performed as described earlier (Neogy et al., 2019) using iScript cDNA synthesis kit and iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, India). Rice UBQ5-normalized ΔΔCt was used to calculate log2 fold change ...
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bioRxiv - Plant Biology 2020Quote: ... and the quantitative real-time polymerase chain reaction (qRT-PCR) was performed using the iScript™ One-Step RT-PCR Kit (Bio-Rad®), marking with SYBR® Green (Bio-Rad®) ...
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bioRxiv - Microbiology 2020Quote: ... MNV or HAV RNA recovered by the ISO 15216-1 and PGM-MB or HAV antibody methods was quantified using the One-Step dd-RT-PCR Advanced Kit for Probes (Bio-Rad Laboratories, Ltd.) according to manufacturer’s instructions and as described previously (23 ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μg total RNA was reverse transcribed into cDNA in a reaction volume of 20 μl using the iScript™ Advanced cDNA Synthesis Kit (Bio-Rad, 1725038). Real-time PCR reactions were performed on the resulting cDNA using the CFX Connect™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... 500 ng of total RNA were used in cDNA synthesis with iScript™ Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad, USA) while 700 ng of total RNA from the AT2 callus in LD and LL were used as an input ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were transferred to new tubes and protein concentrations were determined using the DC protein assay according to standard protocols provided by the kit manufacturer (Bio-Rad, Hercules, CA). Equal volumes of soluble protein supernatants were transferred to new tubes and subjected to in solution digestion ...
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bioRxiv - Cell Biology 2022Quote: ... The total amount of protein isolated from the weighed tissues was determined by using a protein assay kit (Bio-Rad, Hercules, CA, USA). Hydroxyproline content data are expressed as μg of collagen per mg of total lung protein (μg/mg).