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Citations for Bio-Rad :
5251 - 5277 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... followed by 40 cycles (95°C for 5 seconds, 60°C for 40 seconds) on a CFX96 Real-Time PCR System (Bio-Rad, Hercules, CA, USA). The housekeeping gene GAPDH (PY17X_1330200 ...
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bioRxiv - Neuroscience 2023Quote: ... 40ug of each sample was denatured at 95°C for 5 minutes and loaded in 4–20% Mini-PROTEAN® TGX™ Precast protein gels (Bio-Rad; SA) with a gel lane including a Dual Xtra standard protein ladder (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Then the membrane was incubated with mouse monoclonal antibodies against PAR for 60 min in PBS-T/5% milk followed by incubation with the secondary anti-mouse antibodies conjugated with horseradish peroxidase (Bio-Rad, Hercules, CA, USA) for 60 min in the PBS-T/5% milk solution ...
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bioRxiv - Cell Biology 2022Quote: ... the mixes were boiled at 97°C for 5 min and loaded in a Mini-PROTEAN® TGX™ Precast Gel (Biorad, Hércules, EE.UU) at 12% acrylamide/bis-acrylamide ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of each cell protein lysate sample was separated using a hand-cast 5-15% gradient Tris glycine gel (Bio-Rad, Protocol Bulletin 6201) using a custom made 25-well comb.
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted at a concentration of 5 ng/µl and 2 µl were loaded in duplicate in a 96-well qPCR plate with the reaction mix comprising 5 µl of Sybr Green master mix (Bio-Rad, Hercules, CA, USA), 2 µl of water and 1 µl of primer mix containing forward and reverse primers for STAT3 and P53 ...
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bioRxiv - Molecular Biology 2024Quote: ... The same AAV2 ITR primers were used for ddPCR along with a FAM labeled probe 5 ’-CACTCCCTCTCTGCGCGCTCG-3 with the QX200 Bio-rad Laboratories’ droplet digital system (Bio-rad Laboratories, California, USA) and ddPCR supermix for probe (Bio-rad Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... 250 μL of the mixed cell suspension was chilled on ice for 5-10 minutes in a 4-mm wide cuvette compatible with the Gene Pulser®/MicroPulser™ (BioRad, Hercules, CA). The cell mixture was electroporated using the GenePulser XCell™ device (BioRad ...
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bioRxiv - Immunology 2024Quote: ... The 1:10 diluted plasmid was combined with C41 cells (∼3 x 109 competent cells) and incubated on ice for 5 min followed by electroporation using a Bio-Rad Electroporator (Bio-Rad, Hercules, CA, USA). The transformation was incubated at 37°C in LB media for 1 hr prior to spreading onto LB agar plates containing kanamycin ...
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bioRxiv - Immunology 2024Quote: ... Proteins were denatured at 94°C for 5 min before separation on SDS-PAGE (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels were used (Bio-Rad Laboratories)) ...
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bioRxiv - Plant Biology 2024Quote: ... Samples equivalent to 1 µg chlorophyll were solubilized with 2X sample buffer (Laemmli buffer + 6M urea + β-mercaptoethanol + Bromophenol blue) for 5 min at 75°C and loaded onto 12% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad). After protein separation ...
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bioRxiv - Molecular Biology 2024Quote: ... a mixture of 250 μL of this cell suspension and 1000 ng of a vector plasmid digested with XbaI and KpnI enzymes was chilled on ice for 5–10 minutes within a 4-mm width cuvette compatible with the Gene Pulser®/MicroPulser™ system (BioRad, Hercules, CA). The cell-DNA mixture was then electroporated using the GenePulser XCell™ device (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... and boiled for 5 min before loading into a 12% or 4-20% acrylamide pre-cast SDS- PAGE gels (Bio-Rad, 4561045 or 4568094) for electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... A 5-μL aliquot of the pre-amplified reaction was analyzed by qPCR using SsoFast EvaGreen Supermix (Bio-Rad Life Science, Inc., Hercules, CA, USA) and an Applied Biosystems 7900HT qPCR instrument (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... and were heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (Bio-Rad, 4–15% Tris/Glycine) and then transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... exposed to enhanced chemiluminescence plus west pico for 5 min and autoradiographed in a BioRad Gel-Doc System (Bio-Rad Laboratories Inc., Hercules, CA, USA). The loading consistency was verified utilizing the house-keeping proteins (Rab11 and β-Actin ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were dissolved in 25 μL of double distilled water (boiled at 95 °C for 5 minutes) containing loading buffer (4X; Bio-Rad Laboratories, Mississauga, ON, Canada) and reducing agent (20X ...
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bioRxiv - Microbiology 2024Quote: ... Electroporation was carried out by mixing 45 µl of competent cells with 100-200 ng of plasmid and subjecting the cells to a short 5 ms electric pulse under a 12500 V/cm electric field in a Gene Pulser II® (Bio-Rad, Hercules, CA, USA) (2500 v ...
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bioRxiv - Plant Biology 2022Quote: ... Amplification of target amplicons with the addition of 5’ XbaI and 3’ BamHI restriction enzyme digestion sites was carried out on a Bio-Rad T100 thermocycler (Bio-Rad Laboratories, Hercules CA, USA) via Bio-Rad iProof High Fidelity DNA Polymerase using manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Tungsten beads were coated with 5 µg of plasmid shot on the plates using the PDS-100/He system (Bio-Rad,Hercules, CA, USA; 1672257) with 1550 psi rupture disks ...
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bioRxiv - Neuroscience 2024Quote: ... First strand cDNA was diluted 1:1000 and 5 μL was used as template in droplet digital PCR (ddPCR) using ddPCR Supermix for Probes (no dUTP; Bio-Rad, Des Plaines, IL, USA) and TaqMan Gene Expression Assay probes (Applied Biosystems ...
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bioRxiv - Genetics 2024Quote: ... 30μg total protein from tissue samples with 4X Laemmli buffer was heat denatured at 99°C for 5 minutes and ran on resolving SDS PAGE gel (Biorad 4-20% precast gel #4561096). Sperm protein samples were prepared using a low volume of lysis buffer (∼100) ...
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bioRxiv - Plant Biology 2021Quote: GV3101 electro-competent Agrobacterium tumefaciens strain was incubated on ice with ~100 ng plasmid for 5 min and electroporated using a MicroPulser (Bio-Rad Laboratories; 2.2 kV, 5.9 ms). Immediately after electroporation ...
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bioRxiv - Biochemistry 2021Quote: ... Beads were incubated 5 min at 95 °C in this solution and 20 µL of supernatant was analyzed on gradient gels (BioRad 4-12% TGX pre-cast gels). The modified proteins were detected with an infrared imager (Odyssey ...
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bioRxiv - Neuroscience 2021Quote: ... qRT-PCR was performed using iQ™ SYBR®Green Supermix on an iQ™5 multicolor real-time PCR detection system (Bio-Rad, CA, USA). All primers were obtained from Sigma-Aldrich and described in Suppl ...
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bioRxiv - Plant Biology 2024Quote: ... Six μL cDNA (diluted 1:5 with distilled water) was used per qRT-PCR reaction in a 15-μL reaction volume using SYBR Green (BIO-RAD, iTaq Universal SYBR Green Supermix) and a BIO-RAD CFX96 real time PCR instrument ...
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bioRxiv - Biochemistry 2021Quote: ... All final samples were boiled in 30 µL NuPAGE LDS sample buffer and sample reducing agent for 5 min and loaded on Mini-PROTEAN® TGX Stain-Free™ Gels (Bio-Rad laboratories, Hercules, CA, USA). Electrophoresis was performed at 300 V for 3 min using the BIO-RAD Mini-PROTEAN® Tetra System ...