Labshake search
Citations for New England Biolabs :
4801 - 4850 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... 53.5 μl of cut 3C library was mixed with 6.5 μl NEBNext FFPE Repair Buffer and 2 μl NEBNext FFPE Repair Mix (New England Biolabs), followed by incubation at 20°C for 15 minutes and addition of 3 volumes of AMPure XP beads for purification.
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bioRxiv - Genomics 2020Quote: ... Primer mixes were diluted 1:25 and 1μl of each mix was used for overlap-extension PCR using Phusion (NEB). Four 50μl reactions for each mix were performed using cycling conditions 98°C 1min ...
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bioRxiv - Cell Biology 2021Quote: Whole cell lysates or eluted proteins after affinity purification were treated with Protein Deglycosylation Mix II (NEB, Cat# P6044), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... PCR fragments were inserted into linearized NaV channel expression plasmids by Gibson Assembly using NEBuilder HiFi Assembly Mix according to the manufacturer’s protocol (New England BioLabs). Introns were inserted after c.2946 (exon 15/16 junction ...
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bioRxiv - Plant Biology 2020Quote: ... 100ng of each entry and destination vector are assembled in one reaction mix containing 10U BsaI-HF-v2 (NEB), 200U T4 Ligase (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... DGRC) downstream of and in-frame with the 3X FLAG tag using Gibson assembly (HiFi DNA assembly mix, NEB). Expression of FLAG-tagged DNMT2 in fly cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... benthamiana using the method described in the immunoblotting section were treated with Protein Deglycosylation Mix II (New England BioLabs) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... before incubation during 6 min in 50μl of PNK reaction mix (1x PNKT, 1 mM ATP and 0.05 U/ml T4 PNK 3′phosphatase minus (NEB) in a thermomixer at 37°C and 1400rpm ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized from 0.5-1 µg of RNA per sample using Protoscript II RT and Random Primer Mix (New England Biolabs). qPCR reactions were performed on cDNA using iQ SYBR Green supermix (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified small RNA samples were then digested using Nucleoside Digestion Mix (catalog no. M0649S, New England BioLabs, Beverly, MA). LC-MS/MS measurement of various post-transcriptional modifications were performed on a Waters XEVO TQ-STM (Waters ...
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bioRxiv - Genomics 2020Quote: ... tipulae DNA and control DNA (lambda 3,2 kb fragment) were subjected to DNA damage repair (NEBNext FFPE DNA Repair Mix; New England Biolabs) followed by DNA End Repair (NEBNext Ultra II End Repair/ dA-tailing Module ...
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bioRxiv - Genomics 2022Quote: ... Next, 500 nL of MspJI digestion mix (1x NEBuffer 4, 8x enzyme activator solution, 0.1 U MspJI (NEB, R0661L)) was added to each well and the plates were incubated at 37°C for 4.5 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions contained approximately 5 μL of isothermal assembly mix (prepared similar to as previously described12) or NEBuilder HiFi (NEB), 0.01 pmol of plasmid linearized via SpRYgest ...
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bioRxiv - Microbiology 2021Quote: ... First strand cDNA synthesis was done by incubating 1 μg total RNA with 2.5 mM Oligo dT(18) and 0.5 mM dNTP mix (NEB, N0447L) for 5 min at 70°C before placing on ice for 2 min ...
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bioRxiv - Microbiology 2021Quote: ... DGRC) downstream of and in-frame with the 3X FLAG tag using Gibson assembly (HiFi DNA assembly mix, NEB). Expression of FLAG-tagged DNMT2 in fly cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... 100ng of each entry and destination vector are assembled in one reaction mix containing 10U BsaI-HF-v2 (NEB), 200U T4 Ligase (NEB) ...
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bioRxiv - Plant Biology 2022Quote: ... each well containing 0.8 µL Primer Master Mix (0.225% Triton X-100, 1.6 mM dNTP mix, 1.875 uM barcoded oligo[dT] CEL-seq2 primers; Sigma-Aldrich, New England Biolabs) using a BioSorter (Union BioMetrica ...
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bioRxiv - Systems Biology 2019Quote: ... the samples were added to 2 μL of second-strand synthesis mix (2.5× NEB buffer 2 [New England Biolabs] ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10x diluted T7 RNA Polymerase mix (HiScribe™ T7 High Yield RNA Synthesis Kit, New England Biolabs, USA). After linear amplification ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10x diluted T7 RNA Polymerase mix (HiScribe™ T7 High Yield RNA Synthesis Kit, New England Biolabs, USA). After linear amplification ...
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bioRxiv - Molecular Biology 2021Quote: ... washed with 100 μl of 1x MNase Buffer and incubated in 100μl of MNase reaction mix (1x MNase buffer and 30 gel unit/μl MNase (New England Biolabs)) during 3 min at 37°C with mix at 1400rpm ...
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bioRxiv - Genomics 2021Quote: ... The following day samples were digested with 20 μl of a NIaIII and PstI enzyme mix (New England Biolabs) in NEB Cutsmart Buffer at 37 °C for 2 h ...
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bioRxiv - Genetics 2020Quote: ... The annealed sgRNA oligos were ligated with BbsI-linearized Cas9 construct using Blunt/TA ligation mix (New England Biolabs). For the sgRNA targeting the rs2435357 locus ...
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bioRxiv - Microbiology 2020Quote: ... 2μL of the resulting mix was transformed into chemically competent Escherichia coli (High efficiency DH5-alpha, New England Biolabs) according to manufacturer’s instructions and cultured for 16 hours on Luria broth (LB ...
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bioRxiv - Genomics 2021Quote: ... The sample was then allowed to cool to room temperature and incorporated in the Bst 2.0 enzyme mix (1X Isothermal amplification buffer, 1mM dNTP, 1U/μL of Bst 2.0 DNA polymerase; NEB M0537L) was added to the denatured library and the mixture was incubated at 55 °C for 10 mins and 60 °C for 45 minutes ...
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bioRxiv - Genomics 2021Quote: ... The DNA samples were DNA repaired and end-prepped using the NEBNext FFPE DNA Repair Mix (New England Biolabs) and NEBNext End repair/dA-tailing Module (New England Biolabs) ...
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bioRxiv - Genomics 2022Quote: ... MA) and then subjected to DNA repair by NEBNext FFPE DNA Repair Mix (Catalog No. M6630L, NEB, Ipswich, MA) and dA tailing by NEBNext Ultra II End-Repair/dA-tailing Module (Catalog No ...
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bioRxiv - Evolutionary Biology 2022Quote: 75ng of genomic DNA were used for PCR amplification in two 50µl reactions with 25µl 2xQ5 mix (NEB M0492), 2.5µM of each forward (FOS ...
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bioRxiv - Synthetic Biology 2022Quote: ... An Esp3I restriction site was introduced immediately upstream of the CHIPΔTPR CDS and GSGSG linker via the KLD Enzyme Mix (NEB) following PCR amplification with mutagenic primers (Genewiz) ...
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bioRxiv - Microbiology 2022Quote: ... Deglycosylation of purified protein was performed in non-denaturing conditions according to manufacturer’s protocol (Protein Deglycosylation Mix II, NEB).
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bioRxiv - Microbiology 2022Quote: ... Point mutations or small deletions in pGM34 and pGM71 (Table 1) were performed using the KLD enzyme mix (NEB)
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bioRxiv - Plant Biology 2022Quote: ... and the BioNotI-P5-PET oligo and captured by Dynabeads for end repairing with End Repair Enzyme Mix (NEB) and ligation with barcoded Illumina compatible adapter using T4 DNA Ligase (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg non-methylated NEAT1_1 IVT product was radio-labelled with radioactive labelling mix (1 µL 10x PNK buffer, NEB, 1 µL NEAT1_1 IVT product ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg non-methylated NEAT1_1 IVT product was radio-labelled with radioactive labelling mix (1 µL 10x PNK buffer, NEB, 1 µL NEAT1_1 IVT product, 1 µL T4 PNK, NEB, 1 µL γ-32P-ATP ...
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bioRxiv - Molecular Biology 2024Quote: ... Cellular and EV RNAs were enzymatically digested to single nucleosides under neutral conditions using the Nucleoside Digestion Mix (BioLabs). RNA nucleosides were combined with 1 nM of [15N]-dA internal standard immediately prior to analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... A-tailing was performed by addition of 5 µL of the A-tailing mix (0.5 µL of Cutsmart buffer 10X, 0.25 µL Klenow 50 U/µL (NEB, M0212M), 0.05 µL dATP 100 mM ...
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bioRxiv - Microbiology 2024Quote: ... Fragmented DNA samples were prepared for Illumina sequencing using the NEBNext Ultra I DNA Library Prep Kit for Illumina according to manufacturer’s instructions with the following modifications: ends of fragmented DNA were repaired using the End Prep Enzyme Mix (NEB) and an NEBNext adaptor for Illumina sequencing was ligated to the newly repaired ends ...
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bioRxiv - Developmental Biology 2024Quote: ... a mix containing 18µM sgRNA and 12µM Cas9 protein (EnGen™ Spy Cas9-NLS S. pyogenes, NEB Cat# M0646T) was incubated for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... Total cDNA for RT-qPCR was generated from 1.5 µg total RNA using a random primer mix and M-MuLV reverse transcriptase (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... 30 µL of the eluted DNA samples were mixed with 7 µL of TSO digestion mix (3.5 µL UDG-DNA glycosylase and 3.5 µL UDG Buffer, NEB M0280S) and incubated at 37 ºC for 30 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... AMX sequencing adaptors were ligated by mixing 2.5 ul of the assembly mix with 5 ul AMX and 5 ul Blunt/TA mastermix from NEB and incubated for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were re-suspended in 100µl PNK-mix (70mM Tris-HCl pH6.5, 10mM MgCl2, 1mM DTT, 100U RNasin, 1U TurboDNase, 25U Polynucleotide-Kinase (NEB)) and incubated for 20min at 37°C in a thermomixer with shaking (1200 x rpm) ...
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bioRxiv - Genetics 2023Quote: ... were ligated overnight at 20°C with a mix of six 5’ phosphorylated telorette oligonucleotides (0.1 µM each) and 2,000 units of T4 ligase (NEB Inc.) in a 50 µl reaction ...
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bioRxiv - Developmental Biology 2023Quote: ... 3 μL of Round1 barcode mix (1x T4 DNA ligase buffer, 16 M/μL T4 DNA ligase (M0202L, NEB), 0.25 M/μL RNase Inhibitor ...
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bioRxiv - Molecular Biology 2022Quote: ... A-tailed fragments were ligated to 50 nL of 5 mM T7 promoter containing adapters(21) with cell barcodes and UMI with 150 nL ligation mix (25 nL T4 DNA ligase (400.000U, NEB), 3.5 nL MgCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 8 μl of a mix preheated to 37°C and containing 2.5 U of RNase H (New England Biolabs), 2.5× RNase H reaction buffer (1× buffer ...
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bioRxiv - Biochemistry 2023Quote: ... and then digested from RNA to nucleosides using a Nucleoside Digestion Mix kit (New England Biolabs Inc., Massachusetts, USA) at 37 °C for 1 h ...
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bioRxiv - Bioengineering 2023Quote: ... An Esp3I restriction site was introduced immediately upstream of the CHIPΔTPR CDS and flexible GSGSG linker via the KLD Enzyme Mix (NEB) following PCR amplification with mutagenic primers (Genewiz) ...
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bioRxiv - Molecular Biology 2023Quote: ... 7.5 µL DNA was incubated (14 h, 37°C) with IVT mix (0.5 µL Ribolock inhibitor, Invitrogen, 2 µL T7 polymerase buffer, NEB, 8 µL rNTP mix ...
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bioRxiv - Molecular Biology 2023Quote: ... 37°C with rotation) in 100 µL MNase reaction mix (87 µL ddH2O, 10 µL 10x MNase buffer, NEB, 1 µL 100x BSA ...