Labshake search
Citations for New England Biolabs :
4751 - 4800 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Beads were reinjected at 75 μl/h and co-flowed with 150 μl/h RT mix (1.65x Maxima H-RT Buffer, 0.033 U/μl USER II (NEB), 15 U/μl Maxima H-RT (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... To start the first round of ligation 40 μl of ligation mix (9.54 U/μl T4 ligase (NEB) in 1x T4 ligase buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Paired barcode and splints were phosphorylated in a PCR plate by combining 20 μl of oligonucleotides with 40 μl phosphorylation mix (1X T4 ligation buffer, 0.2 mg/ml BSA, 0.167 U/μl T4 PNK (NEB)) and incubated for 30 min at 37°C and heat inactivated for 20 min at 65°C ...
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bioRxiv - Microbiology 2019Quote: ... PCR products of uvrC or anmK were then mixed with the 3.0- and 5.5-kB fragments of pME6010 and assembled using the NEBuilder HiFi mix (NEB). Plasmids were transformed into E ...
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bioRxiv - Genomics 2021Quote: dATP was added to the 3’ ends by adding 9μL of A-tailing mix (5μL NEB buffer 2.1, 1μL of 1mM dATP, 5U Klenow exo (NEB M0212S)) to the 41μL of DNA sample from the previous step ...
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bioRxiv - Genomics 2021Quote: ... dATP was added to the 3’ ends by adding 9μL of A-tailing mix (5μL NEB buffer 2.1, 5μL of 1mM dATP, 3U Klenow exo (NEB M0212S)) to the 41μL of beads with bound DNA from the previous step ...
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bioRxiv - Genomics 2021Quote: The beads were magnetically separated and 4 µl of hot PNK mix (0.2 µl PNK [New England Biolabs] ...
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bioRxiv - Genomics 2021Quote: ... 50 µl of ULR-Phusion mix (1.2 µl ULR Ladder, 28.8 µl H2O, 30 µl Phusion PCR mastermix [New England Biolabs] and the samples were mixed with 147.5 µl ProNex chemistry ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1× NEB 1 buffer ...
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bioRxiv - Cell Biology 2020Quote: 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1x NEB 1 buffer ...
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bioRxiv - Genomics 2019Quote: ... After 2 rounds of SPRI cleanup the libraries were eluted in EB buffer and USER enzyme mix (NEB) was used to digest the second strand cDNA ...
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bioRxiv - Microbiology 2021Quote: Expression plasmids (Supplementary Table T 2) were cloned by isothermal assembly using NEBuilder HiFi DNA Assembly Mix (NEB). Plasmids were transformed into E ...
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bioRxiv - Cell Biology 2021Quote: ... The volume of the reaction mix was completed to 30 μl with the MetalloPhosphatase buffer (New England Biolabs) containing 1 mM of MnCl2 prior to a 30 min incubation at 30°C ...
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bioRxiv - Microbiology 2020Quote: ... Fragments were end-repaired using the NEBNext® FFPE DNA Repair Mix (New England Biolabs, Ipswich, MA, USA), with subsequent fragment purification using AMPure XP beads (Beckman Coulter Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... 500 ng of total RNA was mixed with 2 µL of random primer mix (New England Biolabs, UK) in RNase free PCR strips (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: The PCR reactions were prepared as follows: Mix 1 μl of Phusion DNA Polymerase (New England Biolabs, M0530), 10 μl of 5x Phusion HF Buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... CaMKII substrates were added to the 5’ end of cpGFP by site-directed mutagenesis (KDL Enzyme Mix, NEB). deadCaMKART6A was generated by site-directed mutagenesis of pcDNA3.1:CMV-CaMKAR ...
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bioRxiv - Developmental Biology 2024Quote: ... gel purified and transcribed with DIG RNA labeling mix with either T7 RNA polymerase (NEB, M0251S; antisense probe), or SP6 RNA polymerase (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... <1 ng of DNA of interest was pipetted to a mix of 1x rCutsmart buffer (New England Biolabs), 100 μM of primer ...
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bioRxiv - Genomics 2023Quote: ... Gaps in the transposed DNA were filled by 15μL gap-fill mix (7 μL Q5 reaction buffer (NEB), 7 μL Q5 high GC enhancer (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified and filtrated RNA was then digested to single nucleoside by Nucleoside Digestion Mix (M0649S, NEB, Ipswich, MA). LC-MS/MS analysis was performed by injecting digested stable isotope labeled RNAs on an Agilent 1290 Infinity II UHPLC equipped with a G7117A diode array detector and a 6495C triple quadrupole mass detector operating in the positive electrospray ionization mode (+ESI) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... paraffin-embedded (FFPE) DNA repair step was first performed using NEBNext FFPE DNA Repair Mix (New England BioLabs). A 10-fold dilution of adaptors was used ...
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bioRxiv - Genomics 2023Quote: Cells were resuspended in 400 μl of restriction enzyme (RE) digestion mix (1x T4 ligase buffer (NEB, B0202S), 500U MseI (NEB ...
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bioRxiv - Genomics 2023Quote: ... Second strand synthesis was performed after thawing by the addition of 5 µL of second strand synthesis mix (3 µL of elution buffer [Qiagen], 1.33 µL mRNA second strand synthesis buffer and 0.66 µL of second strand synthesis enzyme mix [NEB]) and incubated at 16°C for 3 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was carried out with 1.5 mM (high concentration) or 0.1 mM (limiting concentration) dNTP mix and 2.5 U AMV-RT (NEB, M0277S) and 1 hour incubation at 42°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... 0.5 μL BsaI-HF® v2 NEBridge® Golden Gate Assembly mix (E1601, NEB, 5 μL final volume), and 0.5 μL T4 DNA ligase buffer was aliquoted across a PCR plate ...
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bioRxiv - Biochemistry 2023Quote: ... and then deglycosylated and dephosphorylated overnight at 37 °C with the protein deglycosylation mix II (New England Biolabs) and Quick CIP (New England Biolabs) ...
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bioRxiv - Genetics 2023Quote: ... (1.33x Gibson-Assembly Master-Mix [for 25 reactions]: 100 μL 5x Isothermal Reaction-Mix, 215.32 μL H2O, 0.2 μL T5 Exonuclease [10 U/μL NEB #M0363] ...
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bioRxiv - Plant Biology 2023Quote: ... Adapter Mix II (ONT, Oxford, UK), ONT Ligation Buffer (ONT, Oxford, UK) and Quick T4 DNA Ligase (NEB) were added and incubated for 10 minutes at room temperature and purified with 0.4X AMPure XP beads (Beckman Coulter ...
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bioRxiv - Plant Biology 2023Quote: ... The genomic DNA was barcoded, adding Native Barcoding Expansion (ONT, Oxford, UK) and Blunt/TA ligase mix (NEB) was added for 10 minutes at room temperature and purified with 1X AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Genomics 2023Quote: ... Transposed DNA was gap-filled by addition of 4μL gap-fill mix (3.2 μL Q5 reaction buffer (NEB), 0.07 μL 1M MgCl2 ...
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bioRxiv - Genomics 2023Quote: ... The end-tagged DNA was oxidized in 15 μL BGT reaction mix (0.3 μL Uridine Diphosphate Glucose (NEB), 1.5 μL NEBuffer 4 (NEB) ...
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bioRxiv - Genomics 2023Quote: ... Gaps in the transposed DNA were filled by 4μL gap-filling mix (3.2 μL Q5 reaction buffer (NEB), 0.07 μL 1M MgCl2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... was then done with the homologies to construct the initial green fluorescent protein (GFP) plasmid and then transformed into competent E.coli (NEB#C2984H) and subsequently sequenced (Plasmidsaurus ...
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bioRxiv - Genomics 2023Quote: ... the SNAP/Halo-positive clone cultured on coverslips (Matsunami Glass) were treated with HaloTag Oregon Green and SNAP-Cell TMR-Star (New England Biolabs) according to manufacturers’ protocols and fixed with 4% PFA in PBS at room temperature for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... by replacing the green fluorescent protein (GFP) with a mCherry reporter using the NEBuilding HiFi DNA Assembly Cloning Kit (New England Biolabs) (Ran et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... The pRL814 (containing green fluorescent protein (GFP) under control of PT7A1-O34) backbone was digested with NdeI and HindIII-HF (NEB). The digested backbone and amplified genes were ligated using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... Gene-specific LA-qPCR analyses for measuring DNA damage were performed using Long Amp Taq DNA polymerase (New England Biolabs, Cat no MO323S). Since amplification of a small region would be independent of DNA damage ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription and quantitative PCR were performed in one step using the Luna Universal One-Step RT-qPCR Kit (New England BioLabs, Ipswich, MA, USA). The primers used are listed in supplemental table (Table ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 viral RNA detection and quantification was performed using the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs, Whitby, ON, Canada) on the Rotor-gene Q platform (Qiagen) ...
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bioRxiv - Genetics 2021Quote: ... 1μl XhoI digestion mix consisting of 5U XhoI restriction enzyme and 1 x NEB buffer 2.1 (New England Biolabs), and 10 ng genomic DNA for iciHHV-6B samples or 200 ng DNA for non-iciHHV-6B samples ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 ng total RNA in a volume of 9 μl was ligated to 1 μl custom adapter mix (18S:28S ratio 1:2) using 1.5 μl T4 DNA ligase (New England Biolabs) in NEB next Quick ligation buffer (3 μl ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA-bound beads were incubated for 30 min at 37°C in 100 μl A-tailing mix (1x NEB buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... and directly used for in vitro transcription of single-guide RNAs (sgRNAs) with a T7 Polymerase mix (M0255A NEB). All sgRNA reactions were treated with RNAse free-DNAse ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA-bound beads were incubated for 30 min at 37°C in 100 μl A-tailing mix (1x NEB buffer, 0.5 μM dATP, and 0.25 U/ μl Klenow, exo-(NEB, M0212)) ...
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bioRxiv - Genomics 2020Quote: ... were combined with RT primer mix [1 μl 250 ng/μl randomhexRT primer and 0.5 μl 10 mM dNTPs (NEB)] ...
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bioRxiv - Genomics 2019Quote: ... 3.5μL of Ultra II End-prep reaction buffer and 3μL of Ultra II End-prep enzyme mix (all from New England Biolabs); the mixture was incubated at 20°C for 15 minutes and 65°C for 15 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... The DAC mutants were further purified and cloned to the digested p38d vector using NEB builder Gibson mix (NEB). The V5 mutant was ordered as a synthetic gene ...
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bioRxiv - Genomics 2019Quote: ... and ligated to 1.5 µL of the 25 µM annealed adapter mix (NEBNext Ultra Ligation Module, New England Biolabs). Products larger than 400 bp were purified by gel excision (using NuSieve ...
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bioRxiv - Microbiology 2019Quote: ... of desired templates were in vitro-transcribed overnight at 37°C with 2.5 mM Ribonucleotide Solution Mix (New England Biolabs) and when needed ...