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Citations for New England Biolabs :
401 - 450 of 642 citations for Rubella Spike Glycoprotein E1 strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... pH 7.6 and incubated overnight with 2 µl PNGase F (New England BioLabs) and 0.5 µl sialidase A (Prozyme) ...
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bioRxiv - Molecular Biology 2020Quote: ... egg coats were treated with PNGase F (New England BioLabs, Ipswich, MA, USA) prior to electrophoresis following the manufacturer’s protocol.
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bioRxiv - Genetics 2022Quote: ... samples were treated with PNGase F (P70404L, New England Biolabs, Ipswich, MA, USA) or Endo H (P0702 ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of the lysates were incubated with 2500 units of PNGase F (NEB) at 37°C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: HeLa cell lysates were incubated with PNGase F (New England Biolabs, NEB-P0704S) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: HeLa cell lysates were incubated with PNGase F (New England Biolabs, NEB-P0704S) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... N-glycans were enzymatically removed from the tryptic peptides with PNGase F (NEB) overnight at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... were added with 1.5 µL of 10× PNGase F buffer (New England Biolabs) in the sample by adjusting with Milli-Q H2O to total 15 µL ...
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bioRxiv - Bioengineering 2024Quote: ... were each treated with a mixture of PNGase F (NEB, Boston, MA, USA) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... The Flag-tagged spike gene was ligated to the linearized vector using the T4 DNA Ligase enzyme from NEB. The resulting plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... and T478K of the Delta variant was spliced into the spike expression vector by HiFi DNA assembler cloning (NEB). The plasmid was prepared and verified as described above.
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bioRxiv - Microbiology 2021Quote: ... without codon optimization and was inserted into pcDNA 3.1 to g et pcDNA 3.1-SARS-CoV-2-Spike using NEBuilder® HiFi DNA Assembly Master Mix (NEB) a ccording to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The C-terminal Flag tag was introduced into pcDNA3.1-Spike-WT thanks to NEBuilder® HiFi DNA Assembly (NEB). The Flag tag was added during amplification of Spike-WT from pcDNA3.1-Spike-WT with the primers ...
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bioRxiv - Synthetic Biology 2019Quote: The bacterial strains used in this study includes Escherichia coli DH5α (New England Biolabs), Escherichia coli Nissle 1917 (isolated from probiotic Mutaflor capsule) ...
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bioRxiv - Biochemistry 2021Quote: ... Erythromycin-sensitive strains were tested with colony PCR (OneTaq DNA polymerase, New England Biolabs) using the clpP2_KO_A and clpP2_KO_D primer pair (Table 3 ...
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bioRxiv - Biochemistry 2021Quote: ... Erythromycin-sensitive strains were tested with colony PCR (OneTaq DNA polymerase, New England Biolabs) using the clpP_tag fwd and rev primer pair (Table 2 ...
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bioRxiv - Microbiology 2021Quote: ... coli JP313 ΔtolC strains using a Q5 high-fidelity DNA PCR (New England Biolabs). The primers used for these reactions were “EM029-TMKF1” (5’-ATGGCAAACTTTCTCGTG-3’ ...
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bioRxiv - Microbiology 2022Quote: ... The Escherichia coli strain used for routine cloning was NEB 5α (New England Biolabs). The plasmids used in this article are listed below (Table S2) ...
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bioRxiv - Biophysics 2019Quote: ... This vector was transformed into BL21 (DE3) strain (New England BioLabs France, Evry, France). The transformed cells were inoculated into 1 liter of LB medium containing 50μg/ml kanamycin sulfate ...
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bioRxiv - Microbiology 2022Quote: ... coli host strain using the Monarch® Plasmid Miniprep Kit (NEB, Ipswich, MA, USA). The extracted plasmid was linearized with restriction enzyme BamHI (Promega ...
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bioRxiv - Microbiology 2023Quote: ... was amplified from Ap strain HZ gDNA using Q5 DNA polymerase (New England Biolabs). Cloning into pET-28a and a modified pET41 was performed using restriction free PCR-based methods26 ...
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bioRxiv - Biochemistry 2024Quote: Plasmids required for generating mutant strains were prepared using HiFi DNA assembly (NEB, USA) and then transformed into S ...
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bioRxiv - Molecular Biology 2021Quote: ... cell lysates were incubated in 40 μg/ml PNGase F (New England Biolabs, MA) for 2 hr at 37 °C to cleave all N-glycans before immunoblotting 62 ...
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bioRxiv - Cell Biology 2020Quote: ... and digested with EndoH or PNGase F per the manufacturer specifications (New England BioLabs)
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bioRxiv - Cancer Biology 2019Quote: ... reduced and alkylated and proteins were digested with first PNGase F (New England BioLabs), endoproteinase Lys-C (Promega ...
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bioRxiv - Microbiology 2021Quote: ... the samples were incubated at 37°C overnight with PNGase F (New England BioLabs) to release the glycans ...
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bioRxiv - Immunology 2022Quote: ... 125mM DTT) or further subjected to de-glycosylation with PNGase-F (NEB, Ipswich, MA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Deglycosylation of the DENV was performed using PNGase F (New England Biolabs, Ipswich, MA) and Endo H (New England Biolabs ...
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bioRxiv - Neuroscience 2019Quote: ... sEV samples and total homogenates (TH) were digested with PNGase F (New England Biolabs) according to the manufactureŕs protocol.
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were additionally treated with 16 units of PNGase F (New England Biolabs) per μL of lysate complemented with 1% NP-40 ...
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bioRxiv - Immunology 2022Quote: ... 10 μg Rtx and Hct were digested by 500 unit PNGase F (NEB, USA) at 37 °C for 24 h under native conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... this was followed by incubation with 1 µl PNGase F (NEB, Ipswich, MA, USA), 2 µl 10% NP-40 (NEB ...
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bioRxiv - Immunology 2021Quote: The DNA sequences of B.1.351 and B.1.617 SARS-CoV-2 spikes for the mRNA transcription and pseudovirus assay were synthesized as gBlocks (IDT) and cloned by Gibson Assembly (NEB) into pcDNA3.1 plasmids ...
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bioRxiv - Microbiology 2021Quote: ... The amino acid deletions in the full-length SARS-CoV-2 Spike expressor were generated using the Q5 site-directed mutagenesis kit (NEB). The presence of the desired mutations was determined by automated DNA sequencing ...
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bioRxiv - Microbiology 2022Quote: ... Mutations were introduced into the phCMV-Spike plasmids using the mutagenic primers and the Q5 site directed mutagenesis kit (NEB). In addition ...
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bioRxiv - Molecular Biology 2020Quote: The bacterial source strains used for this work were Escherichia coli NEB5α (New England BioLabs) for plasmid constructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli strain DH5α was used for plasmid construction while SHuffle T7 Express (New England Biolabs) 20 was used for cyclonal expression and library selections ...
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bioRxiv - Microbiology 2020Quote: The bacterial source strains used in this work were Escherichia coli NEB5α(New England Biolabs) for plasmid constructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... then transformed into the E.coli NEB Stable strain from New England Biolabs (NEB, MA, USA) for plasmid isolation ...
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bioRxiv - Cell Biology 2020Quote: ... These strains were transformed by electroporation [20] with the EcoNI-linearized (R0521S; New England Biolabs) pRK2 plasmid ...
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bioRxiv - Microbiology 2021Quote: ... The protein expression level was evaluated in four T7 expression strains: T7 Express (C2566, NEB), T7 Express with LysY and lacIq (C3013 ...
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bioRxiv - Microbiology 2022Quote: ... coli strain used in this study for cloning purposes was NEB-5α (New England Biolabs) (Table 1) ...
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bioRxiv - Plant Biology 2023Quote: ... and affinity purified from Escherichia coli strain BL21 (DE3) using Amylose Resin (New England Biolabs) as previously described 5.
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bioRxiv - Molecular Biology 2023Quote: ... The assembled plasmid was introduced into Escherichia coli strain NEB 5-alpha (New England Biolabs) by heat shocking ...
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bioRxiv - Bioengineering 2022Quote: ... coli NEB 10-β and BL21 (DE3) strain (both from New England BioLabs, Ipswich, USA) with pRI952 plasmid used for all fermentations ...
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bioRxiv - Microbiology 2020Quote: ... stained with a mAb cocktail composed of SARS-CoV-2 spike (Creative-Bios; 2BCE5) and SARS-CoV-2 nucleoprotein (Creative-Biolabs; NP1C7C7) followed by anti-Mouse IgG-HRP (Abcam ab6823 ...
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bioRxiv - Immunology 2022Quote: ... The spike plasmids were linearized by restriction enzymes and transcribed to mRNA by in vitro T7 RNA polymerase (NEB, Cat # E2060S) as previously described7,8.
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bioRxiv - Biochemistry 2020Quote: N-linked glycans were released from gp140 in-gel using PNGase F (New England Biolabs). The released glycans were subsequently fluorescently labelled with procainamide using 110mg/ml procainamide and 60mg/ml sodium cyanoborohydride ...
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bioRxiv - Biochemistry 2019Quote: ... pH 8.0 and deglycosylated with 500 U of PNGase F (New England Biolabs, Ipswich, MA) and 0.1 U Sialidase A (Prozyme ...
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bioRxiv - Cell Biology 2021Quote: The presence of N-linked oligosaccharides was examined using PNGase F (New England Biolabs (NEB), Ipswich ...