Labshake search
Citations for New England Biolabs :
301 - 350 of 642 citations for Rubella Spike Glycoprotein E1 strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... both proteins were deglycosylated by PNGase F (NEB, 1:50) overnight at 37°C in PBS ...
-
bioRxiv - Cell Biology 2019Quote: ... 1% NP40 and PNGase-F (50 U/μl: NEB#P0704S).
-
bioRxiv - Bioengineering 2020Quote: Antibodies were first digested with PNGase F (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The protein was digested with PNGase F (New England BioLabs) under denaturating conditions ...
-
bioRxiv - Microbiology 2021Quote: F(ab’)2 fragments were generated using IdeZ protease (NEB), purified using CaptureSelect LC-lambda affinity matrix (human ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein/lysate sample de-N-glycosylation using PNGase F (NEB), Endo S (NEB) ...
-
bioRxiv - Biophysics 2022Quote: ... 2 μL (1000 U) PNGase F (New England Biolabs Inc) were added and the reaction was incubated for 6 h at 37 °C ...
-
bioRxiv - Systems Biology 2019Quote: ... immunoprecipitated receptors were deglycosylated with PNGase F (New England Biolabs), before resuspending in LDS sample buffer and running on NuPAGE 4-12% Bis Tris precast gels ...
-
bioRxiv - Cell Biology 2019Quote: ... Deglycosylation assay using PNGase F (P0704S, New England BioLabs Inc.) was performed according to manufacturer’s protocol with the exception of incubating the reaction for 3 hours at 37°C followed by overnight at RT ...
-
bioRxiv - Biochemistry 2020Quote: ... Samples treated with PNGase F (New England Biolabs, Ipswich, MA) were treated according to the manufacturer’s instructions under denaturing reaction conditions ...
-
bioRxiv - Cell Biology 2020Quote: ... and deglycosylated with 1000U PNGase F (P0704S, New England Biolabs). Solubilized protein was then digested with 3ug trypsin at 37°C overnight with agitation at 1400rpm before desalting on C18 columns ...
-
bioRxiv - Immunology 2021Quote: ... These two proteins were also treated with PNGase-F (NEB) under the reduced condition to remove N-glycans and loaded on the gel to assess the impact of the glycans on the protein size ...
-
bioRxiv - Cell Biology 2022Quote: ... Digestion with Endo H or PNGase F (NEB, Beverly, MA) were performed according to manufacturer’s suggested protocol ...
-
bioRxiv - Immunology 2022Quote: ... recombinant proteins were treated with PNGase F (New England Biolabs) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... N-glycans were isolated by treatment with PNGase F (NEB) under denaturing conditions followed by solid phase extraction purification ...
-
bioRxiv - Microbiology 2023Quote: ... or deglycosylated by an 1h treatment with PNGase F (NEB) then eluted in 2X Laemmli as previously described.
-
bioRxiv - Molecular Biology 2023Quote: ... 2 μL of peptide N-glycosidase F (New England Biolabs) were added ...
-
bioRxiv - Cancer Biology 2023Quote: ... and deglycosylated with peptideN-glycosidase F (P0704S; New England Biolabs). Samples were then digested sequentially ...
-
bioRxiv - Immunology 2023Quote: ... sample was pre-treated with PNGase F (New England Biolabs) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2023Quote: ... and 2μL PNGase F (500 U/μL, New England Biolabs) was added to deglycosylate the samples over a 2 hour incubation at 37 °C ...
-
bioRxiv - Immunology 2023Quote: ... were subjected to cleavage by PNGase F (New England Biolabs), following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... the N-glycans were released using peptide:N-glycosidase F (NEB, in 50 mM ammonium bicarbonate ...
-
bioRxiv - Cancer Biology 2023Quote: ... lysates were subject to PNGase F treatment (New England Biolabs) to deglycosylate the transporter proteins ...
-
bioRxiv - Cancer Biology 2024Quote: ... or 5,000 U/mL PNGase F (P0704S, New England BioLabs) in growth media for 1 hour at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: ... a chm7Δapq12Δ (CPL1323) strain was transformed with StuI (New England BioLabs linearized pDT30 ...
-
bioRxiv - Molecular Biology 2019Quote: ... coli strains BL21-CodonPlus-RP or Lemo21(DE3, New England Biolabs). Bacterial lysates were prepared as previously described(48 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and cloning was performed in strain NEB 10β (New England Biolabs). A PhlF mutant (PhlFAM ...
-
bioRxiv - Genetics 2020Quote: The mCherry reporter strains were constructed using AgeI-HF (NEB R3552L) linearized reporter plasmids transformed into the SNY250-derived a/Δ wild type strain ...
-
bioRxiv - Immunology 2021Quote: ... Recombinant proteins were induced in SHuffle Express strains (New England Biolabs) upon addition of 1mM IPTG in cultures containing kanamycin 50 μg/mL and incubated with agitation at 37 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... coli strain BL21 (DE3) and subsequently purified with amylose beads (NEB). The RNA affinity purification of mRNA 3’ processing complex was carried out essentially as described before (15) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Escherichia coli strains NEB Turbo and NEB Express (New England BioLabs) were used for cloning ...
-
bioRxiv - Bioengineering 2023Quote: ... coli strains used in this study were DH5α (New England Biolabs) for cloning purposes ...
-
bioRxiv - Biochemistry 2024Quote: ... The protein was expressed using the strain T7express lysY (NEB, C3010I). Expression was induced with 0.4 mM IPTG at OD600 ∼0.6 at 16°C ...
-
bioRxiv - Bioengineering 2024Quote: ... coli strains used in this study were DH5α (New England Biolabs) for cloning ...
-
bioRxiv - Microbiology 2023Quote: ... and strains were obtained from New England Biolabs (NEB, Ipswich, MA) unless otherwise noted ...
-
bioRxiv - Microbiology 2024Quote: ... coli B strain was dephosphorylated with 0.4 U/µL QuickCIP (NEB) in 1 x rCutSmart buffer (NEB ...
-
bioRxiv - Microbiology 2020Quote: ... PNGase F treatment was done according to the manufacturer’s instructions (NEB).
-
bioRxiv - Molecular Biology 2020Quote: ... The first duplicate was deglycosylated by PNGase F (NEB, 1:100) overnight at 37°C in 50 mM ABC prepared in pure H2O18 ...
-
bioRxiv - Cell Biology 2019Quote: ... N-glycans were digested using PNGase-F (New England Biolabs, P0704S) essentially according to manufacturer’s protocol but with a three-hour incubation at 37 °C.
-
bioRxiv - Biochemistry 2020Quote: ... morsitans salivary proteins were treated with PNGase F (New England Biolabs), resolved by SDS-PAGE and transferred onto a PVDF membrane (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: Saliva samples were treated overnight with PNGase F (New England Biolabs) as described above ...
-
bioRxiv - Biochemistry 2020Quote: ... samples were digested overnight at 37°C with PNGase F (NEB). After the N-linked glycans were removed ...
-
bioRxiv - Molecular Biology 2022Quote: ... and were performed using 5-alpha F’ Iq cells (NEB C2992H).
-
bioRxiv - Biochemistry 2020Quote: ... before and after treatment with PNGase F (New England Biolabs, US) were run on a 12.5% polyacrylamide gel under standard conditions ...
-
bioRxiv - Immunology 2022Quote: ... lysates were treated with PNGase F or EndoH (New England Biolabs) following manufacturer’s recommendation.
-
bioRxiv - Molecular Biology 2022Quote: ... followed by 5 µL of PNGase F (New England Biolabs, P0704L). The sample was incubated at 37°C for 3h to allow the de-N-glycosylation to proceed ...
-
bioRxiv - Microbiology 2023Quote: ... 1 µl (500 Units) of PNGase F (New England BioLabs, Inc.) was used to deglycosylate 10 µg of the protein in the presence of 5 mM DTT and mild detergents by incubating at room temperature for 1 hour according to manufacturer’s recommendations.
-
bioRxiv - Immunology 2021Quote: ... The kRSV-DB1-QUAD plasmid and spike insert were digested with the enzymes AatII and SalI (NEB) and ligated with T4 DNA ligase (NEB ...
-
bioRxiv - Biophysics 2021Quote: ... Complexes were prepared by incubating spike ectodomain preparations with either ACE2 (residues 18–615, New England Biolabs), VH ab8 ...
-
bioRxiv - Molecular Biology 2022Quote: ... SARS-CoV-2 Spike variants were generated by site-directed mutagenesis with Q5 polymerase (New England Biolabs) and confirmed by Sanger sequencing ...