Labshake search
Citations for New England Biolabs :
201 - 250 of 642 citations for Rubella Spike Glycoprotein E1 strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... Escherichia coli strain NiCo21(DE3) (New England Biolabs) was transformed with the pLysS plasmid (Novagen ...
-
bioRxiv - Microbiology 2019Quote: ... The strain was further transfected with ScaI (NEB) linearized pBAG1-mNeonGreen ...
-
bioRxiv - Biochemistry 2021Quote: ... coli strain NiCo21(DE3) (New England Biolabs, NEB) transformed with plasmid pIA900 (31 ...
-
bioRxiv - Biochemistry 2021Quote: ... coli strain NiCo21(DE3) (New England Biolabs, NEB) transformed with plasmid pIA900 (31 ...
-
bioRxiv - Biochemistry 2020Quote: Escherichia coli strain BL21 (DE3) (New England Biolabs) chemically competent was transformed by heat-shock with the recombinant plasmid pET28b-AmDODA to express the C-terminal His-tagged AmDODA ...
-
bioRxiv - Plant Biology 2021Quote: The Escherichia coli strain Dh5α (New England Biolabs) was used for cloning purposes ...
-
bioRxiv - Synthetic Biology 2022Quote: ... coli strains DH5αZ1 and NEB10β (New England Biolabs). The different biosensors were grown in LB media with corresponding antibiotics (kanamycin 25 μg/mL or chloramphenicol 25 μg/mL) ...
-
bioRxiv - Cell Biology 2022Quote: ... the bacterial strain NEB10 (New England Biolabs #C3019H) or NEB stable (New England Biolabs #C3040H ...
-
bioRxiv - Microbiology 2021Quote: ... T7 SHuffle (C3026, E. coli K strain, NEB) and Nico (λDE3 ...
-
bioRxiv - Biochemistry 2022Quote: The Escherichia coli strain Dh5α (New England Biolabs) was used for cloning purposes ...
-
bioRxiv - Molecular Biology 2023Quote: ... coli strain NEB 10-beta (NEB Cat# C3019) was used for construction of plasmids by ligation-less Gibson assembly (Fu et al ...
-
bioRxiv - Cancer Biology 2023Quote: ... coli strain T7 Express Iq (NEB, Cat. # C2833H) was optimized to yield ∼100 mg/L of recombinant ORF1p-His6 (346 aa ...
-
bioRxiv - Microbiology 2023Quote: ... coli strain NEB 5-alpha (New England Biolabs) (Table S5).
-
bioRxiv - Microbiology 2023Quote: ... coli strain D10-beta (New England Biolabs Inc.) was used in the entire cloning process ...
-
bioRxiv - Neuroscience 2023Quote: ... coli strain NEB 10-beta (New England Biolabs) was used for all cloning ...
-
bioRxiv - Cell Biology 2023Quote: ... coli BL21(DE3) strain (C2527I, New England Biolabs) and grown in M9 medium for 15N labeling ...
-
bioRxiv - Biophysics 2024Quote: ... coli BL21(DE3) strain (NEB, Ipswich, MA, USA). The transformants were grown aerobically in LB medium supplemented with kanamycin at 37 C° and 225 rpm overnight (seed culture) ...
-
bioRxiv - Cell Biology 2021Quote: ... 20µg of protein lysates and media samples were incubated with glycoprotein denaturation buffer at 95°C for 10 min and treated with 0.3µl Endo H (NEB; Catalog no #P0702) enzyme in glyco-buffer 3 for one hour at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... Lysates were first denatured with Glycoprotein Denaturing Buffer at 65°C for 15 min and then treated with Endoglycosidase H (Endo H) (New England Biolabs, #P0702S) or Peptide-N-Glycosidase F (PNGase F ...
-
bioRxiv - Biochemistry 2020Quote: 20 μg of protein from SK-RC-39 cells were treated with N-glycosidase F (PNGase F) and Endoglycosidase H (Endo H) (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: 30 to 50 μg proteins were digested for 90 min at 37°C with endoglycosidase-H (Endo-H; P0702L) or endoglycosidase-F (Endo-F; P0705S) as recommended by the manufacturer (New England Biolabs).
-
bioRxiv - Plant Biology 2020Quote: ... Proteins with or without PNGase F (NEB, USA) digestion were mixed with the loading buffer (250Mm Tris-HCl PH 6.8 ...
-
bioRxiv - Biochemistry 2020Quote: ... 1 μl of PNGase F (non-reducing, NEB) was added to remove protein N-glycosylations and the sample was incubated at 50°C for 10 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... PNGase F (P0704, NEB, 1,000 units/µg protein) was used to remove the N-linked glycosylation in purified ORF8 protein ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 8U PNGase F (P0704L, NEB; P7367, Sigma) were used ...
-
bioRxiv - Microbiology 2021Quote: ... treated or not treated with PNGase F (BioLabs) for 2 h at 37°C ...
-
bioRxiv - Biochemistry 2021Quote: ... One microliter of PNGase F (New England Biolabs) was added and the deglycosylation reaction proceeded during two hours at 37 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... One microliter of PNGase F (New England Biolabs) was added and the deglycosylation reaction proceeded during two hours at 37 °C ...
-
bioRxiv - Biophysics 2020Quote: ... Eluted protein was deglycosylated with PNGase F (NEB) by incubating 5 units of the enzyme per 1 μg of protein for 2 h at 37 °C under gentle agitation ...
-
bioRxiv - Cancer Biology 2020Quote: ... was performed using PNGase F (New England Biolabs) without a heat-inactivation step ...
-
bioRxiv - Biochemistry 2020Quote: ... 1 μL of PNGase F (NEB Cat # P0704S) was added to the reaction and the digestion allowed to proceed at 37° C for 1 hour ...
-
bioRxiv - Neuroscience 2020Quote: ... 2 μL PNGase F (500U/μL) (NEB # P0704) was added to the elution buffer and the samples were incubated for 3 h at 37 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... E.coli ECOR47 or E.coli dh5a F’ (NEB, C2992). For protein expression and purification ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... or PNGase F (Cat.#P0708L; New England Biolabs). For Endo Hf digestion ...
-
bioRxiv - Microbiology 2024Quote: ... and the PNGase F assay (New England Biolabs) was completed following the manufacturer’s instructions using 15 µg of protein ...
-
bioRxiv - Biochemistry 2022Quote: ... Deglycosylation was performed using Peptide-N-Glycosidase F (PNGase F) at 37 °C for one hour according to the supplier’s protocol (New England Biolabs, Hitchin, UK). Enzyme purity and molecular weight were estimated by 12 % SDS-PAGE using mini-PROTEAN 3 system (BioRad ...
-
bioRxiv - Biochemistry 2022Quote: ... Deglycosylation was performed using Peptide-N-Glycosidase F (PNGase F) at 37 °C for one hour according to the supplier’s protocol (New England Biolabs, Hitchin, UK). Enzyme purity and molecular weight were estimated using a 12% SDS-PAGE and mini-PROTEAN 3 system (BioRad ...
-
bioRxiv - Biophysics 2019Quote: ... the Escherichia coli BL21 (DE3) strain (NewEngland Biolabs, USA) was transformed with this vector ...
-
bioRxiv - Microbiology 2022Quote: ... coli strain DH5α (New England Biolabs, Ipswich, MA, USA) was used for sub-cloning procedures and plasmid purification ...
-
bioRxiv - Microbiology 2022Quote: ... coli strain DH5α was obtained from NEB (cat. C2987I). E ...
-
bioRxiv - Immunology 2022Quote: ... strain Shuffle pLys Y (New England Biolabs, cat: C3030J) compatible with pET vectors (DE3) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... coli strains: NEB 5-alpha (NEB C2987, not authenticated), BL21-AI (ThermoFisher C607003 ...
-
bioRxiv - Biochemistry 2020Quote: ... coli strain T7 Express (New England Biolabs, Ipswich, USA) at 37 °C in 5 mL LB medium containing 25 μg mL−1 kanamycin by induction with 1 mM IPTG for 3 hours ...
-
bioRxiv - Biochemistry 2020Quote: ... coli strain T7 Express (New England Biolabs, Ipswich, USA) as described in section “Proteolytic inhibition assays” ...
-
bioRxiv - Bioengineering 2019Quote: ... coli strain NEB5α (New England Biolabs, Ipswich, MA, USA) was grown in Luria−Bertani (LB ...
-
bioRxiv - Biochemistry 2020Quote: ... E.coli BL21 (DE3) strain (New England BioLabs, Cat#C2527) was used for overexpression of xlGeminin and the N-terminal residues (1-170 ...
-
bioRxiv - Biochemistry 2020Quote: ... E.coli T7 Express strain (New England BioLabs, Cat#C2566) was used for overexpression of LacI and M.HpaII ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The NEB® DH10B strain (New England Biolabs, #C3019H) was used to assemble and amplify the constructs ...
-
bioRxiv - Biochemistry 2021Quote: ... coli strain NEB Express C2566 cells (New England Biolabs) carrying the pRare2 ...
-
bioRxiv - Biochemistry 2019Quote: Plasmids were used to transformed E.coli strain C2566 (NEB) protease-deficient and carrying pRARE plasmid (Novagen) ...