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Citations for New England Biolabs :
351 - 400 of 642 citations for Rubella Spike Glycoprotein E1 strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Spike variants with single-site residue substitutions were generated using Q5® High-fidelity DNA polymerase (NEB)-based site-directed mutagenesis.
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bioRxiv - Microbiology 2021Quote: ... Clones were selected and maintained in E.coli strain NEB5-alpha (NEB, C2987I). All constructs were verified by Sanger sequencing (Eurofins) ...
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bioRxiv - Microbiology 2019Quote: ... Bacillus subtilis 3610 (33) and Escherichia coli strains NEB10β (New England Biolabs) and WM3064 were cultured aerobically on LB agar or in LB broth ...
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bioRxiv - Genetics 2019Quote: ... coli strains NEB10®-beta (New England BioLabs Inc., Ipswich, MA, USA), and XL1-blue (Agilent Technologies ...
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bioRxiv - Microbiology 2019Quote: Bradyzoite reporter strain parasites were transfected with 50 μg of AseI (NEB) linearized pU6-SAG1-DHFR (AddGene #80322) ...
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bioRxiv - Microbiology 2019Quote: ... Bradyzoite reporter strain parasites were transfected with 50 μg of AseI (NEB) linearized pU6-BFD1-DHFR ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain T7 Express lysY/Iq (New England Biolabs, Ipswich, MA, USA) and purified by Ni- ...
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bioRxiv - Bioengineering 2021Quote: Escherichia coli strains DH5α [15] and ER2523 (New England Biolabs, Ltd., UK) were grown in Luria Bertani (LB ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli strain K12 (NEB Turbo Competent E. coli, New England Biolabs #C2984) was used for routine cloning ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli strain DH10-β cells (45 μL each; C3019I, New England Biolabs) thawed on ice for 10 minutes and mixed gently by tapping ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli strain K12 (NEB Turbo Competent E. coli, New England Biolabs #C2984) was used for routine cloning ...
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bioRxiv - Microbiology 2021Quote: All plasmid protection assays were done in Escherichia coli (strain: NEB Turbo). As described previously12 ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid was transformed into Escherichia coli expression strain BL2lλDE3 (New England Biolabs) for expression ...
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bioRxiv - Biochemistry 2020Quote: Proteins have been expressed in Escherichia coli strain BL21 (DE3) (NEB # C2527). In brief ...
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bioRxiv - Microbiology 2023Quote: The strain Escherichia coli NEB 5-alpha (New England Biolabs, NEB C2987H) was used as a cloning strain ...
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bioRxiv - Microbiology 2023Quote: The strain Escherichia coli NEB 5-alpha (New England Biolabs, NEB C2987H) was used as a cloning strain ...
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bioRxiv - Biochemistry 2023Quote: ... coli strains Nico21 (DE3) pLysS or BL21-codon plus (New England BioLabs) were used for overexpression ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: For analysis of protein N-glycosylation PNGase F (P0704S, New England Biolabs) was used and the assay was performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... S protein were first incubated with either 1000U/mL PNGase F (NEB) or deactivated PNGase F at 37 °C for 14 h ...
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bioRxiv - Biochemistry 2021Quote: PNGase F treatment was conducted as per manufacturer’s protocol (New England Biolabs). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... protein extracts were treated with PNGase F according to manufactures instructions (NEB).
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bioRxiv - Immunology 2020Quote: The fusion protein was treated with glycerol free PNGase F (NEB, P0705S) in non-denaturing reaction conditions ...
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bioRxiv - Biochemistry 2021Quote: ... To release N-glycans 2 μl of PNGase F (New England Biolabs) was added and the samples were incubated for 18 h in 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... After 24 h cell lysates were treated with PNGase-F (NEB, P0704L) or water by following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: The N-glycans of ACE2 were removed by PNGase F glycosidase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: The recombinant Lili-Mips were treated with PNGase F (New England Biolabs) to remove the N-linked oligosaccharides ...
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bioRxiv - Biochemistry 2023Quote: Samples were treated first with 250 U PNGase F (New England Biolabs) for 2 hr at 37 °C to condense each protein to a single molecular weight ...
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bioRxiv - Microbiology 2022Quote: ... resulting in a linearized vector into which synthetic variant spike genes can be assembled using Gibson Assembly (NEB). The Gibson Assembly reaction was then transformed into TransforMax™ EPI300™ Electrocompetent E ...
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bioRxiv - Microbiology 2022Quote: ... PCR of a spike gene fragment was performed using Q5 High-Fidelity 2X Master Mix (New England Biolabs) and the following primers ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli K-12 strain NEB DH-10 Beta (New England Biolabs, MA, USA) unless stated otherwise ...
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bioRxiv - Microbiology 2022Quote: ... All cloning and strains were performed in dh10b genotype cells (NEB, Intact Genomics).
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bioRxiv - Bioengineering 2022Quote: Escherichia coli (E. coli) strain NEB 5-α (New England Biolabs, Hertfordshire, UK) was used for generation of genetic constructs ...
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bioRxiv - Microbiology 2020Quote: ... was used for strain D236 and the nicking enzyme combination Nt.BspQI/Nb.BbvCI (NEB) was used for strains D800 ...
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bioRxiv - Immunology 2020Quote: ... CD45 catalytic domain DNA constructs were transformed into the BL21 (DE3) strain (NEB) Escherichia coli and plated on LB agar with ampicillin (100 mg/ml) ...
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bioRxiv - Bioengineering 2021Quote: This study used the Escherichia coli strains NEB10β (New England Biolabs, Ipswich, MA), Nissle 1917 as well as all strains listed in Table 1 ...
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bioRxiv - Microbiology 2022Quote: pWR1566 was purified from laboratory E.coli strain NEB 10-beta (New England Biolabs) and used to transform V.cholerae strain WR2700 using electroporation ...
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bioRxiv - Synthetic Biology 2023Quote: ... Discrete strains were assembled using the HiFi DNA Assembly kit (NEB, cat #E2621) from IDT gBlocks ...
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bioRxiv - Microbiology 2023Quote: ... aureus strains were performed using Monarch® Total RNA Miniprep Kit (NEB, UK) and SensiFAST cDNA Synthesis Kit (Bioline ...
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bioRxiv - Developmental Biology 2023Quote: ... Freshly laid wild-type TU-strain embryos were injected with SpCas9 protein (NEB) mixed with gRNAs (~300 ng/ul) ...
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bioRxiv - Neuroscience 2021Quote: ... Glycosylation was removed from denatured extracted lysates using PNGase F (NEB, Cat# P0704S) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... N-glycans were enzymatically removed from the underlying peptides with PNGase F (NEB) overnight at 37 °C ...
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bioRxiv - Biophysics 2019Quote: Deglycosylation experiments in reducing conditions were performed with PNGase F (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... and proteins were de-glycosylated with PNGase F (500,000 U/ml, New England Biolabs) overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ~1 μg of tsetse saliva was treated with PNGase F (New England Biolabs). Samples were run on a 12.5% polyacrylamide gel ...
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bioRxiv - Biochemistry 2020Quote: The reserved aliquots were incubated with 500 units PNGase F (New England Biolabs) for every 20 μg of glycoprotein overnight at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: Three microgram of full-length S protein were treated with PNGase F (NEB) for N-glycan release according to the manufacture’s instruction ...
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bioRxiv - Microbiology 2020Quote: Purified sE2 monomers were treated with PNGase F or EndoH (New England BioLabs) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... before and after deglycosylation with endoglycosidase PNGase F (New England Biolabs, Massachusetts, US). Gel was stained using InstantBlue Protein stain (Expedeon ...
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bioRxiv - Cell Biology 2021Quote: ... solubilized proteins were treated with Endo H and PNGase F (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: Washed RBCS were deglycosylated by incubation with 10% PNGase F (New England Biolabs) at 37°C for 2 hours ...