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Citations for New England Biolabs :
4001 - 4050 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... 260 to 300bp mRNA (CDS or UTR) sequences of enzymes were amplified by PCR using Q5® High-Fidelity DNA Polymerase (New England Biolabs) with primers listed in Supplementary Table S6and cut by BamHI and SalI (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... backbone (containing Blasticidin resistance gene for the selection of transduced cells) and mCherry gene were PCR amplified using Q5® Hot Start High-Fidelity DNA Polymerase (New England Biolabs), remnant parent templates were digested using DpnI (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2024Quote: ... All PCR reactions were carried out in 30 μL reactions with 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers ...
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bioRxiv - Genomics 2024Quote: ... 10 µL of tagmented DNA was PCR amplified for 14 cycles in a 50 µL reaction volume using NEBNext High-Fidelity 2X PCR Master Mix (NEB # M0541S) and Nextera primers ...
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bioRxiv - Microbiology 2024Quote: ... DNA sequences were amplified and amended with ≥18 bp homology to their destination vectors using Q5 Hot Start High Fidelity Master Mix (NEB, M0494L). Vectors were constructed using Gibson Assembly with HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... One microliter of the LEAP reaction then was amplified in a 25 μL PCR reaction using Q5 Hot Start High-Fidelity 2X Master Mix (NEB, M0494S) with primers specific for sequences within the SV40 promoter of the neoTet and mneoI reporter cassettes (NeoSV40_fwd ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 μL of the diluted cDNA reaction was used in PCR reactions (25 μL) using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB, M0494S) with NeoSV40_fwd and 3′LEAPOuter primers ...
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bioRxiv - Biophysics 2024Quote: ... PCR was performed according to manufacturer’s instructions for the Q5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs, USA). After 30 s at 98 ℃ for initial denaturation ...
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bioRxiv - Genomics 2024Quote: ... the Illumina flow cell adapters were added through a 15-cycle PCR using NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs, M0541L), the forward primer pLSmP-ass-i741 and reverse primer pLSmP-ass-gfp ...
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bioRxiv - Genomics 2024Quote: ... The purified fragment underwent a second round of 12-cycle PCR using NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs, M0541L), the forward primer 5BC-AG-f02 and the reverse primer 5BC-AG-r02 ...
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bioRxiv - Genomics 2024Quote: ... A synthesized TWIST oligo pool with 300 bp long elements (270 bp insert + 2*15 bp PCR handles) was amplified by 12-cycle PCR using NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs, M0541L), the forward primer 5BC-AG-f01 and reverse primer 5BC-AG-r01 were used to add the minimal promoter ...
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bioRxiv - Zoology 2024Quote: ... we generated a template for dsRNA synthesis by amplifying targeted coding sequences from cDNA using gene-specific primers with T7 overhangs and Q5 High-Fidelity DNA Polymerase (NEB, M0491S). Two rounds of PCR reactions (each with 35 cycles ...
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bioRxiv - Neuroscience 2021Quote: ... T7 HiScribe kit (NEB) was used to transcribe the sgRNA DNA template with the appended T7 promoter ...
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bioRxiv - Cell Biology 2020Quote: ... and Illumina sequencing adaptor ligation (New England BioLabs, E6000B-10). Libraries were then indexed and PCR amplified (10 cycles ...
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bioRxiv - Genetics 2021Quote: ... and 10 U polynucleotide kinase (New England Biolabs, Beverly, MA) as instructed by the manufacturer ...
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bioRxiv - Genomics 2020Quote: ... 10 μL 10x T4 DNA Ligase Reaction Buffer (NEB B0202S), 0.5 μL 200x BSA (NEB ...
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bioRxiv - Genomics 2021Quote: Lambda exonuclease: 10 U λ exonuclease (New England Biolabs, M0262) in 60 μl 1×λ exonuclease reaction buffer was added and incubated at 37°C for 30 minutes.
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bioRxiv - Genomics 2020Quote: ... 1 μl (10 U/μl) of T4 polynucleotide kinase (NEB) and 1 μl of 10 mM dNTPs in a final volume of 50 μl at 24°C for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... and 10 mM vanadyl-ribonucleoside complex (S1402S, New England BioLabs) with probes at final concentration of 1 ng/μl ...
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bioRxiv - Genomics 2019Quote: ... DpnII buffer and DpnII (10 U, New England Biolabs #R0543L) were added in a total volume of 50 µl and incubated at 37 °C for 1 h ...
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bioRxiv - Genetics 2021Quote: ... 10 uL of 10U/uL AluI restriction enzyme (NEB R0137S) was added to each sample ...
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bioRxiv - Genomics 2021Quote: ... 120 μL 10×NEB T4 DNA ligase buffer (NEB B0202), 100 μL of 10% Triton X-100 ...
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bioRxiv - Genetics 2020Quote: ... we added 10 µL of Shrimp Alkaline Phosphatase (NEB #M0371L) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... and 10 mM Ribonucleoside-Vanadyl Complex (NEB, Ipswich, MA, USA) on ice ...
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bioRxiv - Genomics 2020Quote: ... 1 µl Calf Intestinal alkaline Phosphatase (10 U; CIP, NEB) or quick CIP (10 U ...
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bioRxiv - Biochemistry 2020Quote: ... and 50 µL of 10 mM dNTPs (New England BioLabs), 100 µL 0.1 M DTT ...
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bioRxiv - Cell Biology 2021Quote: ... cells were initially incubated with SNAPcell block (10 µM; NEB) diluted in culture media for 20 min ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 4 μl 10× T4 RNA ligase buffer (New England Biolabs), 4 μl T4 RNA ligase ...
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bioRxiv - Systems Biology 2020Quote: ... 22 μL of 10 mM/each of four dNTPs (NEB), 5.5 μL of TSO primer (100 μM) ...
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bioRxiv - Microbiology 2021Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 50,000 variants per duplicate library ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 mM adenosine triphosphate (New England Biolabs, United States) was added to the 17 μl solution from the first step ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain NEB 10-β competent cells (New England Biolabs) and plated on LB agar containing 50 μg/mL kanamycin ...
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bioRxiv - Genomics 2022Quote: ... The product was transformed into 10-beta Electrocompetent E.coli (NEB), by electroporation with 2.0kV ...
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bioRxiv - Microbiology 2022Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 100,000 variants per duplicate library ...
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bioRxiv - Molecular Biology 2020Quote: ... 5% DMSO and 10 units of λ exonuclease (NEB, M0262S) at 37°C for 30 min ...
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bioRxiv - Biochemistry 2021Quote: 10 ug of protein extract were digested with PNGaseF (NEB) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 µl of 2X Phusion Master Mix (NEB, Cat#M0531L), and 3.6 µl H2O (20 µl reaction) ...
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bioRxiv - Genomics 2022Quote: ... Each reaction contained 10 µL of Q5 Reaction Mix (NEB), 10 µL of Q5 GC Enhancer Buffer (NEB) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 10 μl of 5 U/μl Klenow (NEB M0210)) and incubating 1h at 37ºC with rotation ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 2.5 μl of T4 PNK (10 U/μl, NEB M0201) and 0.5 μl of Klenow DNA polymerase (5 U/μl NEB M0210) ...
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bioRxiv - Microbiology 2022Quote: ... with the addition of 10 mg BSA (New England BioLabs). We added 0.1 femtomole 515F and 806R which do not have the barcodes and adapters to overcome initial primer binding inhibition because the majority of the primers do not match the template priming site ...
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bioRxiv - Microbiology 2022Quote: ... 2 μl 10× T4 RNA ligase reaction buffer (NEB, M0242), 10 μl 50% PEG 8000 (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μl Thermo Pol Buffer (New England Biolabs, Ipswich, MA), 2 μl of 10 mM dNTPs (New England Biolabs) ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μl of Sulfolobus DNA polymerase IV (New England Biolabs) and nuclease-free water to 1 ml ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 μl of 10X DNase I Buffer (New England Biolabs), and 2 μl of DNase I (2 U/μl ...
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bioRxiv - Plant Biology 2020Quote: ... 10 μl MNase (2,000,000 gels units/ml, NEB, Ipswich, Massachusetts) with appropriate units was added to 30 μl sample and incubated at 37°C for 15 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl T4 PNK (NEB, cat#M0201S, 10 U/μl), 1.5 μl T4 DNA Polymerase (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of T7 Endonuclease I (NEB, 10 units/μl) was added and incubated at 37 °C for 15 min.
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bioRxiv - Synthetic Biology 2021Quote: ... coli Poly(A) Polymerase (10 units; M0276S, New England Biolabs) with nuclease-free water (29 μL) ...
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bioRxiv - Molecular Biology 2021Quote: ... DpnII buffer and DpnII (10 U, New England Biolabs #R0543L) were added in a total volume of 50 μl and incubated at 37 °C for 1 h ...