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Citations for New England Biolabs :
3901 - 3950 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... a SYBR green qPCR side reaction with 1/10 of the preamplified solution to determine the extra cycle number and a final PCR with individually adapted cycle numbers to avoid duplicates and ensure similar final concentrations using NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs, M0541S) and Nextera Index Kit (Illumina ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Illumina adapters with unique i7 indices were added via 12-cycle PCR with Phusion high-fidelity DNA polymerase (New England Biolabs, Inc.). Three libraries were pooled per lane and sequenced single-end on an Illumina HiSeq 4000 platform (100bp read length ...
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bioRxiv - Immunology 2022Quote: ... 100 µl volume) using primers described in Table S3C and Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Beverly, MA). This provides approximately 300X coverage for sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... gene with stop codon was amplified from wildtype Col-0 cDNA by Phusion High-Fidelity DNA Polymerase (New England Biolabs, #M0530L) and cloned into the pENTR plasmid (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... PCR assays were performed with the strain-specific multiplex primers from (46) and Q5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs) in 20 µl reactions ...
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bioRxiv - Microbiology 2022Quote: ... The standard curve was created from PCR product generated using the inovirus primer set (above) and a Phusion® High-Fidelity DNA Polymerase (NEB) using extracted DNA from each strain as a template ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL of the heated solution was then used as template for PCR using the Q5® High-Fidelity DNA Polymerase (NEB), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... with a terminal elongation step for 4 min at 72°C employing a proofreading Taq polymerase (Q5 High-Fidelity DNA polymerase, New England Biolabs, Germany). After verification of the amplicon sequence ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA fragments to be assembled were amplified by PCR using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532S), purified with gel electrophoresis and Zymoclean Gel DNA Recovery Kit (Zymo Research ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR reactions were performed with a final primer concentration of 1 mM using Q5® High-Fidelity 2X Master Mix (NEB). Equal volumes of X and Y PCR reactions were then combined ...
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bioRxiv - Plant Biology 2022Quote: ... including the stop codon and (2) the CDS and native promoter region 1024 bp upstream using Phusion High Fidelity DNA polymerase (NEB, USA) and TA-ligated into the entry vector pCR8/GW/TOPO (Thermo ...
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bioRxiv - Neuroscience 2023Quote: ... PCRs to confirm targeted and untargeted SMN2 loci were performed for each of the cell lines with primer pairs shown in table 1 using High-Fidelity 2X PCR Master Mix (NEB, M0541L) and Thermal Cycler C1000 Touch (Bio-RAD) ...
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bioRxiv - Microbiology 2022Quote: The coding region for OspA with its native intergenic promotor was amplified by PCR with Q5 thermostable high-fidelity polymerase (NEB, M0491) from B31-e2 genomic DNA with primers 35 and 36 (Table 1 ...
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bioRxiv - Neuroscience 2023Quote: Variants were introduced into NaV1.2 by site-directed mutagenesis using Q5 2X high-fidelity DNA polymerase Master Mix (New England Biolabs, Ipswich, MA) as previously described (15) ...
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bioRxiv - Microbiology 2022Quote: ... genes of interest were amplified from phage or bacterial genomic DNA using Q5 Hot Start High Fidelity Master Mix (NEB, M0494L), or synthesized as GeneFragments (Genewiz ...
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bioRxiv - Genomics 2022Quote: ... 1 μg of gDNA was amplified in a 200 μl reaction using Q5® High-Fidelity 2X Master Mix (NEB #M0491). PCR master mix (100 μl Q5 ...
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bioRxiv - Microbiology 2022Quote: ... Primers were designed to amplify a 500 or 1000 base pair amplicon of the targeted locus using either Q5 High-Fidelity DNA Polymerase (NEB; M0491S) or Platinum Taq DNA Polymerase High Fidelity (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... the full region including the HrpL box promoter was PCR-amplified using primers (Supplementary Table S2) and Q5 High-Fidelity DNA Polymerase (NEB, USA). The resulting PCR fragment was gel-purified and was blunt-end-ligated into the Eco53kI (NEB ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cDNAs were subcloned into vectors through conventional ligation with Ligation high Ver.2 (Toyobo, Japan) or NEBuilder HiFi DNA Assembly (New England Biolabs, MA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... A total of 1µg of the purified PCR product was used for in vitro transcription using the HiScribe T7 High Yield RNA synthesis (NEB E2040S), as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting products were pre-amplified in a 100 μL reaction using 10 cycles with 4 μL pre-amp primers (10x Genomics PN 20002714) and 50 μL of NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541S). Reactions were cleaned with 1.6X SPRI and eluted in 40 μL EB ...
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bioRxiv - Systems Biology 2024Quote: ... The resulting full-length cDNA was gel-purified and ligated to the 5′ adaptor OWG920 with High Concentration T4 RNA Ligase (NEB M0437M) overnight on a shaking thermomixer ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed in a 50 μl volume containing Q5® Hot Start High-Fidelity DNA Polymerase (New England Biolabs). Quantitative analysis of the purified libraries was performed using Qubit 3.0 Fluorometer and Qubit® dsDNA HS Assay kit (InvitrogenTM) ...
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bioRxiv - Microbiology 2024Quote: nfsA and nfsB mutants were constructed by gene inactivation using the pKNOCK suicide plasmid (22) The DNA fragments were amplified with Phusion high-fidelity DNA polymerase (NEB, UK) from E ...
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bioRxiv - Microbiology 2024Quote: ... The full-length coding region with introns spliced out of PIGJ was amplified using the Q5 high fidelity polymerase (NEB, M0491) from a CEP cDNA library preparation using primers designed to contain 19 bp homology with the 3’ end of the PIGJ promoter sequence and 25 bp homology with end of the digested pLIC-HA plasmid that contains and provides a 3x HA tag ...
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bioRxiv - Neuroscience 2024Quote: ... the resulting product was purified via column purification and underwent further amplification and conversion to RNA by a high-yield in-vitro transcription reaction using T7 polymerase (NEB, E2040S). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... hcp and tssB flanking regions were PCR-amplified from CFBP13503 with the Phusion High-Fidelity DNA polymerase (New England Biolabs, France), and the primer pairs listed in Table S2 ...
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bioRxiv - Biochemistry 2024Quote: ... The Ni-elution was collected directly on a pre-equilibrated amylose column (amylose high flow resin, New England Biolabs, Ipswich, Massachusetts). Amylose column was washed with 5 column volume cold wash buffer before fractionated elution in a buffer containing 25 mM Hepes pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were amplified for N-1 cycles (being N the optimum Cq determined by qPCR reaction) using NEBNext High-Fidelity Polymerase (New England Biolabs, M0541). Libraries were purified with Sera-Mag Select Beads (GE Healthcare ...
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bioRxiv - Genetics 2024Quote: ... Each of the four cDNA products was then used as a template in a PCR with the same primers and Phusion® High Fidelity DNA Polymerase (New England Biolabs). All primer pairs were located within the 5’- and 3’-UTRs of the transcripts so that they amplified the entire protein coding sequence of the respective genes (Figure 1 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we first confirmed the presence of mutations in the gene coding for pb1 in evolved phage populations by sequencing (Sanger; Eurofins, Nantes, France) the amplicon obtained from PCR using Q5 high-fidelity DNA polymerase (#M0491; New England Biolabs NEB) and the primers Salten-pb1-3183-F / Salten-pb1-4136-R primers (TableS1) ...
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bioRxiv - Genomics 2024Quote: Two microliters of the cDNA were used for PCR amplification employing Taq polymerase (Genaxxon Bioscience) for HEK293T-derived samples and Q5® High-Fidelity DNA Polymerase (New England Biolabs). Primers are listed in Supplementary Table 5 ...
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bioRxiv - Genomics 2024Quote: ... gDNA was first amplified with primers JLSPr141–144 and JLSPr165–168+171+172 using Q5 High-Fidelity DNA Polymerase (NEB #M0491) for 20 cycles with an annealing temperature of 65°C ...
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bioRxiv - Genetics 2024Quote: Wild-type minigene plasmids were prepared using target exons and variable flanking intronic sequences of the DMD gene amplified from a control genomic DNA using Q5 High-Fidelity DNA Polymerase (NEB, USA). The primers that were used are described in Table S1 ...
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bioRxiv - Genomics 2024Quote: ... Amplicons were then barcoded for NGS by combining 2 µL of amplicon from the previous PCR with 1X Q5 High-Fidelity Master Mix (New England Biolabs M0492) and 500 nM forward and reverse indexing primers (Integrated DNA Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... the MsbA gene (from Escherichia coli genomic DNA) was amplified by polymerase chain reaction (PCR) using Q5 High-Fidelity DNA Polymerase (New England Biolabs, NEB) and subcloned into a modified pCDF-1b plasmid (Novagen ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA fragments for construction of the plasmids were obtained through either high-fidelity amplification using Q5 DNA Polymerase (NEB, USA) or restriction enzyme digestion ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by PCR amplification of the BC-sgRNA1-sgRNA2 region from 32 μg of bulk lung genomic DNA using Q5 Ultra II High-Fidelity 2× Master Mix (New England Biolabs, M0494X). Unique dual-indexed primers were used to amplify each sample followed by purification using Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Bioengineering 2024Quote: ... Target cDNA were then amplified using one primer that annealed specifically to the target RNA and a second primer that annealed specifically to the rcRNA (primers 45/46 for dmd and primers 47/48 for lmna) with Q5® High-Fidelity DNA Polymerase (New England Biolabs) for 35-cycles.
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bioRxiv - Bioengineering 2024Quote: ... The splitGFP plasmid was amplified using primers 53 and 54 to install 3’ stop codon and primers 55 and 56 to install the 5’ stop codon using Q5® High-Fidelity DNA Polymerase (New England Biolabs) for 35-cycles ...
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bioRxiv - Bioengineering 2024Quote: ... Target cDNA were then amplified using primers that span the trans-splice junction (primers 41/42 for dmd and primers 43/44 for lmna) and predicted mutation with Q5® High-Fidelity DNA Polymerase (New England Biolabs) for 25-cycles ...
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bioRxiv - Bioengineering 2024Quote: ... Primers containing the spacer sequence and homology to the rcRNA plasmid backbone were annealed using Q5® High-Fidelity DNA Polymerase (New England Biolabs) for 35-cycles ...
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bioRxiv - Bioengineering 2024Quote: ... either half of the EGFP open reading frame was amplified from pLHA-TR-EF1α-Hipk3-Polio-SplitGFP45 with primers 49 and 50 (first half of EGFP ORF) and primers 51 and 52 (second half of EGFP ORF) using Q5® High-Fidelity DNA Polymerase (New England Biolabs) for 35-cycles ...
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bioRxiv - Microbiology 2023Quote: ... Each sample was amplified in triplicate 25 μl reactions consisting of primers at final concentrations of 0.2 μM and 1X PCR reagent (Q5® Hot Start High-Fidelity 2X Master Mix, New England BioLabs, UK). Triplicate PCR reactions for each sample were then pooled and purified using Agencourt® AMPure® XP beads (#A63881 ...
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bioRxiv - Microbiology 2023Quote: ... The AvcI DNA template for in vitro transcription was PCR amplified from pAvcI using Q5 High-Fidelity DNA Polymerase (NEB™). To incorporate the T7 promoter into the final AvcI DNA template ...
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bioRxiv - Immunology 2023Quote: ... 3000 ng of high molecular weight DNA was prepared by blocking available DNA ends with calf intestinal phosphatase (NEB Cat #M0525). DNA and dA-tails on all available DNA ends were cleaved by RNPs and Taq polymerase (New England Biosciences Cat #M0273 ...
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bioRxiv - Systems Biology 2022Quote: ... The Ni-elution was collected directly on a pre-equilibrated amylose column (amylose high flow resin, New England Biolabs, Ipswich, Massachusetts). Amylose column was washed with 5 column volumes of cold wash buffer then eluted in a buffer containing 25 mM Hepes pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... pCS2+ backbones containing ACVR1 or an FOP-ACVR1 mutant were PCR amplified with the mutant primer using Phusion high fidelity polymerase (NEB, M0530). Mutated plasmids were transformed into Top10 chemically competent cells and DNA from individual colonies was submitted for Sanger sequencing ...
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bioRxiv - Systems Biology 2022Quote: ... PCRs for screens with the vTR and CoV libraries were performed using NEBNext High-Fidelity 2X PCR Master Mix (NEB #M0541L) with 33 cycles ...
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bioRxiv - Genomics 2023Quote: ... DNA fragments were further size-selected by agarose gel elution and PCR amplified for 6 to 8 cycles using Illumina P1 and Index primer pair and Phusion® High-Fidelity PCR Master Mix (New England Biolabs). The final library was purified using Agencourt AMPure XP beads and quality assessed by Agilent Bioanalyzer 2100 (DNA 7500 kit ...