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Citations for New England Biolabs :
3801 - 3850 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... RNA samples were thawed and mixed with an equal volume of 2x RNA Loading Dye (NEB) and loaded onto the gels ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed in 25 μL reaction volumes using 12.5 μL of Taq 2X MasterMix (NEB), 9.5μL water ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcriptase and RT-qPCR reactions were conducted using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, United States). The reaction was performed with 50 ng of total RNA in a total volume of 20 µL ...
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bioRxiv - Cancer Biology 2020Quote: ... were generated using NEBNext Single Cell/Low Input RNA Library Preparation kit (New England Biolabs) and sequenced in a NextSeq500 sequencer SE75bp (Illumina) ...
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bioRxiv - Plant Biology 2021Quote: ... using NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, MA, USA), Iso-Seq Express Oligo Kit ...
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bioRxiv - Developmental Biology 2019Quote: ... Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent’s Bioanalyzer DNA 1000 chip kit ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were prepared using the NEBNext Low Input RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... T4 Ligase and low molecular-weight DNA ladder were obtained from New England Biolabs (NEB). Methyltetrazine DBCO ...
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bioRxiv - Genomics 2023Quote: ... The NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module kit (New England Biolabs) was used to generate cDNA from the extraction ...
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bioRxiv - Systems Biology 2024Quote: ... Low complexity regions of JNK2α2 and ASK1 were deleted using Q5 Site-Directed Mutagenesis (NEB). Oligonucleotides used for cloning and site-directed mutagenesis are listed in the Table S2.
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bioRxiv - Genomics 2019Quote: ... and by qPCR (NEBNext Library Quant, New England Biolabs, LightCycler480, Roche). Furthermore ...
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bioRxiv - Molecular Biology 2019Quote: ... with Luna Universal One-Step RT-qPCR reagents (New England Biolabs). 50 ng total RNA and 250 nM primers were added in each reaction ...
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bioRxiv - Microbiology 2021Quote: ... The Luna Universal One-Step RT-qPCR Kit (New England Biolabs) was used ...
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bioRxiv - Genetics 2022Quote: ... we used the Luna One Step RT-qPCR kit from NEB according to the manufacturer’s instructions at 10μl total volume in 384 well plates ...
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bioRxiv - Molecular Biology 2019Quote: ... Libraries were quantified using qPCR (NEBNext Library Quantification, New England Biolabs) and an Agilent Bioanalyzer and then pooled for sequencing on an Illumina NextSeq 500 ...
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bioRxiv - Systems Biology 2019Quote: ... and the Luna Universal One-Step RT-qPCR Kit (NEB, E3005E) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... or Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Primer sequences used are described in Table S2 ...
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bioRxiv - Microbiology 2022Quote: qPCR was performed on cDNA using Luna (New England BioLabs #M3003) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... by Luna Universal One-Step RT-qPCR Kit (New England BioLabs) according to the manufacturer protocol ...
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bioRxiv - Genomics 2023Quote: ... run and qPCR with NEBNext Library Quant kit (New England Biolabs) respectively ...
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bioRxiv - Microbiology 2023Quote: ... which contained 10 μL Luna Universal qPCR mastermix (New England Biolabs Inc. ...
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bioRxiv - Systems Biology 2023Quote: ... using the Luna Universal One-Step RT-qPCR Kit (NEB, #E3005). No template and genomic DNA controls were included in all experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... Luna® Universal One-Step RT-qPCR Kit (NEB, Ipswich, Massachusetts) was used to quantify the RNA samples per the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2023Quote: ... The Luna Universal One-Step RT-qPCR Kit (New England Biolabs) was used for the detection of viral genomes in the heat-inactivated samples performed through reverse transcription quantitative polymerase chain reaction (RT-qPCR) ...
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bioRxiv - Neuroscience 2024Quote: ... and then quantified by qPCR (NEBNext Library Quant Kit, NEB, E7630). 20 pM of DNA were loaded onto an Illumina MiSeq (v3 ...
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bioRxiv - Cell Biology 2024Quote: ... and young organelles (SNAP-Cell® Oregon Green, NEB), as previously described in the methods section ...
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bioRxiv - Cell Biology 2022Quote: ... All purified DNA (~20 µl) was used for PCR amplification with NEBNext High Fidelity 2x MasterMix (NEB) and optimum cycle number was determined by qPCR ...
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bioRxiv - Cell Biology 2022Quote: Lysates (20 μg) and media immunoprecipitates were diluted to 10 μL in 2X glycoprotein denaturing buffer (NEB), and boiled for 10 min ...
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bioRxiv - Plant Biology 2021Quote: ... DNA fragments were PCR-amplified for 19 cycles using the NEB 2X Taq MasterMix (NEB, MA, USA) and 35 ng of starting DNA in an Eppendorf Mastercycler ep gradient S (Eppendorf ...
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bioRxiv - Microbiology 2021Quote: ... and 10 μl of 2x Quick T4 ligase buffer and 1 μl Quick T4 DNA ligase (NEB) were added and incubated at room temperature overnight ...
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bioRxiv - Genomics 2020Quote: ... 50ng digested px330 was mixed with 1:250 diluted oligo duplex with 2X quick ligation buffer (NEB) and quick ligase (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... Amplicons from mouse embryo lysates were generated using Q5 Hot Start High-Fidelity 2x Mastermix (M0492, NEB) in a 25 μL reaction ...
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bioRxiv - Biophysics 2023Quote: ... 240 pmol of each primer (P1 and P2) and Phusion 2X mastermix with HF buffer (NEB, M0531S). The PCR mix was split into 100 μl aliquots and subjected to 35 cycles of 10 s 98 °C denaturation ...
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bioRxiv - Genomics 2024Quote: ... Illumina TruSeq adapters were ligated to DNA fragments in a reaction containing 2X Quick ligation buffer (NEB), 0.25 μM of adapter and 2 μL of Quick ligase (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... of constructs utilized for plasmid assembly were amplified by NEB Q5 Hot-Start 2X Mastermix according to the manufacturer’s protocol (New England Biolabs, Ipswitch, MA, USA; M0494L). Amplifications of difficult templates (such as the CAG promoter ...
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bioRxiv - Biochemistry 2021Quote: ... The subsequent CRISPR detection was performed by adding 8.75 μL of CRISPR Mix (1X TtCmr Activity Assay Buffer, ~62.5 nM TtCmr complex, 500 nM TTHB144, 2 μL NTP Buffer Mix (NEB #E2050), 25 U Hi-T7 RNA polymerase (NEB #M0658) ...
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bioRxiv - Biochemistry 2022Quote: ... To seventeen microliters of the eluates were added 2 μL of 10x Nucleoside Digestion Mix reaction buffer and 1 μL of Nucleoside Digestion Mix (New England Biolabs). Reactions were incubated at 37 °C for 1 hour ...
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bioRxiv - Genomics 2021Quote: ... The PCR mix (8 µl H2O, 2 µl primer mix P5Solexa/P3Solexa, 10 µM each, 20 µl Phusion HF Mix [New England Biolabs]) was added to 10 µl cDNA ...
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bioRxiv - Bioengineering 2021Quote: ... and mixed with two other dsDNA fragments and linearized backbone vector with corresponding overhangs for Golden-Gate-based assembly mix (BsmBI or BsaI golden-gate assembly mix from New England Biolabs). For the pegRNA cloning backbone ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 2 μg of sheared genomic DNA was repaired and end-prepped with NEBNext FFPE DNA Repair Mix and Ultra II End-prep enzyme mix (NEB) for 15 minutes at 20°C followed by 5 minutes at 65°C for inactivation of the enzymes and purified with 1X AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Plant Biology 2023Quote: ... between 0.9 and 1.8 μg of each type of genomic DNA was repaired and end-prepped with NEBNext FFPE DNA Repair Mix and Ultra II End-prep enzyme mix (NEB) for 5 minutes at 20°C followed by 5 minutes at 65°C for inactivation of the enzymes ...
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bioRxiv - Microbiology 2023Quote: The eluted gDNA was resuspended by pipetting to make the beads and elution as homogenous as possible before 20 µL was used in a 40 µL digest using 1 µL of nucleoside digestion mix and 4 µL 10X buffer following the protocol for Nucleoside Digestion Mix (NEB) in an unskirted PCR plate ...
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bioRxiv - Genomics 2022Quote: ... and Ultra II End-prep enzyme mix (NEB) incubated at 20°C for 30 min and at 65°C for 30 min according to ONT protocol ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5 μl of 10 mM dNTP mix (NEB), 2.5 μl of 100 mM DTT (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5 μl of 10 mM dNTP mix (NEB), 2.5 μl of 100 mM DTT (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... 3 μl 10 mM dNTP mix (N0477, NEB), and 3 μl Klenow Fragment (exonuclease-deficient ...
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bioRxiv - Biophysics 2021Quote: ... PCR reaction and a KLD enzyme mix (NEB). The resulting plasmids were transformed into chemically competent DH5α cells and sequences were confirmed by Sanger double stranded DNA sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... using the KLD enzyme mix (New England Biolabs). Brp-GAL4 insertion construct ...
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bioRxiv - Microbiology 2022Quote: ... Gibson Assembly mix (New England Biolabs – MA, USA), and PCR amplification (Qiagen - Hilden ...
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bioRxiv - Immunology 2022Quote: ... dNTP mix (2 mM each) (New England Biolabs), 50U Superscript III RTase ...