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Citations for New England Biolabs :
351 - 400 of 5823 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... PCR was performed for 12 cycles using NEBnext High-Fidelity 2X PCR Master Mix (New England Biolabs, M0541L) (41) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and PCR products were purified with Monarch PCR and DNA Cleanup kit (New England Biolabs, Ipswich, Massachusetts, USA).
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bioRxiv - Zoology 2024Quote: ... PCR products were purified with a Monarch PCR & DNA clean-up kit (New England Biolabs, Whitby, ON, Canada) and used as template to reamplify the products to incorporate a Kozak translation initiation sequence prior to the start codon (Kozak ...
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bioRxiv - Molecular Biology 2023Quote: ... three 25 μl PCR reactions were pooled and purified using Monarch PCR & DNA Cleanup Kit (5 μg; NEB), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and amplified by PCR for 11 cycles using NEBNext High Fidelity 2x PCR Master Mix (New England Biolabs). PCR products were purified using a PCR Purification Kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... PCR amplification of the dhodh locus was performed using Phusion High-Fidelity PCR Master Mix (New England BioLabs) as per the protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... and amplified PCR fragments were purified by column purification using the Monarch PCR Cleanup Kit (New England Biolabs) following the manufacturer’s protocols.
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bioRxiv - Molecular Biology 2024Quote: ... Each PCR reaction (25 µl) contained 12.5 µl NEBNext® High-Fidelity PCR Master Mix (New England Biolabs) with 400 nM of each primer and 50 ng template genomic DNA ...
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bioRxiv - Genomics 2024Quote: ... A 50 µL PCR reaction was prepared containing 1X NEBNext® High-Fidelity PCR Master Mix (NEB #M0541L), 1 µM Ex situ custom forward primer ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products were analyzed by agarose gel electrophoresis and purified with Monarch PCR and DNA Cleanup Kit (NEB). On a passivated glass coverslip ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Transposed DNA was originally amplified and barcoded in a PCR reaction using NEBnext PCR master mix (NEB, M0544) and 1.25 μM forward and reverse primers originally described in 99 ...
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bioRxiv - Microbiology 2024Quote: ... the PCR amplicons were purified used the Monarch PCR and DNA Cleanup Kit (New England Biolabs, Whitby, Canada) and sent for Sanger sequencing at the McMaster Genomics facility ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR was performed upon isolated gDNA using the Phusion High-Fidelity PCR Mastermix (New England BioLabs, Ipswich, MA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and FLAG_Downstream_Fwd and FLAG_Downstream_Rev by PCR with High-Fidelity PCR Master Mix with HF buffer (New England Biolabs #M0531S) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... PCR amplifications were performed with Phusion® High-Fidelity PCR Master Mix with GC Buffer (New England Biolabs) with 500 nM primers ...
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bioRxiv - Cancer Biology 2024Quote: ... The GESTALT cassette was amplified by PCR using Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) with the following conditions ...
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bioRxiv - Developmental Biology 2023Quote: ... along with the guide RNA at a concentration of 300 ng/μl were mixed in molecular grade water with Cas9 buffer (NEB, Cat No. M0386S). Embryos were injected 6 hrs after egg laying for arm and 3hrs for frizzled4 and wnt7 ...
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bioRxiv - Synthetic Biology 2021Quote: ... or Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2021Quote: PCR-amplified amplicons (Hotstart Q5 polymerase, NEB) were sanger sequenced using Genewiz sanger sequence service under standard manufacture instructions.
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bioRxiv - Molecular Biology 2021Quote: ... were subjected to a PCR test (NEB Taq Polymerase ...
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bioRxiv - Developmental Biology 2020Quote: PCR products were digested with BsrI (NEB). Fragment sizes are 188 bp + 221 bp for wild type allele and 409 bp for vegfabmu155 allele.
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bioRxiv - Developmental Biology 2020Quote: PCR products were digested with BccI (NEB). Fragment sizes are 116 bp + 123 bp for wild type allele and 239 bp for vegfaamu128 allele.
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bioRxiv - Physiology 2021Quote: ... and Monarch PCR Cleanup Kit (NEB, T1030). PCR products were electrophoresed in 2% agarose gel (TopVision Agarose Tablets ThermoFisher R2801 ...
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Plant Trans-Golgi Network/Early Endosome pH regulation requires Cation Chloride Cotransporter (CCC1)bioRxiv - Plant Biology 2021Quote: ... following a Phusion® PCR protocol (NEB). The PCR product and the binary plasmid pMDC43 were subsequently cut with the restriction enzymes HindIII-HF and KpnI-HF to remove the 2×35S promoter ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR amplification followed the recommended protocol (NEB) with 12 PCR cycles and a 50 sec ...
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bioRxiv - Biochemistry 2021Quote: ... Colony PCR (OneTaq polymerase, New England Biolabs) with the primer pairs clp2_KO_A–pLSV101_seq rev and pLSV101_seq fwd–clpP2_KO_D (see Tables 2 and 3 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Monarch PCR & DNA Cleanup Kit (NEB) were used in this study for DNA purification.
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bioRxiv - Microbiology 2020Quote: ... Colony PCRs used OneTaq DNA Polymerase (NEB). Restriction enzymes (NEB ...
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bioRxiv - Developmental Biology 2022Quote: ... we used Q5 PCR mix (NEB, M0492L) to amplify the libraries for high-throughput sequencing in Illumina platform.
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bioRxiv - Molecular Biology 2022Quote: ... PCR with taq DNA polymerase (NEB M0273S) followed by agarose gel electrophoresis was used to screen for the presence of the 3xFLAG tag and the YPWM-AAAA mutation ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR-amplified (NEBNext Ultra II Q5; NEB) using primers DCF01 5’-CTTGTGGAAAGGACGAAACACCG-3’ and DCR03 5’-CCTAGGAACAGCGGTTTAAAAAAGC-3’ and subjected to single-end 75 bp (SE75 ...
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CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen productionbioRxiv - Plant Biology 2021Quote: ... the Monarch PCR & DNA Cleanup Kit (NEB), or SPRI magnetic nucleic acid purification beads (UC Berkeley DNA Sequencing Facility) ...
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bioRxiv - Microbiology 2022Quote: ... using colony OneTaq PCR (New England Biolabs). For protease activity ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were treated with DpnI (NEB) and purified using a gel extraction kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... PCR amplicons were digested using PvuII (NEB) restriction enzyme at 37°C for 1 hour and were run on an agarose gel ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were amplified with Phusion (NEB), following the manufacturer’s specifications ...
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bioRxiv - Synthetic Biology 2021Quote: ... Monarch PCR & DNA Cleanup Kit (NEB #T1030L) was used to purify the fragments and they were all adjusted to the concentration of 100 nM to use for active learning experiments.
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bioRxiv - Biochemistry 2021Quote: ... Colony PCR (OneTaq polymerase, New England Biolabs) with the respective primer pairs clp_A–pLSV101_seq rev and pLSV101_seq fwd–clp_D (Table 2 ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR amplicons were column purified (NEB, T1030) and used as a template for in vitro transcription reactions with a mMESSAGE mMACHINE® T7 Transcription Kit (Ambion ...
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bioRxiv - Microbiology 2021Quote: ... PCR fragments were gel purified (NEB T1020L) and cloned into pMiniT 2.0 vector (NEB E1202S) ...
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bioRxiv - Genomics 2020Quote: ... PCR was performed using Phusion HF (NEB) and TruPE PCR primers (Illumina) ...
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bioRxiv - Genetics 2020Quote: ... PCR was performed with Q5 polymerase (NEB) following manufacturer’s instructions with 10 PCR cycles (primers listed in Table S1) ...
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bioRxiv - Microbiology 2021Quote: ... PCR with Q5 High Fidelity Polymerase (NEB) was used to generate insert and backbone DNA ...
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bioRxiv - Genetics 2023Quote: ... All libraries were PCR amplified (NEB M0544S) for 5 cycles ...
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bioRxiv - Cell Biology 2023Quote: ... PCR was performed using Phusion polymerase (NEB). Unique Fluidigm sample barcodes were added using Fast Start High Fidelity PCR System (Roche) ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR products and NdeI/XhoI (NEB, USA) digested pAN001 vector DNA were assembled using Gibson assembly standard protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Q5 PCR Master Mix (New England BioLabs) was used for PCR reactions ...
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bioRxiv - Molecular Biology 2022Quote: ... mTurbo was amplified by pfusion PCR (NEB) using Ti833/Ti834 as primers and 3xHA-miniTurboNLS_pCDNA3 (addgene #107172 ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were digested by ExoI (NEB) to remove excess of oligos and purified using Qiagen PCR purification kit ...
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bioRxiv - Genomics 2024Quote: ... 20uL 2X NEBnext PCR master mix (NEB) was added ...