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Citations for New England Biolabs :
251 - 300 of 5823 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... PCR using Standard Taq Polymerase (NEB) was performed for 30 cycles of (denaturation ...
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bioRxiv - Synthetic Biology 2021Quote: Phusion PCR reactions (New England Biolabs) were used to generate backbone fragments for library assembly ...
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bioRxiv - Synthetic Biology 2021Quote: Phusion PCR reactions (New England Biolabs) were used to generate amplicons for Illumina NGS from purified plasmid DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... by PCR with Q5 polymerase (NEB) using primers petSUMO_F ...
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bioRxiv - Molecular Biology 2021Quote: ... NEB Phusion PCRs (NEB; Cat #: M0531S) were conducted from reverse transcriptase reactions generated from the SuperScript™ VILO™ cDNA Synthesis Kit (Thermofisher ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR reactions used Phusion polymerase (NEB) or PrimeStarGXL polymerase (Takara ...
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bioRxiv - Neuroscience 2020Quote: ... Luna qRT-PCR Master Mix (NEB) was used with the primers ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR was performed with OneTaq (NEB), following the manufacturer’s specifications ...
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bioRxiv - Evolutionary Biology 2021Quote: ... High-fidelity PCR Master Mix (BioLabs) and a touch-down annealing process were used for amplification ...
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bioRxiv - Microbiology 2020Quote: ... and OneTaq PCR Master Mix (NEB). Reverse primers for most sgRNAs also contained a unique KpnI or BglII site to assist clone identification by restriction fragment analysis ...
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bioRxiv - Microbiology 2021Quote: ... After PCR with Q5 polymerase (NEB), the product was treated with DpnI (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... Phusion PCR reactions (New England Biolabs) were used to amplify these extended oligos ...
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bioRxiv - Systems Biology 2023Quote: ... and PCR master mix (NEB, M0543S). The amplification began with a 30-second denature step at 98°C ...
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bioRxiv - Cell Biology 2023Quote: ... Following PCR using Q5 polymerase (NEB), the product is gel-purified on a 1% agarose gel ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted by NEB PCR Cleanup Kit (NEB Cat. # T1030), followed by quality control steps using Qubit fluorometer and bioanalyzer ...
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bioRxiv - Systems Biology 2022Quote: ... and PCR Master Mix (NEB, M0543S) for each reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... OneTaq RT-PCR mix (NEB #E5315) was used to amplify the generated cDNA.
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bioRxiv - Molecular Biology 2024Quote: ... PCR amplification using Phusion polymerase (NEB) with selective primes (fwd ...
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bioRxiv - Systems Biology 2022Quote: ... and PCR master mix (NEB, M0543S). We used 12 cycles to amplify the cDNA using the following protocol ...
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bioRxiv - Cell Biology 2023Quote: All fragments were PCR purified (NEB), ligated using NEB HiFi assembly kit ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by PCR with Q5 (NEB) and gel electrophoresis to size-select the final library ...
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bioRxiv - Plant Biology 2024Quote: ... amplified by Phusion PCR (NEB, UK).
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bioRxiv - Biochemistry 2024Quote: ... PCR amplified (Q5 DNA polymerase; NEB) and purified (PureLink PCR column ...
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bioRxiv - Systems Biology 2020Quote: ... 0.4 μL 10 mM dNTP Solution (New England Biolabs), 4 μL 5x Phusion HF Buffer (Thermo Scientific) ...
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bioRxiv - Systems Biology 2023Quote: ... 0.4 μL 10 mM dNTP solution (New England Biolabs), 4 μL 5× phusion HF buffer (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... and deoxynucleotide solution (both from New England Biolabs, NEB). The resulting PCR product was integrated into the plasmid via the Gibson Assembly cloning system (New England Biolabs-NEB) ...
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bioRxiv - Microbiology 2022Quote: ... and deoxynucleotide solution (both from New England Biolabs, NEB). The resulting PCR product was integrated into the plasmid via the Gibson Assembly cloning system (New England Biolabs-NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... 2 uL dNTP Solution Mix (10 mM stock, NEB), 2 μl Taq DNA ligase (80 U ...
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bioRxiv - Immunology 2023Quote: ... and 25ul 10X DNaseI enzyme reaction solution (NEB, M0303L) were added and incubated at 37°C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... 0.125 µl of molecular grade bovine serum albumin (20,000 ng/µl stock, catalog no. B9000S, New England Biolabs, Inc., Ipswich, MA, USA), 1 µl of DNA ...
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bioRxiv - Genetics 2021Quote: ... PCR amplification was performed in a 10 µl reaction volume containing 1X PCR Buffer (New England Biolabs), 10 µM dNTP and 0.05 U Taq DNA polymerase (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We then performed PCR reactions at 12 cycles using Phusion High-Fidelity PCR Master Mix (NEB Biolabs) and primers that bind to common regions in the adaptors ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We then performed PCR reactions at 12 cycles using Phusion High-Fidelity PCR Master Mix (NEB Biolabs) and primers that bind to common regions in the adaptors ...
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... PCR was performed according to manufacturer’s protocol (Phusion High-Fidelity PCR kit, New England Biolabs, Ipswich, MA).
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bioRxiv - Genetics 2021Quote: ... The A-tailed PCR products were cleaned with Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB) and ligated into pGEM®-T Easy vector (Promega ...
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bioRxiv - Microbiology 2021Quote: ... All PCR steps were carried out using the Phusion High-Fidelity PCR Master Mix (New England Biolabs). The PCR products were electrophoresed on a 2% agarose gel and stained with SYBR Safe DNA gel stain ...
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bioRxiv - Systems Biology 2022Quote: ... PCR amplification was performed on the eluate with an NEBNext 2X High Fidelity PCR Mix (NEB, M0541S) N7 ...
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bioRxiv - Genetics 2022Quote: ... Phusion® High-Fidelity PCR Master Mix was used to prepare the PCR library (New England Biolabs). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... Random mutagenesis was performed using Error-Prone PCR (EP-PCR) with Taq DNA polymerase (New England Biolabs). PCR products and restriction digestion products were purified using a GeneJET gel extraction kit (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix (New England Biolabs). Mix same volume of 1×loading buffer (contained SYBR green ...
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bioRxiv - Genetics 2020Quote: ... DNA libraries were then prepared by PCR amplification using NEBNext High-Fidelity PCR kit (New England Biolabs) in the presence of barcoded PCR primers (sequences provided in Ref ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix (New England Biolabs), and the mixture PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... the scFv containing region from isolated plasmids was amplified by PCR using Q5 high fidelity PCR (NEB). Illumina NGS samples were prepped and run using the Illumina MiSeq v3 reagent kit following manufacturer’s protocols pooling four library samples per lane ...
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bioRxiv - Genetics 2021Quote: ... PCR amplification was done with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Genomics 2021Quote: ... first-level PCR reactions were performed using 1μl PCR-compatible lysate as a template for a 6.25μl Q5 (NEB) PCR reaction according to the manufacturer’s protocol (annealing temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitive PCR and limited-cycle PCR was performed using NEBNext Ultra II master mix (New England Biolabs), SYBR™ green and 0.5 µM of sample-specific P5/P7 barcoded primer mix (New England Biolabs) ...
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bioRxiv - Genomics 2023Quote: ... The PCR products were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, no. T1030L). In vitro transcription of pegRNAs and gRNAs was performed using the HiScribe SP6 RNA Synthesis Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplification was performed with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... The PCR fragments of interest were treated with the same enzymes and cleaned by PCR cleanup (NEB). Linear plasmid pJAM2678 and each PCR product were ligated together in a reaction using T4 DNA ligase overnight at 16 °C to obtain plasmid pAKS202 that contained 150 bp upstream of the translation start site of hvo_B0047 cloned in front of bgaH ...
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bioRxiv - Cell Biology 2023Quote: ... Synthesized testis cDNAs were used for endpoint PCR or quantitative PCR using OneTaq 2X Master Mix (NEB) or with iTaq Universal SYBR Green Supermix (BIO-RAD) ...