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Citations for New England Biolabs :
201 - 250 of 5823 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... PCR purifications with the Monarch® PCR & DNA cleanup kit (New England Biolabs, Ipswich, USA), extraction of genomic DNA with the Monarch® Genomic DNA Purification Kit (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA was then PCR-amplified using barcoded PCR primers (New England Biolabs #E7335L, #E7500L). After AMPure XP bead purification (Beckman Coulter #A63881 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplicons were purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs).
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bioRxiv - Cell Biology 2024Quote: ... The PCR product was purified using the Monarch PCR and DNA cleanup kit (T1030S, NEB) and dsRNA was synthesised using the T7 RiboMAX express system (P1700 ...
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bioRxiv - Bioengineering 2024Quote: ... PCR reactions were purified using the Monarch DNA PCR Cleanup Kit (New England Biolabs; T1030). For Gibson assembly of constructs ...
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bioRxiv - Genomics 2024Quote: ... The pooled DNA was PCR-amplified using Phusion® High-Fidelity PCR Kit (NEB®), followed by purification with a Monarch® PCR & DNA Cleanup Kit (NEB®) ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was cloned using NEB® PCR Cloning Kit (New England Biolabs, #E1202S) where 80ng of the purified PCR product was ligated into a linearized pMiniT 2.0 vector and transformed into the NEB® stable competent 10-beta E ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were then PCR purified using a Monarch® PCR & DNA cleanup kit (NEB-T1030S) once a band of the correct size was visualized on a 1% Agarose gel ...
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bioRxiv - Synthetic Biology 2024Quote: ... Genomic DNA samples were PCR amplified with Q5 High-Fidelity 2X PCR Master Mix (NEB) based on the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... PCR reactions were purified with a Monarch PCR & DNA Cleanup Kit (New England Biolabs, T1030) and spot checked for purity via gel electrophoresis ...
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bioRxiv - Plant Biology 2024Quote: ... PCR products were purified using the Monarch PCR and DNA Cleanup kit (New England Biolabs) and then Sanger sequenced (Genewiz ...
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR amplifications are then column-purified using the Monarch PCR & DNA Cleanup Kit (NEB) and assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Genetics 2024Quote: Plasmid cloning was performed using PCR amplicons generated with Q5 PCR polymerase (New England Biolabs). DNA fragments were assembled by either In-Fusion cloning (Takara ...
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bioRxiv - Synthetic Biology 2024Quote: ... The area of the coverslips around the tissue slices was then dabbed dry with a Kimwipe before mounting a SecureSeal hybridization chamber (Grade Biolabs #621502) on top of the tissue slices ...
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bioRxiv - Biophysics 2023Quote: ... Trypsin digestion was initiated by adding 1:10 (trypsin to protein, m/m) mass spectrometry grade trypsin (New England Biolabs #P8101S) in 50 mM ammonium bicarbonate ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products from all reactions were purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Desired PCR cycle numbers were determined in test endpoint PCRs using Q5 DNA polymerase (NEB M0491L). Final HiC libraries were generated from 4-6 individual PCR reactions ...
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bioRxiv - Genetics 2021Quote: ... and libraries were generated by PCR with NEBNext High Fidelity 2X PCR Master Mix (NEB M0541). Library size was confirmed on a BioAnalyzer prior to sequencing on the NextSeq 550 (40bp read length ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR reaction was column-purified using the Monarch PCR & DNA Cleanup Kit (New England BioLabs). The IVT reaction including DNAseI digest was performed with the mMESSAGE mMACHINE T7 Transcription Kit (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: PCR products were extracted with Monarch PCR & DNA Gel Cleanup Kit (New England Biolabs, Ipswich, MA) and sequenced by ABI 3730 automated DNA sequencer.
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bioRxiv - Molecular Biology 2022Quote: ... Site-directed mutagenesis was performed by PCR with NEBNext High-Fidelity 2x PCR master mix (NEB) using overlapping primers with 15 nt overhangs carrying the desired mutation (IDT).
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bioRxiv - Neuroscience 2021Quote: ... Kv1.2wt-T2A and Kir2.1wt-T2A fragments were magnified by PCR (Phusion High-Fidelity PCR Kit, NEB) from pcDNA3-Kv1.2wt (human cDNA template ...
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bioRxiv - Genetics 2022Quote: ... Each PCR consisted of: 20 µL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 2 µL 10 µM i5 indexing adapter ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription and subsequent PCR amplification was done with OneTaq RT-PCR Kit (New England Biolabs). Primers ...
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bioRxiv - Immunology 2020Quote: ... PCR was performed with a Phusion High-Fidelity PCR Kit (New England Biolabs, Ipswich, MA, USA) in 20µL reactions mixed with 2µL cDNA and 10µM primers ...
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bioRxiv - Microbiology 2020Quote: ... and PCR mixes using the Q5® High-Fidelity PCR Kit (New England Biolabs, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... All PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix (New England Biolabs). The PCR products were mixed with an equal volume of 1× loading buffer (containing SYB green ...
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bioRxiv - Genetics 2021Quote: ... PCR products were purified using NEBMonarch PCR and DNA Clean-up Kit (New England Biolabs, USA), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were purified using the Monarch® PCR & DNA clean-up kit (New England Biolabs). In vitro expression was performed for 2 h at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... The resulting PCR products were purified using the Monarch® PCR & DNA Cleanup Kit (NEB, T1030) and used as “megaprimers” that are denatured and annealed to the original plasmid (pNG93 ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR reaction was performed using NEBNext High Fidelity PCR Master Mix (NEB, catalog no. M0541L) for 20 cycles ...
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bioRxiv - Molecular Biology 2022Quote: ... Post-capture PCR was performed with the NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541S) for 14-20 cycles ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was reverse-transcribed and PCR amplified using Luna Universal One-Step RT-PCR Kit (NEB). SARS-CoV-2 replication was assessed by using primers specific to the N mRNA (Forward 5’-CTCTTGTAGATCTGTTCTCTAAACGAAC-3’ ...
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bioRxiv - Synthetic Biology 2024Quote: All PCRs were carried out using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB), gel extractions using the QIAquick Gel Extraction Kit (QIAGEN ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCRs were carried out using the Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB) in a total volume of 20ul ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were carried out using the Phusion High-Fidelity PCR Master Mix (New England Biolabs). The PCR products were selected by 2% agarose gel electrophoresis ...
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bioRxiv - Genomics 2022Quote: ... The PCR product was purified using the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L). For the last PCR ...
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bioRxiv - Biochemistry 2023Quote: PCR mutagenesis was performed by whole plasmid PCR with Q5 high-fidelity polymerase (New England Biolabs) followed by PCR purification (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... Each PCR consisted of: 20 μL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 1 μL 10 μM forward primer ...
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bioRxiv - Biochemistry 2023Quote: ... RT-PCR reactions were performed using the OneTaq One-Step RT-PCR Kit (New England BioLabs) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... General PCR for cloning was performed using the NEBNext PCR master mix (NEB cat no. M0541L), with 500 nM of forward and reverse primers ...
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bioRxiv - Evolutionary Biology 2024Quote: ... After the reaction we purified the PCR product with Monarch PCR and DNA Cleanup Kit (NEB).
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bioRxiv - Cell Biology 2024Quote: ... Eluted DNA was enriched by PCR using Phusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Biophysics 2024Quote: ... PCR was followed by the addition of the PCR product to a KLD enzyme mix (NEB) for template removal ...
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bioRxiv - Genetics 2021Quote: ... using Q5 polymerase PCR products (NEB). pCMV_AncBE4max (Addgene plasmid #112094 ...
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bioRxiv - Molecular Biology 2021Quote: ... USA (NEB PCR Cloning Kit E1203S) were used for the sub-cloning of DNA fragments and to create plasmid templates for run-off in vitro transcription ...
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bioRxiv - Genomics 2020Quote: ... 50μl PCR reactions using Q5 (NEB) were cycled 98°C 1min ...
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bioRxiv - Genetics 2021Quote: ... PCR reagents were purchased from NEB, and all primers were synthesized by Integrated DNA Technologies (IDT).
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bioRxiv - Microbiology 2021Quote: ... PCR amplification by Taq polymerase (NEB) using primers for internal regions of KL ...
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bioRxiv - Neuroscience 2021Quote: ... Luna qRT-PCR Master Mix (NEB) was used with the primers ...