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Citations for New England Biolabs :
3801 - 3850 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Fragments were purified using the Monarch PCR & DNA Cleanup Kit (NEB T1030) after digestion or amplification ...
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bioRxiv - Microbiology 2020Quote: ... PCRs were performed with the Phusion High-Fidelity DNA polymerase from NEB, using the Phusion GC Buffer ...
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bioRxiv - Microbiology 2020Quote: ... 5 μL 2x NEBnext High-Fidelity PCR Master Mix (New England Biolabs) and 2.9 μL nuclease-free distilled H2O (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... which were added via PCR using Phusion DNA polymerase (New England BioLabs). After PCR ...
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bioRxiv - Microbiology 2021Quote: ... and all PCRs were performed with Q5 High Fidelity DNA Polymerase (NEB). Monomeric enhanced GFP fused to N-terminus of ZAP via a flexible linker (GGGGSGGGGSGGGG ...
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bioRxiv - Molecular Biology 2021Quote: ... SNAPf_6xHis was amplified from this construct by PCR using VENT Polymerase (NEB), forward primer 5’-AAAAGAATTCAACCATGGACAAAGACTGCGAAA-3’ and reverse primer 5’-TTTTGAATTCATTAGTGATGGTGATGGTGATGGGA-3’ (EcoR1 site are underlined) ...
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bioRxiv - Molecular Biology 2020Quote: ... Standard molecular biology techniques including PCR and Gibson assembly (New England Biolabs) were used to make all plasmids and homology repair constructs for all yeast transformations ...
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bioRxiv - Molecular Biology 2021Quote: ... A PCR cocktail consisting of NEBNext Ultra II Q5 master mix (NEB) and Illumina TruSeq PCR primers (RP-1 ...
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bioRxiv - Immunology 2021Quote: ... PCRs were performed with Q5® High-fidelity DNA polymerase (NEB, M0491L) plus GC buffer with the following reaction conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... All PCR reactions were performed using Q5 High Fidelity DNA polymerase (NEB). The basis for all mammalian expression vectors was pc-DNA-puro described previously (9) ...
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bioRxiv - Genetics 2019Quote: ... Flag-Mad-8 was generated by PCR followed by Gibson assembly (NEB) and the S359L substitution was verified by sequencing ...
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bioRxiv - Genomics 2021Quote: ... For Smartseq3xpress with NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB), PCR mastermix consisted of (1x NEBNext Q5 Hot Start HiFi PCR Master Mix ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR was carried out by Q5 HiFi DNA polymerase (New England Biolabs). Primers used for SDM are included in Supplementary datasheet 3 ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR was carried out in 50 μl reactions using Q5 polymerase (NEB) according to manufacturer’s recommendations with 1 ng of the RGR plasmid as template and 500 nM of the following primers (lower case indicating nucleotides added for cloning purposes and restriction site underlined) ...
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bioRxiv - Bioengineering 2022Quote: ... then PCR amplified using the Q5 High-Fidelity 2X Master Mix (NEB). For the tetrahedra with 5 and 7 helical turns per edge ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR was performed with Hot Start Taq Polymerase (M0495S, NEB, Ipswich, MA). PCR condition for MAPK3 ...
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bioRxiv - Bioengineering 2022Quote: ... PCR amplification products were all generated using Phusion High-Fidelity Polymerase (NEB) and all restriction digests were carried out using NEB High-Fidelity restriction enzymes unless stated otherwise.
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bioRxiv - Biochemistry 2022Quote: ... The PCR product was inserted into the linearised (SalI, XbaI from NEB) pRS1024 yeast expression vector (Kellosalo et al. ...
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bioRxiv - Microbiology 2022Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs) or Phusion DNA Polymerase (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were transcribed with T7 HiScribe kit (New England Biolabs) and transfected together with TrueCas9 protein v2 (Thermo Fisher ...
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bioRxiv - Genetics 2022Quote: ... PCR products were subjected to cutting with I-SceI (New England Biolabs) for 1 hour at 37°C then run on an agarose gel ...
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bioRxiv - Genetics 2022Quote: ... rx3 and kcnh6a were PCR amplified with Q5 polymerase (New England Biolabs) and locus-specific primers 5’ extended with partial Illumina adapter sequences (see table below) ...
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bioRxiv - Microbiology 2022Quote: ... Routine PCR analysis was conducted with Taq DNA polymerase (New England Biolabs). PCR for cloning purposes was performed with Q5 DNA polymerase (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... Six PCR reactions using the Q5® High-Fidelity DNA Polymerase (NEB) were performed to replace WU-Fragment 9 and 10 to create the SARS-CoV-2S-Delta ...
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bioRxiv - Microbiology 2022Quote: ... Six PCR reactions using the Q5® High-Fidelity DNA Polymerase (NEB) were performed.
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bioRxiv - Synthetic Biology 2022Quote: PCR was performed using Q5® High-Fidelity DNA Polymerase (M0491, NEB) with DNA oligos from Integrated DNA Technologies (IDT) ...
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bioRxiv - Microbiology 2022Quote: ... Spin-purified PCR products were digested with BsaI-HF-v2 (R3733; NEB) and the size and integrity of full length and digested PCR products were confirmed on a 4% agarose e-gel (Thermo) ...
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bioRxiv - Microbiology 2022Quote: ... qRT-PCR was performed using Luna Universal qPCR Master Mix (NEB; M3003) with SYBR Green ...
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bioRxiv - Molecular Biology 2023Quote: ... The 500bp PCR product was digested with NspI (New England Biolabs, R0602L) that recognizes a RCATG/Y cut site only present in mutants ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was amplified using standard PCR using LongAmp Taq DNA polymerase (NEB) targeting the CRISPR locus with a 200-500 bp long amplicon ...
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bioRxiv - Molecular Biology 2023Quote: ... Barcodes were amplified with 22 cycles of PCR using Phusion polymerase (NEB) with primers that add Illumina adaptor sequences and a 6 bp identifier sequence to distinguish the various drug conditions and time points ...
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bioRxiv - Molecular Biology 2023Quote: PCR of genomic DNA was performed using LongAmp Taq DNA polymerase (NEB) and primers designed against an amplicon of 200-500 bp targeting the CRISPR-locus and the top four CRISPOR-predicted off-targets ...
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bioRxiv - Plant Biology 2023Quote: ... The amplified PCR products were then ligated with T4 DNA Ligase (NEB) and the CRY2 sgRNA genes were subcloned into the modified DSB/DSB PcUbi4-2 plasmids (i.e. ...
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bioRxiv - Developmental Biology 2024Quote: ... Eluted DNA was amplified using NEBNext Ultra II PCR Master Mix (NEB) and purified using AMPure XP beads ...
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bioRxiv - Neuroscience 2022Quote: ... The PCR product was cut with BamH1 and Xho1 (New England Biolabs) and cloned directly into pCS2R cut with the same enzymes ...
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bioRxiv - Physiology 2022Quote: ... PCR was performed with Phusion High-Fidelity DNA polymerase (New England Biolabs), Universal PCR primers and Index (X ...
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bioRxiv - Molecular Biology 2022Quote: ... The post-PCR microcapsules were treated with 100 U Exonuclease I (NEB) for 15 minutes at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA was PCR amplified with Phusion high-fidelity DNA Polymerase (NEB) using barcoded Illumina PCR index primers ...
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bioRxiv - Genomics 2022Quote: ... Eluted DNA was amplified using NEBNext Ultra II PCR Master Mix (NEB) and purified using AMPure XP beads ...
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bioRxiv - Microbiology 2024Quote: Polynucleotides were synthesized by PCR using Phusion High-Fidelity DNA polymerase (NEB) and locus-specific primers (see Table S2 for sequences) ...
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bioRxiv - Microbiology 2024Quote: All PCR reactions were performed using Phusion HF polymerase (New England Biolabs). All oligonucleotides were purchased from Integrated DNA Technologies (IDT) ...
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bioRxiv - Developmental Biology 2024Quote: ... IVT sgRNA products were purified Monarch PCR and DNA Cleanup Kit (NEB) and quantified ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products were assembled using NEB Hifi DNA Assembly Master Mix (NEB) according to the manufacturer instructions ...
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bioRxiv - Genetics 2024Quote: ... We ligated the PCR fragments into the vectors using T4 ligase (NEB), transformed into E ...
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bioRxiv - Biochemistry 2024Quote: ... Libraries were PCR amplified using Phusion DNA Polymerase (M0530, New England Biolabs). Quality assessment of the libraries and sequencing were performed by the NGS platform of the University of Bern.
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bioRxiv - Cancer Biology 2024Quote: ... and a PCR based insert produced by Phusion DNA Polymerase (NEB, #M0530L) containing a second sgRNA cloning site (SbfI) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR was performed using the Q5 High-Fidelity DNA Polymerase Kit (NEB) and primers specific for the microsatellite expansion adapters (1X Q5 Reaction Buffer ...
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bioRxiv - Microbiology 2024Quote: The rsmA gene was PCR amplified using Phusion polymerase (New England BioLabs) according to the manufacturer specifications ...
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bioRxiv - Molecular Biology 2024Quote: ... Library was prepared with NEBNext High-Fidelity 2X PCR Master Mix (NEB) and SYBR Green I (ThermoFisher) ...
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bioRxiv - Plant Biology 2024Quote: ... PCR amplification was done using Phusion® High Fidelity DNA Polymerase (NEB) with an annealing temperature of 60°C and an extension time of 30 seconds ...