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Citations for New England Biolabs :
3651 - 3700 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Barcoding and enrichment of libraries was carried out using NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs; New England Biolabs, Cat. No. E6440). Paired-end sequencing (2×151bp reads ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... mRNA Magnetic Isolation Module in combination with the NEBNext® Ultra™ II Directional RNA Library Prep Kit and indexed with NEBNext® Multiplex Oligos for Illumina® (96 Index Primers) (New England Biolabs, Ipswich, Massachusetts, USA). Fragmentation was carried out using incubation conditions recommended by the manufacturer for samples with a RIN ≥7 to produce RNA sizes ≥ 300bp (94°C for 10 minutes ...
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bioRxiv - Genomics 2023Quote: ... BAC baits were PCR amplified using 5’ biotinylated forward and reverse primers (Supplementary Table S7) in the following reaction mix: 10 µl 2X NEBNext® Ultra™ II Q5® Master Mix (NEB cat. M0544S), 1 µl 10 µM each bio-primer ...
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bioRxiv - Genomics 2024Quote: All adapter-ligated amplicons were amplified with custom iTru5 and iTru7 index primers (Glenn et al. 2019) with NEBNext Ultra II Q5 Master Mix (NEB, Cat No, M0544, Ipswich, MA) according to reaction conditions described in Supplemental Table 6 ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was mixed with Reaction Mix 1 (9:1 of 12 uM NEB RT random 6N-S to 12 uM oligo-dT(20), 12uM dNTPs ...
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bioRxiv - Microbiology 2023Quote: ... and the expression levels of genes were quantified with the Luna® universal one-step RT-qPCR kit (New England Biolabs, Ipswich, MA, USA) with the following cycling parameters ...
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bioRxiv - Microbiology 2024Quote: ... RNA concentration and quality were evaluated using NanoDrop 2000 Spectrophotometer (Thermo Fischer Scientific) and reverse-transcribed to cDNA using LunaScript® RT SuperMix Kit (New England Biolabs, Cat no. E3010) with random hexamers and oligo dT ...
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bioRxiv - Plant Biology 2020Quote: ... ChIP DNA was purified with the Monarch PCR & DNA Cleanup Kit (NEB). Libraries were prepared using ThruPLEX DNA-seq Kit (Rubicon Genomics ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR was conducted using Q5 HotStart High Fidelity Polymerase (NEB, Ipswich, MA) using forward primer ...
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bioRxiv - Genetics 2019Quote: ... All PCR-amplifications were conducted using Phusion High-Fidelity DNA Polymerase (NEB). C ...
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bioRxiv - Microbiology 2020Quote: ... 25 μL 2x NEBnext High-Fidelity PCR Master Mix (New England Biolabs) and 10 μL nuclease-free distilled H2O (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... Oligos were amplified twice by PCR (Phusion High-Fidelity DNA Polymerase, NEB) following manufactural instruction ...
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bioRxiv - Developmental Biology 2021Quote: ... Obtained PCR products with digested vector were assembled by Gibson assembly (NEB) following manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2021Quote: ... Ten PCR cycles with Phusion High-Fidelity DNA polymerase (New England Biolabs) were used for library amplification with indexed adaptors ...
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bioRxiv - Cell Biology 2019Quote: ... then purified with Monarch PCR & DNA Purification Kit (New England BioLabs, T1030S) followed by T4 DNA Ligase reaction (New England BioLabs ...
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bioRxiv - Cell Biology 2019Quote: ... where the PCR product was treated with DpnI (New England BioLabs, R0176S) then purified with Monarch PCR & DNA Purification Kit (New England BioLabs ...
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bioRxiv - Cell Biology 2020Quote: ... by fusion PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs) and used as a homologous repair template ...
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bioRxiv - Genetics 2021Quote: ... All PCRs were performed using the Q5 High-Fidelity DNA Polymerase (NEB). The amplicons were purified using a Universal DNA Purification Kit ...
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bioRxiv - Genetics 2021Quote: ... The resulting PCR products were restriction-digested with EagI (New England Biolabs) for pDEW201-P1iraD and ApaI (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: ... The resulting PCR products were restriction digested with EagI (New England Biolabs) for pDEW201-P1iraD and ApaI (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: Bacterial colony PCR with high fidelity DNA polymerase Phusion (New England Biolabs) was used to obtain the wild type alleles of holC and yoaA for construction of GAL4 fusions for the yeast two hybrid analysis as previously described (16) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Barcodes were amplified by 19 cycles of PCR using Phusion polymerase (NEB) and primers that add Illumina adapter sequences and an 8 bp identifier sequence used to distinguish libraries and time points ...
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bioRxiv - Microbiology 2021Quote: ... The resulting cDNA was PCR amplified with Q5 Hight Fidelity Polymerase (NEB) using LInker-2-partial (5′-TTAGGTGTTGGGTTGGGCCA-3′ ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were digested with restriction enzymes AscI (New England Biolabs) and EcoRV (New England Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... sgRNA was generated by PCR amplification using the Phusion Polymerase (NEB, M0530S) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Digested DNA was purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and mixed with the PCR amplicon in Gibson Assembly Master Mix (NEB; protocol as described by the manufacturer) ...
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bioRxiv - Developmental Biology 2020Quote: ... The purified PCR fragments were digested by SalI/FseI restriction enzymes (NEB) and LV-H2B-GFP backbone was also dephosphorylated (CIP ...
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bioRxiv - Developmental Biology 2020Quote: ... and to screen clones PCR products were digested using HF-HindIII (NEB). Enzyme digests were separated by electrophoresis and genotypes distinguished by fragment banding patterns (wild type ...
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bioRxiv - Microbiology 2022Quote: ... was digested with EcoRV and PCR products inserted by Gibson Assembly (NEB) as previously described ...
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bioRxiv - Neuroscience 2021Quote: ... and Phusion High Fidelity PCR MasterMix (New England Biolabs, Ipswich, MA, USA) and amplified by 1 cycle of 72 °C for 5 min and 98 °C for 30 sec ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR was performed using Q5® High-Fidelity DNA Polymerases (NEB #M0491) and primers F01/R01 (10 ng oligos ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was PCR amplified for 14 cycles by using LongAmp Taq (NEB). ONT adaptor ligation was performed using T4 DNA ligase (NEB) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Candidate cis-regulatory regions were amplified by PCR with OneTaq polymerase (NEB) and cloned into the pCR8 plasmid (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed with Taq 2X Master Mix (New England BioLabs, Maine), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Purified gDNA fragments were then PCR amplified (NEBNext Ultra II Q5, NEB) with FLI1F and FLIR primers in PCR1 (denaturation at 98°C for 30 seconds ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR genotyping was performed using Q5 High-Fidelity 2X Master Mix (NEB) with the primer pairs listed in Table 2 ...
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bioRxiv - Synthetic Biology 2019Quote: ... DNA was amplified using Phusion High-Fidelity PCR Master Mix (NEB, M0531S) and the following conditions ...
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bioRxiv - Genetics 2019Quote: ... Each oligonucleotide was amplified by PCR (Q5 High-Fidelity DNA Polymerase, NEB) for 15 cycles ...
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bioRxiv - Microbiology 2019Quote: ... the two PCR products were purified and then digested with AarI (NEB) enzyme at 37 C for 5 hrs ...
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bioRxiv - Microbiology 2019Quote: All PCR products were synthesized using Phusion HF polymerase (New England Biolabs). Sequencing of constructs and strains was performed at Eurofins Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... The PCR product was ligated using the NEBuilder HiFi DNA assembly (NEB).
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bioRxiv - Microbiology 2019Quote: ... or the Monarch PCR and DNA Cleanup Kit (New England Biolabs, US). After Sanger-sequencing (Eurofins ...
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bioRxiv - Developmental Biology 2019Quote: ... and PCR-amplified using Phusion high-fidelity DNA polymerase (New England Biolabs) together with the following set of primers ...
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bioRxiv - Bioengineering 2019Quote: ... PCR was done using Taq DNA polymerase (New England Biolabs, Cat. # M0267L) with the following recipe ...
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bioRxiv - Neuroscience 2019Quote: ... PCR products were treated with the BstNI restriction enzyme (New England Biolabs) or a control enzyme-free buffer solution and incubated overnight at 60°C to ensure complete digestion ...
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bioRxiv - Genetics 2020Quote: ... The PCR included Q5® High-Fidelity DNA Polymerase (New England Biolabs) to minimize incorporation errors ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCRs were performed with Q5® High-Fidelity DNA polymerase (NEB, M0491L) in parallel reaction volumes of 50 ml with the following cycle conditions ...
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bioRxiv - Developmental Biology 2019Quote: ... The PCR products were digested with T7 Endonuclease I (T7E1, NEB M0302S) according to the recommended protocol36 ...
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bioRxiv - Systems Biology 2019Quote: ... PCRs were conducted using Phusion® High-Fidelity DNA Polymerase (NEB, UK) with a 65 °C primer annealing temperature ...