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Citations for New England Biolabs :
3751 - 3800 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... PCR reactions were carried out with the Phusion DNA polymerase (NEB, M0530S) in the presence of 3% dimethyl sulfoxide and 1 M betaine (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... The PCR product was digested with PspGI restriction enzyme (NEB, catalog #R0611S) to obtain a 1080 bp long DNA with 5 bp overhang and was subsequently dephosphorylated with Antarctic phosphatase (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... PCR was performed with Q5 High-Fidelity DNA Polymerase (New England Biolabs) and the primers listed in Supplementary table 3 ...
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bioRxiv - Genetics 2022Quote: ... 25 µL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 20 µL nuclease-free water ...
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bioRxiv - Genomics 2022Quote: ... Eluted DNA was amplified using NEBNext Ultra II PCR Master Mix (NEB) and purified using AMPure XP beads ...
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bioRxiv - Molecular Biology 2020Quote: ... Genotyping was performed by PCR looking for acquired resistance to NcoI (NEB) cutting and selected clones were confirmed by immunoblotting for STING ...
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bioRxiv - Genetics 2022Quote: ... Amplified and gel-purified PCR ORFs were XbaI (R0145S, NEB, Ipswich, Massachusetts) and PacI (R0547S ...
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bioRxiv - Evolutionary Biology 2022Quote: ... or cloned using the NEB® PCR cloning kit (New Englands BioLabs) to obtain haplotype resolution ...
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bioRxiv - Microbiology 2022Quote: ... BarSeq PCRs were performed as 50 μL reactions with Q5 polymerase (NEB) and 200 ng of template DNA ...
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bioRxiv - Microbiology 2022Quote: ... or PCR amplified from human cDNA using Vent Polymerase (New England Biolabs), then introduced into the luciferase with an intron construct at the EcoRI site ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was carried out using Q5 High-Fidelity DNA Polymerase (NEB #M0491S) with primers (Supplementary Table 1 ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified using the Monarch DNA Gel Extraction Kit (NEB) and eluted in 20 µl ddH2O ...
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bioRxiv - Developmental Biology 2019Quote: ... The sequence was PCR amplified with Phusion High-Fidelity DNA Polymerase (NEB) and primers containing BamHI and ClaI sites and was cloned in-frame with 6 Myc Tag epitopes into the BamHI and ClaI sites of a pCS2-MT plamid ...
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bioRxiv - Bioengineering 2019Quote: PCR amplification of targeted sequences was performed using the Phusion polymerase (NEB) and the OneTaq polymerase (NEB) ...
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bioRxiv - Genetics 2020Quote: ... Amplicons were purified using the Monarch® PCR & DNA Cleanup Kit (NEB) following the manufacturer’s instructions and sent for 250 bp paired end Illumina amplicon sequencing (Genewiz) ...
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bioRxiv - Genetics 2020Quote: ... Libraries were prepared by PCR using Q5 High Fidelity DNA polymerase (NEB) polymerase and using primers carrying Illumina Nextera i7 barcodes ...
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bioRxiv - Synthetic Biology 2019Quote: ... The pCD backbone was assembled by PCR amplifying (Phusion, New England Biolabs) regions of pSR58.6 (B0015 and T0 terminators ...
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bioRxiv - Cancer Biology 2019Quote: ... The resulting PCR fragments were then digested by PstI (New England Biolabs), resolved on 3% agarose gels ...
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bioRxiv - Developmental Biology 2019Quote: ... The supernatant was used directly in genotyping PCR performed with LongAmp (NEB) or PrimeStar GXL (Clontech ...
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bioRxiv - Genetics 2020Quote: ... 25 μL of High-Fidelity 2X PCR Master Mix (New England BioLabs), 2.5 uL of indexing primer and 10 μL of DDW were added to the 10 μL of purified DNA and the DNA was amplified five cycles using the following PCR program ...
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bioRxiv - Developmental Biology 2020Quote: ... and amplification was performed in 1 × next PCR master mix (NEB, M0541S) and 1.25 μM of custom Nextera PCR primers 1 and 2 with the following PCR conditions ...
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bioRxiv - Immunology 2019Quote: ... PCR products and vector were ligated using high fidelity assembly mix (NEB) into expression plasmids adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK2 vectors (Invivogen) ...
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bioRxiv - Systems Biology 2020Quote: ... Colony PCRs were performed using Taq polymerase (New England Biolabs, Ipswich, England) with primers P192 ...
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bioRxiv - Synthetic Biology 2020Quote: ... linear transposon cassettes were constructed by standard Q5 Hotstart PCR protocol (NEB) with TN5MK plasmid template and the oligos ...
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bioRxiv - Immunology 2020Quote: ... 1.4 μg PCR products were mixed with 50U T7 RNA polymerase (NEB), 1 U RNase inhibitor (NEB) ...
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bioRxiv - Bioengineering 2020Quote: PCR was performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs). All base editor constructs were assembled using NEBuilder HiFi DNA Assembly (New England Biolabs) ...
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bioRxiv - Molecular Biology 2020Quote: ... Decrosslinked DNA was recovered using the Monarch PCR DNA Cleanup Kit (NEB), eluted in 50 µl of elution buffer and analysed by qPCR using a LightCycler 480 instrument (Roche) ...
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bioRxiv - Genetics 2020Quote: ... The PCR products and the T444T vector were digested with KpnI (NEB) restriction enzyme for all C ...
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bioRxiv - Molecular Biology 2021Quote: Circular cDNA was amplified by PCR using Phusion High-Fidelity enzyme (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... of PCR products of the vector (NEB Phusion High-Fidelity DNA Polymerase) using primers pBEST_GA_1_F ...
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bioRxiv - Developmental Biology 2020Quote: ... the purified PCR product was used for in vitro transcription (NEB, E2040S) followed by reverse transcription (Thermo Fisher ...
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bioRxiv - Molecular Biology 2019Quote: ... Libraries were amplified via 12 cycles of PCR with Phusion polymerase (NEB).
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bioRxiv - Molecular Biology 2020Quote: ... All PCR fragments for cloning were generated using Q5 DNA Polymerase (NEB).
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bioRxiv - Biochemistry 2020Quote: ... PCR reaction products were purified with Monarch DNA Clean-up Kits (NEB) with a binding buffer:sample ratio of 2:1 to remove products smaller than 2kb ...
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bioRxiv - Genetics 2020Quote: ... and verified by restriction enzyme digestion or PCR (Taq DNA Polymerase NEB M0267L ...
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bioRxiv - Genetics 2020Quote: ... Integrated DNA fragments were produced by PCR (Phusion Hot Start Flex NEB M0535L ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR templates for the dsRNA were prepared using T7 Megascript Kit (NEB). S2R+ cells were seeded at the density of 106 cells/ml in serum-free medium and 15 µg/ml of dsRNA was added to the cells ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplification was performed in 25 µL volumes using Q5 polymerase (NEB), 0.5 µM each of the forward and reverse primers as indicated ...
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bioRxiv - Cancer Biology 2019Quote: ... PCRs were done using Q5® High-Fidelity DNA Polymerase (NEB #M0491).
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bioRxiv - Cell Biology 2019Quote: ... The amplified PCR product was treated with DpnI (R0176L; New England Biolabs) for 1h at 37°C ...
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bioRxiv - Genetics 2019Quote: ... PCR was conducted with Q5 Hot Start DNA Polymerase (New England Biolabs) with DNA oligos and gBlocks from Integrated DNA Technologies ...
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bioRxiv - Genetics 2021Quote: ... Samples were PCR amplified using the Phusion High-Fidelity DNA Polymerase (NEB) and an annealing temperature of 68°C over 15 amplification cycles (OG218/OG219) ...
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bioRxiv - Genetics 2020Quote: ... PCR products were first amplified using Phusion High-Fidelity DNA Polymerase (NEB). The avi-tag was sub cloned in pBSSK (EcoRI/Hind III 0.18-kb) ...
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bioRxiv - Developmental Biology 2021Quote: ... Genotyping-PCR was performed with Q5® High-Fidelity DNA Polymerase (NEB) with 98°C initial denaturation for 2 min ...
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bioRxiv - Developmental Biology 2021Quote: ... and amplified using NEBNext® High-Fidelity 2X PCR Master Mix (NEB). The number of cycles was estimated by qPCR ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed using high-fidelity Phusion DNA polymerase (New England Biolabs) except for routine genotyping which was performed using standard Taq DNA polymerase ...
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bioRxiv - Physiology 2021Quote: ... PCR fragments and plasmid backbones were assembled using Gibson assembly (NEB – E2611). For Gateway cloning ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase (New England BioLabs) or PrimeSTAR Max DNA Polymerase (Takara) ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... For all PCR reactions Q5 High-Fidelity 2X Master Mix (NEB M0492L) was used ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs) according to manufacturer’s instructions with primers purchased from Eurofins Genomics ...