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Citations for New England Biolabs :
3901 - 3950 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... PCR assays were performed using the Luna Universal qPCR Master Mix (NEB) in a MasterCycler RealPlex 4 (Eppendorf) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs). Gel fragments were extracted using GeneJet Gel extraction kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... cDNA reactions were purified using the Monarch PCR Purification Kit (NEB #T1030L) and eluted in 10µl elution buffer ...
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bioRxiv - Microbiology 2023Quote: ... All PCR reactions were conducted using the Q5 polymerase (New England BioLabs) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Insert and vector PCR fragments were incubated with Gibson Assembly mastermix (NEB) for 1 h and 50°C ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were assembled using NEBuilder HiFi DNA Assembly (NEB; E2621) following the suppliers’ specifications ...
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bioRxiv - Microbiology 2023Quote: ... All PCRs were performed using Q5 High-Fidelity DNA Polymerase (NEB; M0491). The PCR products were assembled using NEBuilder HiFi DNA Assembly (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... sgRNA was PCR amplified using NEB Q5 Hot Start polymerase (NEB, #M0494L). The product was purified using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... were constructed by PCR (Q5 High-Fidelity DNA Polymerase, NEB cat#M0491S) and ligation or Gibson isothermal assembly (NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting 5’ biotinylated PCR products were digested with BsaI-HF (NEB) to generate four base-pair overhangs ...
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bioRxiv - Cancer Biology 2023Quote: ... All PCR reactions were done with Q5 high-fidelity DNA polymerase (NEB), and PCR products were subsequently purified with DNA clean-up after each PCR and restriction ...
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bioRxiv - Genomics 2023Quote: ... and amplified using NEBnext High-Fidelity 2x PCR Master Mix (NEB, M0541L) and custom indexed primers68 ...
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bioRxiv - Molecular Biology 2023Quote: ... products were purified with the Monarch PCR DNA cleanup kit (NEB, Inc.) and analyzed by endpoint PCR ...
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bioRxiv - Microbiology 2023Quote: ... purification (Monarch PCR & DNA Cleanup Kit, New England Biolabs Cat. No. T1030), and ligation with T4 DNA ligase (New England Biolabs Cat ...
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bioRxiv - Bioengineering 2024Quote: ... 2 and 3 were assembled by PCR assembly using Vent polymerase (NEB), with each reaction consisting of 1 x ThermoPol buffer ...
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bioRxiv - Microbiology 2023Quote: ... The enzyme was removed with the Monarch PCR & DNA Cleanup Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs) for 20 cycles ...
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bioRxiv - Plant Biology 2024Quote: ... PCRs were performed with the Q5® High Fidelity DNA Polymerase (NEB) and specific ...
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bioRxiv - Biochemistry 2024Quote: ... PCR was performed using the Q5 high-fidelity polymerase (New England Biolabs). After each PCR step ...
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bioRxiv - Immunology 2024Quote: ... PCR and Gibson Assembly® Cloning Kit (New England Biolabs, Ipswich, MA). The product was transformed in E ...
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bioRxiv - Immunology 2024Quote: ... Purified PCR product was digested with BamHI-HF and KpnI-HF (NEB) and ligated (Quick Ligase ...
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bioRxiv - Genetics 2024Quote: ... PCR amplification was performed with Q5 High-Fidelity DNA Polymerase (NEB M0491S). Following DPN1 digestion ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were processed according to the Exo-SAP protocol (NEB) and Sanger-sequenced by Azenta Life Services GeneWiz with hisC-ins-f as sequencing primer ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: PCRs were performed using the Phusion DNA Polymerase (New England Biolabs, #M0530S) following manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... The PCR product was incubated with restriction enzyme Fnu4HI (NEB cat. R0178) for >4hrs at 37C and run on a 3% gel ...
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bioRxiv - Physiology 2024Quote: ... We digested the PCR products with AgeI and NotI (New England Biolabs) and cloned them into the first position of the bicistronic vector pCAG-[first position]-2A-GFP ...
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bioRxiv - Genetics 2024Quote: ... PCR reactions were performed with Q5 High-Fidelity DNA Polymerase (NEB, UK). Genomic integration of both constructs were confirmed through PCR and Sanger sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... and amplified using NEBNext® High-Fidelity 2X PCR Master Mix (NEB). The number of cycles was estimated by qPCR ...
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bioRxiv - Cell Biology 2024Quote: For PCR reaction: Taq 2x Master Mix (New England Biolabs Inc, M0270L).
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bioRxiv - Genetics 2024Quote: ... Quantitative PCR was performed using Luna Universal qPCR Master Mix (NEB, M3003L) and a Roche LightCycler 96 machine (Roche) ...
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bioRxiv - Genomics 2024Quote: ... PCR amplification was performed using LongAmp Taq 2X Master Mix (NEB, M0287S) and the Nanopore_T7_IVT_Forward and Reverse primers ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was treated with Exonuclease I (NEB, Ipswich, MA, USA) for 15 min at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... PCR-amplified DNA fragments were assembled by Gibson assembly kit (NEBuilder, NEB) as per manufacturer’s protocol and introduced into DH5α E ...
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bioRxiv - Cell Biology 2024Quote: Monarch® PCR & DNA Cleanup Kit (New England Biolabs, cat. no. T1030S)
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bioRxiv - Genetics 2024Quote: ... the left and right homology arms were amplified by pfusion PCR (NEB) using y w genomic DNA as template and primer pairs Ti897/898 and Ti899/900 ...
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bioRxiv - Biochemistry 2024Quote: ... and digested PCR product by NdeI and XhoI restriction enzymes (NEB, USA) was ligated using T4 DNA ligase (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... Alice Ting’s plasmid (30) via PCR using Q5 high-fidelity (M0492, NEB). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... individual PCR amplicons were produced using Q5 high fidelity polymerase (NEB # M0491S) and isolated from a 0.5X TBE gel by centrifugation of a gel core through an EconoSpin column (Epoch Life Science ...
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bioRxiv - Microbiology 2024Quote: ... Digested products were cleaned using Monarch® PCR & DNA Cleanup Kit (NEB) and incubated with T4 DNA ligase (NEB ...
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bioRxiv - Biochemistry 2024Quote: PCR inserts were amplified with Phusion High-Fidelity DNA Polymerase (M0530L, NEB). Amplification primers were designed with a 30 bp overlap with the linear ends of restriction-digested vectors ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR reactions were performed with the Phusion High Fidelity DNA polymerase (NEB) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed using Luna Universal qPCR Master Mix (M3003, NEB) running on the Light Cycler 96 System (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... 50 µL of PCR product was digested by 1μL of DpnI (NEB) for 2 hours ...
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bioRxiv - Bioengineering 2024Quote: ... These PCR products were digested with ExoCIP (New England Biolabs, Catalog # E1050L) and used as template for a second ...
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bioRxiv - Neuroscience 2024Quote: ... PCR fragments were ligased by HiFi DNA Assembly Master Mix (NEB, E2621L) and into the pCAG-Puro vector by XhoI enzyme site ...
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bioRxiv - Biochemistry 2024Quote: All PCRs were performed with the Q5 High-Fidelity DNA Polymerase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein-coding sequences for cDNA over-expression or co-immunoprecipitation were cloned into pLenti_CMV_cDNA_Flag_SV40_mCherry vector or pLenti_CMV_cDNA_HA_SV40_EGFP vector by PCR and Gibson assembly (NEB, #E2611L). All plasmids were verified by Sanger sequencing.
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bioRxiv - Microbiology 2024Quote: ... the ahpC gene was amplified by PCR with Phusion DNA polymerase (NEB) using C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 25 µL 2X NEBNext high-fidelity PCR master mix (NEB, M0541). To reduce GC/size bias and over amplification of libraries ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed using Q5 high-fidelity DNA polymerase (New England BioLabs) according to the manufacturer’s instructions ...