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Citations for New England Biolabs :
4051 - 4100 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... For generation of the D614G mutant we performed site-directed mutagenesis PCR (NEB) on synthetic viral subgenomic fragments cloned into pUC57 vectors (Thi Nhu Thao et al. ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was cloned into the pMAL-p5x vector (New England Biolabs) using the XmnI and BamHI restriction sites (underlined ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... PCR fragments were purified using Monarch DNA Gel Extraction Kit (New England Biolabs), and were digested with EcoRI and BamHI (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... An aliquot of each PCR product was digested with HaeIII (New England Biolabs) at 37°C for 60 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... and then amplified with NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L). Libraries were sequenced on HiSeq 125bp paired-end platform.
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bioRxiv - Developmental Biology 2021Quote: ... Grb10a was amplified from cDNA by high specificity PCR (NEB Q5 Hot Start) with primers flanking open reading frame (Table 1) ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR reaction was DpnI digested (New England Biolabs, 37°C, 1 h) and column purified (Macherey-Nagel ...
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bioRxiv - Microbiology 2021Quote: ... PCR reactions were performed using Phusion High Fidelity DNA polymerase (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was performed with PCR products and pCFD4 BbsI (New England Biolabs, Cat. # R0539L) digested vector ...
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bioRxiv - Bioengineering 2022Quote: ... PCR fragments were then purified using a gel extraction kit (New England Biolabs) and subjected to sanger sequencing ...
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bioRxiv - Biochemistry 2022Quote: Genotyping was done by DNA PCR using Q5 (New England Biolabs cat # M0492L) and confirmed the presence of the expression cassettes.
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bioRxiv - Synthetic Biology 2022Quote: ... and amplified by PCR using Q5 hot start high-fidelity polymerase (NEB #M0494S) and primers with appropriate overhangs ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCRs were performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs (NEB), Cat#M0491S/L ...
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bioRxiv - Bioengineering 2022Quote: ... PCR product was purified and digested with EcoRI and SacI (New England Biolabs). The adhB_pdc operon that encodes AdhB alcohol dehydrogenase and Pdc pyruvate decarboxylase from Zymomonas mobilis with synthetic ribosome binding sites was synthesized by Genewiz ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments were then concentrated on columns (Monarch PCR & DNA Cleanup Kit, NEB). The size of the DNA fragments generated (150-400 bp ...
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bioRxiv - Microbiology 2019Quote: ... PCR amplifications for cloning purposes were performed with Q5 polymerase (New England Biolabs) while DNA Amplitools Master Mix was used for diagnosis PCRs ...
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bioRxiv - Genetics 2019Quote: ... and 10 µL 2X Hot Start Taq PCR Master Mix (New England Biolabs). The cycling conditions in the Bio-Rad T100™ Thermal Cycler were ...
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bioRxiv - Genetics 2019Quote: ... and 12.5 µL 2X Hot Start Taq PCR Master Mix (New England Biolabs). Samples were run on a Bio-Rad T100™ Thermal Cycler and cycled for 5 minutes at 95°C ...
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bioRxiv - Genetics 2019Quote: ... and 10 µL 2X Hot Start Taq PCR Master Mix (New England Biolabs). Samples were loaded into a Biorad T100™ Thermal Cycler for 3 minutes at 95°C ...
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bioRxiv - Genomics 2019Quote: ... in a 50⍰ μl reaction with 2× PCR master mix (NEB cat. M0541). PCR cycled as ...
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bioRxiv - Cell Biology 2019Quote: ... 25 ng of the PCR product was ligated with T4 DNA Ligase (NEB) in a final volume of 10 μL for 1h at RT ...
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bioRxiv - Biochemistry 2019Quote: ... PCR was performed using Q5 Host Start High- Fidelity 2× Master Mix (NEB), and 150 ng Genomic DNA was chosen as the template ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were assembled into an intact plasmid using NEBuilder HiFi mix (NEB). Plasmids were transformed into E ...
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bioRxiv - Genomics 2019Quote: ... Clones were screened by genomic PCR using LongAmp Taq Polymerase (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... PCR reactions were performed using Q5® High-Fidelity 2x Master Mix (NEB) for cloning and sequencing otherwise DreamTaq Green PCR Master Mix 2x (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs, NEB). Mix 10 ng of ssDNA pool (5 uL ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs, NEB). Mix 10 ng of ssDNA pool (5 uL ...
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bioRxiv - Cell Biology 2019Quote: ... and Tfcp2l1 (NM_023755) were amplified by PCR with Q5 DNA polymerase (NEB, M0493) and primers containing an EcoRI restriction site and subsequently subcloned into the unique EcoRI restriction site in pBS31 [48] to generatepBS31–TetON-Esrrb ...
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bioRxiv - Microbiology 2019Quote: ... Stop codons were inserted by PCR using the high-fidelity Q5 polymerase (NEB) per suggested protocols with the following cycling conditions ...
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bioRxiv - Microbiology 2020Quote: Polymerase chain reaction (PCR) was performed using Q5 DNA polymerase (New England Biolabs). For cloning ...
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bioRxiv - Immunology 2019Quote: ... and enrichment PCR performed with NEBNext Ultra Q5 Master Mix (New England BioLabs). Samples were pooled in equal proportions and size-selected using 0.6x followed by 0.2x of AMPure XP beads (Beckman Coulter ...
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bioRxiv - Biophysics 2019Quote: ... A standard PCR was performed using Taq DNA polymerase in ThermoPol buffer (NEB) at an annealing temperature of 60°C and elongation temperature of 68°C ...
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bioRxiv - Molecular Biology 2021Quote: ... then ligated with the phosphorylated PCR fragments by T4 DNA Ligase (NEB, M0202S). Inserts were analyzed by Sanger sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... All PCR amplification steps used the high fidelity Q5 Polymerase (New England Biolabs) and primers from Integrated DNA Technology (Coralville ...
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bioRxiv - Biochemistry 2020Quote: PCR was performed using Phusion HF polymerase purchased from New England Biolabs (NEB). T4 polynucleotide kinase (T4 PNK ...
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bioRxiv - Cancer Biology 2019Quote: ... The PCR amplicons were subjected to restriction digestion using BsaXI (New England Biolabs) for 60 minutes at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... The PCR was performed with Q5 High-Fidelity DNA Polymerase (New England Biolabs), temperatures were as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were converted to RNA using the T7 RNA Transcription Kit (NEB) and purified with RNEasy Kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... and enrichment PCR performed with NEBNext Ultra Q5 Master Mix (New England BioLabs). Samples were pooled in equal proportions and size-selected using 0.6x then 0.2x AMPure beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2019Quote: ... and the GFP PCR was digested with AatII and BglII (New England Biolabs). The two fragments were then ligated together (via the AatII digest site ...
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bioRxiv - Genetics 2019Quote: ... PCR was performed with Phusion® High-Fidelity DNA Polymerase (New England Biolabs) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Each 100 μl PCR reaction contained 1x Phusion HF reaction Buffer (NEB, #B0518S), 3% v/v DMSO ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were digested with 20 units of XmnI and XcmI respectively (NEB) to distinguish cleavable mutant from un-cleavable wild-type amplicons ...
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bioRxiv - Genomics 2021Quote: ... the PCR reactions were treated with 200 U/ml of Exonuclease I (NEB) for 1 h at 37°C ...
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bioRxiv - Genetics 2021Quote: ... Mutagenesis PCR product was then digested overnight using DpnI (New England Biolabs, R0176) followed by bacterial transformation into competent cells ...
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bioRxiv - Genetics 2021Quote: ... PCR was limited to 18 cycles using Phusion polymerase (New England Biolabs, M0530). Mutagenesis PCR product was then digested overnight using DpnI (New England Biolabs ...
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bioRxiv - Plant Biology 2020Quote: ... Gel-purified PCR products were A-tailed using Taq polymerase (New England Biolabs) for 30 min at 72 °C ...
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bioRxiv - Genomics 2021Quote: ... the PCR reactions were treated with 200 U/mL of Exonuclease I (NEB) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and assembled into PCR amplified pFastBac vector by NEBuilder HiFi DNA assembly (NEB). CAMSAP2 mutant constructs were generated by inserting each mouse CAMSAP2 partial PCR fragment flanked by a TEV protease recognition linker (ENLYFQGSSGSSG ...
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bioRxiv - Microbiology 2021Quote: ... PCR reactions were carried out using Q5 High-Fidelity polymerase (New England Biolabs) and oligomers used for amplifications listed in Table S1 ...