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Citations for New England Biolabs :
4001 - 4050 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... PCR conditions: 0.02 U/μL Phusion High-Fidelity DNA Polymerase (New England Biolabs), 1X Phusion HF buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Inserts were amplified by PCR using Q5 2x Hotstart mastermix (NEB, Ipswich, MA), and purified by agarose gel purification and extraction (primers ...
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bioRxiv - Neuroscience 2020Quote: ... Libraries were prepared with NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541L), following standard protocol ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed using 2x LongAmp Taq Master Mix (New England Biolabs, M0287S) to select full- length transcripts followed by a second purification step using Agencourt beads (as described above) ...
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bioRxiv - Neuroscience 2020Quote: ... RPF libraries were PCR amplified with Phusion High-Fidelity DNA Polymerase (NEB M0530A) and the primer set DP5-PE/SP3-PE ...
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bioRxiv - Microbiology 2021Quote: ... PCR reactions were carried out using Q5 High-Fidelity DNA polymerase (NEB, UK).
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bioRxiv - Cancer Biology 2021Quote: ... Genotyping PCRs were performed with Phusion High-Fidelity DNA Polymerase (NEB; Cat#: M0530S), using primers surrounding the genomic target site.
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bioRxiv - Cancer Biology 2020Quote: ... PCR amplicons were then digested with PmeI restriction enzyme (New England BioLabs #R0560S) in OneTaq buffer for 1h at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... 10 ng of purified PCR products were incubated with I-SceI endonuclease (NEB) according to manufacturer’s instruction ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA sequences were PCR amplified using Q5 High Fidelity Polymerase (New England Biolabs) and cloned into SR54 proGL2:EYFP cleaved with SalI and KpnI using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... 50 ng of the PCR product was ligated with T4 DNA ligase (NEB) in a final volume of 10 μL overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The enriched DNA library was amplificated by PCR using Q5 DNA polymerase (NEB). After size-selection with the AMPure XP beads (Beckman ...
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bioRxiv - Microbiology 2020Quote: ... The amplified PCR product was digested by MluI and NheI restriction enzymes (NEB), and was ligated into the pVSV-FL+(2 ...
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bioRxiv - Microbiology 2020Quote: PCR was carried out according to the manufacturer’s protocols using Phusion polymerase (NEB), or Advantage 2 Polymerase mix (Takara Bio ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified and in vitro transcribed using T7 HiScribe kit (NEB). Transcribed RNA products were run on a 5% acrylamide TBE-urea gel and visualized with 0.02% methylene blue in 1x TBE ...
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bioRxiv - Microbiology 2021Quote: ... PCRs to generate specific gRNAs were performed with Q5 polymerase (New England Biolabs) while PCRs to generate specific knock-in constructs (mAID fusions and epitope tagging ...
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bioRxiv - Microbiology 2021Quote: ... pET-28a-rbpB was cloned using Q5 PCR amplification and NEBuilder assembly (NEB) in E ...
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bioRxiv - Microbiology 2021Quote: BsNadDBs was PCR amplified with Phusion High-Fidelity DNA polymerase (New England BioLabs) from Bacillus subtilis 168 genomic DNA using primers BsNadD_Fw and BsNadD_Rv-XhoI digested with NdeI/XhoI and ligated into the matching sites of pET28b (Novagen) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The resulting PCR products were self-assembled using T4 ligase (New England Biolabs) to obtain the desired SSGM plasmid libraries ...
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bioRxiv - Immunology 2021Quote: ... PCR products were generated using Phusion High-Fidelity DNA Polymerase (New England Biolabs) or Herculase II Fusion DNA Polymerase (Agilent Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was purified using the Monarch PCR and DNA cleanup kit (NEB) to remove unincorporated dATP ...
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bioRxiv - Microbiology 2021Quote: ... The resultant PCR fragment was digested with XhoI and HindIII restriction enzymes (Biolabs) then cloned into pBBRMCS1 ...
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bioRxiv - Microbiology 2021Quote: ... was amplified from genomic DNA by PCR using Q5 polymerase (New England Biolabs) with primers ML4212/ML4213 and cloned into the DHFR-TetO7Sag4 plasmid ...
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bioRxiv - Plant Biology 2020Quote: ... a fragment of MIST1 was PCR amplified using Q5 DNA polymerase (NEB USA) with MIST1_PhasiRNA_ProbeF and MIST1_PhasiRNA_ProbeR ...
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bioRxiv - Microbiology 2021Quote: ... for PCR amplification and the Gibson Assembly Master Mix (New England Biolabs, NEB) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... for PCR amplification and the Gibson Assembly Master Mix (New England Biolabs, NEB) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR round 1 and 2 were performed using Q5-High Fidelity polymerase (NEB) for 6 (FS231 and FS232 ...
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bioRxiv - Microbiology 2019Quote: All the DNA fragments were PCR-amplified with Phusion polymerase (New England BioLabs). PCR products were gel purified ...
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bioRxiv - Microbiology 2019Quote: ... PCR amplifications were performed using Q5 High fidelity DNA polymerase (New England Biolabs) under the following conditions ...
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bioRxiv - Microbiology 2020Quote: ... All PCR-amplifications were performed using Phusion High-Fidelity DNA Polymerase (NEB, M0530S).
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bioRxiv - Microbiology 2021Quote: All PCRs were carried out using Phusion High Fidelity Polymerase (NEB Labs, M0530). To begin with we PCR amplified ZeoR cassette flanked by loxP sites using pMSG360zeo as template (50 ...
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bioRxiv - Biochemistry 2022Quote: ... Linearized vector and PCR fragments were combined using ligation independent cloning (LIC, NEB). Reactions were transformed into chemically competent E ...
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bioRxiv - Molecular Biology 2022Quote: ... The remaining circularized products were amplified by PCR using Phusion polymerase (#M0530L, NEB). In a pilot experiments ...
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bioRxiv - Molecular Biology 2022Quote: ... agarose gel and PCR clean ups were performed using Monarch DNA kits (NEB). Plasmid DNA constructs were confirmed by sequencing on the ABI 3130xl capillary machine (Applied Biosystems ...
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bioRxiv - Developmental Biology 2022Quote: ... using the NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs #M0451S) with Nextera DNA CD Indexes (Illumina #20015882) ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplification was performed using Phusion High-Fidelity DNA Polymerase (New England Biolabs) and the PCR products were sequenced by Genewiz ...
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bioRxiv - Neuroscience 2022Quote: ... 25 μl NEBNext High-Fidelity 2× PCR Master Mix (New England Biolabs, M0541S), 9 μl of unique ...
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bioRxiv - Molecular Biology 2022Quote: ... and all PCR reactions were performed with the Phusion DNA polymerase (NEB M0530S). All restriction enzymes were purchased from NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100–500 fmol of library was generated with Q5 PCR mix (NEB).
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using Monarch DNA Gel Extraction Kit (New England Biolabs). Both the vector and the complete insert were digested by BsmBI (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using Monarch DNA Gel Extraction Kit (New England Biolabs). Both the vector and the complete insert were digested by BsmBI (New England Biolabs ...
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bioRxiv - Genetics 2022Quote: ... PCRs were performed on above cDNAs with Q5 High-Fidelity DNA Polymerase (NEB) using 484-qMag-both-F as forward primer and 242-Gam-locus-R (365bp amplicon ...
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bioRxiv - Microbiology 2022Quote: ... and PCR products were recircularised using KLD enzyme blend (New England Biolabs (UK)) ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR amplification was performed using Phusion High-Fidelity DNA Polymerase (New England BioLabs) using the following primers ...
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bioRxiv - Genetics 2022Quote: Conversion tracts were PCR amplified using OneTaq 2x Master Mix (New England Biolabs). Noncrossover recombination products were amplified using forward primer DLO822 (5’-ATTTTAACCCTTCGGGGTACG-3’ ...
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bioRxiv - Immunology 2022Quote: ... TCRs were then amplified by PCR (20 cycles with the Phusion from NEB) with a single primer pair binding to the constant region and the adapter linked to the TRAV/TRBV primers added during the reverse transcription ...
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bioRxiv - Biophysics 2022Quote: ... for PCR amplification and isothermal assembly using 2X Gibson master mix (NEB E2611) before transformation into NEB Stable or 5-alpha chemically competent cells.
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bioRxiv - Biophysics 2022Quote: ... The PCR amplification was performed with Q5 High- Fidelity DNA Polymerase (NEB, USA). Using Gibson Assembly Master Mix (NEB ...
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bioRxiv - Microbiology 2022Quote: ... Single-round PCR was performed using Q5 high-fidelity DNA polymerase (NEB; M0491) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... PCR products recombined using the NEBuilder HiFi DNA assembly kit (New England Biolabs). All constructs were verified by sequencing.