Labshake search
Citations for New England Biolabs :
2851 - 2900 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... we amplified the targeted gene regions by PCR (NEB Next High-Fidelity 2xPCR Master Mix; New England Biolabs) using primers (Supplementary Table 2 ...
-
bioRxiv - Developmental Biology 2024Quote: ... The cDNA sequence for dld was cloned from isolated cDNA using Phusion High Fidelity Polymerase (New England Biolabs) and cloned into a pME vector digested with EcoRI using Gibson Assembly and the following primers for homology ...
-
bioRxiv - Genetics 2023Quote: ... DNA was amplified with Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs; catalog no. M0494S). For the 1st stage PCR ...
-
bioRxiv - Microbiology 2024Quote: ... All PCR products were amplified using Q5-High Fidelity DNA polymerase according to the manufacturer (New England Biolabs).
-
bioRxiv - Synthetic Biology 2023Quote: ... The ligations were transformed into high efficiency (1-3 × 109 CFU/μg pUC19 DNA) competent cells (NEB C3040). The transformations were serially diluted and plated on LB with ampicillin (100 μg/ml) ...
-
bioRxiv - Genetics 2023Quote: ... the primary probes were amplified using Phusion® High-Fidelity PCR Master Mix with HF Buffer (M0531S, NEB) by monitoring the amplification on a qPCR machine (CFX Connect ...
-
bioRxiv - Systems Biology 2023Quote: IFRD1 splicing variants were amplified using Q5 High Fidelity Polymerase (New England Biolabs, Ipswich, MA, USA, Cat. # M0491S) on an AriaMX Real-Time PCR System (Agilent ...
-
bioRxiv - Genetics 2023Quote: ... The T7 promoter and mtdTomato CDS were amplified from pmrPTRE-AAV using PTRE_floxed_F/R and Phusion High-Fidelity PCR Master Mix (NEB). NotI and SalI sites added by the primers were used to subclone this amplicon into pRM1506_TMM432 ...
-
bioRxiv - Biochemistry 2022Quote: ... all samples were amplified by a 8-cycle PCR-program using Phusion High-Fidelity DNA Polymerase (NEB #M0530L) using primers 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3’and 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’ ...
-
bioRxiv - Developmental Biology 2022Quote: ... Patient-derived variants were created by site-directed mutagenesis using Q5 High Fidelity Master Mix (NEB Catalog #M0492L), T4 polynucleotide kinase (NEB Catalog# M0201L ...
-
bioRxiv - Molecular Biology 2023Quote: ... MYH7 fragment containing mutation was amplified by PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) and 500 nM forward and reverse primers (Table 3) ...
-
bioRxiv - Molecular Biology 2022Quote: ... For off-target analysis and HiBiT integration Q5 Hot Start High-Fidelity 2x Master Mix (New England Biolabs) was used in 15 μL reactions with 500 nM of target-specific primers and the following cycling conditions for off-target analysis ...
-
bioRxiv - Bioengineering 2023Quote: ... The coding sequence of the libraries was amplified using the Q5 Hot Start High-Fidelity DNA Polymerase (NEB) and the PCR amplicons were separated from primer dimers on a 0.5x TAE 1 % agarose gel ...
-
bioRxiv - Plant Biology 2023Quote: ... Target loci were amplified using a proof-reading polymerase (Q5® High-Fidelity DNA Polymerase, New England Biolabs, Ipswich ...
-
bioRxiv - Microbiology 2022Quote: ... All site-directed mutagenesis with IMCs was done using the Q5 high-fidelity DNA polymerase (New England Biolabs) and One Shot Stbl3 Chemically Competent E ...
-
bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were conducted using the Q5® High-Fidelity DNA polymerase system (New England BioLabs, Ipswich, MA); 5 μL 5X Q5 Reaction Buffer,0.25 μL Q5 High Fidelity DNA Polymerase ...
-
bioRxiv - Genomics 2023Quote: ... the oligo pool for each library was amplified with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541L) and the following primers ...
-
bioRxiv - Biochemistry 2023Quote: ... of the human PNKP (gene accession#NM_007254.4) was amplified using the Q5 hot start high fidelity DNA polymerase (New England Biolabs), with HEK293 genomic DNA as a template ...
-
bioRxiv - Genomics 2023Quote: ... were amplified by polymerase chain reaction (PCR) with the Phusion High-fidelity DNA Polymerase (New England Biolabs #M0530L), then both amplicons were assembled by Gibson assembly to produce the desired CRISPRoff-mScarletI plasmid.
-
bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was carried out with 1.5 mM (high concentration) or 0.1 mM (limiting concentration) dNTP mix and 2.5 U AMV-RT (NEB, M0277S) and 1 hour incubation at 42°C ...
-
bioRxiv - Systems Biology 2023Quote: The pool of guide arrays was PCR amplified using NEBNext® High-Fidelity 2X PCR Master Mix (NEB) using 196 ng of starting template per 50 μl reaction using forward and reverse primers:
-
bioRxiv - Genetics 2023Quote: ... Pooled DNA libraries were amplified in 50 µl reactions with 1x NEBNext High Fidelity PCR Master Mix (NEB) and 12.5 pmol PCR primers containing complementary sequences for adapter-ligated DNA ...
-
bioRxiv - Developmental Biology 2023Quote: ... and beads resuspended in a 50-µl PCR mix containing 1x NEBNext High-Fidelity PCR Master Mix (NEB) and 0.5 µM of Nextera Ad1_noMX and Ad2.X primers ...
-
bioRxiv - Microbiology 2023Quote: ... PCR reactions were undertaken using sporocarp extractions using the PHusion High-Fidelity PCR Master Mix (New England Biolabs). Libraries were generated using the TruSeq DNA PCR-Free Prep Kit (Illumina ...
-
bioRxiv - Microbiology 2023Quote: ... The gene of interest was amplified using primers described using either Q5 High-Fidelity DNA polymerase (NEB; M0491S) or Platinum Taq DNA polymerase High Fidelity (ThermoFisher Scientific ...
-
bioRxiv - Bioengineering 2023Quote: ... amplicons were generally obtained using high-fidelity Q5 Hot Start (2X Master Mix) or Phusion polymerases from NEB; otherwise ...
-
bioRxiv - Plant Biology 2023Quote: ... Promoters and coding sequences were commercially synthesized (Eurofins) or PCR-amplified using the Q5 high fidelity polymerase (NEB) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... The sequence corresponding to residues 1-239 was amplified by PCR using Q5 High-Fidelity DNA Polymerase (NEB), cut with restriction enzymes NdeI (NEB ...
-
bioRxiv - Bioengineering 2023Quote: ... was obtained and PCR amplified using Q5 Hot Start high fidelity DNA polymerase (New England Biolabs, Catalog #M0493S) from position 376-1125 ...
-
bioRxiv - Genomics 2023Quote: Libraries were prepared by combining transposed DNA with NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs) and 1.25µM indexing primers (Ad1_noMX primer and Ad2.x indexing primer 45 or IDT for Illumina dual index primers (Illumina ...
-
A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... using the primers outlined in Table S1 and Q5 Hot-start high fidelity polymerase (New England Biolabs M0494S). PCR products were extracted from standard agarose gels using the QIAquick Gel Extraction Kit (Qiagen 28704 ...
-
bioRxiv - Cancer Biology 2023Quote: ... JH4 intron sequences were amplified from genomic DNA by PCR with Phusion High-Fidelity DNA polymerase (NEB; M0530S) using JH4 forward primer and JH4 reverse primer ...
-
bioRxiv - Microbiology 2023Quote: ... That primer was used with primer 1092 for PCR with Q5 DNA polymerase with high-GC buffer (NEB). The raw PCR product was used for blunt end ligation as above.
-
bioRxiv - Biochemistry 2023Quote: ... Polymerase chain reactions (PCRs) were conducted with Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, USA), PCR purifications with the Monarch® PCR & DNA cleanup kit (New England Biolabs ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... VK7 cDNA was used as template for PCR amplification using Phusion High-Fidelity DNA Polymerase (New England Biolabs), following manufacturer’s instructions ...
-
bioRxiv - Physiology 2023Quote: ... muGFP and homology arms were PCR-amplified using Q5 High-Fidelity 2× Master Mix (New England BioLabs, M0492L). Primers for PCR were designed using the NEBuilder Assembly Tool ...
-
bioRxiv - Microbiology 2023Quote: Five POWV-LI9 fragments were amplified from viral cDNA (Figure 1A,B) using high-fidelity Phusion polymerase (NEB) and corresponding paired primers (Table S2 ...
-
bioRxiv - Microbiology 2023Quote: ... Amplification of the complete ORF was performed using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs), including high GC Enhancer ...
-
bioRxiv - Systems Biology 2023Quote: ... with 1.5 µg undigested genomic DNA per PCR reaction using Q5 HotStart High Fidelity Polymerase (New England Biolabs). Resulting PCR product from multiple reactions per sample were pooled ...
-
bioRxiv - Developmental Biology 2023Quote: ... PCR was performed for 12 cycles using NEBnext High-Fidelity 2X PCR Master Mix (New England Biolabs, M0541L) (41) ...
-
bioRxiv - Microbiology 2024Quote: Polymerase chain reactions (PCRs) for cloning purposes were performed using the high fidelity Phusion DNA Polymerase (NEB, France). PCR products were purified with the NucleoSpin PCR Clean Up kit (Macherey Nagel ...
-
bioRxiv - Molecular Biology 2023Quote: ... Templates for in vitro transcription were generated by PCR amplification (Q5 High-Fidelity DNA Polymerase, New England Biolabs) from plasmids using a T7 extended primer (5’-CCTAATACGACTCACTATAGGGAG-3’ 85) ...
-
bioRxiv - Cancer Biology 2023Quote: ... PCR amplification at the α-tubulin locus was done using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) with a 30 s extension time ...
-
bioRxiv - Synthetic Biology 2023Quote: ... All reporters were constructed by site-directed mutagenesis via PCR sing Q5® High-Fidelity DNA Polymerase (NEB) to create overlaps forming the Fetch sequence followed by Gibson assembly and transformation into DH10β cells ...
-
bioRxiv - Cell Biology 2023Quote: ... Site-directed mutagenesis of TBK1 and Rab7 genes was performed using Q5 High-Fidelity 2X Master Mix (NEB). Plasmids are listed in Table 3 and were confirmed by Sanger Sequencing (Keck DNA Sequencing facility ...
-
bioRxiv - Plant Biology 2024Quote: ... PCR fragments used for cloning were obtained using Q5 high-fidelity DNA polymerase (New England Biolabs, Frankfurt, Germany). All PCR-based constructs were sequenced ...
-
bioRxiv - Biophysics 2024Quote: ... dsDNA templates were prepared by PCR amplification of WT_bPTC and Sh_bPTC ssDNA oligos using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: The DASHed library was PCR amplified with NEBNext Ultra II Q5 High-Fidelity 2X Master Mix (NEB, M0544) and 0.5 uM PCR primers with unique dual index using the two-step PCR cycles:
-
bioRxiv - Synthetic Biology 2024Quote: All PCR amplifications for plasmid construction and cloning were performed using Q5® High-Fidelity DNA Polymerase (NEB), followed by purification with Monarch® PCR & DNA CleanupKit (NEB) ...
-
bioRxiv - Genetics 2024Quote: We amplified the oligo pool using five cycles of PCR with Phusion High-Fidelity polymerase (New England Biolabs) and primer set KC_1 (Table S4) ...