Labshake search
Citations for New England Biolabs :
2001 - 2050 of 2293 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: The resulting PCR product of the PCR amplifying the wildtype hemA with added Kpnl sites and the recipient plasmid pHSG396 were digested with the restriction enzymes Kpn1 (New England Biolabs, US). PCR clean-up was then performed using the Monarch PCR and DNA Clean-up Kit following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the sgRNA cassette from lentiCRISPRv2 was removed by digestion with KpnI and EcoRI and the plasmid re-ligated using annealed repair oligo pair 1 (see Table S3) and Quick Ligation™ Kit (M2200L, NEB). Next ...
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bioRxiv - Plant Biology 2021Quote: Entry vectors for Golden Gate cloning were made by digesting the Greengate Kit [56] entry vector plasmids (PGGA000, PGGB000, PGGC000, PGGD000, PGGE000, PGGF000) with BSA1-HF V2 (New England Biolabs Inc.). Bladderwort sequences were synthesized (IDT ...
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bioRxiv - Cell Biology 2021Quote: ... pYX142 and pNH605 plasmids used in this study was performed by Gibson Assembly with the HiFi DNA Assembly Master Mix (New England Biolabs, #E2621L) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... was performed using 10ng of His tagged PTP-PEST (WT) plasmid as template and followed by Dpn1 (New England Biolabs, UK) digestion for 2 hours at 37°C ...
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bioRxiv - Genomics 2020Quote: ... A 2µ -URA plasmid was constructed from three DNA fragments using HiFi DNA assembly Master Mix (New England Biolabs, Ipswich, MA) for cloning ...
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bioRxiv - Molecular Biology 2022Quote: ... The codon random mRFP library (15 μl) was ligated into 30 ng linear plasmid with 400 units of T4 ligase (NEB, M0202) and 2 μl T4 Ligase Buffer (NEB ...
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bioRxiv - Microbiology 2022Quote: ... All CRISPR/Cas9 plasmids designed and used in this study were created by using Q5 Site-Directed Mutagenesis Kit (NEB-E0554S) based on the previously described CRISPR/Cas9 plasmid [58] as primary scaffold ...
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bioRxiv - Microbiology 2022Quote: ... The mcr-1 gene along with its natural promoter was PCR-amplified from the natural PN16 (IncI2) plasmid using Q5® High-Fidelity DNA Polymerase (New England BioLabs). The amplified and purified mcr-1 fragment was cloned into PCR-amplified pSEVA121 backbone using NEBuilder® HiFi DNA Assembly Master Mix according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: mRNA synthesis via in vitro transcription was performed using linearized plasmid DNA using High Scribe T7 Polymerase HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs): 100 mM NTPs ...
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bioRxiv - Molecular Biology 2022Quote: pGEMHE plasmid constructs (Supplementary Table 2A) were linearized and 5’-capped mRNA was synthesized with T7 polymerase (NEB HiScribeT7 ARCA kit) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The spike plasmids were linearized by restriction enzymes and transcribed to mRNA by in vitro T7 RNA polymerase (NEB, Cat # E2060S) as previously described7,8.
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bioRxiv - Immunology 2022Quote: ... the p300 core domain from plasmid pLV-dCas9-p300-P2A-PuroR67 was switched to a VPRmini49-FLAG with Gibson Assembly after BAMHI-HF (NEB, R3136S) digestion to generate pLV-dCas9-VPRmini ...
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bioRxiv - Synthetic Biology 2019Quote: ... The p416-loxP-KmR-TEFmut6-yECitrine plasmid was linearized with KpnI and dephosphorylated with Antarctic Phosphatase (New England Biolabs, MA, USA), and ligated with the mCherry expression cassette by T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... we used the aforementioned primers M13/pUC Forward and L4440 Reverse and the isolated plasmid (template) in a OneTaq® 2X Master Mix with Standard Buffer (NEB) reaction as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2019Quote: Production of new plasmids for this study was accomplished by standard restriction-ligation or NEBuilder HiFi DNA Assembly (New England Biolabs E5520S), typically using Clontech pEGFP-C1 and -N1 backbones or their derivatives ...
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bioRxiv - Molecular Biology 2019Quote: ... 150 ng of double stranded gBlock® template or 2 µg of plasmid template was transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (New England BioLabs®). At the end of the reaction ...
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bioRxiv - Synthetic Biology 2019Quote: The adipic acid biosensing plasmid (JBx_101898) was assembled of 4 DNA parts by NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, MA): First ...
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bioRxiv - Synthetic Biology 2020Quote: The plasmid pGEMM7 was assembled from seven PCR fragments containing independent transcriptional cassettes and the plasmid backbone of pUC19 (New England Biolabs, USA) (Supplementary Table 1) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Peptide tags were cloned into pFA6a-KANMX6 by amplifying the plasmid backbone and inserting gBlocks (Integrated DNA Technologies) using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). Except where otherwise noted ...
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bioRxiv - Microbiology 2019Quote: ... All plasmids were constructed via Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Inc., Cat. No. E2621X) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... PCR amplification of the relevant regions was performed using Thermoscientific™ Phire™ Hot Start II DNA Polymerase on plasmids isolated from stock cultures with the QIAprep® Spin Miniprep Kit followed by linearization with appropriate restriction enzymes (NEB).
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bioRxiv - Microbiology 2020Quote: ... was linerized by inverse PCR with primers 13 and 14 and the duplex oligo was ligated to the linearized plasmid in a 2:1 insert:vector molar ratio using NEBuilder Hifi Assembly Master Mix (New England Biolabs, Ipswich, Massachusetts) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: A digoxin-labeled human H19X probe was transcribed in situ from a linear template plasmid by T7 RNA polymerase (NEB, UK) with Digoxin-labeled Uridine (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... a biotin-labeled H19X RNA molecule and its antisense RNA molecule (X91H) were transcribed in vitro from linear plasmid templates by T7 RNA polymerase (NEB, UK) with Biotin pre-labeled Uridine (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... Both purified genes and plasmids were digested with Nde I and Not I prior to ligation using the T4 DNA ligase (New England Biolabs, UK). The recombinant plasmids pET-hGFAT2 (without tag ...
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bioRxiv - Microbiology 2019Quote: ... was cloned into pNL4-3 plasmid following digestion with BssHII and SalI (New England Biolab, USA) and ligation using T4 DNA ligase (New England Biolabs, USA). The chimeric viruses were produced by transient transfection of the plasmids into the 293T cell line using FuGene HD and harvested 72 hours later by a collection of the cell-free supernatant cleared by centrifugation and stored in aliquots at −80°C.
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CRISpy-pop: a web tool for designing CRISPR/Cas9-driven genetic modifications in diverse populationsbioRxiv - Genetics 2020Quote: ... was first cloned into the pKOPIS plasmid (Kuang et al. 2018) using the NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) (Hsieh 2018) ...
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bioRxiv - Cell Biology 2021Quote: ... containing TauRD-I260C for fluorescent labeling were constructed by mutagenesis of the pHUE-TauRD (P301L/V337M) plasmid using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Evolutionary Biology 2020Quote: ... The ZEB2 ORF was amplified with primers ZEB2_IndOE_F & _R and tagged with GFP-Flag by Gibson assembly into the pT2-CAG-MCS-2A-Puro plasmid linearized by restriction digestion with EcoRI (NEB, R3101S) and AgeI (NEB ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 pg of lhx1a:DsRed/pI-SceI plasmid DNA was injected into 1-cell stage embryos along with the I-SceI restriction enzyme (NEB, R0694S). These injected embryos were raised to adulthood and screened for DsRed expression in known lhx1a expression domains and isolated a stable Tg(lhx1a:DsRed ...
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bioRxiv - Biochemistry 2021Quote: ... Transcription templates were amplified from the plasmids by PCR and and transcribed in vitro with the HiScribe T7 High Yield RNA synthesis kit (New England Biolabs Inc.). To obtain sufficient amounts of mRNA ...
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bioRxiv - Cancer Biology 2020Quote: ... cells we first created the pHAGE-empty plasmid (pAS4940) by excising the ERBB2 coding sequence from pHAGE-ERBB2 (addgene #116734) using Xho1 (NEB, R0146S) followed by re-ligation of the vector ...
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bioRxiv - Cell Biology 2020Quote: ... Two missense point mutations present in the initial plasmid (c1411t and c2671t) were corrected using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S) according to manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: ... The seven PCR products were assembled into the pCC1-F567-mNG plasmid that were pre-linearized with NheI and XhoI by using the NEBuilder® HiFi DNA Assembly kit (NEB) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: ... The 3-part assembly reaction (plasmid-promoter-insert) was performed using the Gibson assembly master mix 2x (New England Biolabs #E2611S), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme digest of 500 ng of plasmid DNA extracted from 190693 and 169757 was performed with PpuMI and XhoI (both New England Biolabs, USA) and immediately run on a 1% agarose gel following incubation for 1 hour at 37°C.
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bioRxiv - Molecular Biology 2021Quote: Site-directed mutagenesis of the AP-1 binding sites in the distal enhancer reporter plasmids were performed using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs). Primer pairs 5’-CCATAATGTGgggCTATACTAAATTTCATCTTC-3’ and 5’-CTAAATCCACTTAGAAAAAACAATC-3’ ...
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bioRxiv - Genetics 2020Quote: ... The digestion product was resolved on a 0.7% agarose gel and the plasmid backbone was purified using the Monarch gel purification kit (NEB, Cat# T1020S). The ‘T2A-GFP-WPRE’ sequence was inserted into the digested backbone using the Gibson Assembly kit (SGI ...
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bioRxiv - Genomics 2021Quote: ... Gibson Assembly was performed using the amplified plasmid backbone and a synthesized DNA fragment “GibsonBC4” according to the manufacturer’s protocol (NEB cat# E2611L). Barcoded plasmid library DNA was purified using the Zymo Clean and Concentrate-5 (Zymo Research cat# D4014 ...
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bioRxiv - Molecular Biology 2021Quote: ... 500bp upstream and 500bp downstream sequence from start codon was inserted in pFA6a-kanMX6 plasmid [60] after the kanMX6 cassette using Gibson assembly cloning (NEB, E2611). FRB tag [23] was then inserted after the start codon by using Gibson assembly cloning and used as template for PCR ...
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bioRxiv - Microbiology 2022Quote: ... the corresponding ORFs were amplified from the WT strain 536 and cloned into the pUC18 plasmid (ampicillin 100 μg/mL) using BamHI and SphI enzymes (New England Biolabs, USA) (ECP_3022 ...
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bioRxiv - Biophysics 2022Quote: ... Both insert and vector were digested using the said restriction enzymes and ligated to form a circular plasmid using T4 DNA ligase (NEB, #M0202L). Sequence was verified by sanger sequencing.
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bioRxiv - Cell Biology 2022Quote: All plasmids reported in this work were constructed using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs Cat. #E2621), based on the Gibson assembly method (Gibson et al. ...
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bioRxiv - Cell Biology 2022Quote: ... the PCR product and the pDRF1-GW plasmid were digested using BamHI-HF and NheI-HF (New England Biolabs, Ipswich, MA) and the PCR product was ligated into the plasmid using T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly® Cloning Kit from New England Biolabs)57,58 or via QuickChange (Q5® Site-Directed Mutagenesis Kit from New England Biolabs).59 Recombinant expression of motors in E ...
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bioRxiv - Immunology 2022Quote: Point mutations were introduced through linearizing the plasmid by PCR with primers containing the intended mutations (Supp. Table S1) followed by reverting to circular plasmid with Gibson Assembly Master Mix (M5510A, NEB, USA).
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bioRxiv - Microbiology 2023Quote: An integration cassette containing two regions of homology flanking a kanamycin-resistance gene was engineered on a pUC backbone plasmid encoding ampicillin resistance using the NEBuilder HiFi DNA Assembly mastermix (NEB E2621L). A counter-selectable marker based on PheS77 was engineered under the constitutive SpeI promoter and cloned into the pUC plasmid (generating pUC-BC ...
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bioRxiv - Molecular Biology 2022Quote: RLuc-P2A-X-P2A-Fluc plasmids (where X represents a variable sequence) were assembled using NEB Builder HiFi DNA Assembly Cloning Kit (New England Biolabs, #E2621S) by combining the original plasmid digested with HindIII (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... were generated by deletion of the unwanted DNA from the parental plasmid using the Q5 Site-Directed Mutagenesis Kit (NEB BioLabs). The R763G point mutation was introduced into D1-A1 by PCR mutagenesis ...