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Citations for New England Biolabs :
1951 - 2000 of 2293 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: Approximately 6ng of each loxcode cassette was combined with approximately 75ng of the linear digested plasmid pool in a final 10uL of 1x HiFi assembly reaction (NEB). This was incubated on a thermocycler at 50C for 15min ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... 2018).Plasmid and inserts were digested with KpnI and BglII or SacII restriction enzymes (New England Biolabs, Ipswich, MA, USA) following manufacturer instructions ...
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bioRxiv - Pathology 2024Quote: ... Prior to transformation the bait plasmids were linearised in the URA3 gene by PCR or restriction digest (BbsI/Esp3I/BstBI (NEB)) to allow integration into the genome ...
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bioRxiv - Microbiology 2024Quote: ... 25 ng of linearised plasmids were ligated with 75 ng of purified VH and VL by Gibson Assembly (New England BioLabs). The reaction was performed in a final volume of 5 μL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The construct pcDNA4TO-TwinStrep (TS)-SNAP-β2AR was generated by amplification of the SNAP and β2AR sequences of the pSNAPf-ADRB2 plasmid (NEB) and inserted into pcDNA4TO-TS using Gibson assembly (Heydenreich et al. ...
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bioRxiv - Biophysics 2023Quote: ... A vector backbone was generated using pHR-dSV lentiviral plasmid digested with Mlu1 and Not1 restriction enzymes (New England Biolabs). Construction of the pHR-dSV-TMEM-PhoCl-IRAK4-mScarlet fusion was achieved by Gibson Assembly of the three components.
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bioRxiv - Neuroscience 2024Quote: ... DIG- and FITC-labeled antisense RNA probes were generated from 1 µg of linearized plasmid template using T7 or Sp6 RNA polymerases (NEB) and DIG-11-UTP (Roche) ...
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bioRxiv - Microbiology 2024Quote: Plasmid pET28bR109H for overexpression of the R109H mutant was obtained by site-directed mutagenesis of plasmid pET28bRho (kindly provided by Pr. James Berger, Johns Hopkins University) using the NEBuilder HiFi kit (New England Biolabs). The R109H mutant was overexpressed in BL21(DE3)pLysS cells carrying the pET28bR109H plasmid and purified as described for WT Rho 105.
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bioRxiv - Genomics 2024Quote: ... 1 μg of each sublibrary plasmid was digested at 37°C for 5 hours with NheI-HF and SacI-HF (New England Biolabs), incubated with rSAP for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... were generated from a pENTR cyfip2-EGFP plasmid [32] using custom primers and the Q5 Site Directed Mutagenesis Kit (NEB) to induce the desired C179R (ΔRac1 ...
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bioRxiv - Neuroscience 2024Quote: ... The codon-optimized APEX2-V5 sequence was synthesized as a gBlock (Integrated DNA Technologies) and inserted into the TOPO genomic sequence plasmid using the NEBuilder HiFi DNA assembly master mix (New England Biolabs). CRISPR guide RNA (gRNA ...
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bioRxiv - Neuroscience 2024Quote: ... a V5 tag was inserted after the start codon of Ten-m cDNA (isoform B) in the plasmid pUAST-attB-Ten-m5 using the Q5 site-directed mutagenesis kit (New England Biolabs). To generate the UAS-V5-Ten-m-ΔICD and UAS-V5-Ten-m-ΔECD constructs ...
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bioRxiv - Genetics 2023Quote: ... and incubated with restriction enzyme-digested CD510-B1 plasmid (SystemBio) and assembled with Gibson Assembly Master Mix (New England Biolabs).
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bioRxiv - Biochemistry 2024Quote: ... were PCR amplified with indicated primers (see Table S1) and inserted into backbone plasmids via restriction digest and ligation (all enzymes from NEB) or Gateway cloning (Thermo) ...
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bioRxiv - Cell Biology 2024Quote: ... The amplicons were digested with SacI and EagI ligated into the plasmid containing the ATG8 promoter linearized with HindIII (New England Biolabs) and SalI (New England Biolabs) ...
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bioRxiv - Bioengineering 2024Quote: ... Digested backbone plasmid and annealed spacer sequences were assembled using NEB High-Fidelity DNA Assembly 2x Master Mix (New England Biolabs) in a reaction containing 5 µl of the master mix 2 µl of the digested backbone and 3 µl of the PCR annealed spacer ...
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bioRxiv - Bioengineering 2024Quote: ... And cloned into a expression plasmid under the control of the EF1α using NEB High-Fidelity DNA Assembly 2x Master Mix (New England Biolabs). To generate 3’ and 5’ stop codon reporters ...
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bioRxiv - Microbiology 2024Quote: ... the plasmid pME6000 was digested by HindIII and the PCR-amplified gene of interest was inserted by Gibson cloning (NEB). For this ...
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bioRxiv - Cell Biology 2022Quote: Mouse phogrin was obtained from the IMAGE consortium (clone BC_133678) and CLIPf from NEB. The fusion protein was generated by standard molecular cloning techniques ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse Adnp was amplified from pENTR223.1-Adnp using Q5 High Fidelity DNA Polymerase (NEB) and primers containing 6xHis tag to insert it into the C-terminal region of Adnp ...
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bioRxiv - Microbiology 2021Quote: ... reporter genes (obtained as gBlock Gene Fragments from IDT) were then ligated into the linearized plasmid by Gibson Assembly (NEBuilder® HiFi DNA assembly, NEB). The phosphoprotein gene start and the hemagglutinin (H ...
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bioRxiv - Evolutionary Biology 2021Quote: ... a donor DNA containing two homology arms flanking the DsRed sequence for homology repair were cloned into the pHD-ScarlessDsRed plasmid using Q5® High-Fidelity DNA Polymerase (New England Biolabs). Left homology arm contained the sequence in 3R:16188287-16189463 ...
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bioRxiv - Molecular Biology 2019Quote: ... of pA-Dam protein was incubated with 500 ng of unmethylated plasmid in 20 µL of 1x dam MethylTransferase buffer (New England BioLabs #M0222S) supplemented with 80 µM S-adenosylmethionine (SAM ...
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bioRxiv - Microbiology 2021Quote: E248R deletion mutant proteins of distinct domains (Δ constructs) were generated from E248R WT plasmid by site direct mutagenesis using the Q5 mutagenesis kit (New England Biolabs) as follows ...
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bioRxiv - Genetics 2021Quote: All constructs used for homologous recombination into the chromosome were constructed in a pUC19 plasmid vector (New England Biolabs, Ipswich, MA). PCR was used to amplify DNA fragments and Gibson assembly cloning (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplified PCR products were ligated into the recombinant plasmid pZE0-P-MglE-T using the recommended standard USER cloning protocol (NEB, UK).
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bioRxiv - Molecular Biology 2020Quote: ... and the next two fragments were inserted into resulting plasmid using NEBuilder®HiFi DNA Assembly Cloning Kit (New England Biolabs). Fragments were amplified with primers (Table S1 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The mcr-1 gene along with its natural promoter was PCR-amplified from the natural PN16 (IncI2) plasmid using Q5® High-Fidelity DNA Polymerase (New England BioLabs). The amplified and purified mcr-1 fragment was assembled together with PCR-amplified pSEVA121 backbone using NEBuilder® HiFi DNA Assembly Master Mix according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The resulting fragment is then ligated into a digested pCDNA3-YFP Basic plasmid that had been cut with Kpn1 and Xho1 using a standard T4 DNA Ligase protocol (NEB #M0202). Once the insert ...
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bioRxiv - Molecular Biology 2021Quote: TCF20(1-1268)-mEGFP construct was generated from the TCF20-mEGFP plasmid using the Q5 Side-Directed Mutagenesis Kit (NEB, E0554S) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: All single gRNA-expressing plasmids were assembled by Gibson assembly reaction using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). Depending of the plasmid features ...
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bioRxiv - Genetics 2019Quote: ... and integrated with hygromycin resistance cassette amplified from plasmid pUMa1507 (Terfrüchte et al. 2014) by Gibson Assembly (New England Biolabs, Ipswich, USA).
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bioRxiv - Cell Biology 2019Quote: ... red fluorescent protein coding sequence (dsRed) were cloned into the above derivative tetracycline inducible plasmid with NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs) at NheI and MluI restriction sites (pLVD-GRASLND and pLVD-dsRed) ...
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bioRxiv - Neuroscience 2019Quote: ... and the coding sequence of TurboRFP fused to the 3’ UTR of the ribosomal gene Ppa-rpl-23 (Schlager et al., 2009) were cloned into the pUC19 plasmid using Golden Gate Assembly Mix (New England BioLabs, E1600S) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: IVT was performed using the T7 promoter of the pcDNA3.3_NDG plasmid and HiScribe T7 ARCA mRNA kit with tailing (NEB #E2060S, Ipswich, MA). Whole kit was used with 20 ug DNA following manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Donor sequences which typically contain 500-bp upstream and 500-bp downstream of the editing sites were amplified by PCR and ligated into the linearized targeting plasmid (digested by XhoI (NEB, USA)) using the ClonExpress One Step Cloning Kit (Vazyme ...
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bioRxiv - Genetics 2019Quote: ... as shown in figure S2) were introduced into the ARSA plasmid by using Site-Directed Mutagenesis Kit (Cat. No. E0552s, NEB, USA). The sequences of mutated cDNA vectors were confirmed using the ABI3500 sequencer (Applied Biosystems Inc. ...
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bioRxiv - Microbiology 2021Quote: ... the SAG1 3’UTR was amplified from pNJ-26 and cloned into the tagging plasmid to replace DHFR 3’UTR by Gibson assembly (NEB, E5520S). BAG1-mCherry GCaMP6f reporter tachyzoites were co-transfected with 10 μg of pSAG1::CAS9-U6::sgDHFR 3’UTR and 2 μg of PCR amplified P2A-mTagBFP2-HXGPRT flanked with 40 bp homology regions ...
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bioRxiv - Microbiology 2021Quote: ... Point mutations to the binK sequence on the plasmid were designed using the NEBaseChanger tool and constructed with the Q5 site-directed mutagenesis kit (New England BioLabs, Inc.). The constructed plasmid was transformed into either electrocompetent or chemically competent DH5α λpir E ...
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bioRxiv - Bioengineering 2021Quote: ... homology arms were amplified from genomic DNA and introduced to the MCS of the linearized SIBR-Cas plasmid using the NEBuilder® HiFi DNA Assembly Master Mix (NEB). The plasmid was linearized using Esp3I (NEB) ...
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bioRxiv - Bioengineering 2020Quote: ... 30 bp at the 3’ end of PAM site was selected as the guide sequence and cloned into pCASCADE plasmid using Q5 site-directed mutagenesis (NEB, MA) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The G288D mutation was then generated from this pET15b-AcrB-6xHis plasmid using the Q5® site-directed mutagenesis kit (New England Biolabs). All constructs were verified by DNA sequencing (Eurofins MWG).
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bioRxiv - Biochemistry 2020Quote: ... The G288D mutation was generated from this pBR-AcrBWT plasmid using the Q5® site-directed mutagenesis kit (New England Biolabs). All constructs were verified by primer walking DNA sequencing (Eurofins MWG).
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bioRxiv - Biochemistry 2020Quote: ... 1.5 mg of pNL003 plasmid (38) was linearized by incubation at 37 °C for 2-4 h in the presence of EcoRV (NEB R0195S). Transcription reactions (10 mL ...
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bioRxiv - Neuroscience 2020Quote: ... PCR was performed with 10 ng of plasmid DNA template using Q5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs) in a final reaction volume of 25 μL according to the manufacturer’s recommendations except that the extension time was set to 80 seconds per kb for a total of 20 cycles ...
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bioRxiv - Neuroscience 2021Quote: ... Point variants were introduced into hFibcd1 cDNA while cloned in the intermediate pDONR201 plasmid using the Q5 site-directed mutagenesis kit (NEB #E0554), following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... The plasmid of pAC-BETAipi-ispA/dxs was constructed by using Gibson assembly kit (Gibson Assembly® Master Mix, New England Biolabs). The insert was amplified from genomic DNA of E ...
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bioRxiv - Microbiology 2020Quote: Plasmid construction was performed by: PCR amplification of the fragment to insert in the plasmid with Q5® High-Fidelity DNA Polymerase (New England Biolabs), digestion with the appropriate FastDigest restriction enzyme (ThermoFisher) ...
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bioRxiv - Neuroscience 2020Quote: ... 3 DNA fragments were generated with PCR from genomic DNA or plasmids using Q5 High-Fidelity DNA Polymerase (NEB Cat# M0491). Fragment 1 included the genomic DNA between guide 1 and the KI site plus flipped guide 2 (with PAM ...
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bioRxiv - Microbiology 2021Quote: ... All generated plasmids of this study (Table 4) were cloned with restriction endonucleases and T4 ligase from NEB (New England Biolabs) or Gibson assembly (NEBuilder® HiFi DNA Assembly ...