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Citations for New England Biolabs :
1601 - 1650 of 7434 citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The resulting mRNA is used for library construction for RNA-seq using NEBNext mRNA Library Prep Reagent set for Illumina (NEB, USA). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... MUT) by using a different set of type IIS enzymes (BbvI, New England Biolabs #R0173, and BfuAI, New England Biolabs #R0701). Importantly ...
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bioRxiv - Plant Biology 2022Quote: ... The small RNA libraries were constructed using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, E7300S) kit and sequenced on the Illumina HiSeq 2500 platform at Berry Genomics (Beijing ...
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bioRxiv - Microbiology 2022Quote: ... LAMP reactions were set up using the NEB WarmStart® LAMP Kit (DNA & RNA) (E1700S, New England Biolabs, Ipswich, Massachusetts, USA) and using the two designed primer sets ...
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bioRxiv - Neuroscience 2022Quote: ... sequencing libraries were constructed using the NEBNext ChIP-seq Library Prep Reagent Set for Illumina and a NextSeq 500 Illumina sequencer (New England BioLabs Inc.).
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bioRxiv - Microbiology 2022Quote: ... Illumina DNA library preparation was performed using NEBNext® DNA Library Prep Master Mix Set for Illumina® (New England Biolabs), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Libraries of small RNA were constructed using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England BioLabs, #E7330) following manufacturer’s recommendations ...
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bioRxiv - Genomics 2024Quote: ... IVT reactions were set up in 30 μl total volume with the HiScribe T7 High Yield RNA Synthesis Kit (NEB E2040S) with 2 μl of each NTP ...
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bioRxiv - Bioengineering 2023Quote: ... The amplification of repetitive units was generated by BioBrick restriction enzyme assembly using NheI and SpeI enzyme sets (New England BioLabs, USA). Subsequently ...
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bioRxiv - Bioengineering 2023Quote: ... DECIPHER comes with a set of 214 restriction enzymes for sale at New England Biolabs (referred to as NEB restriction enzymes). To obtain the null distribution of CoV restriction maps ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed from 800 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, New England Biolabs, NEB-E7560S) and the small RNA fraction was sequenced on the NextSeq 500 System (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were built from 250 ng of total RNA using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® following manufacturer’s recommendations (New England Biolabs). Thirteen PCR cycles were completed to finalize the library preparation ...
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bioRxiv - Microbiology 2023Quote: ... custom adapters (Table S1) were ligated using a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs, E6040S), and the transposon-genome junction was amplified with primers (Table S1 ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Small RNA libraries were then prepared from 10 ng of small RNA using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs #E7300S) according to the manufacturer’s recommendation using NEBNext Multiplex Oligos for Illumina (Index Primer Set 1 ...
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bioRxiv - Plant Biology 2023Quote: ... and small RNAs of 15-40 nt were excised from the gel and used to construct a library using “NEB Next Multiplex Small RNA Library Prep Set” for Illumina (New England Biolabs, E7300). High-throughput sequencing using the Illumina NextSeq500 platform was performed at BerryGenomics ...
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bioRxiv - Molecular Biology 2023Quote: ... cloning and data analysis were performed as previously described [38] except that the RPF libraries were prepared using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB E7300). The trimmed RPF reads were mapped to C ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed from 300 ng (liver) or 200 ng (hypothalamus) total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, New England Biolabs, NEB-E7560S) and the small RNA fraction was sequenced on the NextSeq 500 System (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Double-stranded blunt-end libraries were constructed from the extracted DNA using NEBNext DNA Prep Master Mix Set E6070 (New England Biolabs Inc.) and sequenced (80bp and 100bp single read ...
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bioRxiv - Cell Biology 2023Quote: ... One hundred nanograms of total RNA was used to prepare sequencing libraries using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB #E7560S). The 3’ adapter was ligated and the reverse transcription primer hybridized ...
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bioRxiv - Cell Biology 2024Quote: ... The entire yield was used to generate sequencing libraries following manufacturer’s protocol for NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Cat #E7300S), using 12 PCR cycles ...
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bioRxiv - Genomics 2021Quote: ... 0.1μL of 100μM Smart-seq2 Oligo-dT primer (SigmaAldrich) and 1μL dNTP mix (10mM each, NEB). In P1 ...
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bioRxiv - Microbiology 2021Quote: Two-hundred nanograms of total RNAs were reverse-transcribed using Random primer 6 (New England Biolabs) and Superscript III reverse transcriptase ...
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bioRxiv - Cancer Biology 2021Quote: ... Poly(A)-cDNA was amplified using AL1 primer (ATTGGATCCAGGCCGCTCTGGACAAAATATGAATTCTTTTTTTTTTTTTTTTTTTTTTTT) and a blend of Taq polymerase (NEB) and Phusion (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... castellii genomic sequence in between the ORFs NCAS0D00680 and NCAS0D00690 was amplified (primers: Ncas_Int618_For 5′-GTTCGCCGGCCTTCCCGCGCTATGAAATTA and Ncas_Int618_Rev 5′-ATCAGGCGCCGAGCATAACCGCTCAAATGC) and inserted between the NaeI and KasI (NEB) restriction sites ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and 0.75 U of Taq (0.325 U for primers 31, 32 and 41; New England Biolabs). Primer concentrations of 200 nm of each primer were added to reactions involving loci A3Sym_01 ...
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bioRxiv - Molecular Biology 2019Quote: ... We used the NEBNext Multiplex Oligos for Illumina (Index Primers #1 - #24) (NEB, E7335S and E7500S) as the unique index primers ...
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bioRxiv - Systems Biology 2019Quote: ... one-third of the DNA sample was amplified using Illumina indexing primers (NEB Catalog no. 7600L). PCR product was size selected by running on 1.5% agarose gel ...
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bioRxiv - Microbiology 2020Quote: ... 1µL Random Primer Mix (ProtoScript II First Strand cDNA Synthesis Kit, New England Biolabs, https://www.neb.ca) was added to 7 µL extracted RNA and denatured on SimpliAmp thermal cycler (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... 600 ng of total RNA were mixed with 0.83 μL 100 mM tris pH 7.5 and 0.17 μL 3 mg·mL−1 Random Primers (NEB) to a volume of 5.25 μL ...
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bioRxiv - Microbiology 2019Quote: ... adjacent primers containing the mutation of interest were 5′-phosphorylated using T4 polynucleotide kinase (NEB, M0201S) using the buffer from the T4 DNA ligase ...
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bioRxiv - Microbiology 2019Quote: ... 500nM gene specific or 65nM 16S rRNA primer and 0.5 units Hot Start Taq Polymerase (NEB), and 10ng of template cDNA ...
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bioRxiv - Cancer Biology 2019Quote: ... extracting domain D2 from pDONR221/CD4 using primer pair P4 and performing a Gibson assembly (NEB) with the PCR products ...
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bioRxiv - Biochemistry 2020Quote: ... vectors using Gibson Assembly (see Table S2 for primers) and NEB stable Competent E.coli (NEB #C3040). For plasmid transfection ...
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bioRxiv - Plant Biology 2020Quote: ... Samples were amplified with the appropriate barcoded primers using Phusion high-fidelity polymerase (New England Biolabs). The PCR recipe and thermocycler program can be found in Table S2.
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bioRxiv - Systems Biology 2021Quote: ... Polylinker libraries were amplified using primers BC_CRX_Nested_F and BC_CRX_R (Supplementary file 6) for 30 cycles (NEB Q5) at an annealing temperature of 67C and then purified with the Monarch PCR kit ...
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bioRxiv - Genomics 2021Quote: ... Purified cDNA was indexed with i5 and i7 primers (catalog #7600S, New England BioLabs, Tokyo, Japan), and then purified and size selected for a range of 400 to 2000 bp using 11% and 11.5% PEG and DynaBeads [19].
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bioRxiv - Microbiology 2021Quote: ... 500nM gene specific or 65nM 16S rRNA primer and 0.5 units Hot Start Taq Polymerase (NEB), and 10ng of template cDNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... with Gibson Assembly primers compatible with the NEBuilder HiFi DNA Assembly Kit (New England BioLabs; E2621). β-arrestin 2 was subsequently assembled into pBiT1.1-C[TK-LgBiT] ...
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bioRxiv - Neuroscience 2021Quote: ... When paired with the end primers two amplicons were created (Phusion High-Fidelity DNA Polymerase; NEB) that overlap with each other and the digested vector ends ...
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bioRxiv - Cancer Biology 2020Quote: ... 6.25μl forward and barcoded reverse primer (10μM, IDT) and 25μl Phusion High-Fidelity master mix (NEB). The PCR program was ...
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bioRxiv - Cancer Biology 2020Quote: ... Illumina compatible NEBNext Multiplex Dual Index Primer pairs and Adaptors (New England Biolabs, Cat. No. E6440S) were then ligated to the ends of the size-selected chimeric fragments ...
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bioRxiv - Biophysics 2021Quote: ... Primers were designed for the replacement of each of the 4 loops using Q5 polymerase (NEB) PCR reaction and a KLD enzyme mix (NEB) ...
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bioRxiv - Biophysics 2020Quote: ... The primers above were designed with overlaps for Gibson Assembly according to manufacturer’ s specifications (NEB). The three PCR generated fragments were assembled in the presence of pUC18 digested with NdeI and PstI (NEB ...
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bioRxiv - Cell Biology 2021Quote: The ~1kbp PFA66 targeting region was amplified using the primers PFA_NotI_F and PFA_MluI_R and cloned into pSLITGD using NotI-HF and MluI (NEB)17 (kind gift of Tobias Spielmann) ...
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bioRxiv - Molecular Biology 2019Quote: ... castellii genomic sequence downstream of the ORF NCAS0C00690 was amplified (primers: Ncas_Int696_For: 5′-GGCCGGTACCAATTCATCTAGCAGGATGTAAAATG; Ncas_Int696_Rev: 5′-GAAAGCCGGCGTAGAGCATGCGAGGTTTGG) and inserted between the KpnI and NaeI (NEB) restriction sites in pRS404 (3) ...
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bioRxiv - Molecular Biology 2019Quote: ... castellii genomic sequence upstream of the ORF NCAS0E03540 was amplified (primers: Ncas_Int701_For 5′-ATTCGGATCCTGCAGGCTGTTTGCTGTACT; Ncas_Int701_Rev 5′-GGTGGCGGCCGCGGGGTAACTATCCGCGTCTAA) and inserted between the BamHI and NotI (NEB) restriction sites in pRS402 (5) ...
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bioRxiv - Immunology 2021Quote: ... 2 µL of random primer mix 60 µM (cat. n° S1330S, NEB, New England Biolabs, USA) and 4.8 µL of nuclease-free water ...
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bioRxiv - Immunology 2021Quote: ... 2 µL of random primer mix 60 µM (cat. n° S1330S, NEB, New England Biolabs, USA) and 4.8 µL of nuclease-free water ...
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bioRxiv - Neuroscience 2020Quote: ... followed by second strand synthesis and subsequent removal of excess primers using Exonuclease I (NEB, M0293). cDNA samples were pooled per sets of 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... and indexed common primers (Vazyme, Cat.No.TD202) in 1 x Q5 High-Fidelity Master Mix (NEB, Cat.No.M0492). Then index PCR was performed as follow ...