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Citations for New England Biolabs :
1801 - 1850 of 7434 citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Filtered (0.45 µm) supernatants were treated with 1 U/ml DNAse I (NEB; 1 h, RT) and purified through a 20% sucrose cushion (2 h ...
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bioRxiv - Genomics 2023Quote: ... cDNA was synthesised from 1ug of DNase treated RNA using the LunaScript® RT SuperMix (NEB), according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... total RNA (a minimum of 175 ng) was reverse transcribed using LunaScript RT (New England Biolabs) followed by dilution of 1:5 or 1:10 depending on the normalized RNA input concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were prepared using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Quantitative RT-PCR was performed with an Mx3000P qPCR system (Agilent ...
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bioRxiv - Physiology 2023Quote: ... Samples were reversed transcribed using the LunaScript RT SuperMix Kit (New England BioLabs, Ipswich, Massachusetts, USA) and relative gene expression was quantified via CFX-96 Real-Time PCR Detection System (RRID:SCR_018064 ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR was performed in technical triplicates using the Luna® Universal qPCR Master Mix (NEB) in a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA synthesis was performed using the Luna script RT Supermix kit (New England Biolabs, Cat#M3010L). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Synthetic Biology 2024Quote: ... The first cDNA synthesis was initiated by adding 1 U/µL RT at final concentration (NEB #M0277L for AMV RT ...
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bioRxiv - Synthetic Biology 2024Quote: ... A NEB Monarch® Plasmid Miniprep Kit was used for RT-DNA extraction (New England Biolabs). Following miniprep ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of total RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instructions and 1 µl cDNA was used as a template for a 10 µl qPCR reaction ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesised according to the protocols of the LunaScript RT Supermix Kit (New England Biolabs) and for qPCR amplification ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 µM of each primer and 1U of Phusion® polymerase (New England Biolabs® Inc., U.S.A). The subsequent thermal profile was as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... Tn5 treated DNA was amplified with Nextera Library primers using NEBNext library amplification master mix (NEB M0541) and sequenced on an Illumina NextSeq ...
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bioRxiv - Genomics 2020Quote: ... 2.5 μl of 10 μM NEBNext i5 and 2.5 μl of 10 μM NEBNext i7 primers (NEB) in a final volume of 50 μl ...
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bioRxiv - Bioengineering 2022Quote: ... these products were further amplified using NEBNext Dual Index Primers for Illumina sequencing (New England Biolabs, E7600) for ten cycles ...
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bioRxiv - Evolutionary Biology 2019Quote: RNA was extracted and reverse transcribed using random primers and M-MuLV reverse transcriptase (New England Biolabs) and a PCR product of about 300 nucleotides from a coding sequence was than amplified by PCR using Q5® High-Fidelity DNA polymerase (New England Biolabs) ...
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bioRxiv - Microbiology 2019Quote: ... verticillioides with the primers FvGbb2-GFP-F/R using Q5® High-Fidelity DNA Polymerase (NEB: M0491S). The DNA fragment was introduced to the pKNTG plasmid KpnI and HindIII sites via In-Fusion-HD cloning kit (Takara Bio USA ...
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bioRxiv - Cell Biology 2019Quote: ... 0.25μl of each primer (from 25μM stock) and 4.5μl of Luna Universal Master Mix (New England Biolabs). The q-RT-PCR reactions were run on the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... from our construct using primers 3xFLAG_F and 3xFLAG_R and cloned into petSUMO_NSP1_WT vector using Gibson Assembly® Master Mix (NEB). The resulting proteins were expressed in Escherichia coli BL21(DE3 ...
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bioRxiv - Immunology 2021Quote: ... 240 nM dT-primer* (Metabion, Planegg, Germany) and 4 U RNase Inhibitor (New England Biolabs, Frankfurt, Germany). Reverse transcription and addition of the template switch oligo was performed at 42 °C for 90 min after filling up to 10 μl with RT buffer mix for a final concentration of 1x superscript II buffer (Invitrogen) ...
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bioRxiv - Bioengineering 2020Quote: ... these products were further amplified using NEBNext Dual Index Primers for Illumina sequencing (New England Biolabs, E7600), after 10 cycles of 98°C for 10 s ...
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bioRxiv - Bioengineering 2020Quote: ... these products were further amplified using NEBNext Dual Index Primers for Illumina sequencing (New England Biolabs, E7600), after 10 cycles of 98°C for 10 s ...
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bioRxiv - Microbiology 2021Quote: ... was used for library construction with NEBNext Multiplex Oligos for Illumina Dual Index Primers (New England Biolabs). Following equimolar pooling ...
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bioRxiv - Molecular Biology 2022Quote: ... the modifications included employment of deoxy uridine containing primers and USER enzyme mix (New England Biolabs, UK) to create sticky ends that would facilitate the circularization of plasmid [9] ...
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bioRxiv - Microbiology 2022Quote: ... as follows: 1µL of 2µM MBTUni-12 primer (5’-ACGCGTGATCAGCRAAAGCAGG-3’) + 1µL 10mM dNTPs Mix (NEB #N0447S) + 8µL Nuclease-free water (Ambion ...
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bioRxiv - Biophysics 2022Quote: ... The labeled primer was purified by G-25 microspin column (Cytvia) and Monarch DNA purification kit (NEB). The successful conjugation of three digoxigenin moieties was confirmed by the shift of the molecular weight using denaturing urea polyacrylamide gel electrophoresis ...
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bioRxiv - Plant Biology 2022Quote: ... Inserts were amplified with gene specific primers using Q5 High Fidelity DNA polymerase (M0491, New England Biolabs), followed by purification of amplicons using the Nucleospin Gel and PCR Clean-up (Machery-Nagel ...
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bioRxiv - Cancer Biology 2021Quote: ... Poly(A)-cDNA was amplified using an AL1 primer (ATTGGATCCAGGCCGCTCTGGACAAAATATGAATTCTTTTTTTTTTTTTTTTTTTTTTTT) and a blend of Taq polymerase (NEB) and Phusion (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... to digest the bottom strand of dsDNA and truncated the 5’ primer region with USER enzyme (NEB). We added 5 M NaCl 20% v/v to the oligo mix and pre-annealed by heating it at 95°C for 3 minutes ...
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bioRxiv - Biophysics 2022Quote: ... a third primer ACTB K118TAG phosphorylated at its 5’ end and Taq DNA ligase (New England Biolabs) were added to the reaction mixture ...
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bioRxiv - Genomics 2020Quote: ... The double stranded cDNAs were amplified using Illumina adapter-specific primers and LongAmp Taq DNA polymerase (NEB). After 16 cycles of PCR (8?minutes for elongation time) ...
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bioRxiv - Molecular Biology 2019Quote: ... primer annealing at 23°C for 30 s) template DNA was mixed with T7 RNA polymerase(NEB), 50 mM MgCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse primer Y111E: 5’-TTGATGGAGACATTCTTC-3’) using the Q5 site-directed mutagenesis kit (E0554S, New England Biolabs) to generate a point mutant ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10 μM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1 μL 10 μM NEBNext Universal PCR Primer in 20 μL total volume ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10 μM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1 μL 10 μM NEBNext Universal PCR Primer in 20 μL total volume ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10 μM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1 μL 10 μM NEBNext Universal PCR Primer in 20 μL total volume ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10 μM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1 μL 10 μM NEBNext Universal PCR Primer in 20 μL total volume ...
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bioRxiv - Genomics 2021Quote: ... We amplified bisulfite-converted libraries with NEB next indexing primers (New England Biolabs Inc. cat. No. E7335S).
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bioRxiv - Immunology 2021Quote: ... Libraries were indexed using NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (NEB #644OS/L). Library prep generated cDNA was quantified and analyzed using Agilent DNA chip and Qubit DNA dye ...
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bioRxiv - Genomics 2021Quote: ... cDNA was pooled and cleaned using SPRI beads and excess primers digested by incubation with ExonucleaseI (NEB; 30 min @ 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2 μM reverse primer and 5 μL Luna® Universal Probe qPCR Master Mix (New England Biolabs)] amplified using the Prism7900HT sequence detection system (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... the above construct was amplified using primers 17 and 18 and integrated into BamHI/PacI-digested (NEB) pUPRT plasmid by Gibson assembly ...
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bioRxiv - Microbiology 2022Quote: ... Post-RCA DNA was amplified using these primers with a Q5 Hot Start polymerase (New England Biolabs). The product was run on a 2% gel to confirm specific binding before sending the PCR product to GeneWiz for Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... Primer pairs M01/M02 and M03/M04 were used with the Q5 site-directed mutagenesis kit (NEB) to introduce point mutations for the T161V and D296N substitutions ...
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bioRxiv - Microbiology 2024Quote: ... and 750 bp upstream and 747 bp downstream were cloned using primers JCP343/344 into SmaI (NEB)-digested pTOX5 allelic exchange vector44 ...
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bioRxiv - Microbiology 2024Quote: Suitable primer pairs (Table S5) were designed utilizing the Q5 site-directed mutagenesis kit (New England Biolabs) to generate mutated amidases ...
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bioRxiv - Cell Biology 2024Quote: ... using NEBNext Multiplex Oligos for Illumina – 96 Unique Dual Index Primer Pairs (#6440S, New England Biolabs, US). All generated cDNA libraries were amplified with 7 cycles of final PCR.
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bioRxiv - Genomics 2023Quote: ... with NEBNext Multiplex Oligos (96 Unique Dual Index Primer Pairs) for Illumina Barcodes (New England Biolabs, USA) and sequenced on an Illumina NovaSeq 6000 S2 150bp PE flow cell at the Duke Center for Genomic and Computational Biology Sequencing and Genomic Technologies Core.
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bioRxiv - Cancer Biology 2023Quote: ... SYBR™ green and 0.5 µM of sample-specific P5/P7 barcoded primer mix (New England Biolabs). Bead clean-up and purification was performed using SPRI beads (Beckman Coulter) ...
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bioRxiv - Neuroscience 2023Quote: ... and amplified with primers containing indexes for sequencing using Q5 High-Fidelity 2xMaster mix (New England Biolabs).