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Citations for New England Biolabs :
1501 - 1550 of 7434 citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Sequencing libraries were prepared with the NEBNext mRNA library Prep Master Mix Set for Illumina (New England BioLabs, cat#: E6110), passed Qubit bioanalyzer and qPCR QC analyses ...
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bioRxiv - Molecular Biology 2023Quote: ChIP libraries were prepared for Illumina NextSeq 500 using NEBNext ChIP-Seq DNA Sample Prep Master Mix Set for Illumina (E6240; NEB) and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 ...
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bioRxiv - Microbiology 2024Quote: ... Bisulfite-converted RNAs were then end-repaired and subjected to NEBNExt Small RNA Library Prep Set for Illumina (NEB, E7330L), according to manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Double-stranded DNA was converted into an Illumina sequencing library using an NEBNext Quick DNA Library Prep Master Mix Set for 454 (New England BioLabs) with 20 μl of DNA extract without DNA fragmentation ...
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bioRxiv - Molecular Biology 2024Quote: ... or 2μg (dual-guide) per well were set up using the Q5 Hot Start High-Fidelity 2× Master Mix (NEB #M0494) in a total volume of 50μl ...
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bioRxiv - Genetics 2019Quote: ... using Gibson assembly PCR (NEB) to create pEAE393 (Mlh1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and PCR amplified (NEB #M0531). Libraries were run on an Illumina Hi-Seq 4000 sequencer ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were generated by PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB). The resulting libraries were multiplexed and sequenced in a HiSeq 4000 pair-end lane producing 100M of 49-bp pair end reads per sample.
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bioRxiv - Molecular Biology 2021Quote: ... Single step PCR was performed using a One-Taq™ PCR reaction kit (NEB, USA) with 30 amplification cycles and 2 ng of original circular-DNA extract as the DNA template ...
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bioRxiv - Microbiology 2019Quote: ... Amplicon PCR was performed using the Phusion High-Fidelity PCR Master Mix (New England Biolabs) and previously described barcoded primers37 to amplify the V1-V3 region of bacterial 16S rDNA ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products and digested plasmids were purified with the Monarch PCR & DNA Cleanup Kit (NEB). Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN).
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bioRxiv - Cancer Biology 2019Quote: ... All PCRs were performed with Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB).
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bioRxiv - Plant Biology 2021Quote: ... PCR was performed using High-Fidelity Phusion PCR Master Mix (NEB, Frankfurt am Main, Germany) (initial denaturation (98 °C ...
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bioRxiv - Genetics 2021Quote: ... PCR amplicons were purified using the Monarch PCR & DNA cleanup kit (New England BioLabs T1030) or the QIAQuick PCR purification kit (Qiagen 28104 ...
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bioRxiv - Cell Biology 2021Quote: ... The DNA segments were PCR amplified (Phusion High-Fidelity PCR Master Mix, NEB, UK, #M0531) and fused with Gibson recombination cloning method (Gibson Assembly Master Mix ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by 9 cycles of PCR enrichment using NEB High fidelity PCR kit (NEB, M0541S). The quality of ATAC libraries was assessed with Agilent Bioanalyzer with DNA High Sensitivity kit (Agilent Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR product was purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs, Inc.). The samples were run on a 1.5% Agarose (Merck ...
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bioRxiv - Bioengineering 2022Quote: ... PCR products were purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and then digested by BspHI and MfeI-HF (New England Biolabs) ...
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bioRxiv - Developmental Biology 2022Quote: ... The resulting PCR product was purified with the Monarch PCR and DNA Cleanup Kit (NEB) with elution in 20 microliters nuclease-free water ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were purified using the Monarch PCR and DNA cleanup kit (New England Biolabs), and electrophoresed on 1 % agarose gel to verify amplicon size.
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bioRxiv - Microbiology 2019Quote: ... The remaining PCR mixture was purified using the Monarch® PCR & DNA Cleanup Kit (NEB) and the DNA eluted with nuclease-free water ...
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bioRxiv - Developmental Biology 2019Quote: ... and PCR was amplified with high-fidelity 2X PCR Master Mix (New England Biolabs M0541). One-third of the maximum fluorescent intensity was used to determine the additional cycles ...
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bioRxiv - Genetics 2019Quote: ... and amplified by PCR using Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) to produce sub-pools for Gibson assembly (NEB) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The PCR products were purified with Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and subcloned using StrataClone PCR cloning Kit (Agilent Technologies ...
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bioRxiv - Synthetic Biology 2021Quote: ... 6 were generated by PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs #E0553) of an oligo that includes a T7 promoter ...
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bioRxiv - Genomics 2021Quote: ... Libraries were PCR-amplified using NEBNext High Fidelity 2X PCR MasterMix (New England BioLabs M0541) for 14 cycles ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified using a Monarch PCR & DNA Cleanup Kit (5 μg) from NEB and then visualized on an 0.8 to 1.2 % percent agarose gel resolved in Tris-acetate-EDTA electrophoresis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... PCR products were purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and submitted to the Massachusetts General Hospital DNA Core ...
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bioRxiv - Genomics 2022Quote: ... The PCRs were then purified with Monarch PCR & DNA Cleanup Kit (New England Biolabs, T1030) and Illumina adapters were added in another 2 rounds of PCR ...
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bioRxiv - Physiology 2024Quote: ... After the PCR 20ul of the PCR product were restriction digested with BamH1 (NEB #xxx) for 1 hr at 37C ...
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bioRxiv - Immunology 2024Quote: ... Purified round 2 PCR products were cloned using the NEB PCR Cloning Kit (NEB, #E1202) and sequenced with Sanger sequencing.
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bioRxiv - Developmental Biology 2023Quote: ... After samples were PCR-amplified using 1×NEBNext High-Fidelity PCR Master Mix (NEB, M0541S). Subsequent libraries were purified with the MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified using Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA). DNA concentration was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and PCR was amplified with high-fidelity 2x PCR Master Mix (New England Biolabs M0541). One-third of the maximum fluorescent intensity during a qPCR trial run was used to determine the additional cycles for library prep ...
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bioRxiv - Bioengineering 2022Quote: Genomic PCR was performed using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs) with the primers listed in Supplementary Table 1 ...
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bioRxiv - Systems Biology 2023Quote: ... PCR amplification was carried out in a reaction mixture containing PCR Master Mix (NEB, M0543S), 0.5 μM of each primer ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were generated by PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB). The resulting libraries were multiplexed and sequenced in a HiSeq 4000 paired-end lane ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR was performed with 2x NEBNext High-Fidelity PCR Master Mix (New England Biolabs, M0541S) and 10 μM primers (Table S2 ...
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bioRxiv - Genomics 2023Quote: ... The outward PCR experiments were performed using NEBNext High-Fidelity 2X PCR Master Mix (NEB). For a 50 µl reaction ...
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bioRxiv - Genomics 2023Quote: ... pooled oligonucleotides were amplified via PCR using NEBNext 2X Hi-Fi PCR Master Mix (NEB) and primers:
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were generated by PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB). The resulting libraries were purified using MinElute PCR Purification Kit (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: PCR and plasmid generation – All PCR for plasmid generation was conducted using Phusion polymerase (NEB) using primers listed in Supplemental Table 1 ...
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bioRxiv - Biochemistry 2023Quote: ... PCR purifications with the Monarch® PCR & DNA cleanup kit (New England Biolabs, Ipswich, USA), extraction of genomic DNA with the Monarch® Genomic DNA Purification Kit (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplicons were purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs).
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bioRxiv - Neuroscience 2023Quote: ... PCR amplified Domain 1 and PCR Domain 2 and HiFi DNA assmbley was performed (NEB). Transformation was performed in DH5α cells (REF) ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR product was purified using the Monarch PCR and DNA cleanup kit (T1030S, NEB) and dsRNA was synthesised using the T7 RiboMAX express system (P1700 ...
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bioRxiv - Bioengineering 2024Quote: ... PCR reactions were purified using the Monarch DNA PCR Cleanup Kit (New England Biolabs; T1030). For Gibson assembly of constructs ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were then PCR purified using a Monarch® PCR & DNA cleanup kit (NEB-T1030S) once a band of the correct size was visualized on a 1% Agarose gel ...
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bioRxiv - Synthetic Biology 2024Quote: ... Genomic DNA samples were PCR amplified with Q5 High-Fidelity 2X PCR Master Mix (NEB) based on the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... The pooled DNA was PCR-amplified using Phusion® High-Fidelity PCR Kit (NEB®), followed by purification with a Monarch® PCR & DNA Cleanup Kit (NEB®) ...