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Citations for New England Biolabs :
1851 - 1900 of 7434 citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and amplified with primers containing indexes for sequencing using Q5 High-Fidelity 2xMaster mix (New England Biolabs).
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bioRxiv - Cancer Biology 2023Quote: ... SYBR™ green and 0.5 µM of sample-specific P5/P7 barcoded primer mix (New England Biolabs). Bead clean-up and purification was performed using SPRI beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2023Quote: ... in combination with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs; New England BioLabs), following the manufacturers’ recommended protocols ...
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bioRxiv - Biophysics 2023Quote: ... 240 pmol of each primer (P1 and P2) and Phusion 2X mastermix with HF buffer (NEB, M0531S). The PCR mix was split into 100 μl aliquots and subjected to 35 cycles of 10 s 98 °C denaturation ...
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bioRxiv - Developmental Biology 2022Quote: ... The first strand cDNA was synthesized using oligo-dT primers and AMV-Reverse Transcriptase (New England Biolabs). The ziwi gene is expressed in the germ cells of both sexes and was used as germline control ...
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bioRxiv - Physiology 2023Quote: ... using either Luna Universal qPCR master mix for Sybr Green primers (New England Biolabs, Cat. No. M3003X) or Taqman Fast Advanced Master mix (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were indexed using NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (NEB #644OS/L). Library prep generated cDNA was quantified and analyzed using Agilent DNA chip and Qubit DNA dye ...
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bioRxiv - Immunology 2023Quote: ... 2.5 μL of 10 μM P7 primer and 0.5 μL of Phusion Polymerase (NEB, cat. no: M0530L). The qPCR was run using the following conditions ...
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bioRxiv - Genomics 2023Quote: ... with NEBNext Multiplex Oligos (96 Unique Dual Index Primer Pairs) for Illumina Barcodes (New England Biolabs, USA) and sequenced on an Illumina NovaSeq 6000 S2 150bp PE flow cell at the Duke Center for Genomic and Computational Biology Sequencing and Genomic Technologies Core.
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bioRxiv - Bioengineering 2023Quote: ... and 1.25 μl of 10 μM each primer using Phusion high-fidelity DNA polymerase (New England BioLabs). After PCR ...
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bioRxiv - Neuroscience 2023Quote: ... We generated cDNA with random hexamer primers and ProtoScript II Reverse Transcriptase (New England Biolabs, Hitchin, UK) or SuperScript III Reverse Transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2024Quote: ... 500 bp amplicons were generated using NEB VarSkip Short primer pools (Gautreau, 2021, NEB, Cat No. E7658) and Q5® HotStart MasterMix ...
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Fluoroquinolone-Specific Resistance Trajectories in E. coli and their Dependence on the SOS-ResponsebioRxiv - Microbiology 2024Quote: ... in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs; New England BioLabs). The samples were sequenced using a NextSeq 500/550 Mid Output V2.5 kit (Illumina).
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Aromatase in adipose tissue exerts an osteoprotective function in male mice via phosphate regulationbioRxiv - Physiology 2024Quote: ... Oligo-dT based primer was used for single-stranded synthesis and Second Strand Synthesis Module (NEB, #E6111) was used for double-stranded cDNA synthesis ...
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bioRxiv - Genomics 2024Quote: ... The oligo was double stranded with primer N80_rev for a single cycle using Phusion polymerase (NEB M0530). The double stranded N80 library was cloned into the XhoI site of yGPRA_pTpA using Gibson assembly and purified with SPRI beads ...
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bioRxiv - Microbiology 2024Quote: ... in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs; New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB, Cat. No. E6440S) and purified using the SPRIselect bead-based reagent (Beckman Coulter ...
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bioRxiv - Synthetic Biology 2024Quote: ... The KODΔ mutant was generated using primers PH_Delta_KOD_F1/R1 (Table S1) through iPCR and cloned into the final BL21(DE3) (NEB) strain following the same procedure as the library construction.
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bioRxiv - Genetics 2021Quote: ... PCR amplification was performed in a 10 µl reaction volume containing 1X PCR Buffer (New England Biolabs), 10 µM dNTP and 0.05 U Taq DNA polymerase (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We then performed PCR reactions at 12 cycles using Phusion High-Fidelity PCR Master Mix (NEB Biolabs) and primers that bind to common regions in the adaptors ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We then performed PCR reactions at 12 cycles using Phusion High-Fidelity PCR Master Mix (NEB Biolabs) and primers that bind to common regions in the adaptors ...
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bioRxiv - Microbiology 2019Quote: KLRC2 genotypes were determined by touchdown PCR using the Phusion High Fidelity PCR kit (New England Biolabs) using previously described methods40 ...
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... PCR was performed according to manufacturer’s protocol (Phusion High-Fidelity PCR kit, New England Biolabs, Ipswich, MA).
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bioRxiv - Genetics 2021Quote: ... The A-tailed PCR products were cleaned with Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB) and ligated into pGEM®-T Easy vector (Promega ...
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bioRxiv - Microbiology 2021Quote: ... All PCR steps were carried out using the Phusion High-Fidelity PCR Master Mix (New England Biolabs). The PCR products were electrophoresed on a 2% agarose gel and stained with SYBR Safe DNA gel stain ...
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bioRxiv - Systems Biology 2022Quote: ... PCR amplification was performed on the eluate with an NEBNext 2X High Fidelity PCR Mix (NEB, M0541S) N7 ...
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bioRxiv - Genetics 2022Quote: ... Phusion® High-Fidelity PCR Master Mix was used to prepare the PCR library (New England Biolabs). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... Random mutagenesis was performed using Error-Prone PCR (EP-PCR) with Taq DNA polymerase (New England Biolabs). PCR products and restriction digestion products were purified using a GeneJET gel extraction kit (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix (New England Biolabs). Mix same volume of 1×loading buffer (contained SYBR green ...
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bioRxiv - Genetics 2020Quote: ... DNA libraries were then prepared by PCR amplification using NEBNext High-Fidelity PCR kit (New England Biolabs) in the presence of barcoded PCR primers (sequences provided in Ref ...
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bioRxiv - Microbiology 2019Quote: ... All PCR reactions were carried out with Phusion®High-Fidelity PCR Master Mix (New England Biolabs). Mix same volume of 1X loading buffer (contained SYB green ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products with the expected size were column-purified with the Monarch PCR & DNA Cleanup Kit (NEB) and Sanger sequenced by GATC-Eurofins or Microsynth ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplification was performed using the Phusion High-Fidelity PCR Kit (New England Biolabs Inc, Ipswich, UK) and the final reaction contained 250 ng of template DNA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix (New England Biolabs), and the mixture PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... the scFv containing region from isolated plasmids was amplified by PCR using Q5 high fidelity PCR (NEB). Illumina NGS samples were prepped and run using the Illumina MiSeq v3 reagent kit following manufacturer’s protocols pooling four library samples per lane ...
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bioRxiv - Genetics 2021Quote: ... PCR amplification was done with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Genomics 2021Quote: ... first-level PCR reactions were performed using 1μl PCR-compatible lysate as a template for a 6.25μl Q5 (NEB) PCR reaction according to the manufacturer’s protocol (annealing temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Eluted DNA was enriched with PCR reaction using Fusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Genomics 2022Quote: ... The PCR product was cleaned using the Monarch® PCR clean up kit (New England Biolabs T1030S) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified pools were subjected to PCR product purification by Monarch PCR and DNA Cleanup Kit (NEB). The purified cDNAs were cloned by NEB PCR Cloning Kit and individual clones were isolated and sequenced (Azenta Life Sciences).
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bioRxiv - Immunology 2023Quote: ... 17 PCR cycles were performed using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532) with primers and template oligo each at a concentration of 10uM ...
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bioRxiv - Developmental Biology 2024Quote: ... After PCR amplification or plasmid digestion product was purified with Monarch PCR clean-up kit (NEB, #T1030s) and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche ...
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bioRxiv - Genetics 2023Quote: ... The purified DNA was then amplified by PCR using NEBNext HiFi 2x PCR Master Mix (NEB M0541S), and the resulting library was purified using 1.3X SPRI purification (Omega Bio-Tek ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were amplified by PCR with NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs #174M0541S) for 9 cycles in 96-well Thermal cycler (Biorad ...
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bioRxiv - Cell Biology 2023Quote: ... Synthesized testis cDNAs were used for endpoint PCR or quantitative PCR using OneTaq 2X Master Mix (NEB) or with iTaq Universal SYBR Green Supermix (BIO-RAD) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR fragments of interest were treated with the same enzymes and cleaned by PCR cleanup (NEB). Linear plasmid pJAM2678 and each PCR product were ligated together in a reaction using T4 DNA ligase overnight at 16 °C to obtain plasmid pAKS202 that contained 150 bp upstream of the translation start site of hvo_B0047 cloned in front of bgaH ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplification was performed with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Genomics 2023Quote: ... The PCR products were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, no. T1030L). In vitro transcription of pegRNAs and gRNAs was performed using the HiScribe SP6 RNA Synthesis Kit (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... Preamp PCR: Beads were resuspended in 200 µl PCR mix (20 µl 5x phusion HF buffer (NEB); 1 µl Phusion DNA Polymerase (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... and PCRs were performed via Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB #M0531S). After PCR clean-up (Promega #A9281 ...