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Citations for New England Biolabs :
1401 - 1450 of 7434 citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Dual-index primers were conjugated with NEBNext Multiplex Oligos for Illumina (Set1, New England Biolabs). We pooled all libraries ...
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bioRxiv - Plant Biology 2024Quote: ... with primers listed in Supplementary Table S6and cut by BamHI and SalI (New England Biolabs). The DNA fragments were cloned into a pTV00 vector using T4 DNA ligase (Promega ...
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bioRxiv - Microbiology 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs; New England BioLabs) were used for creating a genomic DNA library ...
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bioRxiv - Neuroscience 2024Quote: ... we introduced mutations by primers and aligned by HiFi DNA Assembly Master Mix (NEB, E2621L). Ligated PCR products inserted into the pNL1.1 vector (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were column-purified (Monarch PCR & DNA Clean-up Kit, New England BioLabs) and submitted for Sanger sequencing (Genewiz ...
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bioRxiv - Immunology 2020Quote: ... Each PCR mix contained of 25µl of NEB 2x PCR Mix (New England Biolabs), 2.5µl of 25µM forward primer (Primer Ad1_noMX) ...
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bioRxiv - Bioengineering 2021Quote: ... Double stranded preamplifier synthesis template was synthesized by PCR using LongAmp PCR polymerase (NEB), non-phosphorylated forward primer ...
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bioRxiv - Immunology 2022Quote: ... and PCR amplified and barcoded with NEBNext High-Fidelity PCR Mix (New England Biolabs) and unique dual indexes (Illumina) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR indexing was performed using NEBNext High-Fidelity 2x PCR Master Mix (M0541S, NEB) and sequenced using a NextSeq platform (Illumina) ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were amplified by PCR using NEBNext High-Fidelity 2x PCR Master Mix (NEB) using PCR primers Ad1_noMX and Ad2_Barcode (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified (Monarch® PCR & DNA Cleanup Kit 5µg; New England BioLabs) and sequenced from both sides with Eurofins Genomics ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR was performed on plasmid DNA with Phusion High Fidelity PCR Kit (NEB). PCR products were converted to RNA using the T7 RNA Transcription Kit (NEB ...
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bioRxiv - Physiology 2020Quote: ... was amplified by PCR (Phusion High-Fidelity PCR Master Mix, NEB, Hitchin, Hertfordshire, UK) using specific oligonucleotide primers (5’-TCGCTACTGTTTCTCTCGCA-3’ and 5’-AAAGGCGTCAACAACTGCTT-3’) ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR product was purified using the Monarch PCR & DNA clean-up kit (NEB). All library preparation and sequencing was carried out at the Vanderbilt Technologies for Advanced Genomics (VANTAGE ...
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bioRxiv - Cell Biology 2022Quote: ... sgRNA inserts were PCR-amplified using NEBNext High Fidelity PCR Master Mix (NEB, M0541). The resulting PCR amplicons were purified using Ampure XP Beads and sequenced using the HiSeq 2500 system (Illumina ...
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bioRxiv - Bioengineering 2022Quote: ... PCR products were column-purified (Monarch PCR & DNA Clean-up Kit, New England BioLabs) and submitted for Sanger sequencing (Genewiz ...
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bioRxiv - Systems Biology 2024Quote: ... PCR products were spin-purified using the Monarch PCR & DNA Cleanup Kit (NEB T1030) or the DNA Clean & Concentrator-5 kit (Zymo Research ...
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bioRxiv - Immunology 2024Quote: ... and PCR amplified with NEBNext High Fidelity 2x PCR Master Mix (New England Biolabs). Following purification with the PCR Purification Kit (QIAGEN) ...
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bioRxiv - Molecular Biology 2023Quote: ... Linearized cDNA was PCR amplified using Phusion High-Fidelity PCR Master Mix (NEB, M0531S).
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bioRxiv - Immunology 2024Quote: ... and PCR amplified and barcoded with NEBNext High-Fidelity PCR Mix (New England Biolabs) and unique dual indexes (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: ... For PCRs we used Phusion High-Fiedlity PCR Master Mix with HF Buffer (NEB) and reactions were run as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA samples were PCR-amplified using standard Illumina adaptors and Q5 PCR mix (NEB) with 10-13 cycles (SMInputs ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were generated by two-step PCR utilizing Q5 polymerase (New England Biolabs) with an annealing temperature of 72°C and a 2-minute extension time ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were column-purified (Monarch PCR & DNA Clean-up Kit, New England BioLabs) and submitted for Sanger sequencing (Genewiz ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed utilizing the Phusion High-Fidelity PCR Master Mix (NEB, M0531L) to prevent any mutations ...
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bioRxiv - Genomics 2023Quote: ... DNA was amplified by PCR with NEBNext HiFi 2x PCR Master Mix (NEB M0541S) and library was purified by 1.3X SPRI purification (Omega Bio-Tek ...
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bioRxiv - Genetics 2023Quote: ... PCR reactions were performed with Phusion High-Fidelity PCR Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The VHH specific sequence was amplified using PCR (Phusion High Fidelity PCR kit, NEB) and correct overhangs were incorporated ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was performed using Phusion High-Fidelity PCR Master Mix (New English Biolabs, England). PCR products were quantified using 2% agarose gel electrophoresis ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PCR reactions contained 1X Phusion High-Fidelity PCR Master Mix (New England BioLabs Inc.), 0.5 μM primers (forward ...
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bioRxiv - Microbiology 2023Quote: ... the PCR reaction was purified using the Monarch PCR Cleanup Kit (New England Biolabs). DNA was sent for Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: PCR products were routinely purified using Monarch® PCR & DNA clean-up kit (NEB), or E.Z.N.A.® Cycle Pure Kit (Omega Bio-Tek ...
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bioRxiv - Genomics 2023Quote: ... PCR was performed with the Phusion High-Fidelity PCR kit (E0553L, New England BioLabs) with the following cycling conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR was performed using NEBNext® High-Fidelity 2X PCR Master Mix (NEB; M0541S). The purified PCR products were separated by a 4-20% TBE gel (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Amplicons were purified from PCR buffer using Monarch PCR cleanup kit (New England Biolabs) and submitted for Sanger sequencing (EtonBio).
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR reactions were performed using Q5 High-Fidelity 2X PCR Master Mix (NEB) with optimized Tm and the PCR products were detected by 1% agarose gel.
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were purified using the Monarch PCR and DNA Cleanup Kit (NEB, #T1030L) prior to Sanger sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were generated via PCR amplification using NEBNext HighFidelity 2× PCR Master Mix (NEB). The resultant libraries were purified again using the MinElute PCR Purification Kit (Qiagen) ...
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bioRxiv - Genetics 2024Quote: ... PCR amplifications were performed using Phusion High-Fidelity PCR kit (New England Biolabs #M0530L) according to manufacturer’s protocol using 50 ng template DNA per 100µL reaction and 18 cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... during 8 PCR cycles with NEBNext HiFi PCR Master mix (New England Biolabs, #M0541S). Fragments of DNA 300 and 800 bp were then using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Genetics 2021Quote: ... 1 μg of total Trizol-purified RNA purified was used for library preparation using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB) with libraries indexed in 12 cycles PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified small RNA was subject to library prep using NEBNext Multiplex Small RNA Sample Prep Set for Illumina (NEB E7330) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... ChIP DNA was subject to library prep using NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (NEB E6240) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA was cleaved from the beads with DpnII and libraries were prepared for sequencing using the NEBNext DNA Library Prep Master Mix Set for Illumina (New England BioLabs).
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bioRxiv - Genomics 2020Quote: ... Libraries were prepared from 1000 ng total RNA using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) by the Transcriptional Regulation and Expression Facility at Cornell University.
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bioRxiv - Genomics 2020Quote: ... single-indexed libraries using the NEBNext® DNA Library Prep Master Mix Set for 454™ (E6070, New England Biolabs) and Illumina-specific adaptors52 following established protocols52–54 ...
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bioRxiv - Neuroscience 2020Quote: Six µL of each sample were used for library preparation with the NEBNext Multiplex Small RNA Sample Preparation Set for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... barcoded sequencing libraries were constructed using the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent (New England Biolabs), and templated using the Ion PI Hi-Q Chef kit (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... and set up in an overnight in vitro transcription reaction (HiScribe T7 Quick High Yield RNA Synthesis kit, New England BioLabs). The resulting RNA was purified using Silane beads (Life Technologies ...
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bioRxiv - Plant Biology 2019Quote: ... Indexed sRNA libraries were constructed from these enriched sRNA fractions with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturer’s instructions ...