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Citations for New England Biolabs :
1751 - 1800 of 7434 citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: The small RNA sequencing libraries were constructed using 3 μg total RNA per sample and NEB Next® Multiplex Small RNA Library Prep Set for Illumina® (NEB) following the manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2019Quote: ... 2x 1-5 µg of DNA were adaptor ligated and indexed using the NEBNext DNA library Prep Reagent Set (New England Biolabs: E6040S/L) and NEBNext Multiplex Oligos for Illumina Primer sets 1 (New England ...
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bioRxiv - Developmental Biology 2019Quote: ... we used index pair combinations reported for the New England Biolabs NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Pairs) set (catalog number E6440, New England Biolabs, Beverly, Massachusetts), replacing the (n)8 sequences above with the appropriate i5 and i7 barcode ...
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bioRxiv - Genomics 2019Quote: Libraries were prepared using half total volume of eluted ChIP DNA and NEBNext® DNA Library Prep Master Mix Set and Multiplex Oligos for Illumina® (New England Biolabs). Library quality was assessed using Bioanalyzer 2100 High Sensitivity DNA Gels (Agilent) ...
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bioRxiv - Immunology 2020Quote: ... libraries were prepared using NEBNext® Ultra II DNA Library Prep Master Mix Set and Multiplex Oligos for Illumina® (New England Biolabs). Library quality was assessed using Bioanalyzer 2100 High Sensitivity DNA Gels (Agilent) ...
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bioRxiv - Cancer Biology 2022Quote: The EV-RNA was compiled into cDNA libraries using a NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible) (Cat No.: E7330, NEB, Ipswich, USA) following the manufacturer’ s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... These two combined samples were ethanol precipitated and used to generate male and female L1 small RNA libraries using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, MA, USA). Indexed libraries were size selected for sRNAs (<150nt ...
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bioRxiv - Genetics 2022Quote: ... The tRNAs were re-phosphorylated and converted to small RNA sequencing libraries using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs). Size selection of 140-155 bp PCR amplified fragments (corresponding to 19-35 nt tRNA fragments size range ...
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bioRxiv - Genetics 2019Quote: ... 2× 1-5 µg of DNA were adaptor ligated and indexed using the NEBNext DNA library Prep Reagent Set (New England Biolabs: E6040S/L) and NEBNext Multiplex Oligos for Illumina Primer sets 1 (New England ...
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Investigations on regulation of miRNAs in rice reveal [Ca2+]cyt signal transduction regulated miRNAsbioRxiv - Plant Biology 2021Quote: Small RNA libraries were prepared with 2 μg of enriched small RNA using NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible) (New England Biolabs) according to the manufacturer’s protocol in biological dupicates ...
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bioRxiv - Genomics 2021Quote: Library preparation was performed with 150 ng of starting material using NEBNext Fast DNA Library Prep Set for Ion Torrent (New England Biolabs, Massachusetts, USA) and following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... the adapter ligation was performed using 2.5 µl of NEBNext® Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1 - NEB, cat# E7416S); the post-adapter ligation purification was performed using 50 µl of purification beads and 50 µl of purification beads’ buffer ...
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bioRxiv - Genetics 2021Quote: Sequencing libraries were prepared from either 500 ng total RNA or 3 µl TraPR-enriched [80] sRNA with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (E7300; NEB, Ipswich, Massachusetts) according to the manufacturer’s protocol ...
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NSC-derived exosomes enhance therapeutic effects of NSC transplantation on cerebral ischemia in micebioRxiv - Neuroscience 2022Quote: Sequencing libraries of miRNAs of NSC-derived exosomes were produced using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, United States) following the previous report (Cai et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred nanograms of RNA was used to generate sequencing libraries using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® Kit (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Libraries were prepared using the NEBNext® DNA Library Prep Reagent Set for Illumina® kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The obtained cDNA was used to prepare a NEBNext DNA Library Prep Set for Ion Torrent (New England Biolabs, Ipswich, MA, USA) and barcoded with the use of Ion Code Barcode Adapters (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed from 800 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, New England Biolabs, NEB-E7560S) and the small RNA fraction was sequenced on the NextSeq 500 System (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... libraries were constructed from 1000 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, NEB-E7560S, New England Biolabs) and the small RNA fraction sequenced (NextSeq 500/550 High Output Kit v2.5 75 Cycles ...
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bioRxiv - Molecular Biology 2023Quote: Linker ligation at 3’ ends was set up per sample after washes following alkaline phosphatase treatment by preparing a T4 RNA ligase 1 (NEB, cat. #M0204S) reaction in 40μL following the manufacturers’ instructions with 100pmol of radiolabeled L32 RNA linker ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed from 300 ng (liver) or 200 ng (hypothalamus) total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, New England Biolabs, NEB-E7560S) and the small RNA fraction was sequenced on the NextSeq 500 System (Illumina ...
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bioRxiv - Biochemistry 2024Quote: ... Digestion was set up to remove the 6X-His-tag by incubating with either homemade or store bought (NEB, Cat. no: P8070L) enterokinase in the presence of 10mM CaCl2 at 22°C for 16h ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were generated using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina (E7330S, New England BioLabs, Ltd., USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Molecular Biology 2024Quote: ... All treated RNA samples were used for library preparation using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB #E7300/7580) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Purified RNA was treated with PNK4 before being used for cDNA synthesis using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® Kit (NEB). Obtained cDNA template was amplified by PCR using the LongAmp® Taq 2XMaster Mix (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... and then 1μg RNA was converted to cDNA using Protoscript II RT (M0368S, New England Biolabs) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... Particle titers were determined by isolation of genomic RNA and quantitation by RT-qPCR (NEB # E3031) using an Applied Biosystems vii7A™ Real-Time PCR system ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were prepared using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Quantitative RT-PCR was performed with an Mx3000P qPCR system (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... 1 µg of RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (NEB) with and without reverse transcriptase (RT) ...
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bioRxiv - Cancer Biology 2020Quote: ... mRNA expression was determined using SYBR green Luna One Step RT-qPCR Kit (New England BioLabs) on a C1000 Touch (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA synthesis was carried out using LunaScript™ RT SuperMix Kit (New England Biolabs, USA), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... For reverse transcription (RT) 1 µg RNA was digested with 2 U of DNase I (NEB). After heat inactivation of the DNase at 70 °C for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... Filtered (0.45 μm) supernatants were treated with 1 U/ml DNAse I (NEB; 1 h, RT) and purified through a 20% sucrose cushion (2 h ...
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bioRxiv - Microbiology 2022Quote: ... we followed the protocol described by the Template Switching RT Enzyme Mix provider (New England Biolabs) with a few modifications (Supplementary Materials and Methods) ...
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bioRxiv - Cancer Biology 2022Quote: ... All ligation reactions were performed at room temperature (RT) for 30 min using T4 ligase (NEB). Bacterial transformation was performed following standard protocols using Stellar competent cells (Takara) ...
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bioRxiv - Immunology 2021Quote: ... 500 ng total RNA was used with the Template Switching RT Enzyme Mix (NEB Ipswich, USA) to generate 5’ RACE cDNA ...
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bioRxiv - Immunology 2020Quote: ... Real time qPCR was performed using the Luna Universal Probe One-Step RT-qPCR Kit (NEB) run on a QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... We prepared cDNA from 1μg of extracted RNA using LunaScript® RT SuperMix Kit (NEB, #E3010). No Template and No Reverse Transcriptase controls (NTC and NRT ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression was determined using SYBR green Luna One Step RT-qPCR Kit (New England BioLabs) on a C1000 Touch (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: One μg of RNA was reverse transcribed using the LunaScript RT SuperMix Kit (New England Biolabs) according to the manual ...
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bioRxiv - Molecular Biology 2022Quote: ... subsequently used to generate cDNA with LunaScript RT SuperMix Kit (New England Biolabs, catalog no. E3010S). Real-time PCR was performed using SYBR green gene expression assays (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR reactions were performed using Luna® Universal qPCR Master Mix (New England Biolabs #M3003). Primers for qPCR reactions are shown in Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of total RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instructions and 1 µl cDNA was used as a template for a 10 µl qPCR reaction ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (NEB) with and without reverse-transcriptase ...
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bioRxiv - Pathology 2024Quote: ... The isolated RNA was then reverse transcribed into first-strand cDNA using LunaScript RT SuperMix (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Complementary DNA (cDNA) was generated for each replicate using the LunaScript RT Supermix (New England Biolabs) based on the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... reverse transcription from mRNA was performed using the LunaScript® RT SuperMix Kit (New England BioLabs) following standard protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-LAMP reactions were performed using the WarmStart® LAMP Kit (New England Biolabs Cat # E1700) following the manufacturer’s instructions without addition of the fluorescent dye ...
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bioRxiv - Immunology 2023Quote: Reverse transcriptase qPCR was performed using Luna Universal Probe One-Step RT-qPCR Kit (NEB, # E3006L). The following predesigned primers and probe sets for each target and the housekeeping gene GAPDH were purchased from IDT:
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bioRxiv - Physiology 2023Quote: ... and 1µg RNA was transcribed into cDNA using LunaScript RT Super Mix (E3010L, New England Biolabs). Afterwards the mRNA levels were determined by qPCR using SYBR Green (Luna Universal qPCR Master Mix ...