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Citations for New England Biolabs :
1451 - 1500 of 7434 citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2019Quote: ... Transformation reactions were set up using 5ul of ligation reaction and 50ul chemically competent E.coli cells (New England Biolabs # C3040I) and performed as per manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... 10 ug of gel-purified mononucleosome DNA was prepared for high-throughput sequencing using the NEBNext DNA Library Prep Master Mix Set for Illumina (NEB). Sequencing was performed by the University of Oregon Genomics and Cell Characterization Core Facility.
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bioRxiv - Systems Biology 2020Quote: ... we first phosphorylated the 5’ ends of each probe set by combining 4 μL of the pooled oligos with 1 μL T4 PNK (NEB), 20 μL T7 DNA ligase reaction buffer (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... for ChIP-Seq library preparation using the New England Biolabs NEBNext ChIP-Seq Library Prep Reagent Set for Illumina (New England BioLabs) following the manufacturer’s recommended protocol ...
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bioRxiv - Cell Biology 2020Quote: ... and 7 μl were used as input for NGS library preparation using the NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs). Sequencing was performed in a MiSeq benchtop sequencer for 200 cycles ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Transcription reactions were set up with RNA NTPs (5 mM each ATP, CTP, UTP, 9 mM GTP) (NEB, Ipswitch, MA), 0.004 U/µL Thermostable Inorganic Pyrophosphatase (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... ChIP and input DNA libraries were prepared for amplification by using the NEBNext Ultra II ChIP-seq Library Prep Master Mix Set for Illumina (NEB) following manufacturer’s protocols with no modifications ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: Library preparation from co-immunoprecipitated RNA samples were carried out with NEBNext Multiplex Small RNA Library Prep Set (New England Biolabs) that allow sequencing in Illumina platforms ...
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bioRxiv - Plant Biology 2021Quote: ... miR158 OE and pseudo-PPR OE (one of these libraries failed the quality control).Libraries were prepared using NEB Next Small RNA Library Prep Set (New England Biolabs) and sequencing was carried out using Illumina HiSeq 4000 in single-end mode by outsourcing ...
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bioRxiv - Microbiology 2021Quote: ... Indexed sRNA libraries were constructed from these sRNA fractions with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Small RNA deep sequencing libraries were generated using the NEBNext Small RNA Library Prep Set for Illumina (E7560, New England Biolabs), using 1 µg RNA as input ...
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bioRxiv - Developmental Biology 2021Quote: ... sRNA libraries for Ziwi-IP samples were prepared using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, E7300S) and sequenced on HiSeq 50bp single-end sequencing platform.
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bioRxiv - Cancer Biology 2021Quote: ... For cloning the oligo pool into the appropriate lentiviral backbone the following reaction was set up: 5 μl 10x Cutsmart buffer (NEB), 1 mM DTT (final) ...
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bioRxiv - Molecular Biology 2020Quote: ... The NEBNext® Small RNA Library Prep Set for Illumina® was used for library preparation (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2020Quote: ... The NEBNext® Small RNA Library Prep Set for Illumina® was used for library preparation (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was sheared mechanically with the Covaris S220 and processed with the NEBNext DNA Library Prep Master Mix Set for Illumina (NEB) before submitting for sequencing on an Illumina MiSeq platform generating paired end 150 bp reads ...
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bioRxiv - Genetics 2019Quote: ... Second-strand cDNA synthesis was used to assemble a library and sequenced following the manufacturer’s instructions (NEB Next DNA Library Prep Master Mix Set for Illumina; NEB E6040L). Sequenced reads were aligned to the human reference genome hg19 using TopHat v2.1.0 with the ‘--no-coverage-search --read-realign-edit-dist 0’ option selected (17) ...
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bioRxiv - Molecular Biology 2019Quote: Sequencing libraries were prepared using NEBNext® Fast DNA Library Prep Set for Ion Torrent(tm) Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... fragmented and converted into 3C library using NEBNext DNA Library Prep Master Mix Set for Illumina (NEB E6040, M0543L, E7335S). After indexation with NEBNext multiplex oligos ...
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bioRxiv - Genomics 2019Quote: ... Libraries were constructed from 120 ng of purified small RNAs using the NEBNext Multiplex Small RNA Library Preparation Set for Illumina (New England Biolabs). Single-end sequencing (50 nt ...
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bioRxiv - Genomics 2019Quote: ... extracted RNA without rRNA depletion was directly subjected to library preparation with the NEBNext Small RNA Library Prep Set kit (New England Biolabs) and sequenced on a HiSeq2000 (Illumina ...
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bioRxiv - Genomics 2019Quote: ... We prepared WT libraries using the NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs: E6040S/L) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... ChIP-seq libraries were prepared using the NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (NEB, product E6240). Libraries were size selected using AMPure XP beads (Beckman ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified small RNA was subject to library prep using NEBNext Multiplex Small RNA Sample Prep Set for Illumina (NEB E7330) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... dA-tailed and ligated to adapter_STARR1/adapter_STARR2 to be compatible with Illumina sequencing according to the NEBnext DNA library prep master mix set for Illumina (New England Biolabs, oligonucleotide sequences shown in Supplementary Table 6) ...
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bioRxiv - Microbiology 2021Quote: ... NGS libraries were generated using the NEBNext Fast DNA Library Prep Set for Ion Torrent (New England Biolabs, Hitchin, UK). Pooled samples were size selected with the LabChip XT (LabChip XT DNA 300 Assay Kit ...
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bioRxiv - Biochemistry 2021Quote: ... The ligation reaction was then set up by combining the cDNA and adapter mixture with 1X T4 DNA Ligase Buffer (NEB), 500 mM Betaine ...
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bioRxiv - Cell Biology 2020Quote: ... Generation of double stranded cDNA and library construction were performed using NEBNext® mRNA Sample Prep Reagent Set 1 (NEB). Ligation of adapters was performed using Adapters prepared at the WTCHG according to the Illumina design (Multiplexing Sample Preparation Oliogonucleotide Kit) ...
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bioRxiv - Microbiology 2020Quote: A cDNA library of the CLIP samples was prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (#E7300, NEB) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ChIP seq libraries were prepared using NEBNext ChIP-Seq Library Prep Reagent Set for Illumina (New England BioLabs, Cat # E6240). High throughput sequencing was performed on Illumina HiSeq 2500 for single-end 50 bp reads or Illumina NovaSeq 6000 for paired-end 50 bp reads.
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bioRxiv - Immunology 2020Quote: ... 3 μg of ribosome-protected mRNA was used to prepare a library with the NEBNext Small RNA Library Prep Set (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: A total of 200 ng of total RNA were used to generate barcoded miRNA-seq libraries using the NEBNext Multiplex SmallRNA Library Prep Set for Illumina (New England Biolabs). Briefly ...
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bioRxiv - Genomics 2020Quote: ... 1 μg of the input and all the immunoprecipitated DNA was converted into sequencing libraries using the NEBNext DNA library prep master mix set (NEB) following manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: ... The isolated mRNA was then used to prepare sequencing libraries using NEBNext Ultra II Non-directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (Index sets 1 and 2) (NEB). Next ...
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bioRxiv - Molecular Biology 2021Quote: ... Fragments of 10 - 40 nt for partially hydrolyzed and re-phosphorylated tRNAs were converted to small RNA sequencing libraries using NEBNext multiplex small RNA library prep set (NEB) for Illumina kit ...
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bioRxiv - Biophysics 2022Quote: ... the assembled sequences were transferred to the target plasmids (WT, MUT) by using a different set of type IIS enzymes (BbvI, New England Biolabs #R0173 ...
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bioRxiv - Genetics 2022Quote: ... Purified small RNA was subject to library prep using NEBNext Multiplex Small RNA Sample Prep Set for Illumina (NEB E7330). Adaptor-ligated ...
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bioRxiv - Molecular Biology 2022Quote: ... purified DNA fragments were subjected to library preparation with NEB Next Ultra II and NEB Multiplex Oligo Set I/II as per manufacturer (New England Biolabs) protocol without size selection ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2) library for miRNAs detection utilizing NEBNext® Small RNA Library Prep Set for Illumina (New England Biolabs (UK) Ltd ...
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bioRxiv - Molecular Biology 2024Quote: ... the libraries were firstly constructed using the NEB Next® Multiplex Small RNA Library Prep Set for Illumina® (NEB). Total RNA was then ligated to adaptors at the 3’ end by NEB 3’ SR adaptor and 5’ end by T4 RNA ligase ...
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bioRxiv - Molecular Biology 2024Quote: ... and sequenced on a NextSeq 2000 instrument (Srp2-HTP & Dbp2-HTP) or with the NEBNext Fast DNA Library Prep Set for Ion Torrent Kit (NEB) and sequenced on an Ion Torrent Proton (Life Technologies ...
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bioRxiv - Genomics 2024Quote: The libraries for sequencing were made with NEBNext DNA library Prep Master Mix Set for 454 (E6070, New England Biolabs) and with Illumina-specific adaptors [54] using established protocols [54–56] ...
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bioRxiv - Plant Biology 2023Quote: Small RNA libraries were prepared using the NEBNext® Small RNA Library Prep Set for Illumina® (New England Biolabs), and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2023Quote: ... To remove RNA secondary structure and anneal the mRNA capture primer 1 μL of tagged random hexamer (100 μM) and 0.5 μL of 10 mM dNTPs (dNTP solution set NEB - N0446S) were added to the sample ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The small RNA library preparation was done with 1 µg of total RNA used as input into the NEBNext Small RNA Library Prep Set for Illumina (New England BioLabs) and an AMPure XP Bead (Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: ... we constructed the NGS library using the products from the last step following the NEBNext Small RNA Library Prep Set for Illumina (New England BioLabs) protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... to construct a set of plasmids (pOSV00169, pOSV00205, pOSV00206, pOSV00207, pOS00208, pOSV00292, pOSV00459 and pOSV00475) using restriction enzymes BamHI-HF (New England Biolabs) and EcoRI-HF (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were constructed using approximately 6 ng of total EV RNA as input material for the RNA sample preparations using the Small RNA Library Prep Set for Illumina kit (New England BioLabs) following manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... libraries were constructed from 1000 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, NEB-E7560S, New England Biolabs) and the small RNA fraction sequenced (NextSeq 500/550 High Output Kit v2.5 75 Cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting footprints were then used to generate sequencing libraries using the NEBNext Small RNA Library Prep Set for Illumina kit (New England BioLabs). Library quality was assessed using High Sensitivity DNA Chips with the Agilent 2100 Bioanalyzer System (Agilent Technologies).