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Citations for New England Biolabs :
1601 - 1650 of 2029 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Hi-C libraries were treated with T4 DNA Polymerase (New England Biolabs, catalog No. M0203) to remove biotin from unligated ends ...
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bioRxiv - Evolutionary Biology 2024Quote: ... pBBR1-E and pBBR-EV were extracted using Monarch Plasmid Miniprep kit (#T1010; NEB) and following supplier’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and amplified/tagged in two steps using NEBnext High-Fidelity 2x PCR master mix (New England Biolabs). Amplified DNA was purified twice with 1.8 volumes of NucleoMag NGS Clean-up and Size Select beads (Macherey Nagel) ...
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bioRxiv - Biophysics 2020Quote: mNG-tagged ADRβ2 and EGFR were generating by cloning into the pSNAPf-ADRβ2 backbone (New England Biolabs). mNG was amplified by PCR from mNG-C1 and placed between the EcoRI and SbfI sites of pSNAPf-ADRβ2 (replacing the SNAP tag ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli K-12 strain NEB DH-10 Beta (New England Biolabs, MA, USA) unless stated otherwise ...
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bioRxiv - Biophysics 2021Quote: ... coli DNA was performed identically using Nb.BsrDI and 1X CutSmart (New England Biolabs) substituted for the two enzymes used to prepare λ-DNA ...
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bioRxiv - Biochemistry 2021Quote: Plasmid propagation and subcloning was performed using Escherichia coli DH10β competent cells (NEB). The codon optimized GTL gBlock (Supporting Information Table 1 ...
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bioRxiv - Biophysics 2021Quote: ... where GRN-3 was expressed in Escherichia coli SHuffle cells (New England Biolabs) while other GRNs were expressed in Origami 2 DE3 cells (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... coli SHuffle T7 Express lysY and the restriction enzymes were purchased from NEB Japan ...
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bioRxiv - Plant Biology 2021Quote: ... constructs were separately introduced into the Escherichia coli (Express Competent cells C2523H, NEB). The purification was performed using amylose resin (NEB ...
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bioRxiv - Bioengineering 2021Quote: This study used the Escherichia coli strains NEB10β (New England Biolabs, Ipswich, MA), Nissle 1917 as well as all strains listed in Table 1 ...
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bioRxiv - Biochemistry 2019Quote: NHERF1 PDZ2-DTRL was expressed in Escherichia coli BL21 (DE3) (New England BioLabs). Cultures in auto-induction media (12 g/L tryptone ...
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bioRxiv - Biochemistry 2020Quote: All proteins were expressed in Escherichia coli NiCo21 (DE3) cells (New England BioLabs). To co-express multiple proteins ...
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bioRxiv - Molecular Biology 2022Quote: ... pHia5ET was transformed into T7 Express lysY/Iq Escherichia coli cells (NEB C3013I). Overnight cultures were added to two 1 L cultures of LB medium supplemented with 50 µg/mL kanamycin and grown with shaking at 37°C to an OD600 of 0.8-1.0 ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells following the recommended High Efficiency Transformation Protocol (C2987, New England Biolabs) described by the commercial strain provider ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids (Table S3) were propagated in Escherichia coli DH5α cells (NEB, United Kingdom) by growing cells in LB media without NaCl (Formedium ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cells were subjected to high fidelity Q5 Polymerase (NEB, Singapore; Cat: M0491L) PCR to generate 200 bp templates of the Ag43 signal peptide (Figure 1A) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli and grown at 30°C to ensure construct stability (New England Biolabs). Plasmid clones were picked and inoculated into liquid LB media for overnight culture followed by miniprep using the PureLink Plasmid MiniPrep Kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Total RNA samples were polyadenylated using Escherichia coli poly(A) polymerase (NEB, M0276L) for 5 min and purified by GeneJET RNA Cleanup and Concentration Micro Kit (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: ... coli (MG1655) genome containing the rrnC operon was amplified by PCR (Phusion, NEB) using the following primers:
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bioRxiv - Microbiology 2023Quote: ... coli (High Efficiency) (Cat. No. C3019H, New England BioLabs, Inc, Ipswich, MA, USA) following the manufacturer’s instructions with a recovery time of either 1 hour (standard time ...
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bioRxiv - Molecular Biology 2023Quote: Both HURP plasmids were transformed into Rosetta2 (DE3) Escherichia coli cells (NEB: C29871) and grown in 2 L of LB media containing 50 μg/mL ampicillin and 25 μg/mL chloramphenicol at 37 °C in a shaking incubator (200 rpm) ...
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bioRxiv - Microbiology 2023Quote: ... coli overnight cultures using a Monarch plasmid miniprep kit (New England Biolabs, USA) and concentration was quantified by NanoDrop (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli were performed with the Monarch® Plasmid Miniprep Kit (New England Biolabs).
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 T7 Express lysY/Iq cells (New England BioLabs, catalog no. C3013) and purified as previously described ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... coli DH5alpha colonies using OneTaq® 2X Master Mix (New England Biolabs, USA) to confirm the correct insertions and further sequenced with Sanger method (Eurofins Scientific ...
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bioRxiv - Cell Biology 2024Quote: Proteins were expressed in Escherichia coli T7 Express lysY/Iqcells (New England Biolabs) in 2×TY medium at 37°C to an OD600 of 0.8–1.2 before inducing protein expression by addition of 0.4 mM isopropyl β-D-thiogalactopyranoside (IPTG) ...
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bioRxiv - Genomics 2023Quote: ... pHia5ET was transformed into T7 Express lysY/Iq Escherichia coli cells (NEB C3013I). Overnight cultures were added to two 1 L cultures of LB medium supplemented with 50 µg/mL kanamycin and grown with shaking at 37°C to an OD600 of 0.8-1.0 ...
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bioRxiv - Molecular Biology 2024Quote: Plasmid DNA was purified from Escherichia coli DH5α competent cells (New England Biolabs) using a Qiagen Miniprep kit ...
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bioRxiv - Molecular Biology 2019Quote: ... or without 350 units of recombinant GSK3β (rabbit skeletal muscle) (New England BioLabs) and 1X hot kinase buffer (50mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant MBP-Megator fragments were purified with amylose magnetic beads (New England Biolabs) and eluted in Column Buffer supplemented with 10mM Maltose ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were affinity-purified using amylose resin using the manufacturer’s protocol (NEB) and eluted with 20 mM maltose ...
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bioRxiv - Molecular Biology 2019Quote: ... 15μL of myc-eIF6-bound beads were incubated with/without recombinant GSK3β (NEB) and suspended in cold kinase buffer and incubated at 30°C for 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM CaCl2) at room temperature in the presence of recombinant enteropeptidase (NEB) at 13 U enzyme per mg TMPRSS2 zymogen ...
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bioRxiv - Molecular Biology 2021Quote: ... The recombinant protein was sequentially purified using an amylose column (New England Biolabs) followed by a nickel-nitrilotriacetic acid column (Qiagen ...
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bioRxiv - Biophysics 2022Quote: Recombinant wild-type human histone H1.0 was used (H1; New England Biolabs M2501S). ProTαC and unlabeled ProTα were prepared as previously described (26) ...
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bioRxiv - Genomics 2024Quote: ... 200 µg/mL molecular biology grade recombinant albumin (rAlbumin) (New England Biolabs B9200S), 0.8 U/µL RiboLock RNase inhibitor (Thermo Fisher Scientific EO0384) ...
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bioRxiv - Immunology 2021Quote: ... In-vitro-transcription was carried out using the Hi-Scribe RNA transcription kit (New England BioLabs). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... concentrations measured using Qubit 1X dsDNA HS Assay) was treated with 5U of RNAse HI (NEB) in RNAse HI buffer for 30 min at 37°C and then nucleic acids were purified with GeneJET Gel Extraction and DNA cleanup micro kit (General cleanup protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... ENTR and DEST vectors were generated using Gibson assembly (NEB Builder Hi-Fi DNA assembly #E2621S) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μg of His-PKR was dephosphorylated using 3,200 units of λ-PPase (New England Biolabs) in 200 μl reaction buffer (50 mM HEPES ...
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bioRxiv - Plant Biology 2022Quote: ... The HIS tag was removed from the mature sequence of AgLTP24 with enterokinase (NEB, Evry, France) for 16 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: Plasmids for the trans-Tango components were generated using the Hi-Fi DNA Assembly (NEB #E5520S), BP Gateway Cloning (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... 750-basepair regions flanking each gene were amplified by PCR and Hi-Fi DNA Assembly (NEB) was used to clone into pExchange-tdk ...
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bioRxiv - Cancer Biology 2023Quote: ... Hi-C single-index library preparation was performed as previously described56 using MboI (New England Biolabs) restriction enzyme.
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bioRxiv - Genomics 2024Quote: ... The Hi-C libraries were amplified for 11–15 cycles with Q5 master mix (NEB, M0492L) following the operation manual ...
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bioRxiv - Microbiology 2024Quote: ... Tags (6x His or HA) were introduced using site-directed mutagenesis with KLD enzyme mix (NEB).
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bioRxiv - Synthetic Biology 2021Quote: ... The tagged pore variant plasmids were constructed by Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (NEB), where the variant encapsulin genes were individually amplified by PCR from the untagged pore variant plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG-tagged GPI-anchored PROM1- EX1 was generated by the DNA assembly method (#E2621, NEB, Ipswich, MA, USA). The GPI- anchor signal sequence from pCAG:GPI-GFP (#32601 ...
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bioRxiv - Cell Biology 2020Quote: ... The Myc-tagged Nix-S212A and Nix-S212D were generated using a Q5 mutagenesis kit (New England Biolabs). The lentiviral shNix (Addgene #100770 ...