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Citations for New England Biolabs :
1651 - 1700 of 2029 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... The Hi-C libraries were amplified for 11–15 cycles with Q5 master mix (NEB, M0492L) following the operation manual ...
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bioRxiv - Microbiology 2024Quote: ... Tags (6x His or HA) were introduced using site-directed mutagenesis with KLD enzyme mix (NEB).
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bioRxiv - Neuroscience 2021Quote: ... C or E antibodies and the immunoprecipitates were treated with PNGase F (New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2020Quote: ... was cloned into pET28a and expressed in Escherichia coli ER2566 cells (New England Biolabs, fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS ...
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bioRxiv - Bioengineering 2022Quote: ... Sequence verified plasmids were transformed into BL21(DE3) Escherichia coli cells (New England Biolabs) and plated on LB medium supplemented with 100 μg mL−1 ampicillin ...
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bioRxiv - Synthetic Biology 2019Quote: The bacterial strains used in this study includes Escherichia coli DH5α (New England Biolabs), Escherichia coli Nissle 1917 (isolated from probiotic Mutaflor capsule) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid cloning and multiplication were performed in Escherichia coli DH5α (New England Biolabs, USA). E ...
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bioRxiv - Biochemistry 2022Quote: ... All other proteins were expressed in Escherichia coli BL21(DE3) cells (New England BioLabs).
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bioRxiv - Bioengineering 2021Quote: ... coli cell libraries using M13K07 helper phage (New England Biolabs, Whitby, Canada, Cat#N0315S) as described in 81 and used for selection experiments described below.
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bioRxiv - Microbiology 2021Quote: ... coli JP313 ΔtolC strains using a Q5 high-fidelity DNA PCR (New England Biolabs). The primers used for these reactions were “EM029-TMKF1” (5’-ATGGCAAACTTTCTCGTG-3’ ...
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bioRxiv - Microbiology 2022Quote: ... The Escherichia coli strain used for routine cloning was NEB 5α (New England Biolabs). The plasmids used in this article are listed below (Table S2) ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmids were amplified by transformation into chemically competent Escherichia coli DH5α (New England Biolabs), which was plated onto LB agar (10 g/L tryptone ...
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bioRxiv - Molecular Biology 2021Quote: ... The subcloned AGO plasmids were transformed into Escherichia coli BL21 (DE3) (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: All cell extracts for CFE were prepared with Escherichia coli BL21 Star(DE3) (NEB).21 These cells were grown ...
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bioRxiv - Plant Biology 2021Quote: ... coli BL21(DE3) and purified by affinity chromatography using amylose resin (New England Biolabs). Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel) ...
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bioRxiv - Microbiology 2021Quote: ... coli transformations were performed using NEB 5-alpha chemically competent cells (New England BioLabs). E ...
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bioRxiv - Microbiology 2022Quote: ... coli host strain using the Monarch® Plasmid Miniprep Kit (NEB, Ipswich, MA, USA). The extracted plasmid was linearized with restriction enzyme BamHI (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... coli PURExpress system were generated from the T7 PURExpress plasmid (New England Biolabs, USA). pSpCas9(BB)-2A-Puro (PX459 ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... coli following the NEBuilder HiFi DNA Assembly protocol (New England Biolabs; Ipswich, United States). Knockouts of A ...
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bioRxiv - Genomics 2023Quote: ... and second-strand cDNA using Escherichia coli DNA ligase and polymerase (New England Biolabs), followed by a clean up step (1.8:1 beads to sample ratio) ...
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bioRxiv - Bioengineering 2023Quote: ... coli BL21(DE3) competent cells for expression following the manufacturer’s instructions (New England Biolabs). Colonies harbouring pET-rHl ...
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DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein EngineeringbioRxiv - Bioengineering 2023Quote: The Gibson products are transformed into T7 express competent Escherichia coli cells (NEB C2566). Individual colonies are grown in 600 μL Luria Broth (RPI L24400 ...
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bioRxiv - Biochemistry 2023Quote: ... were transformed with Lpg2248 (LotA) plasmids and T7 express Escherichia coli competent cells (NEB) were transformed with Lpg1621 (LotB ...
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bioRxiv - Synthetic Biology 2024Quote: ... integration vector backbones were transformed into chemically competent NEB Stable Escherichia coli (NEB #C3040H), and cells were grown at 30 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... coli Exonuclease III to generate ssDNA pools according to manufacturer’s instructions (New England Biolabs). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... coli cells using the Q5 mutagenesis kit in accordance with the manufacturer’s instructions (NEB). For FACS rescue experiments with WT-CHC22 or 11TD-CHC22 ...
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bioRxiv - Plant Biology 2019Quote: ... Recombinant proteins were purified using gravity flow columns with amylose resin (New England Biolabs). MBP cleavage was performed by incubation in cleavage buffer (50 mM Trizma-HCl ...
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bioRxiv - Genomics 2021Quote: ... the same amplicons were treated in vitro with the recombinant CpG methyltransferase M.SssI (NEB). Briefly ...
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bioRxiv - Immunology 2020Quote: ... All in vitro activity assays with recombinant GST-IdeZ or IdeZ (NEB Catalog #P0770S) (1ug per reaction ...
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bioRxiv - Neuroscience 2022Quote: Recombinant proteins were labeled with SNAP-Surface 549 dye (New England BioLabs, Cat# S9112S) for visualization ...
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bioRxiv - Developmental Biology 2020Quote: ... the Lox-Stop-Lox site was removed by exposure to recombinant Cre recombinase (NEB), using the NEB standard protocol ...
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bioRxiv - Plant Biology 2022Quote: ... For recombinant protein expression we used GW versions of pMAL-C2 (New England Biolabs) and pDEST-17 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... The recombinant proteins were affinity purified through GST tag binding to amylose resin (BioLabs) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... All S recombinants were constructed via gene fragment Assembly (New England Biolabs, catalog #: E2621S).
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bioRxiv - Neuroscience 2021Quote: The rat HA-tagged PRG-1 ORF construct was generated by Gibson Assembly Cloning from NEB (Ipswich, MA, #E5510S) of the Plppr4 ORF clone (NM_001001508.2 ...
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bioRxiv - Microbiology 2020Quote: ... The Flag-tagged spike gene was ligated to the linearized vector using the T4 DNA Ligase enzyme from NEB. The resulting plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... new SNAP-tagged histones were pulse-labeled for 30 min with 5 μM final SNAP-biotin (New England Biolabs) diluted 1:200 in 10% Duolink blocking buffer (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged ORF3c mutant Q5Y was prepared by site-directed mutagenesis using a commercial kit (New England Biolabs, E0554S).
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bioRxiv - Cancer Biology 2023Quote: ... Gαi2R179C and GαoAR243H mutants were constructed using pcDNA3.1 wild-type human ee-tagged-Gαi2- and ee-tagged-GαoA plasmids as template (cDNA Resource Center) and Q5 Site-directed mutagenesis kit (New England BioLabs). The lentiviral vector for tetracycline-inducible expression of the Gαi2R179C and GαoAR243H mutants was constructed by first cloning Gαi2R179C and GαoAR243H from pcDNA3.1 to pENTR vector (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of immunopurified GST-tagged WRN fragment was phosphorylated in vitro by Casein Kinase II (New England Biolabs) in the presence of 1X NEBuffer (50mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... All truncated or tagged versions of clones were generated using Q5 high-fidelity DNA polymerase (New England Biolabs #M0515) and NEB-Gibson Assembly (New England Biolabs #E2611S) ...
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bioRxiv - Biochemistry 2024Quote: ... 0,5 μM fluorescently tagged 3’ adapter (MultiplexDX) were ligated with T4 Rnl2(1–249)K227Q (M0351, New England Biolabs) overnight at 4°C and washed three times with PNK/ligation buffer ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 μM fluorescently tagged 3’ adapter (MultiplexDX) were ligated with T4 Rnl2(1–249)K227Q (M0351, New England Biolabs) overnight at 4°C and washed three times with PNK/ligation buffer ...
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bioRxiv - Evolutionary Biology 2020Quote: Purified DNA library (250 ng) was cut with the Hind III and Bam HI restriction enzymes (NEB) for 1h at 37 °C in a reaction mixture containing 1X of the NEB cut smart buffer ...
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bioRxiv - Cell Biology 2022Quote: ... pET17b-Kif5b(1-560)-GFP-His was transformed into BL-21[DE3]RIPL cells (New England Biolabs) until an optical density at 600 nm of 0.6-0.8 and expression was induced with 0.5 mM isopropyl-β-d-thiogalactoside (IPTG ...
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bioRxiv - Genomics 2020Quote: ... We put the mutagenized genes in a pIVEX vector via Gibson assembly (NEB Hi-Fi DNA assembly) using 125ng of gene DNA ...
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bioRxiv - Biochemistry 2021Quote: ... and C-terminal additions) were assembled with the pcDNA5 backbone using Hi-Fi DNA assembly (NEB; E2621S).
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bioRxiv - Plant Biology 2022Quote: ... His-TAD1 fusion proteins were purified according to the manufacturer’s protocol (New England Biolabs, Ipswich, MA, USA). The bait protein GST-WL1 was incubated with GST beads (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... the product was cloned into the pET1-5b N-terminal 6×His expression plasmid (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... The His-GST tags were cleaved by incubating the pooled fractions with TEV protease (New England Biolabs) at 4°C for 16h ...