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Citations for New England Biolabs :
1401 - 1450 of 2029 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... coli NEB 10-beta from New England Biolabs (NEB) was used for sensor assays ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 Star (DE3)pLysS (New England Biolabs, #C2527H) were grown in Luria-Bertani (LB ...
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bioRxiv - Immunology 2023Quote: ... Upon Escherichia coli BL21 (DE3) transformation (New England Biolabs), expressed proteins were isolated from inclusion bodies ...
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bioRxiv - Biochemistry 2023Quote: ... coli strains BL21(DE3) (New England Biolabs, ref. C2527I) were grown in LB medium containing kanamycin (50 µg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... coli SHuffle T7 cells (New England Biolabs, Ipswich, MA) in TB medium at 17 °C ...
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bioRxiv - Microbiology 2023Quote: ... coli strains following the chemical transformation protocol from NEB for functional characterization ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3) chemically competent cells (New England Biolabs) using standard protocols ...
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bioRxiv - Bioengineering 2023Quote: ... coli strains: NEB 5-alpha (NEB, C2987; not authenticated), BL21-AI (Thermo Fisher ...
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bioRxiv - Genomics 2023Quote: ... coli poly-A polymerase (New England Biolabs, Cat. # M0276S). The samples were incubated at 37°C for 10 mins ...
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bioRxiv - Synthetic Biology 2023Quote: ... Escherichia coli strains used were DH5α (NEB cat C2987H), E ...
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bioRxiv - Microbiology 2023Quote: ... coli BL21(DE3) cells (New England Biolabs Frankfurt, Germany). Starter cultures (5 mL ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli NEB10beta (New England Biolabs, Ipswich, MA, USA, #C3020K) were transformed by 4 µl of dialyzed bacmid assemblies according to the manufacturer instructions and plated on chloramphenicol (25 µg/ml)-containing LB-agar plates and incubated overnight ...
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bioRxiv - Cell Biology 2023Quote: ... coli BL21 (DE3) competent cells (New England Biolabs, C2527), which were incubated on Luria Bertani (LB ...
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bioRxiv - Biophysics 2023Quote: ... coli BL21(DE3) chemically competent cells (New England Biolabs). Cultures of 500 mL in LB broth were induced at OD 0.6 with 1mM IPTG at 18 °C overnight ...
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bioRxiv - Molecular Biology 2024Quote: Escherichia coli DH5α and ER1821 (New England Biolabs (NEB), USA ...
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bioRxiv - Molecular Biology 2024Quote: Escherichia coli DH5α and ER1821 (New England Biolabs (NEB), USA ...
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bioRxiv - Microbiology 2024Quote: ... coli DH5α (Cat. C2988J, New England Biolabs, Ipswich, MA) and stored at -80°C ...
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bioRxiv - Biochemistry 2024Quote: ... coli Shuffle T7 Express cells (C3029J, New England Biolabs) to aid disulfide bond formation ...
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bioRxiv - Microbiology 2023Quote: ... coli competent cells (New England Biolabs, Ipswich, MA, USA) ...
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bioRxiv - Biochemistry 2024Quote: ... coli NEB511 were purchased from New England Biolabs (NEB). Headspace vials were purchased from Chemglass Inc.
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bioRxiv - Biophysics 2024Quote: ... coli SHuffle T7 Express (New England Biolabs, Ipswich, MA) cells were transformed with 100 ng pET32-Trx-LqhαIT and ...
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bioRxiv - Biophysics 2024Quote: ... Escherichia coli BL21 (DE3) competent cells (New England Biolabs) were transformed with a pET28a vector containing the N-terminal six-histidine tagged human TG2 ...
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bioRxiv - Microbiology 2023Quote: ... coli competent cells (New England Biolabs, Ipswich, MA, USA).
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bioRxiv - Cell Biology 2021Quote: Myc- and DDK (FLAG)-tagged Hemopexin mRNA was transcribed from an AgeI (NEB) linearised plasmid using T7 RNA polymerase (Promega) ...
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bioRxiv - Plant Biology 2022Quote: ... Each MBP-tagged RAPTOR1B fragment was detected with anti-MBP antibodies (NEB, E8032S). For the in vitro kinase assay ...
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bioRxiv - Microbiology 2022Quote: ... and tagged for multiplexing via the NE Next Multiplex Oligos for Illumina (NEB).
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bioRxiv - Molecular Biology 2022Quote: ... The HA-tagged PTBP1 was inserted into pcDNA3 between the EcoRI (NEB, R3101S) and NotI (NEB ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The MBP tagged proteins were purified by using Amylose Resin (New England Biolabs) and Ultrafiltration tubes (Millipore ...
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bioRxiv - Genomics 2021Quote: ... The purified recombinant protein or commercial M.EcoGII (NEB, M0603S) was diluted with coating buffer (0.05 M NaHCO3 buffer ...
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bioRxiv - Biochemistry 2021Quote: ... This experiment was further repeated with recombinant Histones (NEB) (0.5µg ...
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bioRxiv - Genetics 2020Quote: ... MEFs were protein transfected with recombinant RNase H (NEB) using Project Reagent Transfection Kit (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: ... a combination of both recombinant RBPMS and BSA (NEB). Splicing reactions were incubated for the 3 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... 20pmol of recombinant histone H3.1-H4 tetramer (NEB, M2509S) were immediately added to the tube ...
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... Recombinant human Histone H2A (New England BioLabs, Catalog # M2502S) or H4 (New England BioLabs ...
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bioRxiv - Genetics 2019Quote: ... Hi-C DNA was amplified using Index Primers set 1 (NEB, E7335S). The Hi-C libraries were purified with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... and the fragment was assembled using Hi-Fi DNA Assembly Mix (NEB) into the donor vector pADN171XS7 ...
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bioRxiv - Microbiology 2021Quote: ... His-MBP-MirA were first isolated using amylose bead affinity chromatography (NEB) in conditions specified by the manufacturer ...
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bioRxiv - Plant Biology 2021Quote: ... The homology plasmids were assembled using HI-Fi DNA Assembly Mastermix (NEB) with four PCR-generated DNA fragments (see Table S2 for primers) ...
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bioRxiv - Molecular Biology 2019Quote: ... purified and then cleaved with Xho I and Bam HI (both NEB). After further purification ...
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bioRxiv - Microbiology 2021Quote: ... Then the His-tag was cleaved by incubation with TEV protease (NEB) for 18 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... and then ligated by Hi-Fi DNA assembly (New England Biolabs Inc.). Irgb6-WT and Irgb6-T95D were expressed and purified as described (Saijo-Hamano et ...
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bioRxiv - Biochemistry 2023Quote: ... was cloned into pUC19 by Gibson cloning (Hi-Fi cloning system, NEB) to replace the yeast sequence:
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bioRxiv - Biophysics 2021Quote: MukF Flag-tagged fragments were expressed from pET 21 plasmids in C3013I cells (NEB).The Flag-tagged MukE and MuF were at the C-terminus ...
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bioRxiv - Cell Biology 2021Quote: ... MBP-tagged fragments were purified by gravity flow through amylose resin (New England Biolabs) and step elution in maltose ...
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bioRxiv - Biochemistry 2023Quote: ... containing a His6-tagged Ulp1 gene was transformed into T7 Express competent cells (NEB) and grown in LB medium supplemented with ampicillin (100 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... MBP-tagged CCDC22 and CCDC93 proteins were purified using Amylose beads (New England Biolabs), mixed in approximately 1:1 stoichiometry ...
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bioRxiv - Bioengineering 2020Quote: ... coli DH10B strain (NEB #C3019 New England Biolabs, Ipswich, MA) using PureLink Quick Plasmid Miniprep Kit (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... for HlgA and in BL21 (DE3) Escherichia coli cells (NEB) for HlgB ...
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bioRxiv - Cell Biology 2020Quote: KCM competent bacteria (Escherichia coli strain K12 CJ236, NEB, E4141) were transformed with 3Cs multiplex template plasmid template by mixing 100 ng of DNA with 2 µl of 5x KCM buffer (0.5M KCl ...
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bioRxiv - Microbiology 2019Quote: ... coli strains NEB 5-alpha F’lq (New England Biolabs, US) and MC1061 with plasmid pKD46 which carries ARA-inducible Red recombination system24 ...