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Citations for New England Biolabs :
1551 - 1600 of 2029 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... PCR confirmation of endogenously tagged ATR cell lines was performed using NEB Taq DNA polymerase (NEB) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR was performed with Nextera-tagged primers under standard Phusion or Q5 Polymerase (New England Biolabs). PCR primers used in this study can be found in Supplementary Table 3 ...
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bioRxiv - Plant Biology 2022Quote: ... coli BL21 (DE3) and purified using Amylose Resin (MBP, New England Biolabs) according to the manufacturer’s introduction as well as recombinant Arabidopsis histone MBP-H31-57 ...
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bioRxiv - Microbiology 2019Quote: ... Bacillus subtilis 3610 (33) and Escherichia coli strains NEB10β (New England Biolabs) and WM3064 were cultured aerobically on LB agar or in LB broth ...
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bioRxiv - Genetics 2019Quote: ... coli strains NEB10®-beta (New England BioLabs Inc., Ipswich, MA, USA), and XL1-blue (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain T7 Express lysY/Iq (New England Biolabs, Ipswich, MA, USA) and purified by Ni- ...
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bioRxiv - Bioengineering 2021Quote: Escherichia coli strains DH5α [15] and ER2523 (New England Biolabs, Ltd., UK) were grown in Luria Bertani (LB ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21 T7 Express lysY/Iq cells (New England BioLabs, cat. # C3013). The bacterial cultures were grown at 37 °C to an optical density (OD600 ...
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bioRxiv - Biophysics 2021Quote: RecA protein from Escherichia coli was purchased from New England BioLabs (NEB), Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... coli for protein purification were derivatives of pMal-c5X (New England Biolabs), which carries an AmpR marker and uses an IPTG-inducible tac promoter to expresses recombinant proteins with a factor Xa cleavable maltose-binding protein (MBP ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were expressed in Escherichia coli T7 Express (New England Biolabs) and grown in Luria-Bertani medium ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cells were used (New England Biolabs GmbH, Germany,Art. No. C2987). This strain was grown in Luria-Bertani (LB ...
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bioRxiv - Plant Biology 2019Quote: ... coli BL21 and purified by Amylose Resin (New England Biolabs, Ipswich, USA). One µg of kinase protein was incubated in kinase buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli strain DH10-β cells (45 μL each; C3019I, New England Biolabs) thawed on ice for 10 minutes and mixed gently by tapping ...
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bioRxiv - Microbiology 2021Quote: All plasmid protection assays were done in Escherichia coli (strain: NEB Turbo). As described previously12 ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid was transformed into Escherichia coli expression strain BL2lλDE3 (New England Biolabs) for expression ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells were incubated at 37°C (30°C for NEB stable), 200 min-1 either in 5 ml (for a high copy ...
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bioRxiv - Biochemistry 2020Quote: Proteins have been expressed in Escherichia coli strain BL21 (DE3) (NEB # C2527). In brief ...
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bioRxiv - Microbiology 2020Quote: ... coli Top10 cells and purified by using a Monarch miniprep kit (NEB). A Ty tag DNA sequence was inserted at the 3’-end of the gat1 coding sequence using a Q5 site directed mutagenesis kit (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... transformed into NEB 5-alpha chemically competent Escherichia coli (New England BioLabs), and submitted for Sanger sequencing (Eurofins Genomics ...
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bioRxiv - Microbiology 2020Quote: Escherichia coli BL21 (DE3) pRIL and SHuffle T7 (New England Biolabs, USA) strains were routinely grown in Luria Bertani (LB) ...
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bioRxiv - Biophysics 2022Quote: ... TnsC clones were transformed into Escherichia coli BL21(DE3) competent cells (NEB), while TnsB and S15 clones were transformed into homemade T1 phage-resistant pRIPL BL21(DE3) ...
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bioRxiv - Molecular Biology 2023Quote: ... coli poly (A) polymerase (New England Biolabs® Inc., Ipswich, MA, USA), and the reaction mixture was incubated at 370 C for 30 minutes ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli sfGFP expression cassette via Q5 site-directed mutagenesis (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: The strain Escherichia coli NEB 5-alpha (New England Biolabs, NEB C2987H) was used as a cloning strain ...
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bioRxiv - Molecular Biology 2023Quote: ... coli competent cells (NEB 5-alpha, New England Biolabs, Ipswich, MA, USA) that were cultured and prepared using a GenElute HP Plasmid Midi kit (NA0200-1KT ...
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bioRxiv - Biophysics 2023Quote: ... coli σ70 (σ70) was overexpressed in T7 Express cells (New England Biolabs) as inclusion bodies from the pET-28a-σ70 overexpression plasmid39 by growing the cells at 37oC to an OD600 of ∼ 0.8 ...
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bioRxiv - Microbiology 2023Quote: The strain Escherichia coli NEB 5-alpha (New England Biolabs, NEB C2987H) was used as a cloning strain ...
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bioRxiv - Cell Biology 2023Quote: ... The amplified product was transformed in Escherichia coli NEB5α cells (NEB#C2987) to obtain colonies ...
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bioRxiv - Genomics 2023Quote: ... All constructs were polyadenylated using Escherichia coli poly(A) polymerase (NEB, M0276S) according to manufacturer’s instructions followed by a clean up using the RNA Clean & Concentrator-5 kit (Zymo Research ...
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bioRxiv - Biochemistry 2023Quote: ... coli strains Nico21 (DE3) pLysS or BL21-codon plus (New England BioLabs) were used for overexpression ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Poly-adenylation followed using Escherichia coli Poly(A) Polymerase (New England Biolabs) for 30 minutes at 37 °C and products were further cleaned with RNA clean & concentrator 5 (Zymo Research ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells were used (New England Biolabs GmbH, Germany, Art. No. C2987) for the cloning of certain plasmids ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli NGF-1 was amplified directly using Q5 PCR (New England Biolabs), using primers p117 and p118 (Supplementary Table 1 ...
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bioRxiv - Cell Biology 2024Quote: Plasmids were transformed in BL21 (DE3) competent Escherichia coli (New England Biolabs) or Rosetta (DE3 ...
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bioRxiv - Cell Biology 2022Quote: ... Afterwards 2 µg of recombinant human Histone H3.1 (New England BioLabs, M2503), 100 µM S-(5′-adenosyl)-L-methionine chloride dihydrochloride (SAM ...
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bioRxiv - Neuroscience 2019Quote: ... pCAV-FLExloxP-Flp was incubated with recombinant Cre recombinase (New England Biolabs) and then transformed into DHα bacteria ...
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bioRxiv - Neuroscience 2022Quote: ... the recombinant proteins were purified by amylose resin (#E8022S, New England Biolabs) according the manual instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... and 0.4 µL (16 U) of recombinant RNase inhibitor (New England Biolabs) in a final volume of 20 µL ...
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bioRxiv - Synthetic Biology 2019Quote: ... and 0.75 µL (30 U) of recombinant RNase inhibitor (New England Biolabs) in a final volume of 30 µL ...
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bioRxiv - Synthetic Biology 2021Quote: The recombinant CBX8-CD was expressed in BL21 (DE3) (New England Biolabs) Escherichia coli cells ...
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bioRxiv - Biophysics 2021Quote: ... Recombinant M-MuLV reverse transcriptase from the ProtoScript® II kit (NEB) was used to synthesize first strand cDNA from the annealed primer-MS2 RNA mix ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GFP-fusion proteins were purified from BL21(DE3) cells (NEB, # C2527H) using an N-terminal H6-tag ...
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bioRxiv - Biochemistry 2022Quote: The recombinant Lili-Mips were treated with PNGase F (New England Biolabs) to remove the N-linked oligosaccharides ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant vectors were generated using HiFi DNA Assembly Cloning Kit (NEB E5520). During cloning procedures ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant vectors were constructed using HiFi DNA Assembly Cloning Kit (NEB E5520). electroporation (device) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The cloning was performed with the NEBuilder Hi Fi DNA Assembly kit (New England Biolabs). The enzymes mentioned were purchased from New England Biolabs ...
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bioRxiv - Immunology 2023Quote: In situ Hi-C experiments were performed as previously described2 using restriction enzyme MboI (NEB) with minor modifications ...
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bioRxiv - Genomics 2023Quote: ... pooled oligonucleotides were amplified via PCR using NEBNext 2X Hi-Fi PCR Master Mix (NEB) and primers:
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bioRxiv - Microbiology 2023Quote: ... His/Strep-Tag was cleaved using enterokinase according to the protocol of the manufacturer (NEB) and GlnA3Mtwas immediately purified from the digestion mix by size-exclusion chromatography as directed by the resin manufacturer (GE-Healthcare).