Labshake search
Citations for New England Biolabs :
1501 - 1550 of 2029 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2020Quote: Escherichia coli NEB5α high efficiency competent cells were obtained from NEB for plasmid construction ...
-
bioRxiv - Microbiology 2020Quote: ... All cloned constructs were transformed into SHuffleT7 Competent Escherichia coli (NEB), and expression was induced with 1 mM IPTG (isopropyl-D-thiogalactopyranoside) ...
-
bioRxiv - Systems Biology 2021Quote: ... coli clones using the Monarch Plasmid Miniprep Kit (New England BioLabs).
-
bioRxiv - Molecular Biology 2020Quote: ... we used 5 units of Escherichia coli RNase HII (NEB, M0288L) in place of the C ...
-
bioRxiv - Microbiology 2019Quote: ... coli poly(A) polymerase (New England BioLabs, Beverly, MA, USA; M0276S).
-
bioRxiv - Biochemistry 2020Quote: ... coli BL21 T7 Express lysY/Iq cells (New England BioLabs, C3013). The cells were grown in LB medium containing 50 μg/ml kanamycin at 37°C until OD600 nm 0.5 ...
-
bioRxiv - Microbiology 2021Quote: ... coli using the NEBuilder HiFi DNA Assembly kit (New England Biolabs) following manufacturers’ instructions ...
-
bioRxiv - Bioengineering 2019Quote: Escherichia coli NEB5α high efficiency competent cells were purchased from NEB for plasmid construction ...
-
bioRxiv - Biochemistry 2020Quote: ... coli BL21 (DE3) were purchased as competent cells (New England Biolabs). E ...
-
bioRxiv - Cell Biology 2021Quote: ... coli (BL21/pLysS) carrying the expression constructs using amylose beads (NEB) and S-glutathione beads (GE Healthcare) ...
-
bioRxiv - Microbiology 2021Quote: ... coli poly-A polymerase (New England Biolabs, NEB, Ipswich, MA, USA) for 30 minutes at 37 °C ...
-
bioRxiv - Microbiology 2021Quote: ... coli poly-A polymerase (New England Biolabs, NEB, Ipswich, MA, USA) for 30 minutes at 37 °C ...
-
bioRxiv - Microbiology 2022Quote: ... coli host strain NEB 5-alpha F’ lq (New England Biolabs) was exclusively used to generate ...
-
bioRxiv - Molecular Biology 2023Quote: ... coli strain BL21 (DE3) and subsequently purified with amylose beads (NEB). The RNA affinity purification of mRNA 3’ processing complex was carried out essentially as described before (15) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Escherichia coli strains NEB Turbo and NEB Express (New England BioLabs) were used for cloning ...
-
bioRxiv - Biochemistry 2023Quote: ... coli DH5α and NEB10ß cells (New England Biolabs GmbH, Frankfurt, Germany) were used.
-
bioRxiv - Bioengineering 2023Quote: ... coli strains used in this study were DH5α (New England Biolabs) for cloning purposes ...
-
bioRxiv - Immunology 2023Quote: ... for transformation with competent Escherichia coli NEB5a bacteria (New England Biolabs) and colony selection ...
-
bioRxiv - Molecular Biology 2023Quote: ... and then transformed into 5-alpha competent Escherichia coli cells (NEB), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... All plasmids were transformed into Escherichia coli Stable3 competent cells (NEB). The identity of all plasmids was confirmed by Sanger Sequencing ...
-
bioRxiv - Bioengineering 2024Quote: ... coli strains used in this study were DH5α (New England Biolabs) for cloning ...
-
bioRxiv - Microbiology 2024Quote: ... coli B strain was dephosphorylated with 0.4 U/µL QuickCIP (NEB) in 1 x rCutSmart buffer (NEB ...
-
bioRxiv - Biophysics 2020Quote: Recombinant wild-type human histone H1.0 (New England Biolabs, cat. # M2501S) was used for experiments with fluorescently-labeled nucleosomes ...
-
bioRxiv - Bioengineering 2021Quote: Recombinant RfxCas13d proteins were expressed in E.coli NiCo21(DE3) (NEB C2529). Cells were grown in 1L of lysogeny (Luria-Bertrani ...
-
bioRxiv - Genetics 2019Quote: ... after the recombinant plasmids linearized with NotI Restriction Enzyme (NEB, USA), and then the capped mRNAs were purified by RNeasy Mini Kit (Qiagen ...
-
bioRxiv - Immunology 2020Quote: ... and dephosphorylated using recombinant shrimp alkaline phosphatase (New England BioLabs M0371L). Annealed and phosphorylated oligonucleotides were cloned into linearized and dephosphorylated BPK1520_puroR using T4 DNA Ligase (New England BioLabs M0202L ...
-
bioRxiv - Immunology 2021Quote: ... A similar dilution series of recombinant histone H3 (New England Biolabs) was prepared starting at 1 µg/ml protein ...
-
bioRxiv - Biochemistry 2021Quote: Recombinant human CHIP was expressed in BL21(DE3) (New England Biolabs) E ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 5 mM DTT with 1.5 µM recombinant H3 (New England Biolabs), vehicle or 500 nM recombinant enzyme ...
-
bioRxiv - Immunology 2021Quote: ... Recombinant proteins were induced in SHuffle Express strains (New England Biolabs) upon addition of 1mM IPTG in cultures containing kanamycin 50 μg/mL and incubated with agitation at 37 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... Recombinant bacmids were created by transformation into DH10Bac competent cells (NEB), screening on XGAL plates ...
-
bioRxiv - Biochemistry 2024Quote: ... Protease Inhibitors) with recombinant PKA kinase (2500 U/mg, NEB-P600S) and 1 mM ATP overnight at 30°C and 250 rpm ...
-
bioRxiv - Cancer Biology 2020Quote: ... and E-cadherin (24E10, Cell Signaling Technology, NEB, Frankfurt, Germany, #3195, 1:400) were used for immunohistochemistry (IHC ...
-
bioRxiv - Developmental Biology 2022Quote: ... The complete insert region was PCR amplified using Q5 Hi Fidelity DNA polymerase (NEB) and the amplicon sequenced at high coverage using Illumina MiSeq in the Complete Amplicon Next-Generation Sequencing service from the MGH CCIB DNA Core (Fig ...
-
bioRxiv - Microbiology 2021Quote: ... Reactions were conducted using NEB Phusion Hi Fi Polymerase (New England Biolabs, Ipswich, MA). Reactions were comprised of 4 µl 5X Phusion Buffer ...
-
bioRxiv - Microbiology 2021Quote: ... Reactions were conducted using NEB Phusion Hi Fi Polymerase (New England Biolabs, Ipswich, MA). Reactions were comprised of 4 µl 5X Phusion Buffer ...
-
bioRxiv - Cancer Biology 2021Quote: ... 40 μg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C to remove of biotin from non-ligated fragment ends ...
-
bioRxiv - Biochemistry 2020Quote: ... His-SNAP-EB3 proteins were then coupled to SNAP-Cell 647-SiR dye (NEB) by incubation at 37°C for 30 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... The expression vector pET302/NT-His was digested with EcoR1 restriction enzyme (NEB R0101S) and run on a 1.5 % agarose gel ...
-
bioRxiv - Molecular Biology 2019Quote: ... 40 µg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C ...
-
bioRxiv - Synthetic Biology 2019Quote: ... cloning was performed with the NEBuilder Hi Fi DNA Assembly kit (New England Biolabs). PCR reactions were performed with the Phusion High-Fidelity PCR Master Mix with HF buffer (New England Biolabs) ...
-
bioRxiv - Genetics 2019Quote: ... These were used in a PCR reaction with Q5 Hi-Fidelity polymerase (NEB, USA) in combination with the pLuc-GAL5.1 reporter construct previously described 8 as template to produce pLuc-GALΔEGR ...
-
bioRxiv - Genomics 2019Quote: ... 40 μg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... using the NEBuilder Hi Fi DNA Assembly Master Mix (New England Biolabs, Beverly, MA). For the production of lentiviral particles ...
-
bioRxiv - Biochemistry 2023Quote: ... The His-MBP-Cas12a plasmid was transformed into BL21(DE3) cells (New England Biolabs). A single colony was used to inoculate LB media supplemented with 50μg/ml Kanamycin for an overnight culture grown at 37°C ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... stained in HI buffer containing 5 μM SNAP-Surface Alexa 647 (New England Biolabs) for 15 minutes at room temperature ...
-
bioRxiv - Biochemistry 2023Quote: ... and K723G).49 His/MBP-BRAF NTs were created with standard Gibson Assembly (NEB) procedure in the pET28-MBP vector from the following primers ...
-
bioRxiv - Cell Biology 2021Quote: ... Snap tagged human D1DR constructs were labelled with Snap-cell 647 SiR (New England Biolabs, S9102S) as described previously (94).
-
bioRxiv - Cell Biology 2021Quote: ... MBP-tagged protein was captured by gravity flow affinity chromatography using amylose resin (New England Biolabs). Captured protein was washed with wash buffer (50mM Tris/HCl pH 7.6 ...
-
bioRxiv - Cell Biology 2022Quote: All overexpression constructs with the internally tagged actins were generated by Gibson assembly (New England Biolabs). Briefly ...