Labshake search
Citations for New England Biolabs :
1451 - 1500 of 2029 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... coli BL21 Codon-Plus competent cells (C2527, New England Biolabs) and grown at 37 °C in 2 % Luria Broth containing 30 mg/L kanamycin until OD600 reached 0.6 ...
-
bioRxiv - Neuroscience 2020Quote: ... coli BL21 Codon-Plus competent cells (C2527, New England Biolabs) and grown at 37 °C in 2 % Luria Broth containing 0.2 % glucose and 100 mg/L ampicillin until OD600 reached 0.3-0.37 ...
-
bioRxiv - Biochemistry 2020Quote: ... coli BL21 (DE3) cells (New England Biolabs, Ipswich, MA, USA) were transformed with the untagged SiRHP-expressing pBAD vector (Askenasy et al. ...
-
bioRxiv - Microbiology 2021Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 50,000 variants per duplicate library ...
-
bioRxiv - Biochemistry 2022Quote: ... coli BL21(DE3) (New England Biolabs, Frankfurt am Main, Germany) cells were transformed with the pET22b-BcnrdI plasmid ...
-
bioRxiv - Biochemistry 2022Quote: ... coli strain NEB 10-β competent cells (New England Biolabs) and plated on LB agar containing 50 μg/mL kanamycin ...
-
bioRxiv - Microbiology 2022Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 100,000 variants per duplicate library ...
-
bioRxiv - Cell Biology 2022Quote: ... coli BL21(DE3) cells (New England Biolabs, cat. no. C2527I). A starter culture grown in LB broth (Sigma ...
-
bioRxiv - Synthetic Biology 2021Quote: ... coli Poly(A) Polymerase (10 units; M0276S, New England Biolabs) with nuclease-free water (29 μL) ...
-
bioRxiv - Microbiology 2021Quote: ... coli BL21(DE3) cells (T7 Expression strain, New England Biolabs), expressed from a pET28b plasmid ...
-
bioRxiv - Microbiology 2021Quote: ... All assays were done in Escherichia coli (strain: NEB Turbo) following the same plasmid protection assay described previously ...
-
bioRxiv - Microbiology 2019Quote: ... All pCas9 constructs were transformed into Escherichia coli (NEB Turbo) by heat-shock and made electrocompetent as described (protocols.io ...
-
bioRxiv - Microbiology 2020Quote: ... coli cloning strains such as NEB-5a (New England Biolabs) or BH10c,48 with antibiotics added at the following concentrations ...
-
bioRxiv - Molecular Biology 2021Quote: ... coli Stbl competent cells (New England Biolabs, Ipswich, MA, USA), colonies were screened by PCR ...
-
bioRxiv - Microbiology 2021Quote: ... coli DH5-alpha cells following manufacturer’s protocol (New England Biolabs). Sanger sequencing was used to confirm plasmid construction ...
-
bioRxiv - Immunology 2022Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs #C3020K), and bottlenecked to ∼1 × 105 cfus (an average of >25 barcodes per single-mutant) ...
-
bioRxiv - Biochemistry 2022Quote: ... coli were transfected using the High-Efficiency Transformation Protocol (NEB). Single colonies were selected from carbenicillin LB plates and plated into 5 mL starter cultures ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... coli in 5 x 25 uL cell aliquots (NEB #C3020). Each cell aliquot was recovered in 1 mL outgrowth media at 37°C for 1 hr and then transferred into 100 mL of molten LB (1% tryptone ...
-
bioRxiv - Microbiology 2022Quote: Escherichia coli strain NEB Stable (New England Biolabs, Ipswich, MA), Pseudomonas aeruginosa strains PA01 and PA14 (generous gift from Dr ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Coli BL21(DE3) strain (purchased from New England Biolab, NEB), BL21Marioentte (purchased from Addgene ...
-
bioRxiv - Biochemistry 2023Quote: ... coli strain SHuffle T7 (New England Biolabs Inc., Massachusetts, USA) and plated on LB agar containing 30 μg/mL kanamycin and 2% glucose ...
-
bioRxiv - Bioengineering 2023Quote: ... coli MG1655 gDNA (cat no. T3010, New England Biolabs Inc.) via PCR ...
-
bioRxiv - Microbiology 2023Quote: ... Strains used were: Escherichia coli T7 Shuffle (New England Biolabs) to express Rv0365c ...
-
bioRxiv - Biochemistry 2023Quote: ... coli BL21 strain resistant to phage T1 (New England Biolabs) harboring the pRARE2 plasmid and plated onto LB+Agar plates supplemented with kanamycin (Kan ...
-
bioRxiv - Genetics 2024Quote: ... coli competent cells (New England Biolabs, MA, USA, Cat # C2987H) from the pDONR 221 entry vector to the pGW-HA.attB destination vector using Gateway cloning and the sequences were confirmed by Sanger sequencing ...
-
bioRxiv - Microbiology 2024Quote: ... Competent strains of Escherichia coli DH5-α (New England Biolabs), used for DNA cloning and plasmid propagation ...
-
bioRxiv - Microbiology 2024Quote: ... coli strain carrying the plasmid (Monarch Mini-Prep kit, NEB) and transformed it into electrocompetent K ...
-
bioRxiv - Bioengineering 2024Quote: ... grown in 5-alpha competent Escherichia coli (NEB, Ipswich, MA) and purified using the PureYield MidiPrep System (Promega ...
-
bioRxiv - Biophysics 2024Quote: ... and then transformed BL21(DE3) Escherichia coli (New England BioLabs) with this plasmid ...
-
bioRxiv - Immunology 2021Quote: Purified recombinant CV3-25 IgG was mixed with LysC (NEB) at a ratio of 1μg LysC per 10mg of IgG and incubated at 37°C for 18h with nutation ...
-
bioRxiv - Molecular Biology 2022Quote: ... recombinant kinases (2500 U/mg protein for PKA (NEB-P600S), 500:1 Tau:kinase for Gsk3ß (BPS-40007) ...
-
bioRxiv - Cell Biology 2022Quote: ... purified recombinant PKA proteins (2,500 units/ml, New England Biolabs) along with 100 μM cAMP ...
-
bioRxiv - Biochemistry 2020Quote: 1 μg of human recombinant histone H4 (BioLabs, Cat# M2504S) was incubated with 50 μM n-butyryl- or isobutyryl-CoA and 0.2 μM of HAT1 at 30 °C for 1 hour ...
-
bioRxiv - Developmental Biology 2020Quote: ... Kinase assays were performed using recombinant murine c-Abl (NEB). The amounts of GST-fusion protein to add to the reaction were calibrated using western blots of the purification products with mouse anti-NICD (1:1000 ...
-
bioRxiv - Biochemistry 2021Quote: ... Recombinant hACE2 protein was digested using EndoH (New England Biolabs) and thrombin (Sigma-Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... recombinant proteins were treated with PNGase F (New England Biolabs) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... PCR-amplified recombinant DNA was treated with DpnI (NEB Biolabs) for 3 h ...
-
bioRxiv - Cell Biology 2022Quote: ... PCR-amplified recombinant DNA was treated with DpnI (NEB Biolabs) for 3 h ...
-
bioRxiv - Genomics 2021Quote: ... 25 μL NEBNext Hi-Fi 2x PCR Master Mix (NEB (Ipswich, MA, USA) M0541S) ...
-
bioRxiv - Microbiology 2020Quote: ... and ligation with T4 ligase (Bioconcept) or by Hi-Fi Gibson assembly (NEB). PCR were performed using Phusion polymerase (Life Technologies ...
-
bioRxiv - Cell Biology 2021Quote: His-SpCas9-GFP was expressed in and purified from BL21 (DE3, NEB, C2527H) bacteria as previously described 47 ...
-
bioRxiv - Molecular Biology 2023Quote: Hi-C libraries were treated with 3 U of T4 DNA polymerase (NEB) in the presence of 0.1 mM dGTP (NEB ...
-
bioRxiv - Microbiology 2024Quote: ... and joined into the linearized vector by Hi-Fi Gibson DNA Assembly (NEB) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: GST or GST-tagged SH3 domains were expressed in BL21 (DE3) E.coli (New England Biolabs). Cells were lysed by sonication in presence of lysozyme (Affymetrix) ...
-
bioRxiv - Biochemistry 2021Quote: Pulldowns of MBP-tagged fusion proteins were performed using an amylose resin (New England Biolabs). Appropriate amounts of (see Fig ...
-
bioRxiv - Genetics 2019Quote: ... Amplicons were tagged using NEB Next Ultra II DNA Library Prep Kit for Illumina (NEB) with 5 thermal cycles for amplification and subjected to paired-end sequencing on a MiSeq platform using MiSeq Reagent Nano Kit v2 ...
-
bioRxiv - Molecular Biology 2019Quote: ... coli strains BL21-CodonPlus-RP or Lemo21(DE3, New England Biolabs). Bacterial lysates were prepared as previously described(48 ...
-
bioRxiv - Molecular Biology 2021Quote: ... coli NEB Stbl competent cells (New England Biolabs, Ipswich, MA, USA) and sequenced with U6 promoter primer listed in Supplementary Table S2.
-
bioRxiv - Molecular Biology 2021Quote: ... Escherichia coli competent cells NEB 5-alpha (C2987, New England Biolabs), NEB Stable (C3040 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... coli K-12 DH10B (Neb 10-beta, NEB catalogue no. C3019H), E ...