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Citations for New England Biolabs :
1801 - 1850 of 2029 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The point mutants were subsequently cloned in the pNZopuAHis plasmid (C-terminal 6-HIS-tag) using EcoRV-AlnwnI (NEB) for the substrate-binding domain mutants and βamHI-AlwnI (NEB ...
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bioRxiv - Biophysics 2020Quote: ... with TEV-cleavable 6x-His tag at N-terminal of the gene cloned between NheI (New England Biolabs Inc.) and HindIII (New England Biolabs Inc. ...
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bioRxiv - Cell Biology 2021Quote: ... Par-3 PDZ1-APM and PDZ1-APMΔPDZ2 were first his-purified (described above) prior to incubation with amylose resin (NEB). Amylose-bound Par-3 was then washed and resuspended in binding buffer as described for GST proteins.
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bioRxiv - Genomics 2021Quote: ... the Hi-C library was amplified for about 10 cycles of PCR with the Q5 master mix (NEB, M0492L), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 404–561 with a C terminal His tag added was cloned into the pMAL-c5x vector (New England Biolabs), and were transformed in E ...
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bioRxiv - Molecular Biology 2023Quote: The following M35 derivative constructs were derived from pcDNA3.1 TOPO M35-V5/His using the Q5 site-directed mutagenesis kit (#E0554, New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Near full-length 16S rRNA genes were amplified by PCR using Phusion Hi-Fidelity DNA Polymerase (New England BioLabs) and a bacteria-specific primer set ...
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bioRxiv - Neuroscience 2023Quote: ... These two amplicons were incubated with SfoI digested pET23a-His-TEV plasmid in a HiFi Assembly reaction (NEB, E2621S) to generate a plasmid where HaloTag and SpyCatcher3 is linked with a GSGESGSG linker sequence.
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bioRxiv - Microbiology 2023Quote: ... Gibson assemblies were completed using a 2x HI-FI Assembly master mix following the manufacturer’s protocol (New England Biolabs). PCR clean-up was done using the GeneJET PCR Purification Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: Hi-C single-index library preparation of MCF7 cells was performed as previously described using MboI (New England Biolabs) restriction enzyme (30).
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bioRxiv - Biophysics 2024Quote: ... pCAGEN_6×His-DNAJA2-HA was created from PCR of the ORF from pColdI_6×His-DNAJA2 and insertion into EcoRI-digested pCAGEN plasmids using NEBuilder HiFi DNA Assembly Master Mix (NEB). This plasmid was then used to produce pCAGEN_3xFLAG-DNAJA2 in a similar manner ...
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bioRxiv - Microbiology 2021Quote: ... the plasmid digestion was supplemented with 1.5 units of recombinant shrimp alkaline phosphatase (rSAP; NEB #M0371S). Digested insert and vector were purified and ligated together at room temperature for 30 min using T4 DNA ligase (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... after which 2 µL of Recombinant PNGaseF (Glycerol-free) (New England Biolabs, Ipswich, MA; catalog # P0709L) was added to each sample ...
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bioRxiv - Molecular Biology 2019Quote: ... These fragments were dephosphorylated with 30 units of recombinant shrimp alkaline phosphatase (M0371, NEW ENGLAND BIOLABS) for 1 hour at 37 °C with RNasin Plus (N2611 ...
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bioRxiv - Microbiology 2019Quote: ... 10% cRBCs in PBS were pre-treated with recombinant neuraminidase from Clostridium perfringens (New England Biolabs) for 1.5 hour at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant SYNJ2BP (0.5 µg) was dephosphorylated using 1 µl of calf intestinal alkaline phosphatase (CIP) (NEB) in 1x CutSmart Buffer in a total volume of 10 µl ...
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bioRxiv - Molecular Biology 2023Quote: ... the enzymatic activity of the commerically availablale recombinant vaccinia virus methyltransferase mRNA 2’O-methyltransferase (NEB), abbreviated hereagter as VMTR1 ...
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2021Quote: ... The E PBM C-terminal mutation (DVLL>GGGG) was performed using the Q5 site-directed mutagenesis kit (NEB). The sequences corresponding to the PDZ domains were cloned into the pCMV GFP vector using EcoRI and XhoI sites.
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bioRxiv - Biochemistry 2020Quote: ... pET-SUMO and pMALX-E were digested with Nde1/BamHI or Nhe1/ HindIII (New England Biolabs, Frankfurt, Germany), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... E-Syt3ΔC2C was produced by introducing a 2021_2257 deletion using the Q5 site-directed mutagenesis kit (NEN Biolabs). The deletion created a reading frameshift and a premature stop that removed the C2C domain and created a 707 aa long protein with a 35 aa C-neoextension ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic sequence of isolated clones at the target locus was further confirmed by PCR cloning (E#1202S, NEB) and Sanger sequencing (Australian Genome Research Facility ...
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bioRxiv - Microbiology 2023Quote: ... E protein glycosylation status was determined by treating RVP lysates with PNGase F (Cat#P0704S, New England Biolabs) for 3 hours at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Flag/V5-tagged TCF7L2 and Flag-tagged FOXQ1 truncation constructs were generated by restriction cloning using the high fidelity Q5 polymerase (New England Biolabs, Ipswich, US). For cloning of FOXQ1 truncation constructs ...
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bioRxiv - Biochemistry 2022Quote: ... and the impurities that were tagged by Chitin Binding Domain were extracted on a gravity-flow column using 5 mL Chitin resin (New England BioLabs, Evry, France). The target proteins were purified with HisTrap HP columns (GE Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: Amplicons of tagged-Cdep were isolated by gel extraction after PCR amplification using Phusion high fidelity polymerase (New England Biolabs, catalog #E0553L). The amplicons were then cloned into the pJFRC7 5x UAS vector (ref ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins (except from cells expressing eGFP and cytoplasmically tagged EGFR) were further treated with 250 U of PNGase F (NEB, Cat# P0704) for 1 hour at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... the constructed plasmids were transformed by heat shock into chemically competent Top10 Escherichia coli (New England BioLabs). Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1 ...
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bioRxiv - Bioengineering 2019Quote: ... Chemically-competent Escherichia coli DH10B cells were used for all routine cloning (New England Biolabs, Ipwich, MA). The E ...
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bioRxiv - Cancer Biology 2022Quote: ... was amplified using NEB Stable competent Escherichia coli (high efficiency) (C3040H, NEW ENGLAND BioLabs, Ipswich, MA, USA). The full-length ALKBH5 sequence (NM_017758) ...
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bioRxiv - Neuroscience 2019Quote: ... and used to transform NEB 5-α competent Escherichia coli cells (New England Biolabs, Whitby, ON, Canada). After screening plasmid constructs for directionality using T7 promoter oligonucleotide and gene-specific primers (Table S1) ...
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bioRxiv - Immunology 2021Quote: ... The assembled plasmid pCO2 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency) (NEB) according to manufacturer’s instructions (see Supplementary Figure 1 for plasmid maps) ...
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bioRxiv - Immunology 2022Quote: ... coli (C3019H/C3019I) was transformed with a plasmid DNA using High Efficiency Transformation Protocol (New England Biolabs). After picking a single colony ...
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bioRxiv - Microbiology 2023Quote: ... coli plasmid DNA were amplified by PCR with Phusion High-Fidelity DNA Polymerase (New England Biolabs, NEB) using primers as indicated in Table S1 ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′Iq Competent Escherichia coli (High Efficiency) (NEB, Cat# C2992H), and isolated with PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Bioengineering 2023Quote: ... The sequence-confirmed plasmid was transformed into Escherichia coli strain BL21(DE3) chemically competent cells (NEB C2527) according to NEB’s transformation protocol and plated on LB plates containing 100 µg/ml carbenicillin.
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bioRxiv - Bioengineering 2022Quote: ... The cloned plasmids were sequence verified using Sanger sequencing and transformed into T7-Express Escherichia coli (NEB) via heat-shock.
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bioRxiv - Microbiology 2023Quote: ... coli plasmid DNA were amplified by PCR with Phusion High-Fidelity DNA Polymerase (New England Biolabs, NEB) using primers as indicated in Table S1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were amplified for 12 PCR cycles with unique dual index primers using the NEBNext Hi-Fi 2X PCR Master Mix (New England Biolabs). Amplified libraries were purified using a 1.2X ratio of Axygen magnetic beads (Corning Inc ...
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bioRxiv - Molecular Biology 2019Quote: ... where C-terminal Myc-DDK tag was replaced by HIS-V5 tag using a NEBuilder HiFi DNA assembly kit (New England Biolabs). SHIP2 deletion in 293T cells was carried out by CRISPR/Cas9 technology (Ran et al ...
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bioRxiv - Bioengineering 2019Quote: ... inserting a N-terminal TEV cleavage tag and cloned into a pET28a backbone in front of the His-tag using the NEBuilder HiFi DNA assembly method (NEB).
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bioRxiv - Biochemistry 2019Quote: ... fused to a His-ZZ-TEV tag on the amino-terminus and a carboxy-terminal SNAPf tag (New England Biolabs)) and the pDyn2 plasmid (the pIDC expression vector with codon optimized DYNC1I2 ...
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bioRxiv - Genetics 2021Quote: ... FAN1 variants were introduced in the pFASTBAC1-GST-FAN1-FLAG-His plasmid by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Confirmed by DNA sequencing ...
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bioRxiv - Biochemistry 2020Quote: ... Vectors containing cohesin trimers were generated by combining pACEbac1 SMC1-His SMC3 with pIDC SCC1-2xStrepII by a Cre recombinase reaction (New England Biolabs). The vector for the Scc2/4 expression was also created by combining the pACEbac1 SCC2-2xStrepII with pIDC His-SCC4 using Cre recombinase.
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bioRxiv - Immunology 2021Quote: ... Restriction digest of pMK-RQ plasmid DNA containing EtIMP1 was performed using Bam HI and Not I restriction enzymes (New England Biolabs). Restriction digest of pYD1-EtAMA1Cit plasmid was performed with the same restriction enzymes to remove the EtAMA1 insert but retain the citrine tag ...
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bioRxiv - Microbiology 2021Quote: ... which were incubated with 1 μg/ml full-length S protein with His tag that had been pretreated with or without FXa (P8010L, NEB) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Tagmented DNA was amplified with 12 cycles of PCR using the NEBNext Hi-Fi 2X PCR Master Mix (NEB M0541) and unique dual index primers ...
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bioRxiv - Cell Biology 2021Quote: ... coding sequence was amplified from Arabidopsis cDNA and assembled into a pGreen0179-35S-spFLA11-His-YFP vector using NEBuilder HiFi DNA Assembly kit (NEW ENGLAND Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... open reading frames were cloned into a linearized pET28b vector with BamH1 and Xho1 in frame with a N- terminal 6x-His tag using Gibson HiFi assembly mix (NEB), 10 μL total reaction volume with 2 μL of linearized vector ...
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bioRxiv - Biochemistry 2022Quote: ... Constructs HsKHC-MDC and HsKHC-MDCL2-CaKip3 contained a cleavable C-terminal TEV-SNAP-6X-His tag (SNAP-tag from NEB). DARPin-D2 was ordered as a codon-optimized gene product from IDT and cloned into pET16b using Gibson Assembly ...