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Citations for New England Biolabs :
1551 - 1600 of 2232 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid transformation was achieved by using the heat shock method (42°C, 47s) in DH5α competent cells (NEB, C2987H), then purified with QIAGEN Plasmid Plus Midi Kit (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... and cloned into pTM1 (a pDONR/Zeo based plasmid) downstream of the ACT1 promoter using Gibson cloning kit (NEB), and then introduced into pDG33 using Gateway cloning (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Gαi2R179C and GαoAR243H mutants were constructed using pcDNA3.1 wild-type human ee-tagged-Gαi2- and ee-tagged-GαoA plasmids as template (cDNA Resource Center) and Q5 Site-directed mutagenesis kit (New England BioLabs). The lentiviral vector for tetracycline-inducible expression of the Gαi2R179C and GαoAR243H mutants was constructed by first cloning Gαi2R179C and GαoAR243H from pcDNA3.1 to pENTR vector (Thermofisher Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmids were constructed using the NEBuilder HiFi DNA assembly strategy of New England Biolabs (NEB, Frankfurt am Main, Germany)55 ...
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bioRxiv - Microbiology 2023Quote: ... The PCR fragments were inserted into the plasmid pG+host9 digested with PstI (New England Biolabs, Ipswich, Massachusetts, USA) by Gibson Assembly (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Construction of the PRP18/pUG35 and PRP18/YEp24 base plasmids was accomplished using Gibson assembly43 (New England Biolabs #E2611). Unless otherwise indicated in the figure legends ...
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bioRxiv - Cell Biology 2023Quote: ... Ligation of the plasmid was carried out via Gibson assembly as per manufacturer’s instructions (New England Biolabs; catalog # M5520AA2) and transfected into NEB5alpha bacteria ...
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bioRxiv - Developmental Biology 2023Quote: ... The reporter HLC plasmid (20ng μl-1) was digested by 0.5 U I-Sce enzyme in its adequate digestion buffer (NEB) for 30’ at 37°C prior to injections ...
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bioRxiv - Biochemistry 2023Quote: ... In a 50 μL reaction 1 μg of backbone plasmid (starting_eGPARK2iM) was digested at 37 °C for 1 hour with MluI-HF and EcoRI-HF (New England Biolabs), then heat-inactivated at 65 °C for 20 minutes ...
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bioRxiv - Microbiology 2023Quote: ... nLuc plasmids were linearized using SpeI and subjected to in-vitro transcription using HiScribe T7 kit (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... to generate the synthetic enhancer eGFP plasmid reporter (pTol2-synthetic enhancer-E1b-eGFP-Tol2) using HiFi DNA Assembly following manufacturer’s instructions (New England Biolabs). Reporter plasmid sequences were verified by Sanger sequencing ...
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bioRxiv - Cancer Biology 2023Quote: Plasmids containing the “circularization cassettes” were generated by using NEBuilder HiFi DNA Assembly Cloning Kit (#E5520S; New England BioLabs) and KLD Enzyme Mix (#M0554S ...
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bioRxiv - Biophysics 2023Quote: To construct the circular DNA for AFM experiments we used a commercially available pGGA plasmid backbone (New England Biolabs). We linearized the plasmid using MT032 and MT033 primers (Table S2) ...
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bioRxiv - Microbiology 2023Quote: ... These oligonucleotides were annealed together and the insert was cloned into pRH2521 plasmid using the BbsI restriction enzyme (NEB) and E ...
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bioRxiv - Immunology 2023Quote: ... and assembled into SalI linearized LentiGuide-GBP-Chr3 sgRNA3 plasmid using HiFi DNA Assembly Kit (New England Biolabs, E5520). Primers used were listed in Supplementary Table S3.
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bioRxiv - Cell Biology 2023Quote: ... Ligation of the plasmid was carried out via Gibson assembly as per manufacturer’s instructions (New England Biolabs; catalog # M5520AA2) and transfected into NEB5alpha bacteria ...
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bioRxiv - Cell Biology 2023Quote: ... Ligation of the plasmid was carried out via Gibson assembly as per manufacturer’s instructions (New England Biolabs; catalog # M5520AA2) and transfected into NEB5alpha bacteria ...
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bioRxiv - Molecular Biology 2023Quote: Preparative PCR for plasmid construction were performed with Q5 High-Fidelity DNA Poly-merase (New England Biolabs cat. #M0491S) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... digested plasmids and amplicons were ligated using the T4 DNA Ligase according to the manufacturer’s instructions (New England Biolabs). For Gibson Assembly ...
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bioRxiv - Cancer Biology 2023Quote: ... then ligated into a PB-CRISPRia expression plasmid linearized with BstXI and BlpI (New England Biolabs, Cat. #R0113L, #R0585L). Chemically competent Stable E ...
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bioRxiv - Biochemistry 2022Quote: ... the 6XHis-GB1-Aurora B (59-361 aa) and IN-box (790-844 aa; IN-boxΔC) coding regions were introduced in the pTXB1 plasmid (NEB) by in-fusion cloning using the Nde1 and Sap1 cloning site ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid inserts were amplified by PCR using the Q5® Hot-Start High-Fidelity 2X Master Mix (NEB). Site-directed mutagenesis was performed using the Q5® Site-directed mutagenesis kit (NEB ...
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bioRxiv - Biophysics 2023Quote: ... coli were introduced into the plasmid pRSET-A-FRET (Boersma et al, 2015) via Gibson assembly (New England Biolabs), so the encoded membrane anchor substituted the flexible linker between the mCerulean and mCitrine ...
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bioRxiv - Microbiology 2023Quote: The pGEX-6P-PrkA1-338 plasmid (Table 2) was constructed by a two-part ligation (NEB Quick Ligation kit) of BamHI- and NotI-linearized pGEX-6P and PCR-generated prkA1-338 amplified with primers AS21 and AS81 (Table 3 ...
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bioRxiv - Cell Biology 2023Quote: For protein synthesis the verified plasmid was transformed into BL21 competent cells (C2530, New England BioLabs, Ipswich, MA, USA). Cells were grown shaking at 37°C in 2XYT with appropriate selection to an OD600 of 0.6 and then transferred to 18°C ...
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bioRxiv - Microbiology 2023Quote: ... The complementation plasmid was constructed by Gibson assembly using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). In brief ...
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bioRxiv - Neuroscience 2023Quote: ... These two amplicons were incubated with SfoI digested pET23a-His-TEV plasmid in a HiFi Assembly reaction (NEB, E2621S) to generate a plasmid where HaloTag and SpyCatcher3 is linked with a GSGESGSG linker sequence.
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bioRxiv - Neuroscience 2023Quote: ... was used to replace the CMV promoter in pAAV.U6.shRLuc.CMV.ZsGreen.SV40 (Penn Core Vector) plasmid by excising out the CMV promoter using the EcoRI and NHE-I (NEB #R3131) cut sites ...
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bioRxiv - Cell Biology 2023Quote: ... The amplified HA-E2F2 fragment was assembled into the linearized FUW-tetO plasmid by Gibson Assembly (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... All expression plasmids were generated using standard cloning procedures and the NEBuilder HiFi DNA assembly system (New England Biolabs). All PCR amplified sequences were verified by DNA sequencing ...
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bioRxiv - Cell Biology 2023Quote: The resulting plasmids were mini-prepped and then digested with PmeI and SapI (R0560, R0569, NewEngland BioLabs, Waltham, MA) to generate a 6.01kb fragment containing the Whi3 coding sequence and 5’ UTR ...
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bioRxiv - Biochemistry 2023Quote: ... Both the δ insert and pET28 plasmid were digested with the restriction enzymes XbaI and BamHI (New England Biolabs) and then purified by gel extraction using the QIAquick Gel Extraction kit (Qiagen) ...
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bioRxiv - Biochemistry 2023Quote: ... were generated using the vPRC1.4 recombined plasmid (containing all three expression cassettes) as template and using Phusion polymerase (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: A 10 µL reaction containing 200 ng of pTrc200HA plasmid DNA and 1× CutSmart Buffer (New England BioLabs, USA) with partially purified V2c or its variants normalized by the major V2c protein band was incubated at 37°C for 1 hour ...
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bioRxiv - Systems Biology 2023Quote: ... originating from plasmid pGD009 20 was ligated into the backbone using NEB T4 Ligase (New England Biolabs, Ipswitch, MA) according to the manufacturers protocol using 29 fmol of backbone and 87 fmol of insert ...
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bioRxiv - Genetics 2024Quote: ... DNA template was prepared by cloning into a pUC19 backbone and plasmid was linearized using SapI restriction enzyme (NEB) and purified using DNA Clean & Concentrate (Zymogen) ...
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bioRxiv - Biochemistry 2023Quote: ... using the plasmid pSP73-JY0 as template (55) followed by digestion with the restriction enzyme MluI (New England Biolabs). Labelled handles were ligated with the central part with T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... and TurboID from Dr. Alice Ting’s plasmid (Tess C. Branon et al., 2018) via PCR using Q5 high-fidelity (M0492, NEB). Next ...
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bioRxiv - Synthetic Biology 2024Quote: The plasmid DNA was subjected to double digestion using KpnI and XbaI enzymes (New England Biolabs, Ipswich, MA, USA), followed by purification with the Wizard SV Gel and PCR Clean-up System (Promega Corporation ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA with similar profiles was additionally differentiated by digestion with the BamHI and HindIII restriction enzymes (NEB, USA). The digestion was performed according to the manufacturer’s recommendation ...
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bioRxiv - Developmental Biology 2023Quote: ... mixed in a ratio 1:3 (insert:backbone plasmid) and ligated with T4 ligase according to manufacturer’s instructions (Quick Ligation kit, NEB M2200). The ligation reaction was transformed into competent TOP-10 bacteria and plated on agarose plates with Ampicillin ...
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bioRxiv - Biophysics 2023Quote: ... PhoCl-2c and TMEM115 were cloned into the pTREtight2-vector plasmid using NEBuilder HiFi DNA Assembly (New England Biolabs). The FLAG-BK-YFP fragment was amplified from FLAG-BK667YFP plasmid27 ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid pEC095 was digested with BtsBI and the 5’ overhanging DNA ends were filled in with T4 Polymerase (NEB). A kanamycin resistance marker ...
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bioRxiv - Molecular Biology 2024Quote: ... and assembled with HindIII-digested pUC19mPheS plasmid using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs Inc.). The resulting plasmid was then transformed into E.coli DH10b strain for isolation via QIAprep Plasmid Mini-Prep Kit (Qiagen) ...
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bioRxiv - Genomics 2024Quote: ... Plasmid pools were digested with SphI-HF and SpeI-HF and treated with Antarctic phosphatase or Quick CIP (NEB), then ligated to reporter gene inserts in multiple reactions (NEB T4 ligase) ...
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bioRxiv - Biophysics 2024Quote: ... pCAGEN_6×His-DNAJA2-HA was created from PCR of the ORF from pColdI_6×His-DNAJA2 and insertion into EcoRI-digested pCAGEN plasmids using NEBuilder HiFi DNA Assembly Master Mix (NEB). This plasmid was then used to produce pCAGEN_3xFLAG-DNAJA2 in a similar manner ...
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bioRxiv - Synthetic Biology 2021Quote: ... and +1 sites of the promoters controlling transcription of both RNAs were used to amplify the standard pUC19 plasmid (NEB). After PCR amplification samples were digested with DpnI (NEB ...
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bioRxiv - Biophysics 2021Quote: The DNA substrate used in the optical trapping assay was a ~3400-bp PCR-amplified section of plasmid PBR322 (New England Biolabs). The forward primer (5’-/btn/-ACA GCA TCG CCA GTC ACT AT-3’ ...
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bioRxiv - Biophysics 2021Quote: The assembly of GRB2 SH3 and PSD95 PDZ mutant libraries in both assay plasmids were done overnight by temperature-cycle ligation using T4 ligase (New England Biolabs) according to the manufacturer's protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The sgRNA expression plasmids were cloned by insert annealed oligos into the pU6-sgRNA vector that was digested by Bbs I (NEB). shRNA for LacZ (negative control ...