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Citations for New England Biolabs :
1451 - 1500 of 2232 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... All PCR amplifications for plasmid and vector constructions were performed using Q5 polymerase (New England Biolabs, Ipswich, MA). Most PCR reactions were performed using the following conditions ...
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bioRxiv - Molecular Biology 2024Quote: ... All PCR amplification for plasmid and vector constructions were performed using Q5 polymerase (New England Biolabs, Ipswich, MA). Most PCR reactions were performed using the following conditions ...
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bioRxiv - Immunology 2024Quote: ... we found cloning was most efficient when we digested the library and plasmid with BsmBI-v2 (NEB, #R0739L) (but not the barcode fragment due to the quantity ...
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bioRxiv - Developmental Biology 2023Quote: ... The plasmids harboring the desired insertions (UPR TF coding sequences) were linearized by incubation with restriction enzymes (NEB). Linearized plasmids were subjected to invitro transcription using a SP6 Transcription Kit (Invitrogen AM1340) ...
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bioRxiv - Biochemistry 2024Quote: Plasmid constructs were cloned by Gibson assembly(51) using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Codon-optimised gene fragments for wild-type MxEnc and the triple mutants 3×His-MxEnc ...
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bioRxiv - Evolutionary Biology 2024Quote: ... To confirm integration of the ampicillin marker plasmid were digested with ScaI and XbaI (NEB R3122L and R0145L) and verified using gel electrophoresis ...
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bioRxiv - Bioengineering 2024Quote: ... Guide RNA spacers were cloned into rcRNA vectors by digesting plasmids with Esp3i restriction enzyme (New England Biolabs) for 1 hour at 37°C according to manufacturer’s directions ...
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bioRxiv - Bioengineering 2024Quote: ... The pTA-Mob plasmid (gift from Thomas Hamilton and David Edgell, Western University) was linearized using AvrII (NEB) and combined with the amplified fragment from pFAST-PETase-cis using a modified NEB Hifi Assembly protocol with a two-hour assembly step at 50 °C ...
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bioRxiv - Biochemistry 2024Quote: ... plasmid libraries were first double-digested with PmlI and MfeI-HF restriction enzymes (New England Biolabs, Ipswich, MA), then purified using a Zymo DNA Clean & Concentrator-5 kit (Zymo Research ...
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bioRxiv - Genetics 2021Quote: ... The synthetic oligo was cloned into the plasmid backbone by using the Gibson assembly kit (NEB, Gibson Assembly®) and the ligation reaction was carried out for 1 hour at 50 °C ...
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bioRxiv - Microbiology 2019Quote: ... The original plasmid template was then digested using the restriction enzyme DpnI in Cutsmart buffer (1X) (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Polymerase chain reaction (PCR) products and plasmids were purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and Sanger sequencing was performed by Eurofins Genomics ...
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bioRxiv - Microbiology 2022Quote: ... we first generated two plasmids encoding guide RNAs targeting either MMCAP2_0582 or MMCAP2_0575 by modification of the base plasmid p426-SNR52p-gRNA.Y-SUP4t (25) using the Q5 Site-Directed Mutagenesis kit (NEB). In addition ...
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bioRxiv - Neuroscience 2021Quote: RNAs were in vitro transcribed from PspOMI-linearized pcDNA3.1(+) reporter plasmids using HiScribe T7 High Yield RNA Synthesis Kit (NEB) with 3’-O-Me-m7GpppG anti-reverse cap analog (ARCA ...
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bioRxiv - Biophysics 2022Quote: ... The amplified plasmid containing the mutated p66 was double digested with NdeI and XhoI restriction enzymes (New England Biolabs), gel purified and cloned into MCS-2 of the pETDuet-1 vector pre-digested with the same set of restriction enzymes and carrying mutated p51 domain in MCS-1 ...
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bioRxiv - Microbiology 2020Quote: ... IVT gRNA was produced from SFV infectious clone plasmid and treated with 10 U of DNase1 (RNase-Free; NEB) for 30 min at 37°C before purification with the RNeasy MiniElute Cleanup kit (Qiagen ...
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bioRxiv - Developmental Biology 2019Quote: ... and the Tol2-5xUAS-TATA:M2H37A Bleeding Heart plasmid was assembled from the digested pBH and 5xUAS-TATA:M2H37A PCR product using Gibson Assembly Master Mix (NEB). The entire plasmid was sequenced to confirm ligation fidelity.
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bioRxiv - Cell Biology 2019Quote: ... were modified based on the pcDNA3.1(+)-C-I30-FLG-TEV plasmid via the Q5 Site-Directed Mutagenesis Kit (cat#: E0554S, NEB). pCMV-FLAG-ABCE1 was a gift from Dr ...
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bioRxiv - Bioengineering 2019Quote: ... plasmid pRM03-Spec was linearized by AgeI and simultaneously dephosphorylated by Shrimp Alkaline Phosphatase (New England Biolabs, Cat. # M0371S). A 4.2 kb PCM expression cassette was PCR amplified form plasmid pPCM using primer pair MMorCF-AgeIfor/newPCFAgeIrev and double digested with DpnI/AgeI ...
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bioRxiv - Biochemistry 2019Quote: ... Methylation assays were performed using 100 ng of an unmethylated pUC19 plasmid purified from dam- /dcm- E.coli strain (C2925I, NEB) or a 1-kb fragment containing 14 CpG sites amplified from the Meis1 enhancer were used as DNA substrates for filter binding assays ...
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bioRxiv - Biophysics 2019Quote: Double-stranded DNA was synthesized through PCR amplification of a 1.565-kbp segment of the pBR322 plasmid (New England Biolabs), using forward and reverse primers conjugated with a 5’ biotin and a 5’ digoxigenin ...
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bioRxiv - Cancer Biology 2019Quote: A deletion between amino acid 336 and the beginning of GFP was generated from the MCSV:GPR68-GFP plasmid using the Q5 mutagenesis kit (NEB).
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bioRxiv - Synthetic Biology 2019Quote: ... A Gibson assembly was carried out to link all DNA fragments and assemble the final integrative pHO_pCMV_CggR_ble plasmid using the NEB Gibson assembly kit (New England Biolabs, MA, US). 5 µl of reaction mix was transformed into chemical competent E.coli DH5alpha cells ...
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bioRxiv - Biochemistry 2021Quote: ... Yeast surface display plasmid pJYDC1 (Adgene ID: 162458) and pJYDC3 (162460) were cleaved by NdeI and BamHI (NEB, USA) restriction enzymes ...
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bioRxiv - Cell Biology 2021Quote: ... clr4 gene specific sgRNA was cloned into Cas9 containing pLSB-KAN plasmid by Golden Gate Assembly kit (E1601S, NEB). The resulting plasmid clr4-pLSB-KAN and clr4 HR template obtained by annealing primer pair WW748-clr4-HR-F/WW749-clr4-HR-R (Table S3 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The 36N plasmid DNA library was generated using a Q5 site directed mutagenesis kit (New England Biolabs, Ipswitch, MA). For amplification ...
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bioRxiv - Molecular Biology 2021Quote: ... and KpnI) into pSB/IR-CA-HybIntr-SV40intr-BleoR-T2A-mCherry plasmid restricted with restriction endonucleases BsaI (NEB, USA) and AsiG.
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bioRxiv - Bioengineering 2021Quote: ... 20 ng of extracted plasmid DNA was added to a 50 µL PCR reaction with Q5 DNA polymerase (NEB) for 16 cycles annealing at 53 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmid pES101 (encoding GPx7) was constructed by assembling three DNA fragments using the Gibson Assembly Kit (New England Biolabs). The DNA fragments used were a fragment amplified from pcDNA3.1+ (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... PCR fragments were inserted into linearized NaV channel expression plasmids by Gibson Assembly using NEBuilder HiFi Assembly Mix according to the manufacturer’s protocol (New England BioLabs). Introns were inserted after c.2946 (exon 15/16 junction ...
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bioRxiv - Genomics 2021Quote: ... non integrated plasmids were cut by 2 h digestion at 37 °C with I-CeuI restriction enzyme (NEB, R0699S) in a total volume of 70 ul followed by 20 minutes heat inactivation at 65 °C ...
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bioRxiv - Cell Biology 2021Quote: ... The pDD162 plasmid was used to deliver Cas9 and sgRNAs modified using the Q5 Site-Directed Mutagenesis Kit (NEB) for sequence specific CRISPR editing of each gene ...
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bioRxiv - Plant Biology 2021Quote: ... and eGFP were cloned into the digested plasmid according to NEBuilder HiFi DNA Assembly protocol (New England Biolabs, USA). The assembled entry construct was then cloned into the broad host-range vector pHM1 by the Gateway cloning (Supplemental Figure 2 ...
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bioRxiv - Systems Biology 2021Quote: ... Each construct was then randomly barcoded by digesting the plasmid with XbaI followed by HiFi assembly (New England Biolabs) with a single-stranded oligo containing 16 random N’s (TRIP barcodes ...
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bioRxiv - Microbiology 2021Quote: ... were used to amplify the pQE60- ompA recombinant plasmid (Size- 4.5 kb) using Phusion high fidelity DNA polymerase (NEB). The reaction mixture was heated at 950C for 10 minutes for the plasmid’s denaturation ...
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bioRxiv - Microbiology 2020Quote: ... 107 epimastigotes were electroporated with 2.5 µg of pTRIX2-GFP plasmid (Fig 1 A) linearized with AscI and SacI (NEB). The plasmid had been derived from pTRIX-REh9 [28] ...
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bioRxiv - Cancer Biology 2020Quote: ... The sgRNAs were then ligated with the digested plasmid using the T4 DNA ligase and buffer (New England Biolabs). Reactions were carried out following the Zhang Lab General Cloning Protocol (Addgene).
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bioRxiv - Biophysics 2020Quote: ... The point mutants were subsequently cloned in the pNZopuAHis plasmid (C-terminal 6-HIS-tag) using EcoRV-AlnwnI (NEB) for the substrate-binding domain mutants and βamHI-AlwnI (NEB ...
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bioRxiv - Biophysics 2020Quote: ... T270 plasmid was digested by HpaI at 37°C for 4 hr in the CutSmart buffer (New England BioLabs) to place the (TTAGGG)270 at the middle region of the linearized substrate ...
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bioRxiv - Genomics 2022Quote: ... and cloned into the STARR-seq plasmid with NEBuilder® HiFi DNA Assembly Cloning Kit (E5520S, New England Biolabs) per manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... The pLVX-StrepII-ORF-IRES-Puro plasmids were then cloned using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) from the pTriEX-6 plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... P301L/V337M)-EYFP plasmids after the Tau sequence using the Q5 site directed mutagenesis (SDM) kit (New England Biolabs). Tau fragments were subcloned into pcDNA3.1 by restriction digestion and further into pCW57.1- MAT2A all-in-one tet-off lentiviral backbone (a gift from David Sabatini (Addgene plasmid # 100521))71 by Gibson assembly ...
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bioRxiv - Cancer Biology 2022Quote: ... Three sgRNAs designed against Rbms3 were cloned into the pLL3.3 sgRNA-CRE vector by modifying the original sgLKB1 plasmid with the Q5 Site-Directed Mutagenesis kit (NEB). The following sgRNAs against Rbms3 were used and pooled to make 2 lentiviruses.
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bioRxiv - Biochemistry 2022Quote: ... 1x arrays DNA were cut from carrier plasmid DNA by incubation overnight with 5,000 units of EcoRV-HF restriction endonuclease (New England Biolabs) in 1x CutSmart Buffer (20 mM Tris,OAc ...
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bioRxiv - Synthetic Biology 2022Quote: ... in which 20-50 ng of uncut acceptor plasmid and a 2:1 molar ratio of donor plasmid or PCR products were mixed together with 1.5 μl DNA T4 ligase buffer (#B0202S; NEB), 0.0015 mg BSA (B9000S ...
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bioRxiv - Synthetic Biology 2022Quote: ... spacer sequences were cloned into pCAST and pHELIX plasmids via Golden Gate assembly with SapI (New England Biolabs, NEB). Target site features for all gRNAs used in this study are found in Supplementary Table 2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... spacer sequences were cloned into pCAST and pHELIX plasmids via Golden Gate assembly with SapI (New England Biolabs, NEB). Target site features for all gRNAs used in this study are found in Supplementary Table 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... were generated from previously described pcDNA3-FLAG-MS2-TTP plasmids (28) using site-directed mutagenesis according to manufacturer’s protocol (New England Biolabs). Expression plasmids for FLAG-tagged constitutive active and catalytically dead MK2 kinases were previously described (12 ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids encoding Cdiff RNAP mutants (rpoC K84E, K84R, and K84Q) were constructed by Q5 site-directed mutagenesis (NEB) using pXC.026 as the template.
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bioRxiv - Biochemistry 2022Quote: ... Cleavage reactions were performed by combining TtAgo complexes with either 79.85 fmol of linearized KAC833 plasmid substrate (digested with PvuI, NEB) or 79.85 fmol supercoiled plasmid DNA (KAC1151 or MNW95 ...