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Citations for New England Biolabs :
1401 - 1450 of 2232 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Standard curve was prepared using SARS-CoV-2 Positive Control plasmid containing full nucleocapsid protein (N gene) (NEB) and used to quantify copies of N gene in organoid samples ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plasmids were either created by molecular restriction cloning or by the Gibson Assembly technique (New England Biolabs, Inc.). HA-PTH1RmTurquoise/mCitrine (PTH1RFRET ...
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bioRxiv - Genomics 2020Quote: ... 1993) and transformation efficiencies were routinely evaluated using a standard batch of the commercial supercoiled plasmid pLITMUS38 (NEB). All E.coli strains used were obtained from New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: Expression plasmids (Supplementary Table T 2) were cloned by isothermal assembly using NEBuilder HiFi DNA Assembly Mix (NEB). Plasmids were transformed into E ...
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bioRxiv - Cell Biology 2021Quote: ... the myc tag was removed from the Ccz1-myc plasmid using NEB site-directed mutagenesis (SDM) kit (NEB) following manufacturer’s instructions and primers selected using NEBaseChanger tool (table 1) ...
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bioRxiv - Genomics 2021Quote: ... Resulting supercoiled plasmid is linearized at the 3’ end of the PolyA tail using BamHI-HF (NEB R3136S), and checked for reaction completion by running on agarose gel ...
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bioRxiv - Genetics 2020Quote: ... The mutant Tile 3 plasmid pool was then subcloned into the AttB-KCNH2-HA:IRES:mCherry plasmid by restriction digest with BglII and NdeI (NEB), ligated with T4 ligase (NEB) ...
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bioRxiv - Neuroscience 2022Quote: DNA plasmids were made using conventional molecular biology methods and restriction enzymes (RE; New England BioLabs, Ipswich, MA). An adeno-associated virus serotype 2 (AAV2)-based transfer vector with the human synapsin I promoter (hSyn ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids encoding individual variants were pooled and electroporated into electro-competent NEB10β cells (New England Biolabs, Ipswitch, MA), which were then grown in liquid culture overnight and purified using the ZymoPure endotoxin-free midiprep kit (Zymo Research ...
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bioRxiv - Biochemistry 2022Quote: CSF3R constructs were cloned into an MSCV-IRES-GFP (MIG) plasmid digested with XhoI (New England Biolabs R0146S). For the constructs with truncated domains ...
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bioRxiv - Bioengineering 2022Quote: The plasmid backbone was linearized by sequential digestion with SmaI and SpeI (New England Biolabs, Ipswich, MA, USA), and gel purified from 0.8% SeaPlaque GTG Agarose (Lonza ...
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bioRxiv - Immunology 2022Quote: ... The gBlocks were inserted into a pcDNA 3.1+ Zeocine plasmid using Gibson assembly master mix (New England Biolabs). The cDNA construct encoding LAIR-1 ectodomain (sLAIR-1ecto ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli NEB® 5-alpha was used for plasmid assembly and cloning following the manufacturer’s instructions (NEB). Transformed E ...
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bioRxiv - Molecular Biology 2023Quote: ... linearized plasmid by performing an in vitro transcription with SP6 using the Hiscribe SP6 RNA kit (#E2070S, NEB) and a cap analog from the ARCA kit (#S1411 ...
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bioRxiv - Biochemistry 2023Quote: Site directed mutagenesis for I72W M.TaqI mutation on M.TaqI plasmid a was carried out using Q5 Site-Directed Mutagenesis Kit (NEB) by following kit protocol and expressed ...
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bioRxiv - Microbiology 2022Quote: ... Inserts were ligated into the linearized plasmid overnight at 16°C using T4 DNA ligase (New England Biolabs) following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2023Quote: ... The plasmids for this work were constructed using the Gibson-Assembly technique (NEBuilder protocol, New England Biolabs, Germany). Plasmid constructions were first established in E ...
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bioRxiv - Microbiology 2023Quote: ... Constructs for plasmid-based expression of genes were generated using PCR and Gibson Assembly® (NEB, Boston, MA) followed by cloning into pMQ72 ...
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bioRxiv - Microbiology 2023Quote: Promoter DNA fragments were excised from plasmid pSR and end-labelled with γ32-ATP using T4 PNK (NEB). EMSAs and DNase I footprints were done as previously described 40 ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA containing one copy of the SIV gene was linearized using EcoR1 (New England Biolabs, Ipswich, MA) following their restriction digest protocol (New England BioLabs ...
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bioRxiv - Biophysics 2023Quote: ... the CBP-TEV sites was removed from plasmid pRS306 orc1-gal1-10-orc2 through Gibson assembly (NEB #E2611L) using primers TL-441 ...
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bioRxiv - Cancer Biology 2023Quote: ... Mutagenesis of the HA-AHR plasmid was performed using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S).
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bioRxiv - Synthetic Biology 2023Quote: ... All plasmids were generated using standard cloning procedures and the NEBuilder HiFi DNA assembly system (New England Biolabs).
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bioRxiv - Biochemistry 2023Quote: ... All DNA fragments were fused in pCR2.1-TOPO (genome integration cassette) or pGRB2.0 (rescue plasmids) vectors using a Gibson assembly kit (New England Biolabs) and validated by sequencing ...
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bioRxiv - Genetics 2023Quote: ... Plasmids were constructed by Gibson Assembly using NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs Cat. #E2621) and transformed into NEB 10-beta electrocompetent E.coli (New England BioLabs Cat ...
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bioRxiv - Developmental Biology 2023Quote: Plasmids (otxG:Tet-ON 3G; TRE3Gs:Cas9; TRE3Gs:Kaede; U6:sg2; Table S1) were built by Gibson cloning (NEB, Cat# E2611) following standard protocols ...
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bioRxiv - Genetics 2023Quote: ... All PCR amplifications for plasmid and vector constructions were performed using Q5 polymerase (New England Biolabs, Ipswich, MA). Most PCR reactions were performed using the following conditions ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plasmids were constructed in the present using NEB-HiFi DNA assembly master mix from NEB (Beverly, MA).
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bioRxiv - Synthetic Biology 2023Quote: The two plasmids pEKEx2_low_mCherry and pEKEx2_high_mCherry were assembled via Gibson cloning using the Gibson Assembly® Master Mix (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... was subcloned into the pIRES2-dsRED2 plasmid using PCR with ag409 and ag410 and NotI restriction digestion (NEB) to create KCNE1-HA:pIRES2:dsRED2 ...
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bioRxiv - Microbiology 2023Quote: ... plasmid was pre-treated with Alkaline phosphatase and amplicons were treated with T4 polynucleotide kinase (New England Biolabs). Cloned plasmids were subjected to colony PCR using Taq polymerase (New England Biolabs) ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmids encoding chimeric SCS6/MLA1 and SCS6/MLA6 receptors were assembled using the NEBuilder HiFi assembly Kit (NEB) based on the domain boundaries reported in (28) ...
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bioRxiv - Genetics 2023Quote: ... The ints-4 sgRNA was inserted into plasmid pDD162 via the Q5 Site-Directed Mutagenesis Kit (NEB, E0554S). The sgRNA plasmid and repair template were Sanger sequenced to confirm correct construct assembly followed by microinjection into EG9882 strain with integrated Cas9 activity (10 ng µl-1 repair template ...
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bioRxiv - Genetics 2023Quote: All plasmids were generated using Gibson Assembly strategies67 using NEBuilder® HiFi DNA Assembly Master Mix (NEB #E2621) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... the pBOMBL12CRia(ct226) plasmid was modified by SalI-digest and insertion of a gBlock (New England Biolabs (NEB); Cambridge ...
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bioRxiv - Microbiology 2023Quote: Plasmid mAID-5xTy/DHFR/OsTir1_N was constructed by Gibson assembly (NEBuilder® HiFi DNA Assembly Master Mix; NEB) of the following fragments ...
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bioRxiv - Microbiology 2023Quote: ... plasmid backbones without eYFP were amplified by PCR with PR41 and PR42 using Q5 Polymerase (New England Biolabs) and pAJM657 ...
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bioRxiv - Cell Biology 2023Quote: ... pREP81-FLAG plasmid carrying wild-type srs2+ was constructed using the Gibson Assembly® Cloning Kit (NEB E5510S). After Gibson cloning ...
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bioRxiv - Biophysics 2023Quote: A Tandem HaloTag plasmid containing the HaloTag-GTGSGSGS-HaloTag cDNA sequence was built via Gibson Assembly (NEB, (62)) of two HaloTag genes ...
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bioRxiv - Molecular Biology 2023Quote: ... The circularization of the plasmids was then achieved using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520). The primers utilized for site-specific mutagenesis are presented in Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... the pBOMBL12CRia(ct226) plasmid was modified by SalI-digest and insertion of a gBlock (New England Biolabs (NEB); Cambridge ...
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bioRxiv - Molecular Biology 2023Quote: ... the positive control and each viral mutant plasmid was constructed using the Q5 site-directed mutagenesis kit (NEB) according to manufacturer protocol and verified via whole plasmid sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... We verified that this guide RNA digested the Gbait plasmid in combination with Lba Cas12a (New England Biolabs) in an in vitro reaction (data not shown) ...
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bioRxiv - Cell Biology 2023Quote: All plasmids used in this study were constructed using the NEBulider (New England Biolabs, Ipswich, USA; Cat #E2621L) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... Plasmids were then isolated using the Qiagen Miniprep kit and genotyped by digesting with BsrGI (New England Biolabs) to detect the presence of the TARS1 insert ...
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bioRxiv - Genomics 2024Quote: ... The linearized plasmid library and barcoding oligo were assembled with NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) at a 1:5 molar ratio ...
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bioRxiv - Biochemistry 2023Quote: 16.5 nM of linearized template plasmid was added to IVT reactions in RNA polymerase buffer (New England Biolabs) containing 5 mM of each rNTPs ...
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bioRxiv - Plant Biology 2024Quote: ... Linearization of the homology donor and pKS diaCas9_sgRNA plasmids were performed by KpnI-HF® and NdeI (NEB) restriction enzymes respectively ...
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bioRxiv - Synthetic Biology 2024Quote: Plasmid cloning was performed primarily using standard PCR and restriction enzyme cloning with Phusion DNA Polymerase (NEB #M0530L), restriction enzymes (NEB) ...
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bioRxiv - Biophysics 2023Quote: ... assembled with GST tag from pGEX6P plasmid and inserted into pACEBac1 vector via HIFI DNA assembly kit (NEB). The full length KIF5B in pACEBac1 was replaced with a C-terminal TEV-Twin-Strep-tag via HIFI DNA assembly kit (NEB) ...