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Citations for New England Biolabs :
1201 - 1250 of 2029 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... coli Poly(A) Polymerase (New England Biolabs), following the manufacturers’ protocol ...
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bioRxiv - Genetics 2024Quote: ... coli 10β cells (New England BioLabs, C3019I). Following heat-shock ...
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bioRxiv - Microbiology 2024Quote: ... coli protein synthesis system (New England Biolabs) was used for in vitro protein expression ...
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bioRxiv - Biochemistry 2023Quote: ... coli inorganic PPase (New England Biolabs, Inc). All reagents were purchased from Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... coli DNA polymerase I (NEB, cat.m0209, USA), RNase H (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... coli (High Efficiency) (New England Biolabs C2984H) and purified using the NucleoBond Xtra Maxi EF kit (Machery Nagel 740424).
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bioRxiv - Molecular Biology 2021Quote: ... SET8 recombinant enzyme (New England Biolabs # M0428S) was incubated with recombinant active PARP1 (Trevigen # 4668-100-01 ...
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bioRxiv - Molecular Biology 2023Quote: ... 3µl recombinant AsiSI endonuclease (10U/µL, NEB) was added to three biological replicates and incubated (2 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... and recombinant shrimp alkaline phosphatase (rSAP, NEB) overnight and PCR purified (Qiagen QIAQuick PCR Purification Kit ...
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bioRxiv - Cell Biology 2023Quote: Recombinant human Casein Kinase 2 (NEB, P6010S) and Protein Kinase A (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... and recombinant shrimp alkaline phosphatase (all NEB) at 37°C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... 0.2 mg/mL recombinant albumin (NEB B9200S), 1 μM RT primer ...
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bioRxiv - Biochemistry 2019Quote: MBP-tagged TbRGG1 was cloned into a pMAL-c2X vector (NEB) using BamHI and SalI restriction sites ...
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bioRxiv - Plant Biology 2023Quote: ... SNAP-tagged proteins were labelled with Cell-SiR647 (NEB, Frankfurt, Germany). Labeling was done at RT for 1hr ...
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bioRxiv - Systems Biology 2024Quote: Each tagged-ORF plasmid subpool was treated with I-SceI (NEB) in the provided buffer (at a volume of 50uL for ∼1ug hORF plasmids and 200uL for ∼4ug vORF plasmids) ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... MBP-CENP-E was cloned into pMal-C2X (NEB). Bacterially-expressed constructs of GST-CENP-E were cloned in pET-3aTr (Tan ...
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bioRxiv - Microbiology 2019Quote: ... or Bam HI and Xho I (New England BioLabs, MA). The column isolated DNA fragments were ligated into our modified pFastBac1 plasmid having 6xHis tag at C-terminus ...
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bioRxiv - Molecular Biology 2020Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 (Illumina).
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bioRxiv - Microbiology 2021Quote: ... Followed by subcloning the PCR amplicon in Bam-HI (NEB) restriction digested pCW57-tGFP-2A-MCS plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... using NEBuilder Hi-Fi Assembly (New England Biolabs, Cat. #E2621). The pMB1052 construct was subsequently cut using BspDI and EcoRV (New England Biolabs) ...
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bioRxiv - Genomics 2022Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... The Hi-Scribe T7 High Yield RNA synthesis kit (NEB) was used to generate RNA transcripts at 37°C for 16 hours ...
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bioRxiv - Systems Biology 2024Quote: ... using the NEBuilder Hi-Fi Assembly kit (New England Biolabs). To ensure representation of all variants in the population ...
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bioRxiv - Cancer Biology 2023Quote: ... cut with hi-fidelity EcoRI and BamHI (New England Biolabs), and gel purified ...
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bioRxiv - Genomics 2021Quote: ... coli BL21 DE3 cells (C2527, New England Biolabs) using the DNEasy Blood and Tissue kit (69504 ...
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bioRxiv - Microbiology 2021Quote: ... coli strain MG1655 (ATCC 47076) and DH5α (NEB) were grown in LB or MMB (LB supplemented with 0.1 mM MnCl2 and 5 mM MgCl2 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli Stbl (New England Biolabs, Ipswich, MA, USA) for in vivo cloning as previously described27 ...
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bioRxiv - Biophysics 2022Quote: ... coli BL21 (DE3)-pLyse S (NEB, Catalogue # C3010I) cells ...
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bioRxiv - Biochemistry 2020Quote: ... Escherichia coli strain NiCo21(DE3) (New England Biolabs) was transformed with the pLysS plasmid (Novagen ...
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bioRxiv - Biochemistry 2019Quote: ... coli T7 Express (New England Biolabs, Ipswich, USA). For small-scale expression and purification of amounts sufficient to analyze protein splicing in cis and trans ...
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bioRxiv - Microbiology 2019Quote: ... coli DH5α (New England BioLabs, Ipswich, MA, USA) were used in this study ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21(DE3) cells (New England Biolabs C2527) with each plasmid and spread the transformed cells onto an agar plate (25 g/L LB ...
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli NEB 10-beta (New England Biolabs) was used for plasmid preparation and grown in medium supplemented as instructed by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21(DE3) (New England Biolabs, Frankfurt, Germany). Cells were grown in LB medium supplemented with 50 μg ml−1 kanamacin to an OD600 of 0.75 at 37°C and vigorous shaking ...
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bioRxiv - Bioengineering 2021Quote: ... coli cells (New England Biolabs, Cat No: C3029J).
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bioRxiv - Bioengineering 2020Quote: ... coli SHuffle® cells (New England Biolabs, USA) carrying our plasmids of interest were used for recombinant protein expression and were grown using Terrific Broth (TB ...
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bioRxiv - Biochemistry 2021Quote: ... coli strain NiCo21(DE3) (New England Biolabs, NEB) transformed with plasmid pIA900 (31 ...
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bioRxiv - Biochemistry 2021Quote: ... coli strain NiCo21(DE3) (New England Biolabs, NEB) transformed with plasmid pIA900 (31 ...
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bioRxiv - Neuroscience 2019Quote: ... coli (New England BioLabs Inc., Ipswich, Massachusetts, USA). Bacteria were grown overnight with the plasmid of interest in Lysogeny Broth (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: Escherichia coli strain BL21 (DE3) (New England Biolabs) chemically competent was transformed by heat-shock with the recombinant plasmid pET28b-AmDODA to express the C-terminal His-tagged AmDODA ...
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bioRxiv - Immunology 2021Quote: ... coli cells (NEB, catalog no C3040H, 30°C). To generate gp70 C.1086 V1V2 RQ(H/Y)173 constructs ...
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bioRxiv - Plant Biology 2021Quote: The Escherichia coli strain Dh5α (New England Biolabs) was used for cloning purposes ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli DH10B (New England BioLabs, Ipswich, MA, USA) was used for all routine cloning and directed evolution ...
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bioRxiv - Microbiology 2021Quote: ... coli as per manufacturer’s protocol (New England Biolabs) and plated on LB medium supplemented with 100 μg/ml ampicillin ...
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bioRxiv - Microbiology 2021Quote: ... coli were based on pUC19 (New England Biolabs), pET28a-c (cloning with NH2-terminal 6His tag ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli strains DH5αZ1 and NEB10β (New England Biolabs). The different biosensors were grown in LB media with corresponding antibiotics (kanamycin 25 μg/mL or chloramphenicol 25 μg/mL) ...
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bioRxiv - Microbiology 2022Quote: ... coli T7 Express competent cells (Dcm-, NEB #C2566) and plated on agar plates with ampicillin selection (100 μg/ml) ...
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bioRxiv - Bioengineering 2022Quote: ... coli NEB5α (New England Biolabs, Ipswich, MA, USA) competent cells.
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bioRxiv - Biophysics 2022Quote: ... coli BL21(DE3) competent cells (NEB Cat#C2527). Cells were grown at 30°C to an OD of 0.8 ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 T7 Express lacIq (New England Biolabs) from the pLIC-trPC-HMA plasmid with an N-terminal hexahistidine-maltose binding protein (HisMBP ...