Labshake search
Citations for New England Biolabs :
1301 - 1350 of 4746 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... Both the synthesized genes and PCR amplified genes were then inserted into a doubly digested BamHI/XhoI pET28b(+) through Gibson assembly using the 2X Gibson Assembly Master Mix (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2022Quote: ... 25μL NEB Next HiFi PCR Mix (2x solution, NEB M0544), 10μl H2O) ...
-
bioRxiv - Microbiology 2023Quote: ... 12.5 µL 2x Q5 Hot-start Ready mix (NEB, USA), and 8.5 µL ultra-pure water ...
-
bioRxiv - Microbiology 2023Quote: ... Region of Cori and ter were quantitatively amplified using the Sybr green-based Luna Universal One-step RT-qPCR kit (New England Biolabs Inc.), without the reverse transcriptase enzyme ...
-
bioRxiv - Microbiology 2021Quote: ... After digestion the plasmid vector and insert were added to Gibson assembly master mix (1.5 µl insert, 0.5 µl vector, 2 µl master mix) (New England BioLabs) and incubated at 50 °C for 1 hr ...
-
bioRxiv - Cell Biology 2020Quote: ... using Gibson Assembly master mix (NEB E2611L). The resulting vector was digested with SpeI (NEB R0133S ...
-
bioRxiv - Genomics 2019Quote: ... NEBNext Ultra II Q5 Master Mix (NEB) was used to amplify diluted poly(G/I ...
-
bioRxiv - Genomics 2021Quote: ... 25 µL 2× Q5 master mix (NEB); 1 µL 10 µM TruSeq PCR handle primer (IDT) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... or Golden Gate master mix (NEB #E1601). Plasmids were incubated on ice for 30 min with competent cells which were prepared using CCMB80 buffer (TekNova ...
-
bioRxiv - Cancer Biology 2020Quote: ... 5μl Phusion High-Fidelity master mix (NEB). The sample was run on the CFX96 real-time PCR system (Bio-Rad ...
-
bioRxiv - Biochemistry 2022Quote: ... using HiFi DNA Assembly Master Mix (NEB) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... The HiFi Assembly reaction master mix (NEB) was used according to the manufacturer’s instructions in conjunction with plasmids pBOMB4-Tet (kind gift of Dr ...
-
bioRxiv - Biophysics 2022Quote: ... Using Gibson Assembly Master Mix (NEB, USA), the two purified flanking PCR fragments (Promega ...
-
bioRxiv - Microbiology 2020Quote: ... were performed using Taq Master Mix (NEB) as per the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... using Gibson Assembly® Master Mix (NEB). Positive clones were analysed by Sanger sequencing ...
-
bioRxiv - Cell Biology 2022Quote: ... with Luna Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... The HiFi Assembly reaction master mix (NEB) was used according to the manufacturer’s instructions in conjunction with plasmids pASK-GFP-mKate2-L2 (kind gift of Dr ...
-
bioRxiv - Cell Biology 2019Quote: ... Gibson Assembly Master Mix (New England Biolabs) was used according to manufacturer’s protocol ...
-
bioRxiv - Zoology 2020Quote: ... 1X OneTaq Hot Start Master Mix (NEB) and 0.2 µM each primer in a total volume of 25 µl ...
-
bioRxiv - Zoology 2020Quote: ... 1X OneTaq Hot Start Master Mix (NEB), 0.4 µM each primer in total volume of 25 µl ...
-
bioRxiv - Zoology 2020Quote: ... 1X OneTaq Hot Start Master Mix (NEB) and 0.2 µM each primer in a total volume of 25 µl ...
-
bioRxiv - Zoology 2020Quote: ... 1X OneTaq Hot Start Master Mix (NEB), 0.2 µM each primer in a total volume of 25 µl ...
-
bioRxiv - Immunology 2020Quote: ... Canada) using Gibson Assembly Master Mix (NEB) for 45 minutes at 50°C ...
-
bioRxiv - Plant Biology 2020Quote: ... 25 μL master mix (from NEB kit), and 1 μL each of a 25 μmol/L solution of each forward and reverse primer (forward 5′-AAT GAT ACG GCG ACC ACC GAG ATC TAC ACT CTT TCC CTA CAC GAC GCT CTT CCG ATC* T-3′ ...
-
bioRxiv - Genomics 2021Quote: ... using its dedicated master mix (NEB, UK) and custom-designed primers ...
-
bioRxiv - Microbiology 2020Quote: ... The HiFi Assembly reaction master mix (NEB) was used according to the manufacturer’s instructions in conjunction with plasmids pASK-GFP-mKate2-L2 (kind gift of Dr ...
-
bioRxiv - Neuroscience 2022Quote: ... Phusion HF PCR Master Mix (1x, BioLabs), water (6.42 µL) ...
-
bioRxiv - Molecular Biology 2022Quote: ... or Gibson Assembly Master Mix (NEB E2611L) to insert deaminase regions into pICE backbone (Addgene #46960) ...
-
bioRxiv - Cell Biology 2023Quote: ... Q5 PCR Master Mix (New England BioLabs) was used for PCR reactions ...
-
bioRxiv - Genomics 2023Quote: ... ligation master mix was added (10x NEB T4 DNA ligase buffer with 10 mM ATP B0202-NEB) ...
-
bioRxiv - Genetics 2023Quote: ... NEBNext High Fidelity Master Mix (NEB, M0541L), and 2.5 µg DNA for each reaction ...
-
bioRxiv - Systems Biology 2023Quote: ... and the Gibson Assembly Master Mix (NEB). We used the DNA Clean & Concentrator Kit (Zymo Research ...
-
bioRxiv - Molecular Biology 2023Quote: ... or Phusion High-Fidelity Master Mix (NEB). Backbone plasmids were linearised via PCR followed by treatment with DpnI (NEB ...
-
bioRxiv - Pathology 2023Quote: ... A Blunt/TA Ligase Master Mix (NEB) was then used to ligate the sequencing adapter (AMX ...
-
bioRxiv - Synthetic Biology 2023Quote: NEBuilder HiFi DNA Assembly Master Mix (NEB) was used for all assembly reactions ...
-
bioRxiv - Molecular Biology 2023Quote: ... or Gibson Assembly Master Mix (NEB E2611L). Assembled plasmids were transformed into Mach1 (Invitrogen #C862003 ...
-
bioRxiv - Microbiology 2023Quote: ... Using the Gibson Assembly Master Mix (Biolabs), purified PCR fragments were cloned into the pMSR plasmid linearized by inverse PCR and treated by DpnI ...
-
bioRxiv - Genomics 2023Quote: Blunt/TA Ligase Master Mix (NEB M0367S)
-
bioRxiv - Microbiology 2023Quote: ... Gibson Assembly® Master Mix (NEB #E2611) was used for all Gibson assembly reactions following the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... cDNA template and LUNA master mix (NEB) were added into white PCR reaction tubes and placed in a CFX Maestro (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2024Quote: ... ligated with Blunt/TA master mix (NEB) or DNA ligation kit (Takara ...
-
bioRxiv - Biochemistry 2024Quote: ... using HiFi DNA Assembly Master Mix (NEB) following the manufacturer’s instructions (Supplementary Table 3) ...
-
bioRxiv - Biochemistry 2024Quote: ... NEBuilder HiFi DNA Assembly Master Mix (NEB) was used for subcloning ...
-
bioRxiv - Biochemistry 2024Quote: ... NEBuilder HiFi DNA Assembly Master Mix(NEB) was then used to insert them into 6x histidine tagged PanZ(HTSD1.10 ...
-
bioRxiv - Neuroscience 2021Quote: ... Quantitative real-time PCR was performed with 20 ng of cDNA in a total reaction volume of 10 μL using Luna® Universal qPCR Master Mix (Cat. No. M3003L; New England BioLabs) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... Gene expression to validate RNA-seq analysis was quantified by qRT-PCR using Luna® Universal qPCR Master Mix (New England Biolabs) on a QuantStudio 3 real-time PCR system (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: ... Expression profiles of the genes listed below were analyzed with the specified primers using Luna® Universal qPCR Master Mix (NEB). Quantitative RT-PCR was performed using StepOnePlus (Applied Biosystems) ...
-
bioRxiv - Genetics 2020Quote: ... Each reaction was run in 10μL volumes and included 1x Luna Universal qPCR Master Mix (New England BioLabs, Ipswich, Mass. USA), optimal forward and reverse concentrations were experimentally determined (200nM for both forward and reverse primers for 17S ...
-
bioRxiv - Immunology 2021Quote: ... 10 µL diluted cDNA was used with the NEB Luna® Universal Probe qPCR Master Mix (New England BioLabs® Inc.) following the manufacturer’s protocol and performed in 384-well plates using a QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™) ...
-
bioRxiv - Genomics 2021Quote: ... in a 20 μl reaction mixture consisting of Luna® Universal qPCR Master Mix (New England Biolabs, Inc., Cat. No.: NC1276266), forward and reverse primer pairs (F515-GTGCCAGCMGCCGCGGTAA and R806-GGACTACHVGGGTWTCTAAT ...