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Citations for New England Biolabs :
1451 - 1500 of 4746 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... using Q5 High-Fidelity Master Mix (New England Biolabs) were cloned into pSW-I digested with ScaI.16
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bioRxiv - Molecular Biology 2022Quote: ... and 12 µL NEB master mix (NEB, United Kingdom). Nuclease free water (GeNei ...
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bioRxiv - Molecular Biology 2022Quote: ... 25 µl NEBNext Ultra II Q5 Master Mix (NEB) and indexed amplification primers (125 nM final concentration ...
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bioRxiv - Biochemistry 2022Quote: ... Gibson Assembly Master Mix was purchased from NEB (USA). Amicon Ultra-0.5 mL centrifugal units and Benzonase® nuclease were purchased from MilliporeSigma (USA) ...
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bioRxiv - Immunology 2022Quote: ... vector by using Gibson Assembly Master Mix (NEB, E2611S). The gRNA expression vector MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP was obtained from Addgene (86457) ...
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bioRxiv - Genomics 2019Quote: ... and 50 µl Blunt/TA ligase master mix (NEB) were combined and incubated at room temperature for 10 minutes ...
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bioRxiv - Genomics 2019Quote: ... using NEBuilder® HiFi DNA Assembly Master Mix (NEB).
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bioRxiv - Genomics 2020Quote: ... Phusion Hot-start master mix (New England Biolabs, M0536L), DNase I (New England Biolabs ...
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bioRxiv - Biophysics 2019Quote: ... using NEBuilder® HiFi DNA Assembly Master Mix (NEB) along with a tandem 6x polyhistidine tag separated by a GSG linker.
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bioRxiv - Synthetic Biology 2021Quote: ... using NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621). ΔVP1 was codon-optimised for yeast and synthesised by GenScript ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.5 vol 2×Phusion Master Mix (New England Biolabs), and 0.04 vol P1 and P2 amplification primers (10 nm) ...
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bioRxiv - Microbiology 2020Quote: ... 1X Phusion Hot Start Flex Master Mix (NEB; M0536L) and H2O up to a total volume of 25 μl ...
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bioRxiv - Microbiology 2020Quote: ... 1X Phusion Hot Start Flex Master Mix (NEB; M0536L) and H2O up to a total volume of 50 μl ...
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bioRxiv - Plant Biology 2020Quote: ... 5 µl Taq 5× Master Mix (New England Biolabs) and 18 µl Milli-Q water (18.2 MΩ cm) ...
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bioRxiv - Bioengineering 2021Quote: ... NEBuilder Hifi DNA Assembly Master Mix (New England Biolabs) was used ...
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bioRxiv - Microbiology 2019Quote: ... and Gibson assembly reagents (NEB HiFi Assembly Master Mix) for generation of recombinant DNAs and for routine molecular cloning and subcloning procedures were obtained from New England Biolabs (Ipswitch ...
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bioRxiv - Microbiology 2019Quote: ... per reaction: Phusion Master Mix (New England BioLabs, Inc.), 3% final volume DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... using the Gibson Assembly Master Mix (New England BioLabs).
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bioRxiv - Microbiology 2021Quote: ... Q5 Hot Start High-Fidelity Master Mix (NEB #M0494), TSO-specific primer ...
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bioRxiv - Genomics 2019Quote: ... 25μL of LongAmp Taq master mix (New England Biolabs) and 1μL of PCR barcode (ONT) ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions were prepared with OneTaq Master-mix (NEB) in 96 well plates to a final volume of 20μl ...
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bioRxiv - Microbiology 2022Quote: ... using the Gibson Assembly Master Mix (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... NEBuilder HiFi DNA Assembly DNA Master Mix (E2621L, NEB) was used ...
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bioRxiv - Cell Biology 2022Quote: ... and combined using HiFi® Assembly Master Mix (NEB). Point mutations were made using the Q5 Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Microbiology 2022Quote: ... All cloning was performed with Q5 Master Mix (NEB) and NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Cell Biology 2023Quote: ... 25 μL NEBNext HiFi 2 × PCR Master mix (NEB) was added ...
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bioRxiv - Synthetic Biology 2023Quote: ... we used the Gibson Assembly® master mix (NEB) with PCR products amplified from pErv3 and custom gene fragments (IDT gBlocks™ ...
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bioRxiv - Molecular Biology 2023Quote: ... All PCRs utilised Q5 High-Fidelity Master Mix (NEB) or Phusion High-Fidelity Master Mix (NEB) ...
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bioRxiv - Genetics 2023Quote: ... OneTaq DNA polymerase master mix (New England Biolabs #M0489S), and a T100 Thermal Cycler (Biorad #1861096) ...
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bioRxiv - Molecular Biology 2023Quote: ... The LongAmp Taq Master Mix [New England Biolabs (NEB)] and ONT PR2 Primer were used to synthetize the second strand of cDNAs ...
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bioRxiv - Synthetic Biology 2023Quote: ... using the NEBuilder HiFi DNA Assembly Master Mix (NEB). 20 uL reactions were incubated at 50C for 1 hour to increase assembly yield ...
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bioRxiv - Cancer Biology 2023Quote: ... Blunt/TA Ligase Master Mix (New England Biolabs, M0367S); NEBNext Quick Ligation Reaction Buffer (New England Biolabs ...
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bioRxiv - Bioengineering 2024Quote: ... Gibson Assemblies (NEBuilder HiFi DNA Assembly Master Mix, NEB) and bacterial transformations (Mix & Go! Competent Cells - DH5 Alpha ...
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bioRxiv - Genetics 2023Quote: ... using NEBNext Ultra II Q5 master mix (NEB M0544S). The PCR product was purified using Amicon Ultra-2mL 50K centrifugal filter (Millipore UFC205024 ...
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bioRxiv - Neuroscience 2024Quote: ... Cloning was done using Gibson Assembly Master Mix (NEB) and the resulting products were cleaned up using SPRIselect beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... using the Gibson Assembly Master Mix (New England Biolabs). pPM28 was similarly generated by Gibson Assembly of the pHlourin amplicon into pPM32 ...
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bioRxiv - Bioengineering 2024Quote: LunaScript Primer-Free RT Master Mix Kit (NEB E3025S) was used to generate cDNA using primers oAS344 for cRNA and oAS345 for vRNA (Supplementary Note 21).
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bioRxiv - Cell Biology 2024Quote: ... using NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621). Proteins were transfected into Rosetta2 (DE3 ...
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bioRxiv - Molecular Biology 2024Quote: ... containing 6,25µl of LongAmp Taq 2✕ Master Mix (NEB), 4,25µl of milliQ water ...
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bioRxiv - Immunology 2021Quote: ... Master mix preparation and cycling conditions were realized with Luna® Universal qPCR Master Mix kit (cat. n° M3003E, NEB, New England Biolabs, Ipswich, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative RT-PCR was conducted on a Roche Lightcycler 480 instrument using Luna Universal qPCR master mix (New England Biolabs in Ipswich, MA, USA). The geometric mean of recA and gyrB transcripts was used to normalise data ...
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bioRxiv - Plant Biology 2023Quote: ... Three cDNA samples derived from three independent RNA extractions were synthesized and amplified using Luna®Universal qPCR Master Mix (New England Biolabs, Ipswich, MA, USA) on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... and 25 μL of 2X PCR mix with Q5 DNA polymerase (NEB #M0491S) (Q5 5X buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Amplification using Luna One Step qPCR mix with UDG (NEB) that did not contain a reverse transcriptase component (NEB ...
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bioRxiv - Genomics 2021Quote: ... Adapter mix (ONT, Oxford, UK) and Blunt/TA Ligation Master Mix (NEB, Ipswich, Ma, USA) were added to the purified end-prepped DNA and incubated for 15 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR amplifications were performed using Phusion or Q5 high fidelity DNA polymerase (NEB Phusion Master Mix with HF buffer #M0531L or NEB Q5 Master Mix # M0492L). MinElute PCR Purification Kit (Qiagen #28004 ...
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bioRxiv - Microbiology 2020Quote: ... Constructs were assembled using the Gibson assembly master mix (NEB) according to manufacturer’s directions and transformed into NEB5α competent cells (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... Constructs were assembled using Gibson master mix (New England Biolabs).
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bioRxiv - Molecular Biology 2020Quote: ... Then the Gibson Assembly® Master Mix – Assembly (NEB, #E2611) was used according to user’s manual ...
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bioRxiv - Cell Biology 2022Quote: ... using NEBuilder® HiFi DNA Assembly Master Mix (NEB, E2621L). To obtain pCMV-mNeonGreen-Sec16A ...