Labshake search
Citations for New England Biolabs :
1351 - 1400 of 4746 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... were incubated with 25 μL of containing TF or 18S primers and fluorescent Luna® Universal qPCR Master Mix (New England Biolabs), and qRT-PCR was carried out in triplicate for each sample on the CFX Connect real-time System (Bio-Rad Laboratories).
-
bioRxiv - Cell Biology 2022Quote: ... Real-time PCR was performed to quantify the expression level of mRNAs by Luna® Universal qPCR Master Mix (NEB, M3003E) and LightCycler ®480 Real-Time PCR System (Roche) ...
-
bioRxiv - Systems Biology 2023Quote: ... was decided by calculating the cycle threshold value from a qPCR reaction using the NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB #M0543L) for the specified cDNA concentration ...
-
bioRxiv - Genetics 2023Quote: ... cDNA was then used as a template for qPCR using the Luna Universal Probe Master Mix (New England Biolabs, Ipswich, Massachusetts) with the Universal Probe Library #21 probe (Roche ...
-
bioRxiv - Genetics 2022Quote: ... and lysates were used as gDNA templates for PCR amplification with OneTaq® Quick-Load® 2X Master Mix with Standard Buffer (all materials from New England BioLabs, Ipswich, MA). Genotyping was performed with primers listed in Supplemental Table 6 with the following thermocycling conditions ...
-
bioRxiv - Immunology 2022Quote: ... This sequence was cloned into pLVX-IRES-ZsGreen1 (TakaraBio) at the EcoRI and NotI sites using Gibson assembly (NEB 2x Gibson Assembly Master Mix). The resulting construct was packaged into a lentivirus using the packaging vectors psPAX2 and pMD2G and the LV-MAX lentiviral production system (ThermoFisher) ...
-
bioRxiv - Genomics 2021Quote: ... and 7 PCR cycles were used to amplified libraries using NEBnext High-Fidelity 2X PCR Master Mix (New England Biolabs, Ipswich MA, Catalog #M0541S) and SYBR Green I (ThermoFisher ...
-
bioRxiv - Bioengineering 2020Quote: ... 2–3 μl of genomic DNA was used as a template in a 50-μL PCR reaction with Q5® High-Fidelity 2X Master Mix (NEB, Ipswich, MA, USA). The following primers (Table S1 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The first round of PCRs was performed in a final reaction volume of 25 μl including 12.5 μl of Q5® Hot-Start High-Fidelity 2X Master Mix (New England Biolabs® Inc., MA, USA), 1.5 µl of each indexed primer (10 µM ...
-
bioRxiv - Microbiology 2023Quote: ... These regions of homology allow the Girr gene sequence to be inserted into pExTra downstream of the pTet promoter by isothermal assembly (NEB HiFi 2X Master Mix) as previously described (Heller et al ...
-
bioRxiv - Microbiology 2023Quote: ... PCR was conducted following the manufacturer’s instructions for the Q5® High-Fidelity 2X Master Mix (Cat. No. M0492S, New England BioLabs, Inc, Ipswich, MA, USA), the following conditions ...
-
bioRxiv - Bioengineering 2024Quote: ... gDNA was extracted using full B2M locus primers (Supplement Table 1) and amplified with Q5® Hot Start 2x Master Mix (New England Biolabs, Ipswich, Massachusetts, US). The target DNA was purified by PureLink® PCR cleanup (Invitrogen) ...
-
bioRxiv - Microbiology 2020Quote: ... Cloning reactions were performed with Q5 High-Fidelity Master Mix and HiFi DNA Assembly Master Mix (New England Biolabs). pORTMAGE312B (Addgene accession ...
-
bioRxiv - Synthetic Biology 2019Quote: ... PCR were performed using Q5 PCR master mix and One-Taq quick load master mix for colony PCR (NEB), primers were purchased from IDT (Louvain ...
-
bioRxiv - Synthetic Biology 2021Quote: ... PCR were performed using Q5 PCR master mix and One-Taq quick load master mix for colony PCR (NEB), primers were purchased from IDT (Louvain ...
-
bioRxiv - Cell Biology 2023Quote: ... were used to amplify hnRNPAB X2 from cDNA using Phusion PCR master mix which was then cloned into pSP64TNRLMCS:mCh10 using Gibson Assembly master mix (New England Biolabs) to generate pSP64:mCh-hnRNPAB-X2 ...
-
bioRxiv - Genomics 2020Quote: ... 25 µl 2x NEBNext High-fidelity PCR mix (New England Biolabs), and 15 µl H2O ...
-
bioRxiv - Genomics 2021Quote: ... and 25 µl 2x NEBNext Hi-Fi PCR mix (NEB, USA) per reaction ...
-
bioRxiv - Immunology 2022Quote: ... and 10 μl 2X Luna Universal One-step Reaction mix (NEB). Samples were measured in triplicates ...
-
bioRxiv - Molecular Biology 2023Quote: ... 39 µL of qPCR mix containing 1× Phusion mix (New England Biolabs, M0531S), 0.125 µM P6 Tall v4 primer ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 20 µL qPCR reactions were carried out in duplicate according to the protocol provided with the Luna Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, MA). Quantification was performed by comparison to a standard curve of E ...
-
bioRxiv - Microbiology 2022Quote: ... DNA was subjected to qRT-PCR analysis using the Luna Universal qPCR Master Mix Protocol (New England Biolabs, Frankfurt am Main, Germany) and a CFX96 Real-Time System ...
-
bioRxiv - Immunology 2023Quote: ... Real-time quantitative PCR was run on the Eppendorf Mastercycler (SybrGreen) using the LUNA Universal qPCR Master Mix (New England Biolabs, Catalog # M3003). Primers used for real-time qPCR can be found in Supplementary Data 5.
-
bioRxiv - Microbiology 2023Quote: ... at a final volume of 20 μL with 10 μL of Luna® Universal qPCR Master Mix (New England Biolabs, Frankfurt, Germany). Each primer was added at a final concentration of 300 nM ...
-
bioRxiv - Molecular Biology 2020Quote: ... by amplification of fragments using Q5 Hot Start High-Fidelity Master Mix and ligation using a Gibson Assembly Master Mix (New England Biolabs). pLKO.1-puro plasmids encoding scrambled shRNA and five FYCO1-targeted shRNAs were purchased from Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2022Quote: ... and mixed with a master mix consisting of 20 μl of 2 x NEBNext Ultra II Q5 Master Mix (NEB), 1 μl of 40 μM P5* primer ...
-
bioRxiv - Systems Biology 2021Quote: ... PCR reactions with plasmid and genomic DNA templates were performed using the Phusion High-Fidelity 2× Master Mix or Q5 High-Fidelity 2× Master Mix (New England Biolabs) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2021Quote: ... DNA was amplified with 12 cycles of PCR using the NebNext Hi-Fi 2X PCR Master Mix (New England Biolabs Inc, Ipswich, MA, Cat #M0541S). Following PCR ...
-
bioRxiv - Molecular Biology 2022Quote: ... Second PCRs were run in duplicate in a final reaction volume of 50 µL using 25 µL of Q5® Hot-Start High-Fidelity 2X Master Mix (New England Biolabs® Inc., MA, USA), 3 µL of each Illumina tag (10 µM ...
-
bioRxiv - Molecular Biology 2020Quote: ... Second PCRs were run in duplicate in a final reaction volume of 50 µl using 25 µl of Q5® Hot-Start High-Fidelity 2X Master Mix (New England Biolabs® Inc., MA, USA), 3 µl of each Illumina tag (10 µM ...
-
bioRxiv - Zoology 2023Quote: Polymerase chain reaction (PCR) products were amplified in a total volume of 25µL with QuickLoad® Taq 2X Master Mix (New England Biolabs Inc., USA, catalogue number M0271L) and 0.2 µM primer for each pair ...
-
bioRxiv - Genetics 2021Quote: ... with NEBuilder HiFi DNA Assembly Master Mix (NEB). The SNP STARRseq capture library was then transformed into MegaX DH10B T1R electrocompetent cells (Invitrogen ...
-
bioRxiv - Microbiology 2022Quote: ... using NEBuilder HiFi DNA Assembly Master Mix (NEB). Primers used to assemble expression plasmids for domain deletion mapping and alanine scanning mutagenesis of CD164 can be found in Table S4.
-
bioRxiv - Molecular Biology 2021Quote: ... 41.7 µL of Phusion 2× Master Mix (NEB), 2.5 µL of 10 µM primer mix (515F forward and 806R reverse rRNA gene V4 primers with Illumina MiSeq adaptors) ...
-
bioRxiv - Molecular Biology 2021Quote: ... NEB Q5 Ultra II Master Mix (NEB #M0544L), KAPA HiFi HotStart (Roche #KK2601) ...
-
bioRxiv - Molecular Biology 2019Quote: ... by NEBuilder HiFi DNA Assembly Master Mix (NEB). For BEX3 and SERBP1 ...
-
bioRxiv - Cancer Biology 2020Quote: ... using Q5 High-Fidelity Master Mix (NEB, M0492) and the following primers ...
-
bioRxiv - Cancer Biology 2019Quote: ... Phusion high fidelity master mix (New England BioLabs) was used with 10 μM of each primer and 1 ng gBlock or 50 ng genomic DNA and amplified for 25 cycles ...
-
Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... using the Gibson Assembly® Master Mix (NEB) according to the manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2019Quote: ... and 1x PCR Master Mix (NEB, Cat#M0544). The samples were thermocycled at 72°C for 5 minutes ...
-
bioRxiv - Microbiology 2019Quote: ... using HiFi Assembly Master Mix (New England Biolabs). For the plasmid pNPTS138cpaF::mCherry-cpaF ...
-
bioRxiv - Cancer Biology 2020Quote: ... a Gibson Assembly Master Mix (New England Biolabs) was used according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2020Quote: ... 1X WarmStart LAMP Master Mix (New England Biolabs), and 0.5 U/μL SUPERase•In™ RNase Inhibitor (Invitrogen) ...
-
bioRxiv - Systems Biology 2020Quote: ... 10 μL Gibson assembly master mix (NEB E2611) and nuclease free water to a final volume of 20 μL then incubated the reaction at 50°C for 1 hour ...
-
bioRxiv - Cancer Biology 2020Quote: ... with NEBuilder HiFi DNA Assembly Master Mix (NEB). The ARBS STARRseq library was transformed into MegaX DH10B T1R electrocompetent cells (Invitrogen) ...
-
bioRxiv - Microbiology 2022Quote: ... and Gibson assembly (Gibson Assembly Master Mix, NEB). Sanger sequencing was carried out to check vector construction and seamless integration into the genome (Eurofins Genomics) ...
-
bioRxiv - Genetics 2022Quote: ... with NEBuilder HiFi DNA Assembly Master Mix (NEB). The SNP STARRseq capture library was then transformed into MegaX DH10B T1R electrocompetent cells (Invitrogen ...
-
bioRxiv - Developmental Biology 2022Quote: ... using with Q5 High Fidelity master mix (NEB) and subcloned into pWPXLd (Addgene #12258 ...
-
bioRxiv - Microbiology 2021Quote: ... Q5 DNA polymerase master mix (New England Biolabs) was used for PCR reactions intended for cloning ...
-
bioRxiv - Synthetic Biology 2020Quote: ... NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621) was used for Gibson Assemblies ...